Objective:To investigate the effects of c-fos antisence olioneucleotide on the intimal proliferation of autogrfted veins with antisence technology.Methods:The external jugule veins were grafted into common carotid arteries in 20 rabbits and were divided into test group and control group randomly.The anastomosis and transplanted vein were coated with c-fos antisence olioneucleotide glue gel in the test group,while the contral group were merely coated with glue gel.The autografted veins were removed and measured by means of pathology and immunohistochemistry two weeks later.Results:The results show that the thickness of the venous intima,the degree of the vascular stricture,the expression of PCNA and the numbers of vascular smooth muscle cell were decreased in test group.Conclusion:The results suggest the c-fos antisence olioneucleotide can inhibit the intimal proliferation of the autografted veins.It is a prospective and idea genetic prophylactic therapy to intimal hyperplasia.

Objective To investigate the effect of c-fos antisense oligonucleotide on the phenotypes of vascular smooth muscle cells in venous autograftt. Methods The external jugular veins were grafted into abdominal aortic arteries in 20 Wistar rats which were divided into test group and control group randomly. The anastomosis and transplanted veins were coated with c-fos antisense oligonucleotide glue gel in the test group while the control group were merely coated with glue gel. The autografted veins were removed and measured by means of pathology and immunohistochemistry two weeks later. Meanwhile the conversion status of the vascular smooth muscle cells were observed with electron microscope. Results The myo-endothelial structure was observed clearly in test group while it was obscure in control group; the expression of c-fos、c-myc、PCNA in vascular smooth muscle cells was significantly decreased in test group. Conclusions The c-fos antisense oligonucleotide can influence the phenotypes of the vascular smooth muscle cells in autografted veins and inhibit the cells’ proliferation. All these indicate that it is a prospective genetic prophylactic therapy for the restenosis of autografted veins.

Objective To summarize the clinical experience of minimally invasive treatment (sclerosing therapy, intravascular intervention, laser coagulation, etc) for Klippel-Trenaunay syndrome (K-TS). Methods A total of 32 patients with K-TS were treated in this hospital from February 1989 to November 2004. Vascular embolization was used in patients with abnormal arteriovenous fistula or abnormal collateral arterial pathway. The insufficient valves of the deep veins were minimally invasively repaired. Laser coagulation was utilized for treating bulky varicosities. For angiomas and engorged venous plexus of the limbs, the sclerosing agent was injected. Results Varicosis, including reticular venous dilation, subsided completely. Angiography revealed an immediate disappearance of arteriovenous fistula and abnormal blood supply of the femur. The enlargement of involved limbs was diminished gradually. The angioma became completely sclerous, disappeared or decreased in size, without dwindling under pressure. In patients with venous valve reconstruction, Doppler ultrasonography showed no reflux. Follow-up for 1~7 years (mean, years) in all the 32 patients found no recurrence. Conclusions Minimally invasive treatment, including intravascular intervention, laser coagulation, sclerosing agent injection, mini-incision valve repair and so on, is effective for the management of Klippel-Trenaunay syndrome.

To summarize the experience of chronic ischemic disease in the lower limb using arteriovenous bypass in situ great saphenous vein without extirpation of venous valves.Methods: The operations of in situ great saphenous vein arterial bypass without venous valves extirpation had been done and the perioprative clinical manifestation had been observed in 11 patients with serious chronic ischemia of lower limbs.Results: The clinical manifestations of ll patients had been improved gradually.Conclusion: It may be a better operation of in situ great saphenous vein arterial bypass without venous valves extirpation,which is effective,simple,practicable,and deserved to be popularized in some cases.

Objective To investigate the influence of HIWI gene silencing in biological behavior of T24 cells and explore the possibility for HIWI gene to be used as the molecular target of inhibiting bladder carcinoma cell proliferation with gene transfection and RNA interference(RNAi) technique.Methods T24 cells were divided into transfection group with pGenesil-2-HIWI,transfection group with pGenesil-2-HIWI2263,transfection group with pGensil-2-control,and two control groups transfected with PEI only,and PBS only,respectively.T24 cells were transfected with shRNA expression vectors targeting HIWI gene by PEI,and the cell proliferation and cell cycle were measured by MTT assay and FCM.Results At the time of post-transfection 24 h,the inhibitory rate of cell proliferation in transfection groups were 32.60% and 26.09%,they were lower than that in control group(3.54%).At the time of post-transfection 48 h,the percentages of cells at S phase in transfection groups were(29.39? 3.27)% and(30.87?10.88)%,they were lower than that in control group(39.36%?2.09%)(P

Objective To investigate the changes of phosphorylated CREB in spinal cord dorsal horn of rats underwent chronic compression of dorsal root ganglia (CCD) and effects of intrathecally administer NOS inhibitor L-NAME,AG or 7-NI and cGMP analogue 8-Br-cGMP on the expression of pCREB in dorsal horn and the changes in thermal hyperalgesia of rats.Methods 90 male adult Wistar rats were randomly divided into L-NAME,AG,8-Br-cGMP,7-NI,Cremophor and PBS groups.Different NOS inhibitors were injected intrathecally by microsyringe at the 5th day after CCD,the thermal paw withdrawal latencies were measured before injection and 0.5,2.0,6.0,12.0,24.0 h after treatment,the anti-nociceptive effects of agents were compared.The expression of pCREB was detected by immunohistochemical analysis 2 h after intrathecal injection.Results CCD significantly increased the expression of pCREB and pCREB positive neurons located in all laminae of bilateral spinal cord.The expression of pCREB in ispilateral and contralateral spinal cord showed no significant difference.Compared with PBS group,there was significant decreasing in number of pCREB positive neurons in L-NAME,AG and 7-NI groups(P

0.05).CONCLUSIONS Piperacillin/tazobactam in the treatment of urethral stricture complicated with infection is significantly effective and safely.

Objective To investigate the expression and cellular localization of cyclin-dependent kinase 5 (Cdk5) in cerebral cortex after subarachnoid hemorrhage (SAH) in rats.Methods Fifty-two male Sprague Dawley (SD) rats were randomly divided into either a sham operation group (n =12) or a SAH group (n =40).The latter was randomly redivided into 6,12,24 h,and day 2 and 3 subgroups (n =8 in each group).A rat SAH model was induced by injecting fresh blood into the prechiasmatic cistern.Western blot and immunohistochemistry were used to detect the expression of Cdk5 in rat brain cortex.Double labeling immunofluorescence staining was used to detect the cellular localization of Cdk5 protein in cerebral cortex.Neuronal nuclear antigen labeled neurons,and glial fibrillary acidic protein labeled astrocytes.Results Western blot showed that the expression of Cdk5 protein was up-regulated at 12 hours after SAH (t =3.709,P =0.001),and it reached the peak on day 1 (t =3.475,P=0.002).Immunohistochemistry showed that the proportion of Cdk5 positive cell was also increased gradually after SAH,and the changes of time course were consistent with the results of Western blot,and it reached the peak on day 1 (t =4.320,P =0.000).Double labeling immunofluorescence showed that Cdk5 was mainly expressed in the neuronal cytoplasm in the sham operation group,and Cdk5 shifted to the neuronal nuclei in the SAH group.Cdk5 was mainly colocalized between astrocytes and neurons.Conclusions SAH up-regulates the expression of Cdk5 protein in cerebral cortex.Cdk5 may be involved in early brain injury after SAH.

From the perspective of clinical doctors, in the teaching idea, national policy, school policies and different stages of clinicians cultivation , the article introduced the concept of translational medicine in the process of the clinical doctors tralning in the United States. It described US medical schools' (U.S. Virginia University School of medicine, for example) implementation ap-proach of translational medicine ideas in different stages, such as before entering school, during the period of school, practice exam and physician clinician tralning. It provided the reference for the de-velopment of translational medicine education in the process of China clinician education and tralning, including:medical students' integration into the related clinical research in preschool through volunteer service, the choice of multiple combination model of clinical science and basic research, interdisci-plinary examination of medical practitioners and the provisions of the research work in resident and specialist tralning stage.

Objective To explore the effect of TIP30 gene on the growth inhibition of renal carcinoma cell line 786-0 and look for a potential therapeutic target for renal carcinoma.Methods TIP30 gene was amplified by reverse transcription-polymerase chain reaction(RT-PCR).A eukaryotic expression vector pcDNA3.1-TIP30 was constructed and transfected into 786-0 cells;pcDNA3.1(+)was also transfected as control.After transfection,the expression of TIP30 in 786-0 cells was detected by RT-PCR and Western blotting.The changes of cell proliferation and cell cycle were observed by MTT and FCM assay.Results The mRNA and protein expressions of TIP30 gene in pcDNA3.1-TIP30-transfected 786-0 cells were significantly increased than those in untreated and pcDNA3.1(+)-transfected cells(P0.05).The inhibitory rate of pcDNA3.1-TIP30-transfected 786-0 cells was significantly higher than those in untreated and pcDNA3.1(+)-transfected cells(P