1.Influence of noninvasive positive pressure ventilation treatment on the brain natriuretic peptide level of patients with pulmonary heart disease
Zhangsheng LI ; Chunyun ZHANG ; Lu SEHN
Chinese Journal of Primary Medicine and Pharmacy 2012;19(4):513-514
Objective To explore influence of noninvasive positive pressure ventilation treatment on the brain natriuretic peptide(BNP) level of patients with pulmonary heart disease.Methods 104 patients with pulmonary heart disease were randomly divided into two groups,control group ( conventional therapy) and study groups ( noninvasive ventilation),each group 52 cases.The BNP level of the two groups before and after treatment were detected and compared.Results After treatment,the BNP level in the two groups were all decreased,and the BNP level of the study group was( 197.8 ± 39.6) ng/L,which was significantly lower than that of the control group[ (320.9 ± 43.0 ) ng/L ]( t =3.22,P < 0.05).Conclusion Noninvasive positive pressure ventilation treatment could effectively reduce BNP level in patients with pulmonary heart disease.
2.Effects of treatment with noninvasive positive pressure ventilation combined with aerosol inhalation budesonide on severe asthma
Zhangsheng LI ; Chunyun ZHANG ; Lu SHEN
Clinical Medicine of China 2012;28(6):612-614
Objective To investigate the curative effect of noninvasive positive pressure ventilation combined with aerosol inhalation of budesonide on patients with severe asthma.Methods One hundred and twenty-one patients with severe asthma were randomly assigned to two groups.Both groups were treated with noninvasive positive pressure ventilation based on routine treatment.In addition,patients in the treatment group were given aerosol inhalation of 2 ml budesonide and 2 ml NS,two times each day,14 days for a course.Clinical symptoms improvement and arterial blood pressure changes of two groups before and after treatment were studied to compare the curative effect of the treatments in the two groups.Results There were no significant differences in pH,PaO2 and PaCO2 between the treatment and the control groups(P > 0.05).After one course of treatment,the treatment group had significantly higher levels of pH(7.43 ± 0.04 vs.7.34 ± 0.02,t =3.423,P < 0.05) and PaO2 ( [77.4 ± 16.7 ] mm Hg vs.[ 62.4 ± 16.5 ] mm Hg,t =3.414,P < 0.05 ) and lower levels of PaCO2 ( [43.4 ±8.9] mm Hg vs.[50.9 ± 9.1 ] mm Hg,t =5.317,P < 0.05 ) than the control group.There were significant differences in the total effective rate between the Budesonide group and the control group (96.7% vs.88.3%,x2 =4.218,P < 0.05 ).Conclusion Noninvasive positive pressure ventilation combined with aerosol inhalation of budesonide is a simple and effective strategy for patients with severe asthma.Thus it may be worthy to be widely used clinically.
3.Clinical signicance of the detection of plasma N-teminal pro brain natriuretic peptid (NT-pro-BNP) level in the patients with different heart failure
Chunyun ZHANG ; Lu SHEN ; Zhangsheng LI
Chinese Journal of Primary Medicine and Pharmacy 2013;20(9):1343-1344
Objective To study the clinical significance of plasma N-teminal pro brain natriuretic peptide (NT-pro-BNP) level in diagnosis and evaluation prognosis of patients with different heart failure.Methods Plasma NT-pro-BNP level was detected and compared in all patients at the first day and a week after the patients' condition turn better.Results The level of plasma NT-pro-BNP for the high blood pressure's heart disease and the chronic heart failure of coronary heart disease and the chronic heart failure of cor pulmonale were significantly higher than normal (t =2.98,t =2.98,t =2.98,P < 0.01).The level of plasma NT-pro-BNP was reduced after treatment (t =2.56,t =2.75,t =2.88,P < 0.05,P < 0.01,P < 0.01).The level of plasma NT-pro-BNP was gradually increased by the increased degree of the heart failure(t =2.78,P < 0.05).Conclusion The levels of plasma NT-pro-BNP at acute stage for the high blood pressure's heart disease and the chronic heart failure of coronary heart disease and the chronic heart failure of cor pulmonale were increased obviously and descend by the patients' condition return.The level of plasma NT-pro-BNP can monitor patients' condition and estimate the heart failure patients' prognosis.
4.Cigarette smoke aggravates sertoli/spermatogenic cells damage by activating the ROS/NLRP3 signaling pathway
Shuomin Wang ; Yifan Hu ; Bingxian Zhou ; Shiheng Fan ; Qisheng Wu ; Yayan Fan ; Chunyun Lu ; Dee Yu ; Jing Zhang
Acta Universitatis Medicinalis Anhui 2023;58(9):1557-1564
Objective :
To investigate the mechanism of sertoli/spermatogenic injury induced by cigarette smoke extract(CSE) based on ROS/NLRP3 signaling pathway.
Methods :
The sertoli/spermatogenic of rat were cultured in vitro , and they were divided into control group , cigarette Smoke extract (CSE) group and CSE + NLRP3 inflammosome inhibitor MCC950 group. Cell viability was detected by CCK⁃8 assay; Lactate dehydrogenase ( LDH) was used to detect cell membrane damage ; reactive oxygen species ( ROS) production was used to detect oxidative stress level ; Hoechst/PI fluorescent staining was used to detect cell pyroptosis. Real⁃time quantitative PCR ( RT⁃qPCR) was used to determine the mRNA level of NLRP3 , ASC , Caspase⁃1 , GSDMD , IL⁃1β and IL⁃18. Western blot was used to determine the protein level of NLRP3 , ASC , Caspase⁃1 , GSDMD , IL⁃1β and IL⁃18.
Results:
CCK⁃8 assay showed that cell viability decreased in CSE group (P < 0. 001) , LDH leakage rate (P < 0. 001) and ROS (P < 0. 001) production increased. Compared to the CSE group , cell viability increased (P < 0. 001) , LDH leakage rate (P < 0. 01) and ROS (P < 0. 001) production decreased in CSE + MCC950 group. The number of PI staining cells in CSE group was more than that in control group and CSE + MCC950 group , but staining cells in CSE + MCC950 group was less than that in CSE group. RT⁃qPCR and Western blot results showed that the expression levels of NLRP3 , ASC , Caspase⁃1 , GSDMD , IL⁃1β and IL⁃18 increased in CSE group but decreased in CSE+ MCC950 group.
Conclusion
The oxidative stress and inflammation of sertoli/spermatogenic induced by ciga⁃ rette smoke may be related to the activation of ROS/NLRP3 signaling pathway.
5.Current situation and related factors of occupational stress of employees of a petrochemical enterprise
Qisheng WU ; Bingxian ZHOU ; Shiheng FAN ; Shuomin WANG ; Yifan HU ; Yayan FAN ; Chunyun LU ; De´e YU ; Lei JIN ; Jing ZHANG
Journal of Environmental and Occupational Medicine 2023;40(7):817-822
Background Occupational stress has become one of the main factors affecting people's physical and mental health, and there are many sources of occupational stress in petrochemical enterprises. Objective To evaluate the current situation of occupational stress and its related factors among employees in a petrochemical enterprise, and to provide a scientific basis for reduing the risk of occupational stress among employees in petrochemical enterprises. Methods In June 2022, a cross-sectional questionnaire survey was conducted in a petrochemical enterprise in Hainan, including a general information questionnaire for basic information, the Effort-Reward Imbalance (ERI) for occupational stress, and the Pittsburgh Sleep Quality Index (PSQI) for sleep quality. Chi-square test was used to compare differences in positive occupational stress by demographic characteristics, occupational characteristics, behavior, and occupational disease hazards. Logistic regression was employed to evaluate factors associated with occupational stress. Results Of the