Objective This study aimed at the effect of glutamate(Glu) on rat cortical pyramidal cells in cultured brain slices treated by threohydroxyaspartate(THA),an inhibitor of glutamate transporter.Methods The brain slice cultures were prepared using 1-day-old rat.Various concentrations of THA(50,100 and(500 ?mol/L))were added into the culture medium respectively.Cortical pyramidal cells' survival was evaluated by immunohistochemistry staining monoclonal antibody SMI-32,a nonphosphorylated neurofilament marker,Glu level in the culture medium was also measured.Ultrastructural picture of neuropathology were examined by EM microscopy. Results The brain slices in the control group maintain excellent organization and a stable population of pyramidal cells.THA caused a slow dose-dependent loss of cortical pyramidal cells and an increase of Glu level in the culture medium.THA of 100 ?mol/L resulted in a significant decrease in cortical pyramidal cells after culturing for 5 weeks.Pyramidal cells appeared vacuolar degeneration.Conclu-(sions Extra-cellular) Glu caused chronic excitotoxicity to cortical pyramidal cells.

Objective To explore the pathogeny,clinical characteristics,prognosis,and summarize treatment experience of acute cerebrovascular disease combined with multiple organ failure.Methods The clinical datas of 84 cases of acute cerebrovascular disease combined with multiple organ failure were retraspectively analyzed.Results In the 84 cases there were 38 cases with 2 organs failure,and the mortality was 55.26%.There were 25 cases with 3 organs failure,and the mortality was 72%.There were 20 cases with 4 or more organs failure,and the mortality was 100%.Conclusion The mortality of this disease is very high.Death is related to the character of the disease and the patients' age.Early recognisation and effective prevention and cure are the important tache to reduce death of MOF.

OBJECTIVE To investigate the clinical distribution characteristics and drug resistance of pathogens in hematology department ward during recent two years,and instruct clinical application of antibiotics reasonably.METHODS The bacteria were identified by VITEK-60 with GNI+,GPI and YBC.Drug sensitivity tests of bacteria were performed by K-B method.The results were analyzed by WHONET 5.3 software.RESULTS The results showed that 370 strains of pathogens were isolated from all detected samples.The composition ratio of Gram-positive bacteria,Gram-negative bacteria and fungi was 59.7%,32.4% and 7.8%,respectively.Coagulase negative Staphylococcus,Escherichia coli,Pseudomonas aeruginosa,Klebsiella pneumoniae and Acinetobacter baumannii were the most frequently isolated pathogens.The ESBLs producing strains in E.coli and Klebsiella spp were 59.3% and 24.2%,respectively.The MRSA in S.aureus and MRCNS in coagulase negative Staphylococcus were 18.2% and 87.8%,respectively.CONCLUSIONS Inspecting pathogens and studying the trend of their drug resistance are important to rationally select antibiotics,reduce infection incidence and mortality rate,and decrease the occurrence of drug resistant strains.

Objective: To study the inhibitory effect of VEGF antisense oligonucleotides (ASODN) on growth of Lewis lung cancer in C57BL/6 mice.Methods: Lewis lung cancer cells were cultured and implanted subcutaneously into 40 C57BL/6 mice, which were then divided into 4 groups: VEGF-ASODN treatment group, VEGF-SODN treatment group, BEGF-MODN treatment,and control group (normal saline). Mice in different groups were treated 24 hours after cell inoculation. The weight and volume of subcutaneous tumors was measured and the morphological changes of tumor cells was observed under microscope. VEGF protein and microvessel density were examined by immunohistochemistry.Partial tumor tissues were kept in liguid nitrogon.Results: The average tumor weights of the control, VEGF-ASODN, VEGF-SODN and VEGF-MODN groups were (7.33?0.71)g, (4.56?0.38) g, (7.59?0.32) g, and (7.62?0.39) g, respectively. The inhibition rates of tumor growth in VEGF-ASODN, VEGF-SODN and VEGF-MODN group were 43.8%, 5.5% and 3.1%, respectively. VEGF-ASPODN obviously inhibited the tumor cell growth and decelerated the tumor cell proliferation. Immunohistochemistry results showed that the expression of VEGF in ASODN group was remarkly lower than those in SODN group, MODN group and control group (P

Objective To explore the relevance between writing characteristic and therapeutic effect in schizophrenia and to discuss the influence of aggressive behavior on writing characteristic. Methods Re-coding the casual and fixed writing in admission, one week, two weeks, four weeks, eight weeks after treatment and rating Positive and Negative Syndrome Scale (PANSS) and Modified Overt Aggression Scale (MOAS). Choosing two characteristics, “relationship between font and grid lines” and “having big strokes or not”, and comparing before and after treatment. Results Eight weeks after treatment, the score of PANSS decreased. The condition of patients and the writing characteristic improved as well. The differ-ences of writing characteristics were statistically significant in patients with aggressive behavior before and after treatment (P<0.05). Conclusion The writing characteristic has relation with therapeutic effects and improved with therapeutic effects in aggressive patients.

Objective To study the value and the superiority of multi-slice spiral CT angiography of pulmonary artery in combination with deep vein of lower limb in diagnosing pulmonary embolism (PE). Methods CT pulmonary angiography (CTPA) and lower limb deep vein venography were performed in all suspected patients of PE with single or multi-slice spiral CT.The number and type of the embolus were calculated.Results Among the 44 patents of pulmonary embolism,34 patents had both PE and deep vein thrombo embolism (DVT) ,10 patients had only PE.The total ratio of the PE diagnosed with single slice spiral CT and multi-slice spiral CT was 12.1% and 19.0%, respectively , there were statistical significance, (P=0.004); the ratio of the PE upon deuto-segment pulmonary arteries was 25.3% and 29.7%, respectively, there was no statistical significance, (P=0.104); the ratio of the PE below deuto-segment pulmonary arteries was 1.1% and 10.2%, respectively, there was statistical significance, (P=0.000).Conclusion The technique of angiography of pulmonary artery in combination with deep vein of lower limb in diagnosing PE is a non-invasive, fast, sensitive and specific method for PE. Multi-slice spiral CT is superior to single slice spiral CT in the diagnosis of PE.

Objective To evaluate the platelet function in the patients with recurrent spontaneous abortion (RSA) ,to explore the correlations between Hcy and folic acid(FA)with mean platelet volume(MPV) and platelet distribution width(PDW) ,and to evalu-ate the application value of above indicators in predicting RSA .Methods Twenty-eight cases of RSA ,40 cases of healthy pregnancy and 50 healthy child-bearing women(control group) in our hospital were selected .The detection indicators included peripheral blood Hcy ,FA ,PLT ,MPV and PDW .Results The PLT and FA levels in the patients group were significantly lower compared with the normal pregnancy group and control group(P<0 .01) ,while the Hcy ,PDW and MPV were increased(P<0 .05);the various indica-tors had no statistically significant difference between the normal pregnancy group and control group (P>0 .05) .Blood Hcy was negatively correlated with PLT and FA ;Hcy and FA were negatively correlated in the normal pregnancy group ,other indicators had no obvious correlation(P>0 .05) .Conclusion The Hcy metabolism abnormality ,platelet parameter changes and their interactions may be related to RSA .The combined detection of Hcy ,FA and platelet parameters has a certain clinical significance to early diag-nosis and treatment of RSA .

Signal transducer and activator of transcription 3(STAT3)is an important member of STATs family and is highly expressed in many kinds of cancer.It regulates the tumor growth by influencing the apoptosis of tumor cells and tumor angiogenesis and becomes a new target for tumor therapy.This article reviews the structure of STAT3,the stimulating factors of STAT3,the tumor growth regulating pathway of STAT3,and its potential as a target for tumor therapy.

Objective To determine the sensitivity and specificity of ramification amplification method for detecting Shiga toxin and to determine the feasibility in detecting E. coli 0157: H7 and other shiga toxin-producing E. coli isolated from food and clinical specimens. Methods The sensitivity and specificity of RAM were compared with those of PCR by detecting synthetic Shiga toxin DNA target and isolates from food and clinical specimens. Results The lowest number of targets detected by RAM assay was 10 molecules. Several clinical isolates of E. coli 0157 :H7, Shigella dysenteriae and nonpathogenic E. coli were further tested. The results showed that E. coli 0157: H7 and Shigella dysenteriae were positive for shiga toxin gene,while nonpathogenic E. coli were negative. The results of RAM and PCR by detecting isolates were same. Conclusion RAM assay could be an alternative to PCR to detect STEC in food product and clinical specimens because of its high sensitivity and specificity , simplicity and isothermal amplification.

0.05). After experimental cerebral hemorrhage for 4 hours of model group, blood pressure and P O 2 were obvious increased( P 0.05), but they were lower than model group at the meantime ( P