Objective To investigate the effect of c-fos antisense oligonucleotide on the phenotypes of vascular smooth muscle cells in venous autograftt. Methods The external jugular veins were grafted into abdominal aortic arteries in 20 Wistar rats which were divided into test group and control group randomly. The anastomosis and transplanted veins were coated with c-fos antisense oligonucleotide glue gel in the test group while the control group were merely coated with glue gel. The autografted veins were removed and measured by means of pathology and immunohistochemistry two weeks later. Meanwhile the conversion status of the vascular smooth muscle cells were observed with electron microscope. Results The myo-endothelial structure was observed clearly in test group while it was obscure in control group; the expression of c-fos、c-myc、PCNA in vascular smooth muscle cells was significantly decreased in test group. Conclusions The c-fos antisense oligonucleotide can influence the phenotypes of the vascular smooth muscle cells in autografted veins and inhibit the cells’ proliferation. All these indicate that it is a prospective genetic prophylactic therapy for the restenosis of autografted veins.

BACKGROUND: Effective drugs for improving vascular elasticity and revascularization are few for treating Leriche syndrome (chronic lower extremities ischemia) induced by abdominal aortic occlusion.Vascular outflow tract reconstruction in vascular prosthesis transplantation can rapidly improve blood supply in the distal extremities.OBJECTIVE: To evaluate the biocompatibility and outcome of vascular prosthesis transplantation for the treatment of Leriche syndrome.DESIGN: Controlled study before and after surgery.SETTING: Department of General Surgery of General Hospital of Chinese PLA and Department of Thoracic and Cardiovascular Surgery of Shijingshan Hospital.PARTICIPANTS: Sixty-two patients and sixteen patients with Leriche syndrome were enrolled in this study,who were respectively from Department of General Surgery of General Hospital of Chinese PLA between January 1995 and January 2007 and from Department of Thoracic and Cardiovascular Surgery of Shijingshan Hospital between January 2001 and January 2007.The subjects were 66 males and 12 females,whose age ranged from 46-75 years (averagely 58 years) and onset time from 6 months-8 years (averagely 2 years and 6 months).Informed consents were obtained from all patients.METHODS: After general anaesthesia or epidural anesthesia,vascular bypass was performed,including aortofemoral bypass in 63 cases and aortoiliac bypass in 15 cases.Vascular prostheses included polytetrafiuoroethylene (FIFE)vascular prosthesis in 18 cases and polyester vascular prosthesis in 60 cases.PTFE vascular prosthesis (Gore,USA) is a kind of inert material with low biological response.Its fibers with micropores arrange irregularly.Polyester vascular prosthesis (Intervascular,France) is a kind of knitting polyester products,which is made from high-purity cattle collagen Ⅰ with high biocompatibility.To inlay heparin molecules that can retain 4 weeks into the surface of vessels coated with collagen can prevent thrombogenesis and inhibit hyperplasy of smooth muscle cells (SMC).The type is IGK1608;internal diameter of the bole is 16 nun; internal diameter of the branch is 8 mm.Therapeutic prescriptions were approved by Hospital's Ethics Committee.Follow-up was performed for 3 months-5 years by vascular Color Doppler Sonography,spiral CT angiography (SCTA) three-dimensional reconstruction or digital subtraction angiography (DSA) in hospital.MAIN OUTCOME MEASURES: Biocompatibility of vascular prosthesis and outcome of vascular prosthesis transplantation.RESULTS: Three months after vascular prosthesis transplantation,thrombus was not detected on vascular anastomosis and in vascular prosthesis by Color Doppler Sonography and SCTA.Seventy-seven patients were followed up for over one year,and their stomas were unobstructed.Seventy-six patients were followed up for over five years,and the patency rate of stomas was 89% (68/76).No changes in blood plaque,lencocyte count,haematoglobin or liver and kidney function were found after transplantation.Seven days after surgery,affected extremities with ischemic symptom were improved.Three months later,ischemic symptom disappeared.CONCLUSION: With good biocompatibility of vascular prosthesis,vascular prosthesis transplantation in the treatment of Leriche syndrome has a good outcome following aortofemoral bypass and aortoiliac bypass.

Objectives:To investigate the effects of verapamil on serum induced proliferation of rabbit pigment epithelial(RPE) cells so as to search for simple and effective medicine on PVR. Methods:The rabbit RPE cells(passage 2 to 5) were cultured with various concentrations of verapamil in DMEM.The effects of verapamil on the cell cycle of RPE were analyzed with flow cytometry. Results:Verapamil significantly inhibited the serum induced proliferation of RPE cells, prevented RPE from G 1 phase transiting to S phase. Conclusions:Verapamil significantly inhibits RPE cell proliferation, and it may become a promising drug on PVR.

Objective To discuss the application and effect of Comprehensive Nursing Intervention on Patients with Hemiparesis of Cerebral Apoplexy. Methods A total of 80 cases of patients with hemiparesis of cerebral apoplexy were selected and divided into observation group and control group at random. The patients in two groups were given medicine treatment such as the control of intracranial pressure, blood pressure and blood sugar, brain cells nutrition, cerebral microcirculation improvement and etc. The patients in control group were given routine nursing intervention for 8 weeks, while the patients in observation group were additionally given comprehensive nursing intervention for 8 weeks. Results After 8 weeks’ nursing intervention, the total effective rate of recovery of neurological functions and the activity of daily living of patients in observation group was improved much better than that in control group(χ2=6.05 and 7.00,P<0.05). Meanwhile, the scores of life quality obviously were rose than before (P<0.05 or P<0.01), and the rising rate of patients in observation group was much higher than that in control group (P<0.05). Conclusion The comprehen-sive nursing intervention can obviously improve the recovery of neurological function, the activity of daily living and the life quality with hemiparesis of cerebral apoplexy, reduce the disability rate, and it is beneficial to the early recovery.

Objective The goal of this study was to carry out a trial of a novel disposable vacuum drainage bottle with speed drainage adjustment and a threaded catheter connector,as well as to further explore the effectiveness in drainage.Methods Twenty-seven patients who underwent continuous fluid drainage in the Coronary Care Unit(CCU)of our hospital between February and August 2023 were put on the trial.The patients were randomly assigned into a control group(12 cases,with 76 replacements of traditional vacuum drainage bottles over the catheterisation and an trial group(15 cases,with 83 replacements of the novelly modified disposable vacuum drainage bottle)by a simple random method.Patients in the control group had the drainage catheter connected to a traditional vacuum drainage bottle,while the patients in the trial group had drainage catheter connected to the novelly modified vacuum drainage bottle.The two groups were compared in terms of the number of bottle replacement,incidence of exudation/leakage/detachment,time for bottle replacement,cost of consumables for drainage,and satisfaction of patients and nurses.Results All patients completed the trial.The trial group exhibited a significantly lower incidence rate in exudation/leakage/detachment,shorter time for bottle replacement and lower cost of consumables for bottle replacement in comparison with those in the control group(all P＜0.05).Moreover,both patients and nurses in the trial group reported significantly higher satisfaction with the modified disposable bottle than the bottle used in the control group(both P＜0.001).Conclusions The novelly modified disposable vacuum drainage bottle overcomes the deficiencies of the traditional bottle,reduces the incidence of exudation/leakage/detachment over the drainage,cut down the cost of bottle replacement,and shorten the time for bottle replacement.The novel and modified disposable vacuum drainage bottle has received great appreciations from the patients and nursing staff.

Objective: To establish a set of rules for autoverification of blood analysis, in order to provide a way to validate autoverification rules for different analytical systems, which can ensure the accuracy of test results as well as shorten turnaround time (TAT) of test reports. Methods: A total of 34 629 EDTA-K2 anticoagulated blood samples were collected from multicenter cooperative units including the First Hospital of Jinlin University during January 2017 to November 2017. These samples included: 3 478 cases in Autoverification Establishment Group, including 288 cases for Delta check rules; 5 362 cases in Autoverification Validation Group, including 2 494 cases for Delta check; 25 789 cases in Clinical Application Trial Group. All these samples were analyzed for blood routine tests using Sysmex XN series automatic blood analyzers.Blood smears, staining and microscopic examination were done for each sample; then the clinical information, instrument parameters, test results and microscopic results were summarized; screening and determination of autoverification conditions including parameters and cutoff values were done using statistical analysis. The autoverification rules were input into Sysmex Laboman software and undergone stage Ⅰ validation using simulated data, and stage Ⅱ validation for post-analytical samples successively. True negative, false negative, true positive, false positive, autoverification pass rate and passing accuracy were calculated. Autoverification rules were applied to autoverification blood routine results and missed detection rates were validated, and also data of autoverification pass rate and TAT were obtained. Results: (1)The selected autoverification conditions and cutoff values included 43 rules involving WBC, RBC, PLT, Delta check and abnormal characteristics. (2)Validation of 3 190 cases in Autoverification Establishment Group showed the false negative rate was 1.94%(62/3 190)(P<0.001), autoverification pass rate was 76.74%, passing accuracy was 97.47%; Validation of 2 868 cases in Autoverification Validation Group, the false negative rate was 3.38%(97/2 868)(P=0.002), autoverification pass rate was 42.26%, passing accuracy was 92.00%; Validation of Delta check on 288 cases in Autoverification Establishment Group and 2 494 cases in Autoverification Validation Group showed the false negative rates were respectively 1.39% and 2.61%(P<0.001). (3)Three hospitals adopted these rules of autoverification for 25 789 blood routine samples, and found that the average TAT of blood routine test reports were shortened by 24min, 32min and 7min respectively, the rate of samples reported within 30min were elevated by 33%, 53% and 7%. The autoverification pass rates were 72%-74%. Conclusions The application of this set of 43 autoverification rules in blood sample analysis can ensure test quality while shortenTAT and improve work efficiency. It is worth pointing out that for the same analytical systems in this research, validation is necessary before application of this set of rules, and periodic validation is required during application to make necessary adjustment; for different analytical systems, as this research provide a way to establish autoverification rules for blood routine tests.Clinical labs may establish their own suitable autoverification rules on the basis of technological parameters. (Chin J Lab Med, 2018, 41: 601-607)