Objective To explore the application value of color Doppler ultrasound in screening of fetal con-genital heart disease and its significance.Methods 4 132 cases of the expectant mothers were selected,using color Doppler ultrasonography on the line screening of five section to detect fetal cardiac,to compare with the actual clinical situation and analyze the occurrence of fetal congenital heart disease.Results Among 50 cases(1.21%) of congeni-tal heart disease,28 cases were live births, accounted for 56.00%,13 cases of induced abortion, accounted for 26.00%,9 cases died,accounted for 18.00%.48 cases were detected by ultrasound,relevance ratio was 1.16%,the accuracy rate was 96.00%,the missed diagnosis rate was 4.00%.28 cases of live births undergoing surgical correc-tion,treatment effect was good,48 cases were detected,including 10 cases of ventricular septal defect,6 cases of tetral-ogy of Fallot,6 cases of atrial septal defect,5 cases of endocardial cushion defect,4 cases of single chamber,4 cases of persistent truncus arteriosus,3 cases right ventricular dysplasia,3 cases of tricuspid valve shift malformation,2 cases of persistent left superior vena cava,2 cases left ventricular dysplasia,2 cases of aortic arch interruption,1 case of rhabdomyoma.Conclusion Using color Doppler ultrasound detection for pregnant women can effectively detect severe congenital heart disease in the fetus,stop gravid timely,which can effectively improve the level of prenatal and postnatal care that is worth clinical application.

Objective Adenoid cystic carcinoma (ACC), a rare malignancy in head and neck region, is predominately found in the salivary glands. Our study is to retrospectively analyze the treatment outcomes and prognostic factors of ACC originated from the major salivary glands. Methods A total of 54 patients diagnosed as ACC were treated in our institution, including 24 cases originated from the parotid gland and 30 from the submandibular or sublingual gland. According to the records, 26 patients received surgery alone and 28 were treated with surgery followed by radiotherapy with a median dose of 58 Gy (range, 50 -65 Gy). Results The Follow-up rate was 94%, and 15 patients from postoperative radiotherapy group and 20 from surgery alone group were followed up more then 5 years. The 5-year overall survival rate, local-regional control rate, distant metastasis rate, and disease-free survival rate were 97%, 71%, 13% and 69%, respectively. Lung metastasis, occurred in 7 patients, was the most common distant failure. Fifteen recurrences were observed, including 13 in surgery alone group and 2 in postoperative radiotherapy group. The 5-year local-regional control and disease-free survival rates were 90% and 85% for patients treated with postoperative radiotherapy, 54% and 55% for those treated with surgery alone. Univariate and multivariate analyses showed that postoperative radiotherapy was the only prognostic factor of local-regional control and survival rates. Other parameters such as nerve involvement did not significantly influence the treatment results. Conclusions Postoperative radiotherapy can improve the prognosis of ACC originate from the major salivary glands compared with surgery alone. Distant metastasis is an obstacle in curing the disease, which indicates the value of systemic treatment.

Objective To observe the clinical effect of calcitonin(PCT) in the identification of bacterial infection and viral infection.Methods 150 cases were randomly selected,including 50 cases of viral infection,100 cases of bacterial infection.The people with physical examination at the same period in the hospital whose peripheral blood culture were negative were selected as control group.PCT concentrations of various bacterial infections were observed,as well as the concentrations of PCT in various viral infections,and compared with the control group.Results The PCT level in the bacterial infection group was (8.023±3.542)ng/mL, which was significantly higher than (0.031±0.011)ng/mL in the control group(t=15.960,P=0.000).There was no significant difference in the level of PCT between the viral infection group [(0.032±0.008)ng/mL] and the control group [(0.031±0.011) ng/mL] (t=1.903,P=0.059).In different bacterial infections,the PCT level in sepsis was the highest [(17.544±4.511)ng/mL],followed by bacterial peritonitis and infectious pancreatitis[(5.131±1.231)ng/mL,(4.941±1.665)ng/mL].The PCT level of cellulitis was the lowest [(27.544±4.511)ng/mL].Of a variety of viral infections,the patients with EB virus infection had slightly higher PCT level[(0.027±0.002)ng/mL],which of measles virus infection was slightly lower[(0.021±0.002)ng/mL],the overall PCT level had no significant difference.Conclusion It is important to identify the bacterial infection and viral infection, which can provide a reliable basis for clinical treatment.

Objective To investigate the structure of the visiting technicians in Physiotherapy Department of Beijing Bo'ai Hospital, China Rehabilitation Center in the past 8 years. Methods 923 technicians visiting Physiotherapy Department from 2007 to 2014 were classified in the terms of provinces from, genders, ages, academic degrees, registers of practices and levels of hospital. Results Technicians visiting Physiotherapy Department came from 28 provinces (municipalities or municipality cities), (115.38 ± 5.30) persons per year, mainly aged 25-34 years (60.78%). The majority of the technicians (51.14%) accepted junior college education, and 46.37% did not registered for rehabilitation related practices. The visiting technicians were more and more year by year, especially rehabilitation technicians. Genders, ages and academic degrees influenced on the proportion of rehabilitation technicians. Conclusion Institutes sending the visiting technician need to balance the genders, ages and registers of practice, to meet the good practice.

To introduce a rehabilitation program for total hip replacement (THR), including muscle strength training; rehabilitation for scar and adhesion, scoliosis, hip control, etc. The principle and the cautions were discussed.

Background and purpose:Studies have shown that some molecular markers could serve as prognostic factors for nasopharynx carcinoma, but the predictive role of catenins and cyclin D1 remains uncertain for the disease. Our paper is to investigate the expression of catenins(?-,?- and ?-) and cyclin D1 in nasopharyngeal carcinoma as well as to analysis their relation to clinic factors and prognosis. Methods:We retrieved 38 paraffin-embedded specimens of nasopharynx carcinoma, immunohistochemistry was used to examine the expression of ?-,?- and ?-catenin , cyclin D1 and tumor proliferation activity marker ki-67.Results:Reduced expression of ?-,?- ,?-catenin and cyclin D1 was observed in most of the tumors. Our preliminary study demonstrated that there was no significant correlation between their expression with T-stage, N-stage, clinical stage and primary tumor volume, as well as with ki-67 stain. In unviarance analysis, patients with reduced expression of ?-catenin had poorer prognosis than those with high expression, 5 year overall survival and disease free survival rates of these two groups were 53.2%, 29.0% and 81.9%, 76.0%, respectively(P

60 cm 3). Primary tumor volume was found to be an independent prognostic factor of local control in multivariant analysis without any statistical significance to predict the disease-free survival or distant relapse-free survival rates. Conclusion The greater volume disparity with the same T stage and the data extension overlap with different T stages are demonstrated and the primary tumor volume may be considered as a prognostic factor in the treatment of nasopharyngeal carcinoma.

Objective To investigate the attention and awareness of risk management in physical therapists during their treatments. Methods The staffs visited China Rehabilitation Research Center for studying physical therapy from other hospitals were investigated with a questionnaire. Results and Conclusion 41 out of 52 questionnaires came back. The consciousnesses of risk management were variable with the profession they engaged before, that the physicians were the best.

Objective To characterize the function of human anti-BP180 single-chain Fv antibody (scFv) in vitro. Methods The IgG autoantibodies against BP180 were purified by affinity chromatography from the sera of patients with BP. The inhibitive effect of previously constructed anti-BP180 scFv on the binding of anti-BP180 IgG autoantibodies to the recombinant NC16A domain of human BP180 antigen was observed by competitive ELISA, competitive immunofluorescence assay and competitive inhibition test for complement activation. Results As ELISA revealed, the scFv significantly inhibited the binding of anti-BP180 IgG autoantibodies to the corresponding antigen (P ＜ 0.01 ), and the inhibitive effect was dose-dependent within the concentration range from 0 to 60 μg/ml. The inhibitive rate peaked at 69.50%. The deposition of anti-BP180-IgG autoantibodies in basement membrane zone and the IgG autoantibody-mediated complement C3 activation were both suppressed by the scFv of 40 μg/ml. Conclusion The genetically engineered anti-BP180 scFv has a certain inhibitive effect on the binding of BP-IgG autoantibodies to BP180 antigen and on the subsequent complement activation in vitro.

Background and purpose:Radiotherapy is the main method to manage head and neck tumors.About half of the patients with malignant tumor in head and neck could obtain long term survival through radiotherapy or other therapy combined with radiotherapy.However,it is necessary to clinically detect whether carotid stenosis is a major sequela of head and neck irradiation,especially in long term survivors such as patients with nasopharyngeal carcinoma.We analyzed this injury and the degree of carotid stenosis,and its related factors to provide information to reduce and delay the incidence of this injury.Methods:We analyzed the degree of arterial stenosis of the nasopharyngeal carcinoma patients who received radiotherapy and had survived more than three years in Cancer Hospital Fudan University,compared with newly diagnosed as nasopharyngeal carcinoma before radiotherapy.The degree of carotid artery injury was observed and the clinical factors which affect its occurrence and development.All patients were low differentiated squamous cell carcinoma confirmed by pathology.All patients were examined with color doppler ultrasound to detect the carotid artery.The main end points were the diameter of carotid artery,the peak systolic and end diastolic velocities.Results:Arterial stenosis was more common in the reserch group than the control group(80% vs 20%,P＜0.001).The common and internal carotid arteries were most commonly involved(70% vs 20%,P＜0.001;6313% vs 10%,P＜0.001),followed by the external carotid artery(30% vs 3.3%,P=0.015).Significant stenosis was only found in the research group,common carotid arteries 36.7%,internal carotid arteries 23.3%,external carotid arteries10%.Conclusion:This study showed that radiation could cause significant carotid stenosis in nasopharyngeal carcinoma patients who had received radiotherapy more than 3 years previously.Carotid stenosis developed more frequently in the common carotid artery and intemal carotid artery on the side that had received radiotherapy.