Objective: To observe the Prevention Action of Changtong Oral Liquid (CTOL) on the postoperative intestinal adhesions in rabbits. Method: Thirty-six male New Zealand rabbits were randomly divided into six equal groups (n=6): Normal control (group 1); model control (group 2); Simo Decoction (group 3); and CTOL minimal, medium and maximal dosages (group 4,5,6). Except group 1, the other rabbits were created as intestinal adhesive models. Group 1 and group 2 were orally fed with distilled water (20ml/kg), and group 3 to 6 with Simo Decoction 10ml/kg and CTOL in doses of 2.15g/kg, 4.30g/kg and 8.60g/kg respectively. On day 3 after surgery, the blood samples were collected to determine the fibrinolytic activity, and on day 9 the adhesion was graded. Result: CTOL could evidently reduce the severity of postoperative adhesions and increase plasma tPA activity and inhibit plasma PAI-1 activity. Conclusion: CTOL could prevent postoperative intestinal adhesion formation.

Objective To observe the effect of Changtong Oral Liquid (CTOL) on serum TNF-? and IL-4 levels in rats with postoperative intestinal adhesion. Methods Sprague-Dawley (SD) male rats were randomly divided into six groups: normal control, no treatment control, Simo Oral Liquid (SOL) group, and CTOL group (minimal, medium and maximal dosages). Except for normal control group, intestinal adhesions were produced in all the other rats with the method of Ellis. The blood samples of rats with CTOL medium dosage and no treatment group were collected on the 1st, 3rd, 5th, 7th and 9th day, and that of the rest was drawn on day 7 after surgery for the determination of TNF-? and IL-4 levels. Results The serum TNF-? levels were higher in no treatment rats compared with the normal controls (P0.05). Conclusions TNF-? was closely related to adhesion formation, and it may be regarded as a marker of adhesion formation.

Objective:To explore the effect of ChangTong oral liquid(CTOL) on cell cycle of cultured fibroblasts from normal peritoneum(NF) and adhesion(AF).Methods:15 SD male rats were randomly divided into 3 groups:normal serum group,2 CTOL groups(medium and high doses).After administration of medication at the different dosages for 7 days,serums were obtained from the abdominal arteries;the prepared tissues were cultured until outgrowth of fibroblasts.In our study,the passage 3-8 cells were used in order to maintain comparability;The fibroblasts in desired condition were plated in 10% FBS,then divided into three groups:rat serum normal group,CTOL groups(medium and high dose).Cells were cultured respectively for 12,24 and 48 h,and analyzed by flow cytometer.Results:Compared with serum normal group of NF,CTOL medium dosage could increase the proportion of NF in the G1 stage at 12,24 and 48h,but only CTOL high dosage had significance(P

Dachengqi Decoction is a classic prescription attacked by Yangming excessive syndromes in clinic, which has the effects of relieving heat, softening and dispersing knots, etc., and is often used in the treatment of gastrointestinal dysfunction caused by various diseases. This article reviewed the recent studies on the chemical compositions and pharmacological effects of Dachengqi Decoction in recent years. On this basis, combined with the "five principles" of TCM quality markers, the quality markers of Dachengqi Decoction were predicted and analyzed. It is suggested that emodin, Rhein, chrysophanol, aloe-emodin, synephrine, hesperidin, naringin, magnolol and magnolol can be used as quality markers of Dachengqi Decoction.

OBJECTIVE To identify the chemical components of Changtong oral liquid （CTOL），and to provide reference for the basic research and secondary development of its pharmacological substances. METHODS UHPLC-Orbitrap HRMS technique was adopted. CTOL sample was separated on a Hypersil Gold column with mobile phase consisted of 0.1% formic acid （containing 5 mmol/L ammonium formate）-acetonitrile （gradient elution）. The eluent was detected in positive and negative ion modes using an electrospray ionization source. The data was processed by Xcalibur 4.3 and Compound Discoverer 3.3 software. The primary and secondary mass spectra data of each compound were collected. The unknown compounds were identified according to the mass spectrometry library of the instrument and the network databases mzCloud，mzVault，etc. Through matching with the pharmacology database and analysis platform of the traditional Chinese medicine system，the chemical components could be attributed to the traditional Chinese medicine. RESULTS Fifty-three chemical components were identified and analyzed from CTOL，such as 24 flavonoids，8 quinones，5 phenylpropanoids，4 sugars and glycosides，5 organic acids，3 amino acids，1 alkaloid，1 phenolic and 2 other compounds. Among them，12 components were derived from Salvia miltiorrhiza，9 from Citrus aurantium，7 from Rheum palmatum，4 from Angelica sinensis，1 from Magnolia officinalis，16 from Glycyrrhiza uralensis，and 4 from many kinds of medicinal materials. CONCLUSIONS CTOL mainly contains flavonoids，quinones and phenylpropanoid compounds，and its chemical components mainly come from G. uralensis，S. miltiorrhiza and C. aurantium.

Objective: To evaluate the efficacy and safety of sofosbuvir (Nanjing Zhengda Tianqing Pharmaceutical Co., Ltd.) combined with ribavirin in patients with genotype 2 chronic hepatitis C virus infection. Methods: Treatment-naïve or treatment experienced genotype 2 chronic hepatitis C patients from sixteen research centers of China were screened. All subjects received once-daily dose of sofosbuvir (400 mg) combined with ribavirin (body weight < 75 kg, 1 000 mg/day, 400 mg in the morning and 600 mg in the evening; body weight > 75 kg, 1 200 mg/d, 600 mg in the morning and 600 mg in the evening) for 12 weeks. Patients were followed-up for a period of 12 weeks after discontinuation of treatment. Continuous variables were expressed as mean ± standard deviation. The proportion of subjects with virologic response at different follow-up time points and 95% confidence intervals were estimated by maximum likelihood ratio and Clopper-Pearson interval. Results: 132 cases with genotype 2 chronic hepatitis C virus infection from sixteen research centers of China were included, 12 cases of whom were associated with cirrhosis, and the remaining 120 cases were not associated with cirrhosis. One hundred and thirty-one cases completed the study, and one patient lost to follow-up at week 4 after the end of treatment. The sustained virological response rate was 96.2% (95% confidence interval: 92.37% - 99.16%) after 12 weeks of drug withdrawal. Virological relapse occurred in four cases. Of the 132 subjects enrolled in the study, 119 (90.2%) reported 617 adverse events during treatment, of which 359 (76.5%) were TEAE related to sofosbuvir and/or ribavirin. There were nine TEAEs of grade 3 and above, and six cases (4.5%) of them had six severe adverse events. Only one serious adverse event was associated with sofosbuvir and ribavirin (unstable angina pectoris). There were no adverse events leading to drug discontinuation or death. Conclusion Sofosbuvir combined with ribavirin has a high SVR rate in the treatment of genotype 2 chronic hepatitis C virus infection, and most of the adverse events occurred were mild with acceptable safety profile.