Objective To establish serum pepsinogen (PG)reference range healthy crowd with latex-enhanced immunoturbi-dimetry of and explore its clinical application in stomach disease in Shanghai area.Methods Selected randomly 8 763 healthy adults who livied in Shanghai for more than 10 years and 269 patients with different stomach trouble confirmed by gastro-scope and pathology.By latex-enhanced immunoturbidimetry to detect the serum pepsinogen Ⅰ (PGⅠ)and pepsinogen Ⅱ(PGⅡ).Results The serum PGⅠ,PGⅡand PGⅠ/PGⅡlevel showed a skew distribution.The normal reference range was 24.6~98.97 ng/ml,≤21.7 ng/ml and ≥3.43.PGⅠand PGR values decreased significantly in both atrophic gastritis (PGⅠ=36.6 ng/ml,P=0.007,PGR=3.63,P<0.001)and gastric cancer groups(PGⅠ=20.6 ng/ml,PGR=3.2,P<0.001).PGⅠand PGⅡvalues increased significantly in digestive ulcer group(PGⅠ=83.6 ng/ml,PGⅡ=17.5 ng/ml,P<0.001),but PGR values decreased significantly (PGR=4.37,P=0.006).Conclusion Establishing serum pepsinogen refer-ence range of healthy crowd with latex-enhanced immunoturbidimetry in the Shanghai area can help to diagnose better the stomach trouble.

OBJECTIVE:To establish a method for quality control of Diedahuoxue film METHODS:Indentification of the ingredients in the film was performed by TLC and the content of emodin in film was detected by spectrophotometry at wavelength of 512nm RESULTS:The average recovery was 101 2%,and RSD=0 78% CONCLUSION:This method is good in repeatability and can be used for quality control of the film

Objective:To analyze data of 5604 cases who received health examination in our hospital ,and understand morbidities of overweight and obesity in these people and their relationship with blood glucose level . Methods :Morning venous blood was taken from the 5604 subjects undergoing health examination to measure blood glucose lev‐el ,and their body weight and height were measured at the same time ,then body mass index (BMI) was calculated and analyzed .Results:Among the 5604 subjects , prevalence rates of overweight and obesity were 42.1% and 13. 3% respectively ,and they were 50% and 16. 4% in men ,and 25. 4% and 6. 8% in women respectively .Among different age groups (except ≥71 years group) , prevalence rate of overweight in men was significantly higher than that of women , P<0.01 all;except ≥61 years subjects , prevalence rate of obesity in men was significantly higher than that of women ,P<0.01 .In overweight group ,risk relative (RR) of impaired fasting blood glucose and dia‐betes mellitus to those of subjects with normal body weight was 2.32 (95% CI 1.91~2.82) and 2.92 (95% CI 2.31~3.70) respectively ;in obesity group , RR of impaired fasting blood glucose and diabetes mellitus to those of subjects with normal body weight was 3.2 (95% CI 2.57~3.99) and 5.17 (95% CI 4.53~5.90) respectively , P=0.001 all . Conclusion:Overweight and obesity have become a common problem among our customer groups .Compared with those with normal body weight ,overweight and obesity greatly increase risk ratio of impaired fasting blood glucose .

Objective To investigate the clinical significance of combined detection of serum pepsinogen (PG) and anti-Hp IgG antibody in early screening for gastric ulcer .Methods 65 patients confirmed as gastric ulcer for the first time were selected as ulcer group ,while 65 healthy people in the same period as the control group .Transmission detection using latex enhanced immune nephe-lometry was adopted to detect their serum PG Ⅰ ,PG Ⅱ and the PG Ⅰ /PG Ⅱ ratio (PGR) was calculated .Enzyme-linked immu-nosorbent assay(ELISA) was used to detect serum anti-Hp IgG antibody .Results The average concentration of serum PG Ⅰand PG Ⅱof patients in the ulcer group were (167 .14 ± 22 .43) ,(26 .68 ± 11 .24) ng/mL ,respectively ,and PGR was 8 .07 ± 1 .19 .The average concentration of serum PG Ⅰand PG Ⅱof subjects in the control group were (52 .15 ± 20 .17) ,(11 .26 ± 7 .68) ng/mL ,re-spectively ,and PGR was 3 .48 ± 1 .92 .The differences of PGⅠ ,PGⅡand PGR between the two group showed statistical significance (P<0 .05) .The positive rate of serum anti-Hp IgG antibody of patients in the ulcer group was 80 .5% ,which was obviously higher than that in the control group(26 .3% )(P<0 .05) .The serum PGⅠ ,PGⅡand PGR of subjects in Hp infection positive group were markedly higher than those in the negative group(P<0 .05) .Conclusion Combined detection of serum PG and anti-Hp IgG anti-body may be used as an screening indicators for early gastric ulcer .

AIM:To predetermine the period of efficacy of Diedahuoxue film former METHODS:The chemical stability of Diedahuoxue film former was studied by the constant temperature accelerated test according to the principle of chemical kinetics Using the emodin in Diedahuoxue film former as an index,its content was determined by spectrophotometry The period of efficacy of Diedahuoxue film former was determined RESULTS:The results showed that the decomposition of emodin in Diedahuoxue film former accorded with the primary reaction CONCLUSION:At room temperature(25℃),it is predetermined that the period of efficacy of Diedahuoxue film former is 1 10 years

AIM:To seek a simple and reliable process for extracting effective components in Diedahuoxue film.METHODS: According to uniform design, five factors in extraction were optimized, i.e. ethanol concentration, reflux time, reflux times,ethanol volume and drug particle. The absorption value was taken as evaluating parameter in UV- spectrophotometry. RESULTS: The results showed that the best extracting conditions were 95% ethanol, ten times amount of ethanol, 0.2mm size of particle and reflux time of 1h with 4 repetitions.CONCLUSION:This process is simple and reliable.

Objective To observe feature of clinic and imageology in patients with unstable angina pectoris after radiation therapy to chest. Methods The study enrolled 58 patients who had unstable angina pectoris. Twenty-eight patients were in the postradiation therapy to chest group, 30 patients were in the common unstable angina pectoris group. All patients underwent multislice spiral CT(MSCT) examination and coronary angiography (CAG). After one year follow-up, the levels of brain natriuretic peptide (BNP) and ventricular wall motion score index (WMSI) were measured , the incidence of cardiovascular events were observed in all patients. Results Calcific, multi-vessel, thrombotic disease and dissection were significantly different between the two groups, when one year follow-up , the levels of BNP were higher in the postradiation therapy to chest group than those in the common unstable angina pectoris group [ (234.31 ± 121.39) ng/L vs (124.74 ±37.81) ng/L] WMST, incidence of the first heart failure, angina pectoris recurrence and revascularization were significantly different too (P< 0.05 ). Conclusions The complex lesions are major in the postradiation therapy to chest. Moreover, because radiation injures the heart valves and myocardium,these patients' long-term prognosis are worst.

Objective To investigate the correlation between the serum ghrelin levels and nutrition state in the patients with chronic obstructive pulmonary disease(COPD) ,as well as to explore the role of ghxelin in the nutri-tion metabolism. Methods Fifty-three COPD patients were observed, thirty-one of them were with malnutrition (group A), twenty-two COPD patients with normal nutrition status(group B), and twenty were healthy controls.Serum ghrelin, and nutritional parameters such as body mass index(BMI), ideal body weitht( % IBW), mid-upper arm cricumference(MAC), serum albumin(ALB), total lymphocyte counts(LYM) were determined. The correlation between ghrelin and nutritional parameters was analysed. Resuits The level of serum ghrelin in group A were sig-nificantly higher than those in group B and in healthy controls. And the serum ghrelin showed a negative correlation with BMI, % IBW, MAC, but there was no correlation between serum ghrelin level, ALB and LYM. Conclusion Ghrelin participated in the nutrition metabolism in patients with COPD, it would become much higher because of malnutrition in COPD.

Objective To measure the expressions of zinc finger protein A20 and nuclear factor (NF)-κB in cutaneous squamous cell carcinoma (CSCC) tissue and their correlation with human papillomavirus (HPV) infection.Methods Tissue specimens were obtained from the lesions of 43 patients with CSCC and normal skin of 21 healthy human controls.Gene chips were used to detect the DNA of 21 HPV subtypes in these specimens.The expression levels of zinc finger protein A20 and NF-κB were determined by immunohistochemistry.The correlation between clinicopathological features of CSCC and HPV infection was estimated by Spearman rank correlation analysis.Results No significant difference was found in the expression level of either zinc finger protein A20 or NF-κB among patients of different age groups,gender,and with CSCC of different primary sites or histological grade (all P ＞ 0.05).However,there was a significant difference in the expression levels of zinc finger protein A20 and NF-κB between 39 patients with histological grade Ⅰ or Ⅱ and 4 patients with grade Ⅲ (25.85 ± 3.84 vs.48.34± 7.69 for zinc finger protein A20,46.64 ± 8.93 vs.57.34 ± 10.02 for NF-κB,both P ＜ 0.05),and between 3 patients with nodular metastasis and 40 patients without (35.34 ± 6.02 vs.26.51 ± 4.09 for zinc finger protein A20,57.53 ± 13.32 vs.45.45 ± 9.64 for NF-κB,P ＜ 0.05 or 0.01).The expression rate was 2/8,5/13 and 1/3 for zinc finger protein A20 (P ＞ 0.05),3/8,10/13 and 2/3 for NF-κB (P ＞ 0.05),in CSCC tissue specimens infected with low-risk HPV subtypes,high-risk subtypes,concurrent low-and high-risk HPV subtypes respectively.In HPV-infected CSCC tissue,a significantly positive correlation was found between the staining scores for zinc finger protein A20 and for NF-κB p65(r =3.14,P ＜ 0.05).Conclusion The high expressions of NF-κB and zinc finger protein A20 may play an important role in the oncogenesis of CSCC induced by HPV infection.

OBJECTIVE:To evaluate the efficacy and safety of tolvaptan in the treatment of liver cirrhosis ascites with hyponatremia systematically,in order to provide reference for clinical drug use. METHODS:Retrieved from The Cochrane Library, PubMed,CNKI and Wanfang database,etc.,randomized controlled trials(RCTs)about tolvaptan combined with routine treatment plan(trial group)versus routine treatment plan with or without placebo(control group)in the treatment of liver cirrhosis ascites with hyponatremia were collected. The qualities of included studies were evaluated according to modified Jadad scale after extracting data. Meta-analysis was performed by using Rev Man 5.3 statistical software. RESULTS:A total of 16 RCTs were included, involving 1 271 patients.The results of Meta-analysis showed that serum sodium concentration[MD=6.51,95%CI(4.64,8.39),P<0.001],24 h urine volume [MD=1.36,95%CI(1.01,1.70),P<0.001],response rate of ascites and edema [RD=0.27,95%CI (0.20,0.35),P<0.001],body weight improvement[MD=-1.11,95%CI(-1.31,-0.91),P<0.001]and abdomen circumference improvement [MD=-2.13,95%CI(-2.96,-1.31),P<0.001] of trial group were significantly superior to those of control group, with statistical significance.There was no statistical significance in the levels of blood potassium,blood pressure,heart rate,TBiL,Scr or BUN between 2 groups before and after treatment(P>0.05). The level of ALT in trial group was significantly lower than control group,with statistical significance(P=0.003). Subgroup analysis showed that there was no statistical significance in 24 h urine volume when traditional diuretics were given only in control group or not used in two groups(P>0.05);the serum sodium concentration and 24 h urine volume of other subgroups were significantly higher than those of control group,with statistical significance(P<0.001). The incidence of ADR in trial group was higher than control group as dry mouth,thirst,frequent urination,insomnia,with statistical significance(P<0.05). Total incidence of ADR in trial group was slightly higher than control group,without statistical significance (P>0.05). CONCLUSIONS:Tolvaptan has good therapeutic efficacy for liver cirrhosis ascites with hyponatremia,can effectively improves serum sodium concentration,24 h urine volume,ascites and edema,body weight and abdomen circumference,but rarely affects blood potassium,heart rate,blood pressure,liver and renal function.However,ADR as thirst should be paid attention.