Objective To investigate the risk factors of prehypertension among postmenopausal women.Methods2 592[(43±12)years old] health women were collected as the research object in Saihan District of Hohhot in Inner Mongolia Autonomous Region in April 2015,including 697[(58±6)years old] postmenopausal subjects and 1 895[(37±8)years old] premenopausal subjects.T test was used to compare means of blood pressure, fasting blood glucose (FBG), blood lipid, and body massive index (BMI) between postmenopausal group and premenopausal group and to compare prevalence of prehypertension, hyperglycemias, dyslipidemias, overweight, and obesity between two groups.Logistic Regression was implemented to analyze the relationship between different risk factors and prehypertension among postmenopausal women.Results Compared with premenopausal women, the systolic pressure, diastolicpressure, BMI, FBG, triacylglycerol (TG), low density lipoprotein (LDL) in postmenopausal women were significantly higher(P<0.05).Prevalence of prehypertension, impaired fasting glucose (IFG), diabetes, TG abnormalities, LDL abnormalities, overweight, and obesity in postmenopausal women were significantly higher than in premenopausal women(P<0.05).Age 55 to 59, 60 to 64 and above 65 years overweight, obesity, IFG and diabetes were independent risk factors of prehypertension among postmenopausalwomen.Conclusions Age 55 yearsand above, overweight, obesity, IFG and diabetes are the independent risk factors of prehypertension among postmenopausal women.

Objective To analyze the genetic diversity of Psammosilene tunicoides,an endangered and endemic medicinal plant,in southwest China.Methods The genetic diversity of seven representational populations of P.tunicoides including 137 individuals had been investigated by amplified fragment length polymorphisms(AFLP)maker technique.Results The genetic diversity had been revealed as follow:the Nei's genetic diversity index(He),Shannon's information index(I),percentage of polymorphic loci(PPB)were 0.243 4?0.179 1,0.373 5?0.248 5,and 82.30%,respectively at the species level;and 0.091 8?0.161 0,0.140 2?0.236 2,and 30.48%,respectively at population level.The genetic differentiation index(Gst)was 0.624 6 and genetic differentiation coefficient by Shannon's diversity(Ist)was 0.624 6.The result of dendrogram of seven populations indicated that Lijiang and Gejiu of Yunnan populations shared the maximum genetic identity,though they distributed in a relatively great geographical distance;Kunming population of Yunnan had the greater genetic distance from other populations.Nine characteristic fingerprint bands that can distinguish the different populations had been acquired.Conclusion The genetic diversity of P.tunicoides is relatively higher at the species levels,while lower within population levels,and a significant degree of genetic differentiation occurrs among the populations.There is little relativity between the relationship of populations and geographical distance.The combination of characteristic fingerprint bands between intraspecies and populations provide important basis data for germplasm resources diagnostics and plant breeding by AFLP maker technique.

Objective To screen the genes most relevant to lymph node metastasis of cervical cancer and identify the genes at the key knots of the regulatory network to provide the potential targets for cervical cancer intervention.Methods The transcriptional profiling database of TCGA was used, and random forests algorithm was adopted to rank the genes related to lymph node metastasis extracted from GeneCards database.STRING and Cytospace tolls were used to build the interactive regulatory network and identify the most weighted genes localized in the central of the network.DAVID platform was used to perform a functional annotation for the whole geneset.Results We ranked 2784 genes in respect to their potential contributions to lymph node metastasis of cervical cancer and identified the genes at the key knob.The genes related to cancer metastasis were enriched to cytokines pathway, MAPK pathway, wnt pathway, intercellular interaction, adhesive conjunction, cellular skeleton regulation, etc.Some of the identified key genes, like EGFR, NOTCH1, RHOA, etc. have been verified to be closely related cervical cancer metastasis in the basic and clinical research. Conclusion Random forests algorithm is useful, taking advantages of TCGA database, in enriching the genes playing significant role in cervical cancer metastasis.A majority of the genes in the analyzed geneset were indicated to be significantly correlated with lymph node metastasis.

Objective To explore the effects of family-centered care on negative emotion and quality of life in patients with acute coronary syndrome(ACS)based on "Timing It Right".Methods A total of 126 patients with ACS undergoing PCI therapy in the Cardiology Department of Wuxi Second People's Hospital from April 2016 to July 2017 were selected by convenience sampling method.All subjects were randomly divided into the intervention group(n=63)and the control group(n=63).A total of 121 patients competed the study,with 61 in the intervention group and 60 in the control group.The control group received routine nursing care and cardiology department follow-up.The intervention group was given routine care and family-centered care based on "Timing It Right" on the basis of the control group.Using Self-Rating Anxiety Scale(SAS),Self-Rating Depression Scale(SDS)and 36-Item Short Form Healthy Survey(SF-36)to assess the patients on admission,at discharge,3 month and 6 month after discharge respectively,so as to compare the effects between the two groups.Results The negative emotion of all the patients showed a gradual downward trend.The scores of SAS and SDS of the intervention group 3 months after intervention were lower than those of the control group,with statistical significance(P<0.05).The quality of life showed increased gradually with time in both groups.The scores of the general dimension of SF-36 were(72.19±19.86)and(66.74±18.23)in the intervention group and the control group respectively,and the difference was statistically significant(t=6.221,P <0.01).Conclusions The family-centered care based on "Timing It Right" can significantly improve anxiety,depression and quality of life in patients with acute coronary syndrome.

Objective:To explore the effect of Healthcare Effectiveness Data and Information System (HEDIS) nursing model on primary prevention knowledge and self-management behavior of patients with coronary heart disease.Methods:Using the convenient sampling method, 180 patients with coronary heart disease who were admitted to Department of Cardiology of the Second People's Hospital of Wuxi from April 2018 to April 2019 were selected as research objects. They were randomly divided into the control group and the observation group, with 90 cases in each group. The control group was given routine nursing, while the observation group was given HEDIS nursing intervention on the basis of routine nursing. The primary prevention knowledge questionnaire on coronary heart disease and Coronary Artery Disease Self-Management Scale were used to compare intervention effects.Results:After intervention, scores of primary prevention knowledge and self-management behavior of the observation group were higher than those of the control group, and the differences were statistically significant ( P<0.01) . Conclusions:Applying HEDIS nursing model to intervene patients with coronary heart disease can improve their primary prevention knowledge and self-management behavior, which can be widely promoted and applied in clinical practice.

Objective To investigate the role of macrophage migration inhibitory factor(MIF)on liver inflammation in mice with a-cute liver failure(ALF).Methods Eighteen male mice were randomly divided into normal group,model group and MIF antagonist ISO-1 group.D-galactose combined with lipopolysaccharide were used to induce ALF model.The serum levels of alanine aminotrans-ferase(ALT)、aspartate aminotransferase(AST)and total bilirubin(TBIL)were detected.The pathological changes of liver were ob-served by HE staining and the apoptosis of liver cells was observed by Tunel staining.The levels of MIF,CXC chemokine receptor 2(CX-CR2)and tumor necrosis factor-α(TNF-α)in mouse liver tissues were detected by enzyme-linked immunosorbent assay(ELISA)and the expression of MIF was observed by immunofluorescence.Results Compared with the normal group,the model group showed seri-ous liver tissue structure damage,increased hepatocyte apoptosis and decreased liver function.ISO-1 reduced liver tissue damage and hepatocyte apoptosis.The expression levels of MIF,CXCR2 and TNF-α in liver tissue of mice in model group was significantly in-creased,while ISO-1 could significantly reduce the expression levels of MIF,CXCR2 and TNF-α,with statistical significance(P＜0.05).Conclusion The expression of MIF is significantly increased in ALF,and inhibition of MIF can improve liver tissue injury and the expression of inflammatory factors,indicating that MIF plays an important role in ALF.

Objective To explore the effect of solution-focused approach on self perceived burden, anxiety and depression in elderly patients with coronary heart disease complicated with type 2 diabetes. Methods From January to June 2016, 76 elderly patients with coronary heart disease complicated with type 2 diabetes from inpatients department of Wuxi Second People's Hospital were selected as the research objects by convenience sampling method. The patients were divided into control group and observation group by random number table, each with 38 cases. Patients in the control group were given routine nursing and patients of the observation group were given solution-focused approach intervention on the basis of routine nursing. Self-perceived burden scale, self-rating anxiety scale and self-rating depression scale were used to evaluate theeffect of intervention.Results Compared with the score before intervention, there was statistical difference in the self-perceived burden score after intervention in two groups (P<0.05). Before intervention, there was no statistical difference in the self-perceived burden score between the patients of the two groups (P>0.05). After 3-month intervention, the self perceived burden score of the observation group was lower than that of the control group (P<0.05). After intervention, there was statistical difference in anxiety and depression score of the intervention group compared with that before intervention (P<0.05). Before the intervention, there was no statistical difference in anxiety and depression of the two groups (P>0.05). After 3-month intervention,the score of anxiety and depression in the intervention group was significantly lower than that in the control group (P<0.05).Conclusions Solution focused approach can reduce the self-perceived burden of the elderly coronary heart disease patients with type 2 diabetes and alleviate the patients' negative emotions effectively.

Objective To explore the influences of the path clinical health education on the self-management ability, health-promoting lifestyle, compliance, level of blood lipid in patients with PCI of one-day ward.Methods A total of 93 patients with PCI of one-day ward were selected as subjects and were randomly divided into observation group(n=47, the path of clinical health education) and control group(n=46, routine health education) by means of random number table. And then, we compared the self-care ability, health-promoting lifestyle, medication compliance, and level of blood lipid between two groups before and three months after intervention.Results After intervention, the proportion of low level, medium level, high level of self-care ability was 2.1%, 21.3%, 76.6% in observation group which was better than those(10.9%, 41.3%, 47.8%) in control group. The total score of health-promoting lifestyle in observation group [(157.40±17.86) points] was higher than that [(127.04±11.64) points] in control group. Moreover, the score of medication compliance in observation group [(27.98±3.89) points] was higher than that [(21.00±4.52) points] in control group. Besides, the level of total cholesterol, low density lipoprotein cholesterol, triglyceride in observation group was all lower than those in control. There were statistically significant differences in them between two groups( P<0. 05).Conclusions The path of clinical health education has an important role in improving health-promoting awareness and ability, self-management ability, compliance, level of blood lipid in patients with PCI.

Objective:To explore the effect of nursing intervention based on the theory of resourcefulness in young and middle-aged patients with myocardial infarction.Methods:From January to July 2021, convenience sampling was used to select 90 young and middle-aged patients with myocardial infarction in the Department of Cardiology, Wuxi Second People's Hospital as the research object. According to the random number table, the patients were divided into the intervention group and the control group, each with 45 cases. The control group conducted conventional nursing, and the intervention group carried out intervention based on the theory of resourcefulness on the basis of conventional nursing. The Chinese version of the Post-Traumatic Growth Inventory (C-PTGI) , the Chinese version of the Event-Related Rumination Inventory (C-ERRI) , and the Connor-Davidson Resilience Scale (CD-RISC) were used to evaluate the effect of the intervention.Results:After the intervention, the total scores of C-PTGI, CD-RISC and C-ERRI in the intervention group were higher than those in the control group, and the differences were statistically significant ( t=-7.799, -4.593, -4.433; P<0.01) . Conclusions:Nursing intervention based on the theory of resourcefulness can promote the post-traumatic growth of young and middle-aged patients with myocardial infarction, adjust the level of rumination, and improve the psychological resilience of patients, which can be popularized and applied in clinical practice.

Objective:To explore the protective effect of AGK2, a selective inhibitor of sirtuin 2 (SIRT2), on the mitochondria of L02 hepatocytes induced by thioacetamide (TAA) and its related mechanism.Methods:Human-derived hepatocyte line L02 cells were cultured in vitro. Different concentrations of SIRT2 inhibitor AGK2 were used as intervention drugs. Cell counting kit-8 (CCK8) was used to detect the effects of different concentrations of AGK2 on the activity of L02 cells, and the appropriate concentration was selected as the AGK2 intervention group. The normal group was not given any drug intervention. The model group was given 90 mmol/L TAA for modeling. Low, medium and high dose AGK2 groups were added with 1, 2 and 4 μmol/L AGK2, respectively 2 h before modeling. CCK8 was used to detect cell activity in each group. Morphological changes of cells were observed under inverted light microscope. The relative protein expression levels of isocitrate dehydrogenase (IDH1), malate dehydrogenase (MDH1), SIRT2 and fission protein 1 homologue (FIS1) were detected by Western blot. The expression of SIRT2 in cells of each group was observed by confocal laser scanning microscope. The mitochondrial membrane potential of cells in each group was observed under a fluorescence microscope. Results:When AGK2 concentration was 1, 2 and 4 μmol/L, the survival rate of cells were 98.05%, 95.76% and 91.65%, respectively, with no statistical significance compared with normal group (all P>0.05). When AGK2 concentration was 8, 16, 32, 64, 128 μmol/L, the cell survival rate was significantly decreased compared with normal group (all P<0.05). Compared with the model group, the L02 cells in low, medium and high AGK2 groups had better activity and adherence, and the floating cells were significantly reduced. The higher the concentration of AGK2, the better the cell activity and adherence, and the less floating cells. Compared with the model group, the red fluorescence of L02 cells in AGK2 group was enhanced, while the green fluorescence was weakened. The higher the AGK2 concentration was, the stronger the red fluorescence was, and the weaker the green fluorescence was. Compared with the model group, the fluorescence of SIRT2 in L02 cells of low, medium and high AGK2 groups was weakened, and the higher the concentration of AGK2, the weaker the fluorescence of SIRT2. The protein expressions of IDH1 and MDH1 in L02 cells of low, medium and high AGK2 groups were significantly higher than those of model group (all P<0.05), and were positively correlated with the concentration of AGK2 ( r=0.818, P<0.05; r=0.960, P<0.05); the protein expressions of SIRT2 and FIS1 were significantly lower than those of the model group (all P<0.05), and were negatively correlated with the concentration of AGK2 ( r=-0.992, P<0.05; r=-0.998, P<0.05). Conclusions:AGK2 can reduce the mitochondrial membrane potential stimulated by TAA in L02 cells, increase the protein expression of IDH1 and MDH1, and inhibit the protein expression of SIRT2 and FIS1 in L02 cells in a dose-dependent manner.