Objective To identify occupational hazard factors and determine the critical control points in a cigarette factory, to evaluate the feasibility and effectiveness of prevention and control measures, and to put forward suggestions. Methods The occupational health survey combined with field detection was used to identify and analyze the occupational hazards in the workplace of a cigarette factory in Shandong Province. Results The occupational hazard factors in the cigarette factory were tobacco dust，other dusts，phosphine，methane, carbon monoxide, hydrogen sulfide, chlorine, high temperature, power frequency electric field and noise. Except noise, other occupational hazard factors were within occupational exposure limits. The detection results of noise were 76.7~88.9 dB(A)，and the over standard rate was 24%. Conclusion The protective facilities and measures to control the level of dust and chemical poisons in the cigarette factory are feasible and effective. As the key control points of noise, it is necessary to further improve and optimize the anti-noise facilities and measures according to the key control points of occupational hazard factors to strengthen personal protection and prevent the occurrence of occupational diseases.