Objective To evaluate the value of DNA quantitative analysis in diagnosis of breast tumor.Methods The data of 128 cases diagnosed with breast disease from Feb.2005 to Jan.2006 in Department of Pathology in Wuhan Center Hospital were collected,among whom 72 cases had benign breast lesion,10 cases had boundary breast lesion and 46 cases had malignant breast disease.Automated DNA imaging cytometry was used to identify DNA indexes of the 128 cases.Results The positive rate was 1.39％ (1/72),30.00％ (3/10) and 78.26％ (36/46) respectively in benign breast lesion,boundary breast lesion and malignant breast disease.The difference had statistical significance (P ＜ 0.05).In invasive ductal breast cancer,the positive rate was 57.14％ (4/7),76.47％ (13/17) and 100％ (18/18) respectively for breast cancer of grade Ⅰ,Ⅱ and Ⅲ.Conclusion Automated DNA imaging cytometry is of some value in analyzing the malignant grade of breast cancer and predicting the prognosis of breast diseases.

Objective To construct nursing emergency management system of emerging infectious diseases for suiting the new situation of emerging infectious disease management needs. Methods The preliminary indicators of nursing emergency management system of emerging infectious diseases were formulated through the literature review and group discussion. The 3 rounds of Delphi questionnaire consultation were held among 20 experts in the field of infectious diseases. Results The experts' positive coefficients were 0.91,1.0 and 1.0,and authority coefficient was 0.82,while coordination coefficients were 0.272,0.311 and 0.320 (P< 0.05). Variation coefficients of various indicators were 0.09-0.22. Finally,the nursing emergency management system of emerging infectious diseases including 4 first-level indicators,17 second-level indicators and 66 third-level indicators was constructed. Conclusions The construction of scientific,systematic and standardized nursing emergency management system of emerging infectious diseases can improve the emergency disposition ability of nurses,establish efficient and reasonable human resources system,and improve the nursing management level of emerging infectious diseases.

Objective:To understand the status quo of post-traumatic growth and family environment of parents of children with hemodialysis and analyze the relationship between them.Methods:From April 2017 to May 2019, parents of 200 children with hemodialysis in Children's Hospital Affiliated to Zhengzhou University were selected as research objects by convenience sampling. The general information questionnaire, Post-traumatic Growth Inventory (PTGI) , Family Environment Scale-Chinese Version (FES-CV) was used for the survey. Pearson correlation analysis and stratified regression analysis were used to explore the relationship between parents' post-traumatic growth and family environment. A total of 200 questionnaires were issued and 183 valid questionnaires were returned, with an effective response rate of 91.5%.Results:The total score of PTGI of 183 parents of children with hemodialysis was (61.95±15.54) , and that of FES-CV was (49.27±7.38) . The total score of PTGI of parents of children with hemodialysis were positively correlated with family environment intimacy, emotional expression, independence, success, culture, entertainment, morality and religion and organizational subscale scores ( P<0.05) , and negatively correlated with scores of environmental contradictions and control subscales ( P<0.05) . The results of stratified regression analysis show that after controlling general data, intimacy and emotional expression in the family environment were the main influencing factors for the post-traumatic growth of the parents of hemodialysis children. Conclusions:The post-traumatic growth of parents of children with hemodialysis is closely related to the family environment. Clinical nurses should take targeted measures to help parents get support from within the family, help them actively cope with diseases and improve post-traumatic growth.