1.The changes of Th17 and Th22 cells in patients with Helicobacter pylori infection
Chunxiang YANG ; Qingju ZHANG ; Donglin JIANG
International Journal of Laboratory Medicine 2014;(21):2901-2902
Objective To investigate the changes of Th17 and Th22 cells in patients with Helicobacter pylori(HP)infection . Methods 66 cases of HP infection (HP infection group) and 30 cases of healthy people (control group) were selected .The levels of Th17 and Th22 cells in peripheral blood were measured by flow cytometry and the level of IL-17 and IL-22 in serum were meas-ured by ELISA .Results Comparing with the control group ,the levels of Th17 ,Th22 ,IL-17 and IL-22 were significantly increased in HP infection group(P<0 .05) .The correlation analysis showed that the levels of Th17 ,Th22 ,IL-17 and IL-22 were significantly positive correlated with DPM(P<0 .01) .Conclusion Th17 and Th22 cells were actived in patients with HP infection ,which caused the high expression of immune effector molecules ,and promoted the progress of immune damage .
2.Advances in the study of Candida albicans gene mutation on azole drug resistance
Chunxiang ZHU ; Pinghui GAO ; Yuanying JIANG
Acta Pharmaceutica Sinica 2010;45(7):821-6
Gene mutation of Candida albicans is one of the main causes for azole drug resistance. Different types of variation play different roles in promoting the process of drug resistance. ERG series of gene mutations primarily affect the ergosterol synthesis pathway. When the regulatory factors TAC1 for CDR1 gene and Mrr1 for MDR1 gene generate mutations, the expression level of drug efflux pump protein in Candida albicans may be changed. In addition, gene copy number variation is also gaining attention. Therefore, the research of mutation resistance-associated genes has a positive meaning to explore the mechanism of drug resistance in Candida albicans.
3.Application of Short Message Service for Community-based Pulmonary Rehabilitation
Yuyu JIANG ; Chunxiang ZHOU ; Jianfeng HAO ; Jiao HUA
Chinese Journal of Rehabilitation Theory and Practice 2016;22(5):596-600
Objective To apply short message service (SMS) for pulmonary rehabilitation in residence. Methods A total of 102 old pa-tients with chronic obstructive pulmonary disease (COPD) in stable situation were equally divided into three groups randomly. They all ac-cepted a two-week lesson of exercise project, and then took in the project for 22 weeks. Meanwhile, group A phoned to their experts once a week, group B was phoned by their experts once a week, and group C accepted SMS once a day and sent back to the experts. They were in-vestigated with Exercise Self Regulatory Efficacy Scale (Ex-SERS), modified British Medical Research Council Scale (mMRC) and COPD Assessment Test (CAT) before and after intervention. Results The score of Ex-SERS was the most in group C (F=179.53, P<0.001), with the interaction of time (F=387.09, P<0.001). While the scores of mMRC (F=8.754, P<0.001) and CAT (F=11.32, P<0.001) were the least in group C. Conclusion Application of SMS in the integrated management for pulmonary rehabilitation in residence may improve the self regu-latory efficacy of exercise, release the dyspnea and the influence on living in patients with COPD.
4.Imaging findings of Charcot joint
Quanfei MENG ; Chunxiang ZHOU ; Yingming CHEN ; Bo JIANG
Chinese Journal of Radiology 2001;0(05):-
Objective To analyze the MRI characters of Charcot joint, and to evaluate the diagnostic value of X-ray, CT, and MRI on Charcot joint. Methods Eight patients with 8 Charcot joints underwent X-ray, CT, and MR examinations. 6 of them had syringomyelia, 1 patient had injury of the spinal cord, and 1 case had diabetes. All 8 patients had sensory reduction or deficit in the sick extremities. Results There were two types of Charcot joint, hypertrophic and atrophic. Radiographic and CT features of hypertrophic joint (n=3) showed hyperostotic osteosclerosis and mammoth osteophytes in the sick bones, periarticular ossification,and articular disorganization. Radiographic and CT features of atrophic joint (n=5) showed extensive bone resorption (destruction), periarticular debris, and articular disorganization. Main MRI features of Charcot joint included hydrarthrosis within joint capsule, thickened, loose, and elongated joint capsule with para-joint, peri-diaphysis, and inter-muscular extension in a pseudopodia pattern. The irregular joint capsule wall was presented as mild hypointensity on T 1WI, slight hyper-intensity on T 2WI, and was markedly enhanced after Gd-DTPA was administrated, which was considered as a characteristic manifestation of the lesion. Soft tissue mass containing hypo-intense stripes on both T 1WI and T 2WI was commonly noted adjacent to the involved joint. Conclusion X-rays plain film is the first choice for the diagnosis of Charcot joint, and MRI is pretty useful in the diagnosis of Charcot joint.
5.Self efficacy theory applied in pulmonary rehabilitation in residence in elderly patients with chronic obstructive pulmonary disease
Yuyu JIANG ; Chunxiang ZHOU ; Jianfeng HAO ; Jiao HUA
Chinese Journal of Practical Nursing 2016;32(6):463-467
Objective To explore the effect of self efficacy combined with mobile health information service applied in pulmonary rehabilitation in residence in elderly patients with chronic obstructive pulmonary disease. Methods Sixty-eight elderly patients in stable situation were divided into the control group and the intervention group with 34 cases in each group accoridng to the random digit table. In the control group, the nursing intervention included health education and telephone follow-up. The intervention group adopted self efficacy theory combined with mobile health information service. The items such as persistence in pulmonary rehabilitation, Exercise Self-Regulatory Efficacy Scale (Ex-SRES) and health condition were assessed. Results After intervention for 12 weeks, the scores of persistence in pulmonary rehabilitation in residence in the intervention group scored 5.35 ±1.42, which was higher than that in the control group (2.03±1.40), the difference was significant (t=160.43, P<0.01). Ex-SRES of the intervention group scored 62.06±13.10,91.21±11.12, which were higher than those of the control group, 50.38±18.03,42.56±18.23, the difference was significant (t=9.32,176.88, P<0.01) . The interaction between treatment effects and time effect was significant in COPD Assessment of Tool (CAT) and Ex-SRES respectively(F=489.95,P=0.00;F=134.80, P=0.00). Comparing value of CAT in baseline (24.97±4.74,24.62±5.39) and at the end of the twelfth week(24.94±4.74,24.65±5.35), the difference was not significant in two groups respectively(P>0.05). The improvement of CAT was significant at the end of the twenty-forth week (21.90±4.67) in the intervention group (t=-12.09, P<0.01). Conclusions Self efficacy theory and the application of mobile health information services can help patients persist in long-term rehabilitation exercise training.
6.The scheme and application of educational reformation during pre-medical training of medical education for eight-year program
Zhangming WEI ; Xiaolei REN ; Jing LIU ; Yifan ZHU ; Chunxiang JIANG ; Hanchun CHEN ; Qingnan HE
Chinese Journal of Medical Education Research 2015;(8):780-782,783
Central South University began to implement the eight-year medical education in 2004, and has accumulated rich experience in the pre-medical education through continuous reform and improvement. Since 2012, Central South University has made a series of reformation, which is more conducive to the all-round development of medical students , on the pre-medical education . Through adding freshman courses, humanities courses, bilingual teaching courses, and applying aca-demic adviser institution, early scientific research training and open courses during the pre-medical education, Central South University has exercised and strengthened students' scientific research ability, the humanistic quality, English level, and made the pre-medical comprehensive examination for the shunt selection of students. Thus Central South University has improved the eight-year medical students' comprehensive quality, and provided an example of the reformation for medical education.
7.Prevalence of rhinitis and related factors among college students in Urumqi,the Xinjiang Uygur Autonomous Region
Mengmeng YANG ; Chunxiang ZHAO ; Ci YAN ; Yuanyuan FANG ; Hao ZHANG ; Mamatali AHMATJAN ; Li GAO ; Xiaofang JIANG
Chinese Journal of Primary Medicine and Pharmacy 2016;23(14):2103-2106
Objective To investigate the prevalence and the related factors of allergic rhinitis in Xinjiang College students.Methods Five universities'students in Urumqi were chosen as the research subjects,the question-naire survey was conducted by the method of cluster sampling,and the results were analyzed.Results 1 609 ques-tionnaires were valid from 2 000 questionnaires.The prevalence rate of allergic rhinitis was 36.8% in the five univer-sities in Urumqi.Prevalence rate increased with grade.The prevalence rates of drinking,regular cleaning nasal cavity, scrubbing their nose in allergic rhinitis were 43.2%,40.7%,53.8% respectively,which were higher than those in not drinking,not regular cleaning nasal cavity,not scrub nose,the differences were statistically significant (χ2 =6.945,9.679,100.845,all P <0.05 ).There was no significant differences in the prevalence of allergic rhinitis among college students and gender,nationality and BMI(all P >0.05).Conclusion Allergic rhinitis not only closely related to the grades and the environmental factors,but also has some connections with living habits(drink,clean nasal cavity and scrub noses).
8.The correlation between toll-like receptor 9 gene 2848G/A polymorphism and primary anti-neutrophil ;cytoplasmic antibodies associated small vasculitis
Baichan CAO ; Chao XUE ; Yunhua LIAO ; Chunxiang WU ; Qian PENG ; Xiaoli WEI ; Jiang ZHOU ; Kunpeng BU
Chinese Journal of Rheumatology 2015;(7):473-476
Objective To investigate the correlation between toll-like receptor 9 (TLR9) gene 2848G/A polymorphism and primary antineutrophil cytoplasmic antibodies (ANCA) associated small vasculitis (AAV). Methods A case-control study was performed among 135 patients diagnosed with AAV and 140 disease-free control and we test the serum biochemical parameter. Polymorphism was analyzed by polymerase chain restricted fragments length polymorphism. As for statistic method, according to the character of data, we performed t-test, chi-square test, Spearman grade related analysis and one-way ANOVA. Results ① The frequencies of AA, GG, GA genotype of TLR9 2848 in AAV patients were 14.07%, 38.52%, and 47.71%, respectively; ② Significant increase in IgM was observed in AA genotype than GG+GA genotype in AAV patients (F=4.561, P<0.05). ③ There was no significant difference between AI and CI in AA, GA and GG genotype in AAV patients (F=2.115, 0.760, P>0.05). Conclusion AA, GA and GG genotypes are detected in TLR9 2848G/A in patients with AAV in Guangxi, without significant correlation with susceptibility to primary AAV in Guangxi.
9.Determination of Acetamidopyrrolidone in Compound Huonaosu Capsules by RP-HPLC
Chunxiang ZHAO ; Zhuosong JIANG ; Chunshi LI ; Qiuming RU ; Guijie ZHANG ; Lianxiu ZHENG ;
Chinese Traditional Patent Medicine 1992;0(08):-
Objective: To establish the determination method of acetamidopyrrolidone in Compound Huonaosu Capsules with RP HPLC. Methods: Kromasil C 18 column was used. The mobile phase was methanol water(10∶90) with 0.6ml?min -1 of flow rate, and the detection wavelength was at 230nm. Results: The linear range of acetamidopyrrolidone concentration was from 0.17 to 0.78mg?ml -1 and correlation coefficient was 0.9995. The average recovery of sample was 99.9% and RSD and 0.62%(n=5). Conclusion: The method is simple, accurate and reproducible. It can be used for determination of acetamidopyrrolidone Compound in Huonaosu Capsules.
10.Preoperational nutritional status for the children with congenital heart disease and the influential factors
Chunxiang QIN ; Juan JIANG ; Ying LI ; Dianjun WANG ; Jiarui CHEN ; Siyuan TANG
Journal of Central South University(Medical Sciences) 2017;42(9):1066-1071
Objective:To investigate the preoperational nutritional condition for the children with congenital heart disease,and to analyze the relevant factors.Methods:According to the standards of WHO,the Z-scores was used to assess the nutritional condition for the children,and the generational information questionnaire,State-Trait Anxiety Inventory,Self-Rating Depression Scale and Parent Understanding Questionnaire were used to analyze the maternal factors.Results:Stunting,underweighting and wasting represented the poor nutritional conditions,which accounted for 28.6%,25.3% and 25.3%,respectively.Maternal accurate perception and the psychological problems such as anxiety and depression were the main relevant factors.Conclusion:The poor nutritional condition for the congenital heart disease was serious.It is very important to improve the maternal accurate perception and to relieve the maternal psychological problems for changing the nutritional condition through appropriate health education and effective intervention.