There have been problems in the existing multiple physiological parameter real-time monitoring system, such as insufficient server capacity for physiological data storage and analysis so that data consistency can not be guaranteed, poor performance in real-time, and other issues caused by the growing scale of data. We therefore pro posed a new solution which was with multiple physiological parameters and could calculate clustered background data storage and processing based on cloud computing. Through our studies, a batch processing for longitudinal analysis of patients' historical data was introduced. The process included the resource virtualization of IaaS layer for cloud platform, the construction of real-time computing platform of PaaS layer, the reception and analysis of data stream of SaaS layer, and the bottleneck problem of multi-parameter data transmission, etc. The results were to achieve in real-time physiological information transmission, storage and analysis of a large amount of data. The simulation test results showed that the remote multiple physiological parameter monitoring system based on cloud platform had obvious advantages in processing time and load balancing over the traditional server model. This architecture solved the problems including long turnaround time, poor performance of real-time analysis, lack of extensibility and other issues, which exist in the traditional remote medical services. Technical support was provided in order to facilitate a "wearable wireless sensor plus mobile wireless transmission plus cloud computing service" mode moving towards home health monitoring for multiple physiological parameter wireless monitoring.
Computer Systems ; Computers ; Humans ; Information Storage and Retrieval ; methods ; Internet ; Monitoring, Physiologic ; methods ; Software

Objectives To determine the predictive effect of the transvaginal ultrasonographic cervical measurement in induction of labor among the nulliparous. Methods This prospective observational study recruited women with singleton gestation scheduled for induction of labor at ≥37 weeks. All the pregnancy women were nulliparous. Transvaginal ultrasonographic cervical measurement was performed and Bishop score was detemined, each by operators masked to the other measurement. Data were collected on parity, gestational age, mode of delivery, induction agent, induction onset of labor interval, Bishop score and cervical measurement. Results A total 121 women were included. A stepwise regression model identified cervical length was the best linear predictor of induction to onset of labor interval. ( r=0.67, P

Objective To study the inhibitory effect of radicamine A on ?-glucosidase and the glucose's absorption by small intestine.Methods Inhibitory effect of radicamine A was studied both in ?-glucosidase inhibition experiments and by a valgus cyst model of small in testine in vitro.Results Radicamine A inhibited ?-glucosidase and glucose's absorption in a dose-dependent manner(P

Objective To explore the use of urine LH test paper associating basal body temperature in ovulation monitoring and conception guiding in anovulatory infertility.Methods We used clomiphene to treat 100 anovulatory infertility patients.Then we recorded basal body temperature and used urine LH test paper in morning to determine whethere there is urine LH peak between the 12th to 20th day of menstrual cycle.If the patient become pregnant,we stop observation.If not,we observed them until the third treatment cycle completed.Result Before treatment,both the urine LH test paper and basal body temperature showed there was no ovulation.After treatmend,we observed 199 menstrual cycle.There were urine LH peak in 149 menstrual cycle.And there were double phase body temperature in 158 treat cycle.The lowest body temperature appeared in the urine LH peak day or after it.71.1%of the lowest body temperature emerged in one or two days after the urine LH peak appeared.76 patients in our study become pregnant,pregnancy rate is 76%.Conclusion Urine LH test paper associating basal body temperature is convenient and easy tO learn.It is a good way in ovulation monitoring and conception guiding.

Objective To evaluate the clinical effects of Qufeng Jiedu Huoluo Decoction in treating the acute stage of periph-eral facial paralysis.Methods 60 cases of acute stage of peripheral facial paralysis were randomly divided into 2 groups,30 cases in each group.The two groups were treated by acupuncture.In addition the treatment group was added with oral Qufeng Jiedu Huoluo Decoction.Results The relief time of posterior auricular pain in the treatment group was shorter than that in the control group (P <0.05);the alleviation situation of the accompanying symptoms after 7 d and the neural function recovery situation after 14 d in the treatment group were superior to those in the control group (P < 0.05);the total effective rate in the treatment group was 93.3%,which was higher than 83.4% in the control group,the difference had statistical significance(P < 0.05).Conclusion Qufeng Jiedu Huoluo Decoction can effectively alleviate the clinical symptoms and signs of acute stage in peripheral facial paralysis.

Aim This study aimed to assess the protection of recombinant human erythropoietin (rhEPO) in light-induced injuries in human retinal pigment epithelial(RPE)cells by researching the inhibition of rhEPO for apoptosis in human RPE cells by light-induced injuries.Methods Cultured human RPE cells were exposed to light of 8 w (2 000?500) lux for 12hours,then the culture were stopped at 24 hours after 12hours light stimulation. The effect of inhibiting apoptosis of rhEPO was detected by AnnexinV-flunorescein isothiocyanate/Propidium iodium labeling and flow cytometry. The enzyme linked immunosorbant assay(ELISA)and immunocytochemical staining were used to assess the expressions of caspase-3 and Bcl-2 treated by different doses of rhEPO in light-induced injury on human RPE cells and research the protective mechanism of rhEPO by adding AG490(the special inhibitor of Jak2).Results There was a obviously increased effects on inhibiting apoptosis in every rhEPO group, which was the most conspicuous in 40 IU?ml-1 rhEPO group,and the value was (4.93?1.45)?ml-1. The decrease of expression of caspase-3 was most obvious in 40 IU?ml-1 rhEPO group, and the value was (0.125?0.029) ?g?L-1. The increase of expression of Bcl-2 was the most obvious in 40 IU?ml-1 rhEPO group and the value was 168.21?3.87. But these effects on inhibiting apoptosis in rhEPO group were restrained by adding AG490, the value of apoptosis was (11.29?2.11)?ml-1 and the density of caspase-3 increased to (0.362?0.042) ?g?L-1,the expression of Bcl-2 dropped.Conclusion It is suggested that rhEPO can inhibit the apoptosis of human RPE cells in the light-induced injuries and inhibit the expression of caspase-3 and up-regulate the expression of Bcl-2, so rhEPO can protect the light-induced injuries for human RPE cells. Its protective mechanism is accomplished principally by the pathway of combining EPO with EPOR ,then the combination activates Jak2.

Objective To explore the sexual life quality of females at the reproductive ages after cervix cancer operation and supply evidence for establishment of correspondindg nursing measure. Methods We investigated the frequency of sexual life and satisfaction degree of sexual life in 40 females at the reproductive ages after cervix cancer operation by face-to-face investigations using questionnaires before and 4 months after operations. Then the difference of sexual life quality before and after operation were compared and analyzed. Results The frequency of sexual life (P＜0.01,P＜0.05) and satisfaction degree of sexual life (P＜0.01,P＜0.05) were evidently decreased. The sexual life quality was lower than that before operation. Conclusion The sexual life quality could be affected by many factors such as economic conditions, culture background, sexual conceptions and the attitude of their partners.

BACKGROUND: Basic fibroblast growth factor (bFGF), a kind of polypeptide growth factor possessing multifunctional biological activities,can protect neurons and promote the growth of nerves. It has been corfirmed that bFGF has therapeutic effects on retina ischemia/reperfusion injury (RIRI).OBJECTIVE: To establish RIRI model and analyze the effects of bFGF on cellular apoptosis of retina and the expression of regulatory gene protein.DESIGN: Randomized grouping and validating trial.SETTING: Department of Ophthalmology, the Affiliated Hospital of Medical College of Qingdao University.MATERIALS: The experiment was conducted at the Research Laboratory of Pathology, Department of Ophthalmology, Medical College of Qingdao University, from April 2002 to December 2003. Twenty-eight healthy Wistar rats were enrolled in this experiment. Four rats were randomly chosen for normal control group, the left eyes of the other 24 rats were set as normal saline control group, and the right eyes were set as bFGF group.METHODS: Normal saline control group and bFGF group adopted the rat RIRI models established by transiently elevating intraocular pressure. Normal saline of 12 μL was injected into the vitreous cavity of the left eyes of the rats in normal control group. 12 μL bFGF was injected into the vitreous cavity of the right eyes of the rats in bFGF group, 4 rats once. No administration was given in normal control group. The expression of apoptotic cells was detected and apoptosis indexes were calculated with the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labeling (TUNEL) method and immunohistochemical staining method at the 1st, 6th,12th, 24th,48th and 72nd hours after reperfusion and ischemia for 1 hour.MAIN OUTCOME MEASURES: ① The detection results of apoptotic cells in situ of retina tissuesat different time points after reperfusion. ②The expression of Fas and caspases-2 in retina tissues at different time points after reperfusion.RESULTS ① Comparison of apoptosis indexes of retina tissues at different time points after ischemia reperfusion: There were no apoptotic cells in the retina tissues of the rats in normal control group. As compared with those in normal saline control group, apoptosis indexes in bFGF group were significantly decreased at ischemia 1 hour and reperfusion 1, 6, 12, 24, 48and 72 hours, especially at the 12th, 24th and 48th hours after reperfusion (t =5.362-5.595, P ＜ 0.05). ② The change of Fas expression at different time points after ischemia reperfusion: There was hardly any Fas expression in normal control group. As compared with that in normal saline control group, Fas expression in bFGF group was significantlydecreased at ischemia 1 hour and reperfusion 1, 6, 12, 24, 48 and 72 hours, especially at the 6th, 12th and 24th hours after reperfusion (t=3.954-9.327, P ＜ 0.05). ③The changes of caspase-2 expression at different time points after ischemia reperfusion: There was no caspase-2 expression in normal control group.Compared with that in normal saline control group, the number of caspase2 positive cells in bFGF group was significantly decreased at the 6th,12th,24th, 48th and 72nd hours after ischemia for 1 hour and reperfusion (t=4.125-15.641, P ＜ 0.05).CONCLUSION: bFGF can significantly inhibit the expression of apoptosis gene Fas and caspase-2 in the ischemia and reperfusion of retina, thus reducing cellular apoptosis of ganglion cells and exerting therapeutic effects on the ischemia and reperfusion of retina.

Background and purpose: Protein kinase P1k3 could increase the transcriptional activity of p53. However, the effect of P1k3 on the transcriptional activity of p73 is still unknown. Our study was to investigate the effect of P1k3 on the transcriptional activity of p73. Methods: Luciferase reporter assay, RT-PCR and colony formation assay were adopted to study the effect of P1k3 and kinase-deficient P1k3 (K52R) on the transcriptional activity of p73 in p53-deficient human lung carcinoma H1299 cells. Results: Luciferase reporter assay showed that p73 increased the luciferase activities induced by p21~(WAF1) and Bax promoters (P<0.05). After co-transfection with p73 and P1k3, the luciferase activities induced by p21~(WAF1) and Bax promotors were significantly decreased in a dose-dependent manner as compared with the group that transfected p73 only (P<0.05). However, kinase-deficient Plk3 (K52R) had no significant effect on the luciferase activities induced by p21~(WAF1) and Bax promoters (P>0.05). RT-PCR showed that p73 increased the mRNA expressions of p21~(WAF1) and BAX (P<0.05). P1k3 decreased the expressions of p21~(WAF1) and Bax induced by p73 (P<0.05). Kinase-deficient P1k3 (K52R) had no significant effect on the expressions of p21~(WAF1) and Bax induced by p73 (P>0.05). Colony formation assay revealed that p73 decreased the colony formation of H1299 cells (P<0.05). P1k3 decreased the inhibitory effect of p73 on the colony formation of H1299 cells (P<0.05). Kinase-deficient P1k3 (K52R) had no significant effect on the inhibitory effect of p73 on the colony formation of H1299 cells (P>0.05).Conclusion: P1k3 can inhibit the transcriptional activity of p73, where as kinase-deficient P1k3 has no effect on the transcriptional activity of p73.

Objective To investigate the effect of pseudolaric acid B(PLAB) on growth of human gastric carcinoma cells in vitro.Methods The expression of PPAR? was detected by RT-PCR;the effect of PLAB on cell growth was tested by MTT;Hoechst33342/PI and DNA gel electrolysis were employed to examine apoptosis;cell cycle was checked by flow cytometry.Results When treated with 0.1～10 ?mmol/L PLAB for 72,the proliferation of MGC803 cells was significantly inhibited.The proportion of MGC803 cells at G2 phase was significantly increased when treated with 10 ?mmol/L PLAB after 48 h,and showed an apparent G2 phase arrest.After treatement with PLAB for 72,typical apoptotic changes were observed.The expression of PPAR? was at a low level in MGC803 cells and up-regulated when treated with 10 ?mmol/L PLAB for 48 h(P