OBJECTIVE:To provide references for the enhancement drug quality management level.METHODS:Through analyzing the demands on drug quality monitoring information and by using Visual Studio.NET,the software of the browser/server model information system for drug quality monitoring was designed.RESULTS & CONCLUSIONS:The newly developed browser/server model information system for drug quality monitoring has many advantages,and it can better satisfy the requirements for drug quality management.

Objective To observe the changes of cognition ability of the middle cerebral artery occlusion(MCAO) rats,following varied lengths of cerebral ischemia-reperfusion.Methods Totally 36 SD rats(female or male) were randomly and equally divided into 3 groups,the sham group,the ischemia 30 min group and the ischemia 90 min group.The model of MCAO was made by occluding the MCA with thread according to (Longa's) method.Behavioral assessment was evaluated by neurological test.Brain tissue damage was detected in the ischemia animals 14 d after the surgery by triphenyl tetrazolium chloride(TTC) staining.Cognition ability was determined by Morris-maze test and P300 test.Results The score of neurological test were both over 2 points of ischemia groups of 30 min and 90 min.And both groups had infarct areas by TTC staining.The results of Morris-maze test and P300 test showed that the ischemia 90 min group were improved significantly in comparison with sham group and the ischemia 30 min group(P

BACKGROUND:Methylene blue can hinder abnormal pain conduction via the sensory nerve, and its mechanism is to block bradykinin-induced hyperalgesia and eliminate pain caused by local tissue inflammation.
OBJECTIVE:To observe the influence of methylene blue solution on the lumbar spinal cord and spinal ganglia function of rats, and to investigate whether methylene blue is safe for treating discogenic low back pain.
METHODTotal y 120 Wistar rats were randomly divided into five groupthree experimental groups, a saline control group and a blank control group, n=24 in each group. Lumbar segmental dura was exposed in rats. In the three experimental groups, 0.2, 1, and 2 mL methylene blue were injected epidural y, respectively. The saline control group was subjected to the epidural injection of 1 mL saline. In the blank control group, there was no treatment. Six rats from each group were selected randomly and perfused at 30 minutes, 2 hours, 24 hours, 72 hours after injection, respectively. Then, the corresponding segments of the spinal cord and ganglions were removed. Hematoxylin eosin staining was used for comparing histological and structural changes under light microscope.
RESULTS AND CONCLUSION:Hematoxylin-eosin staining showed that at 30 minutes, 2 hours, 24 hours and 72 hours after injection of methylene blue, the spinal dorsal side exhibited the structural integrity, clear boundaries between the white matter and gray matter, dense nerve fibers in the white matter, and round or oval nuclei of glial cells among fibers;dense nerve fibers in the posterior horn of gray matter;light-colored neuronal nuclei with prominent nucleoli;a bundle of nerve fibers among cellpopulations. There was no significant change in tissue structure of lumbar spinal cord and spinal ganglia between the experimental groups and the saline control group or between the experimental groups and the blank control group. Thus, the epidural injection of 1%methylene blue has no significant effect on the spinal cord and spinal nerve structures.

BACKGROUND: Tendon is a special connective tissue that can transmit the force generated by the muscle to the bone through the enthesis. The occurrence of tendinopathy is closely related to the mechanical characteristics of tendon and enthesis and its micro-structure, but the specific mechanism still remains controversial OBJECTIVE: To summarize the micro-structure of tendon and en thesis, the biomechanical characteristics of tendon, the healing and repair of tendon injuries, and the biomechanical factors related to chronic tendinopathy. METHODS: CNKI, VIP, CBM, WanFang, PubMed and Embase databases were searched. The articles concerning micro-structure of tendon and enthesis, relationship between tendinopathy/enthesis and stress, and biomechanical mechanisms of tendinopathy/enthesis were selected. RESULTS AND CONCLUSION: The biomechanical properties of tendon and enthesis are closely related to their microstructures. The biomechanical properties of tendon are reduced due to the damage of their microstructures in the process of repair. The occurrence and development of chronic tendinopathy are related to the microstructures and biomechanical characteristics of enthesis. Compression load and stress shielding of enthesis may play a key role, but further high-quality studies on the specific mechanism are still required.

BACKGROUND: Tendinopathy is a hotspot in the fields of sports medicine and rehabilitation, but there have been many controversies about the concept, mechanism and pathological stages of tendinopathy for a long. OBJECTIVE: To systematically review and summarize the related concepts of tendinopathy, the role of inflammation in the occurrence of tendinopathy and the pathological mechanism of tendinopathy. METHODS: CNKI, VIP, CBM, WanFang, PubMed and Embase databases were searched. From the aspects of tendinopathy, enthesis, inflammation and pathological mechanism, some related research results since 1990 were retrieved and summarized. RESULTS AND CONCLUSION: The results show that the debate on the concept of tendinopathy is based on the understanding of its pathological mechanism, and the understanding of the pathological mechanism of tendinopathy will continue to be improved with the emergence of more high-quality researches. The research on the relationship between tendinopathy and inflammation, the relationship between tendinopathy and load patterns, and the mechanical characteristics of enthesis will be a future focus.

BACKGROUND:It is controversial whether anterior approach alone, or combined anterior and posterior approaches were used for high level and multiple segments of severe ossification of cervical posterior longitudinal ligament.
OBJECTIVE:To explore the difference of anterior approach versus combined anterior and posterior approaches for the treatment of high level and multiple segments of severe ossification of cervical posterior longitudinal ligament.
METHODS:A total of 21 cases of high level and multiple segments of severe ossification of cervical posterior longitudinal ligament were included in this study. There were 9 males, aged 56-72 years, and 12 females, aged 58-70 years. We used anterior decompression and titanium mesh bone graft fusion in 11 cases which lesion located between C2-5 vertebra, and ossification excision, combined anterior (titanium mesh plate and screw) and posterior (lateral mass screw) approaches in 10 cases which between C3-7 vertebra. Japanese Orthopaedic Association score system was used to evaluate the results. The excellent and good rate and improvement rate were calculated.
RESULTS AND CONCLUSION:The excellent and good rate was 90%and improvement rate was 82%in 10 cases using combined anterior and posterior approaches. The excellent and good rate was 73%and improvement rate was 73%in 11 cases using anterior treatment alone. Significant differences in the excellent and good rate and improvement rate were detected between the two groups (P<0.05). These suggested that combined anterior and posterior approaches for high level and multiple segments of severe ossification of cervical posterior longitudinal ligament is a better operative procedure.

Objective To study the expression levels of serum HSP70 and p53 in gastric cancer and its cor-relation with helicobacter pylori infection.Methods 50 patients with gastric cancer were selected as the research object.Among 50 cases,30 cases were stage Ⅰ -Ⅱ,20 cases were stage Ⅲ,22 cases were with lymph node metasta-sis and 28 cases were without lymph node metastasis.40 healthy subjects were selected as the healthy control group. The serum levels of HSP70 and p53 were detected.Results In the gastric cancer group,the serum levels of HSP70 and p53 were markedly higher than those of the healthy control group,and the difference was statistically significant (t =12.53,16.79,all P <0.01).Moreover,with progressing of clinical stage of gastric cancer,the serum levels of HSP70 and p53 showed a trend of increasing(t =4.68,5.29,all P <0.01).The serum expression levels of HSP70 and p53 in gastric cancer with lymph node metastasis were significantly higher than those of patients without lymph node metastasis and the difference was still statistically significant(t =3.82,4.39,all P <0.01).The serum levels of HSP70 and p53 in the gastric cancer group with Hp positive were much higher,compared to those of cases with Hp negative and the difference was also statistically significant(t =4.72,4.17,all P <0.01).Conclusion In the gastric cancer patients,the serum expression level of HSP70 and p53 are increased.The expression of HSP70 and p53 are closely related to the progress and prognosis of gastric cancer and Hp infection.

BACKGROUND:In recent years, research on tendinopathy is becoming a hot topic in the sports medicine and rehabilitation, and the extracelular matrix plays a considerable role in the occurrence, development and healing of tendinopathy.
OBJECTIVE: To explore the relationship between tendinopathy and the extracelular matrix.
METHODS: CNKI, VIP, CBM, Wanfang, PubMed and Embase were retrieved for articles related to colagen, protein, glycoprotein and matrix remodeling published since 2000.
RESULTS AND CONCLUSION:After tendinopathy, there are obvious changes in colagen, proteoglycans, glycoprotein and related enzymes; and these changes also affect the reconstruction and repair of tendinopathy. Currently, most of researches focus on the tendon rather than the bone-tendon junction. This means that there are no in-depth studies on the correlation between many important proteins and tendinopathy, and the effects of exercise intervention.

Objective To learn the size and income of off-staff medical workers in a survey of public county-level general hospitals in China.Methods Human resources investigation of public county-level general hospitals,with SPSS data analysis of the outcomes.Results From 2011 to 2013,the proportion of off-staff medical workers increased from 31.44% to 36.69%,of whom the highest proportion was found in China′s western region,accounting for 40.69%;the ratio of off-staff practicing physicians increased from 14.24% to 16.64%,of whom China′s central region accounted for 19.92%;the ratio of off-staff nurses increased from 43.03% to 49.77%,of whom China′s western region accounted for 55.88%.In 13.7% of the hospitals,income of off-staff medical workers was less than a half of that for permanent staff;41.32% and 26.31% of the hospitals surveyed were fully covered with social insurance and housing fund account respectively.Conclusions Off-staff labor is a distinct feature at such hospitals,making imperative a HR system reform of public hospitals.The discriminative income and welfare between permanent and off-staff medical workers calls for a fundamental change to the income and welfare system.

OBJECTIVE:To investigate the application structure and tendency of eye drops for external use so as to provide references for the research & development,production and use of this preparation.METHODS:Data including variety,classification,consumption amount and sales volume of the eye drops from 2004 to 2006 in the outpatient were analyzed retrospectively by hospital information system.RESULTS & CONCLUSION:The application structure of eye drops in our hospital was basically stable,but control on the use of anti-infective drugs needs to be tightened;the amount of anti-xeroma and anti-asthenopia drugs increased to a large extent;the imported variety takes an excessively large proportion.The use of anti-infective drugs should be standardized and the market share of domestic drugs remains to be further enlarged.