ObjectiveTo investigate the role of apoptosis played in myocardial hypertrophy and the effect of L-arginine (L-Arg) on the pathogenesis.Methods36 rats were randomly divided into the control group, model group and L-Arg treating group. The animal model of over-loading myocardial hypertrophy was made, and systolic blood pressure (SBP)and the cardiac indexes were measured, spectrophotography and flow cytometry were used to detect the content of nitric oxide (NO), activity of superoxide dismutase (SOD) and apoptosis rate.ResultsIn the model group, SBP, cardiac indexes and myocardial apoptosis rate increased, the content of NO and activity of SOD decreased compared with the control group. While, in the L-Arg treating group, SBP, cardiac indexes and apoptosis rate decreased, the content of NO and the activity of SOD increased compared with the model group.ConclusionMyocardial apoptosis may play an important role in the pathogenesis of myocardial hypertrophy and cause the losing of myocardial cells. L-Arg induces the increasing production of NO and inhibits myocardial apoptosis through increasing the activity of SOD.

Objective To investigate changes of brainstem auditory evoked potential (BAEP)and somatosensory evoked potential (SEP) in patients with acute cerebral infarction,and discuss their relation with prognosis of the patients.Methods The study involved 60 patients with acute cerebral infarction.Changes of BAEP and SEP in each patient were detected and recorded continuously.Prognosis evaluation was performed by using GCS.Another 60 age-matched and gender-matched healthy human beings were enrolled as controls.Results Incubation period of BAEP wave Ⅰ had no significant difference between the cerebral infarction and control groups (P ＞ 0.05).However,interspike intervals of other BAEP waves in cerebral infarction group were different from those in control group (P ＜ 0.05).A series of waves of SEP (P14-N60) were all significantly prolonged in cerebral infarction group (P ＜0.05).Conclusion BAEP and SEP can effectively reflect function of brain stem in patients with acute cerebral infarction and have some values in determining their prognosis.

In this study, we explored the feasibility of biotin-mediated modified polymeric micelles for pancreatic cancer targeted photodynamic therapy. Poly (ethylene glycol)-distearoyl phosphatidyl ethanolamine (mPEG2000-DSPE) served as the drug-loaded material, biotin-poly(ethylene glycol)-distearoyl phosphatidyl ethanolamine (Biotin-PEG3400-DSPE) as the functional material and the polymeric micelles were prepared by a thin-film hydration method. The targeting capability of micelles was investigated by cell uptake assay in vitro and fluorescence imaging in vivo and the amounts of Biotin-PEG-DSPE were optimized accordingly. Hypocrellin B (HB), a novel photosensitizer was then encapsulated in biotinylated polymeric micelles and the anti-tumor efficacy was evaluated systemically in vitro and in vivo. The results showed that micelles with 5 mol % Biotin-PEG-DSPE demonstrated the best targeting capability than those with 20 mol % or 0.5 mol % of corresponding materials. This formulation has a small particle size [mean diameter of (36.74 ± 2.16) nm] with a homogeneous distribution and high encapsulation efficiency (80.06 ± 0.19) %. The following pharmacodynamics assays showed that the biotinylated micelles significantly enhanced the cytotoxicity of HB against tumor cells in vitro and inhibited tumor growth in vivo, suggesting a promising potential of this formulation for treatment of pancreatic cancer, especially those poorly permeable, or insensitive to radiotherapy and chemotherapy.

Objective To investigate the effect of Fukeqianjin tablets combined with progesterone on serum immunoglobulin,serum IL-2 and soluble receptor levels in patients with functional uterine bleeding,and to explore the optimal regimen for patients with functional uterine bleeding.Methods A total of 98 patients with functional uterine bleeding diagnosed in our hospital from June,2015 to June,2016 were randomLy divided into observation group and control group(n=49).The patients in the control group were treated with oral progesterone and the observation group.The control group was treated on the basis of the combination of gynecological Qianjin Tablet,compared the two groups of patients before and after treatment of menstrual conditions,serum IgM,IgA,serum IL-2 and soluble receptor levels.Results In the first month,the second month and the third month after treatment,the menstrual volume in the observation group were(103.5±21.5)mL,(93.5±14.7)mL,(81.4± 12.2)(7.68±0.92)d,(6.81±0.87)d and(6.14±0.78)d respectively in the observation group were significantly shorter than those in the control group(P<0.05).After treatment,the menstrual period The serum levels of IgA and IgM were(3.6±0.8),(2.6±0.8),(1.8±0.3)and(2.9±0.5),(2.2±0.3),(1.1±0.2)in the first month,the second month and the third month respectively,Significantly lower than the control group,the difference was statistically significant; the first month after treatment,the first two months,the first three months,observation group of patients with serum IL-2 and soluble receptor levels significantly Lower than the control group.Conclusion Fukeqianjin tablets combined with progesterone can improve the immune function of patients with functional uterine bleeding,reduce the level of inflammation and soluble receptor levels,compared with single progesterone treatment better,the program better.

Objective To investigate the characteristics of 136 patients with occupational diseases,to summarize key techniques used in field investigation,and to provide a scientific basis for the development of standard operating procedures for field investigation of occupational disease diagnosis.Methods Field investigation and routine data analysis were performed to analyze the cases diagnosed by Guangdong Provincial Hospital for Occupational Disease Prevention and Treatment from January 2009 to December 2014.Results A total of 136 cases of occupational diseases were diagnosed by Guangdong Provincial Hospital for Occupational Disease Prevention and Treatment from 2009 to 2014,and there were 66 cases of leukemia,18 cases of suspected occupational benzene poisoning,12 cases of suspected occupational hand-arm vibration disease,and 11 cases of suspected pneumoconiosis.Of all these patients,41.91％ were engaged in at least three types of work,70.59％ were exposed to at least three types of chemicals,25.74％ experienced changes in technical processes and chemicals,and 47.06％ had disputes on the chemicals they were exposed to during verification by both parties.Occupational hazard factors were detected.Most samples (358) were used to measure benzene concentration in workplace air,among which 11.7％ had a benzene concentration of ＞6.00 mg/m3 (exceeding standard),13.41％ had a benzene concentration of 3.26～6.00 mg/m3,75.42％ had a benzene concentration of＜ 0.03 to ＜3.25 mg/m3.The samples of suspected occupational hand-arm vibration disease,suspected pneumoconiosis,and suspected occupational noise-induced hearing loss had high over-standard rates (100％,93.8％,and 83.3％,respectively).Conclusion Field investigation of occupational disease diagnosis reveals large numbers of cases of leukemia,suspected occupational benzene poisoning,suspected occupational handarm vibration disease,and suspected pneumoconiosis.The key aspects of field investigation include confirmation of the history of occupational exposure,identification of occupational hazard factors,confirmation of the changes in technical processes and chemicals,detection of occupational hazard factors,sampling and analysis of raw materials,and epidemiological investigation of workers with the same type of work.

Objective To investigate the characteristics of 136 patients with occupational diseases,to summarize key techniques used in field investigation,and to provide a scientific basis for the development of standard operating procedures for field investigation of occupational disease diagnosis.Methods Field investigation and routine data analysis were performed to analyze the cases diagnosed by Guangdong Provincial Hospital for Occupational Disease Prevention and Treatment from January 2009 to December 2014.Results A total of 136 cases of occupational diseases were diagnosed by Guangdong Provincial Hospital for Occupational Disease Prevention and Treatment from 2009 to 2014,and there were 66 cases of leukemia,18 cases of suspected occupational benzene poisoning,12 cases of suspected occupational hand-arm vibration disease,and 11 cases of suspected pneumoconiosis.Of all these patients,41.91％ were engaged in at least three types of work,70.59％ were exposed to at least three types of chemicals,25.74％ experienced changes in technical processes and chemicals,and 47.06％ had disputes on the chemicals they were exposed to during verification by both parties.Occupational hazard factors were detected.Most samples (358) were used to measure benzene concentration in workplace air,among which 11.7％ had a benzene concentration of ＞6.00 mg/m3 (exceeding standard),13.41％ had a benzene concentration of 3.26～6.00 mg/m3,75.42％ had a benzene concentration of＜ 0.03 to ＜3.25 mg/m3.The samples of suspected occupational hand-arm vibration disease,suspected pneumoconiosis,and suspected occupational noise-induced hearing loss had high over-standard rates (100％,93.8％,and 83.3％,respectively).Conclusion Field investigation of occupational disease diagnosis reveals large numbers of cases of leukemia,suspected occupational benzene poisoning,suspected occupational handarm vibration disease,and suspected pneumoconiosis.The key aspects of field investigation include confirmation of the history of occupational exposure,identification of occupational hazard factors,confirmation of the changes in technical processes and chemicals,detection of occupational hazard factors,sampling and analysis of raw materials,and epidemiological investigation of workers with the same type of work.

Objective To explore the effect of preventive management path of breast cancer related lymphedema based on risk reduction program on breast cancer patients underwent axillary lymph nodes dissection (ALND). Methods This study used convenient sampling method to recruit 60 patients underwent ALND. Patients were randomly divided into the control group (n=31) and the intervention group (n=29). All patients received general care while patients of the intervention group also received the Risk Reduction Program. Lymphedema symptom experience was investigated by Breast Cancer and Lymphedema Symptom Experience Index 1 month, 3 months, 6 months, 12 months after surgery. Results Twelve months after surgery, the intervention group had 4.00(8.00)lymphedema related symptoms while the controll group had 9.00(7.00), the difference was significant (Z=-2.023, P=0.043). The score of lymphedema related symptom distress in the intervention group was 3.00(7.00)while it was 7.00(17.00)in the control group, the difference was significant (Z=-2.159, P=0.031). The scores of functional dimension and sexual dimension in the intervention group were 0(2.00)and 0(0), and in the control group were 3.00(5.00)and 0(1.00), the differences were significant (Z=-2.315,-2.334, P=0.021, 0.020). No significant differences of lymphedema rate existed between groups (P>0.05). Conclusion The preventive management path of breast cancer related lymphedema based on risk reduction program can decrease the number of lymphedema symptoms and release lymphedema symptom distress.

Objective To explore the application effect of quality control circle in the quality management improvement of the occupational disease diagnostic and code.Methods To established across departmental team of medical record QCC,we Select 132 cases of the occupational disease departmentin specialized Hospital from May 1,2022 to June31,2022,Analyze the reasons of inaccurate that main diagnosisselection and code mapping.After formulating a series of improvement measures,we Se-lect 71 cases of occupational disease department from November 1,2022 to December 31,2022.To compared effect that before and after the implementation of QCC.Results After6 months of improvement,The utilization rate of main diagnosis mapping Z-code was decreased from 36.3%to 12.7%,goal achievement rate of 106.3%,improvement rate of 65.0%.Enrollment rate of major diagnostic was increased from 53.0%to 86.3%,goal achievement rate of 116.4%,Improvement rate of 62.8%.Conclu-sion We have changed the quality control management mode of diagnosis and coding by the QCC,implement targeted feedback,supervision,and training,establish a reward and punishment mechanism that matches indicators to improved accuracy of enroll-ment.Upgrading the quality management level of medical records and medical safety at the same time.Should continue to pro-mote theimplementation.

Infectious stones are produced by urease producing microorganisms, which have a fast generation rate, high recurrence and mortality rates, and are prone to complications related to infection. At present, the treatment of infectious stones includes surgical treatment and drug treatment, and the research on its treatment methods has become one of the hotspots in the field of urology. This article provides a review of the research progress in the treatment of infectious stones, with the aim of improving understanding of the treatment of infectious stones.

Objective:To explore effect and feasibility of family members' encouragement video on improving the psychological state of patients in declining visiting Cardiac Surgical Intensive Care Unit (ICU) .Methods:A total of 300 patients who were hospitalized in Cardiac Surgical ICU of Beijing Chaoyang Hospital Affiliated to Capital Medical University from January 1 to December 31, 2019 were selected. The patients were divided into the experimental group ( n=150) and the control group ( n=150) by the random number table method. The patients in the experimental group adopted the intervention method of watching encouragement videos of family members, while patients in the control group adopted routine care (that was, the nurses encouraged the patients after telephone communication with their family members) . The psychological status of patients in the two groups before and after the intervention was evaluated using Mental Status Scale in Non-psychiatric (MSSNS) , and the differences in the scores between and within the two groups before and after the intervention were compared. Results:After the intervention, the MSSNS total score, depression score and loneliness score of the experimental group showed a downward trend, and the differences were statistically significant compared with those before the intervention ( P<0.05) . There was no statistically significant difference in all aspects of the control group before and after the intervention ( P>0.05) . After the intervention, the MSSNS total score and loneliness score of the experimental group were lower than those of the control group, and the differences were statistically significant ( P<0.05) . Conclusions:Encouragement video of family members can improve the psychological state of patients in declining visiting Cardiac Surgical ICU, mainly to relieve the negative emotions related to loneliness and depression.