Objective To construct the risk model for healthcare-associated infection (HAI)with multidrug-re-sistant organisms(MDROs)in intensive care unit (ICU).Methods 836 patients who were admitted to ICU for more than 48 hours between October 2012 and September 2015 were analyzed retrospectively,logistic regression model of HAI was constructed,the model was conducted goodness of fit tests and the area under ROC curve analysis. Results Among 836 patients,incidence of HAI with MDROs was 14.23%(n=119).15 variables that were statis-tically significant in univariate analysis were included in logistic multivariate analysis,the results showed that the following variables entered into logistic regression equation:length of ICU stay (OR,2.493 [95%CI ,1 .816 -3.494]),underlying diseases (OR,1 .536 [95%CI ,1 .243 - 1 .898 ]),hypoproteinemia (OR,87.211 [95%CI , 36.165-210.304]),ventilator days (OR,1 .723 [95%CI ,1 .399-2.121 ]),fever(OR,20.639 [95%CI ,3.462 -123.043]),and primary pulmonary infection (OR,0.295 [95%CI ,0.133 -0.664]).Evaluation of model effect:sensitivity 95%,specificity 87.9%,the area under ROC curve 0.973.Conclusion Logistic regression model has a high goodness of fit in predicting HAI among ICU patients.

In recent years,the application of immune checkpoint blockades has brought dramatical revolution to the treatment of malignant tumors,which not only significantly increase the clinical benefits of partial advanced lung cancer,but also gradually change the landscape of available treatment options for patients with local advanced and early-staged lung cancer.But problems of immunotherapy about efficacy and safety follows.It is urgent to find dynamic biomarkers to identify the patients who are most likely to benefit from immunotherapy and to monitor tumor-specific immune responses.Several dynamic biomarkers have been identified,we will review some of lung cancer-related biomarkers,such as PD-L1 、TMB 、ctDNA 、TILs and peripheral blood biomarkers.

Objective:To investigate the effect of serum 25 hydroxyvitamin D (25OHD) levels in early pregnancy on glucose and lipid metabolism indicators and pregnancy outcomes in women with polycystic ovary syndrome (PCOS).Methods:A prospective study was conducted on 132 pregnant women with PCOS admitted to the Yanda Hospital in Hebei from January 2021 to June 2022. They were divided into four groups based on serum 25OHD levels: severe vitamin D deficiency group (12 cases with 25OHD<10 ng/ml), vitamin D deficiency group (28 cases with 25OHD: 10-<20 ng/ml), vitamin D deficiency group (55 cases with 25OHD: 20-<30 ng/ml), and vitamin D sufficient group (37 cases with 25OHD≥30 ng/ml). Four groups of serum glucose and lipid metabolism indicators [total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), fasting blood glucose (FPG), fasting insulin (FINS), insulin resistance index (HOMA-IR)] and poor pregnancy outcomes were compared.Results:As the degree of vitamin D deficiency worsens in early pregnancy, serum levels of TC, TG, LDL-C, FPG, FINS, and HOMA-IR gradually increased (all P<0.05), while serum levels of HDL-C gradually decreased (all P<0.05). The serum 25OHD level in early pregnancy of PCOS pregnant women was negatively correlated with serum TG, FINS levels, and HOMA-IR levels ( r=-0.523, -0.612, -0.354, all P<0.05), and positively correlated with serum HDL-C levels ( r=0.484, P<0.05). There was no significant correlation with serum TC, LDL-C, and FPG levels (all P>0.05). The incidence of adverse pregnancy outcomes in the vitamin D sufficient group was significantly lower than the other three groups [8.11%(3/37) vs 34.55%(19/55), 46.43%(13/28), 8/12, P<0.05], while the incidence of adverse pregnancy outcomes in the vitamin D insufficient group was significantly lower than that in the vitamin D severely deficient group (34.55% vs 8/12, P<0.05). Conclusions:With the decrease of serum 25OHD levels in early pregnancy, the disorder of glucose and lipid metabolism in PCOS pregnant women gradually worsens, and the incidence of adverse pregnancy outcomes gradually increases.

Objective To establish a study cohort of chronic hepatitis B (CHB) in Qidong and evaluate its baseline characteristics. Methods CHB outpatients of the Third People's Hospital of Qidong were invited to participate in baseline survey from January 1, 2016, including questionnaire survey, liver function detection, serum detection of HBV infection and upper abdomen ultrasound detection. Anticipated sample size was at least one thousand. Baseline data were inputted by EpiData 3.1 software and then cleaned and analyzed by SAS 9.3 software. Results As of 18 July, 2016, a total of 1006 participants had been enrolled into the current study, including 615 males with an average age of (44.26±9.97) years and 391 females with an average age of (46.66±11.17) years. The difference in family history of liver disease was not significant between males and females (P>0.05), while the differences in other key information, such as age, education level, tobacco consumption, alcohol drinking, tea consumption, and antiviral intervention, were significant between males and females (P<0.05). Among the key clinical parameters, such as ALT, HBeAg, HBsAg, HBV DNA, albumin, and width of splenic vein and portal vein, only the abnormal rates of ALT and total bilirubin levels were higher in males than in females, the difference was significant (P<0.05). Conclusion Outpatient department-based CHB cohort was established successfully in Qidong, and sub-cohort could be divided according to the differences on baseline characteristics.