Objective To determine the clinical significance of measuring the levels of 25-(OH) vitamin D3 [(25-(OH) D3)],immune globulins and serum alkaline phosphatase(AKP) in children with recurrent community-acquired pneumonia(CAP).Methods Serum samples were collected in children with recurrent CAP (recur-rent CAP group,n =38),first CAP (first CAP group,n =123),and age matched normal children (heath control group,n =39).The 25-(OH) D3 level,IgG,IgM,IgA and AKP levels were measured.Results The serum 25-(OH)D3 concentration of the recurrent CAP group was (47.87 ± 2.28)nmol/L,that of the first CAP group was (84.43 ± 4.12) nmol/L,and that of the heath control group was (91.2 ± 7.85) nmol/L,the differences among the three groups were statistically significant (t =3.716,8.881,all P ＜0.05).The differences of the serum IgA concen tration among the three groups had statistically significant differences (t =4.596,5.319,9.230,all P ＜ 0.05).But the serum IgG concentration among the three groups had no statistically significant differences(t =1.115,1.887,1.090,all P ＞ 0.05).The serum IgM concentration among the three groups had no statistically significant differences (t =0.804,0.942,0.918,all P ＞ 0.05).The serum AKP concentration among the three groups had no statistically signifi cant differences(t =0.277,0.661,0.866,all P ＞ 0.05).The serum 25-(OH) D3 levels in 161 cases of CAP patients were not correlated to any of the levels of IgM,IgG,IgA,or AKP.Conclusion The levels of 25-(OH) D3 and IgA are significantly lower in children with recurrent CAP,indicating that vitamin D deficiency may play a role in the pathogenesis of recurrent CAP.Prophylactic use of vitamin D supplementation may improve the immune function and reduce the development of recurrent CAP in children.

Objective:To investigate the expression of myeloid-derived suppressor cells (MDSCs) and cytokines in peripheral blood of patients with ankylosing spondylitis (AS).Methods:From June 2018 to June 2019, 50 AS patients (AS group) and 50 healthy people (control group) in Lishui People's Hospital were selected in this study.The number of PMN-MDSC and monocyte MDSC(M-MDSC) and the expression of cytokines (IL-2, IL-4, IL-6, IL-10, TNF-α, γ-IFN and IL-17) in the peripheral blood were detected by flow cytometry.The differences of MDSCs expression frequency in each group and its relationship with cytokine expression were analyzed.Results:The PMN-MDSC, M-MDSC and IL-6 in peripheral blood of the AS group were (0.22±0.08)%, (1.48±0.32)% and (20.74±5.14)ng/L, respectively, which were significantly higher than those of the control group[(0.06±0.02)%, (0.43±0.11)% and (2.52±0.53)ng/L], and the differences were statistically significant ( t=7.933, 6.310, 10.553, all P<0.05). Conclusion:IL-6 may be one of the reasons to promote the infiltration and recruitment of MDSCs, and the increase of MDSCs may be related to the occurrence and development of immune tolerance after ankylitis infection.

Objective To investigate the relationship and clinical significance between the concentration of 25-hydroxy vitamin D3 [25-(OH)D3 ]in the serum and community-acquired pneumonia(CAP)in infants.Methods The study recruited 98 infants.There were 68 cases of mild pneumonia and 30 cases of severe pneumonia.The con-centration of 25-(OH)D3 in serum,IgA and alkaline phosphatase(AKP)were measured.33 infants who accepted physical examination in the outpatient department were selected as control group at the same time.Results The serum 25-(OH)D3 concentration of severe pneumonia was (21.09 ±7.32)ng/mL,mild pneumonia was (25.77 ± 8.06)ng/mL and the control group was (37.13 ±6.94)ng/mL.The differences among the three groups had statisti-cally significant differences(t =9.18,3.72,5.34,all P <0.05).The differences of serum IgA concentration among the three groups had statistically significant differences (t =5.42,3.96,8.92,all P <0.05).But serum AKP concen-tration among the three groups had no statistically significant differences(t =0.86,0.58,0.47,all P >0.05).The prevalence of Vitamin D deficiency was 40.0%,which was higher than mild pneumonia patients(20.6%)and the healthy children(9.1%)(χ2 =8.43,17.55,all P <0.05).Conclusion The serum 25-(OH)D3 concentration and IgA of CAP patients were lower than healthy children,especially in severe pneumonia cases.The prevalence of Vita-min D deficiency was significantly higher than healthy children.There were no relevance between the serum concentra-tion of 25-(OH)D3 ,IgA and AKP.25-(OH)D3 plays an important role in the development of CAP.The low level of serum 25-(OH)D3 concentration may be one of the risk factors and has correlation to the severity of pneumonia.

OBJECTIVE To study the effects of the compatibility of Schisandra chinensis on Psoralea corylifolia -induced oxidative damage and endoplasmic reticulum stress in L 02 hepatocytes，and to provide reference for clinical use of the compatibility attenuation of P. corylifolia -S. chinensis . METHODS L02 cells were divided into blank control group ，P. corylifolia model group （1 200 μg/mL P. corylifolia ，calculated by crude drug ），P. corylifolia -S. chinensis low-dose，medium-dose and high-dose groups （1 200 μg/mL P. corylifolia combined with 600，1 200，2 400 μg/mL S. chinensis ，respectively，calculated by crude drug ）. After the cells in each group were cultured in culture medium or drug solution for 48 hours，the levels of aspartate aminotransferase（AST）and alanine aminotransferase （ALT）were detected ；the levels of glutathione （GSH），superoxide dismutase （SOD）and malondialdehyde （MDA）in cell culture medium were detected ；reactive oxygen species （ROS）level and mitochondrial membrane potential in cells were detected ；mRNA and protein expressions of glucose-regulated protein 78（GRP-78）and protein kinase R-like endoplasmic reticulum kinase （PERK）were detected. RESULTS Compared with blank control group ，the levels of AST，ALT，MDA and ROS ，mRNA and protein expressions of GRP- 78 and PERK were increased significantly in P. corylifolia model group （P＜0.05 or P＜0.01）；while GSH and SOD levels ，mitochondrial membrane potential were decreased significantly （P＜0.05 or P＜0.01）. Compared with P. corylifolia model group ，above indexes of P. corylifolia -S. chinensis low-dose， medium-dose and high-dose groups were all improved significantly （P＜0.05 or P＜0.01）. CONCLUSIONS The compatibility of P. corylifolia -S. chinensis can alleviate P. corylifolia -induced oxidative damage and endoplasmic reticulum stress of L 02 cells.

BACKGROUND: LY01005 (Goserelin acetate sustained-release microsphere injection) is a modified gonadotropin-releasing hormone (GnRH) agonist injected monthly. This phase III trial study aimed to evaluated the efficacy and safety of LY01005 in Chinese patients with prostate cancer. METHODS: We conducted a randomized controlled, open-label, non-inferiority trial across 49 sites in China. This study included 290 patients with prostate cancer who received either LY01005 or goserelin implants every 28 days for three injections. The primary efficacy endpoints were the percentage of patients with testosterone suppression ≤50 ng/dL at day 29 and the cumulative probability of testosterone ≤50 ng/dL from day 29 to 85. Non-inferiority was prespecified at a margin of -10%. Secondary endpoints included significant castration (≤20 ng/dL), testosterone surge within 72 h following repeated dosing, and changes in luteinizing hormone, follicle-stimulating hormone, and prostate specific antigen levels. RESULTS: On day 29, in the LY01005 and goserelin implant groups, testosterone concentrations fell below medical-castration levels in 99.3% (142/143) and 100% (140/140) of patients, respectively, with a difference of -0.7% (95% confidence interval [CI], -3.9% to 2.0%) between the two groups. The cumulative probabilities of maintaining castration from days 29 to 85 were 99.3% and 97.8%, respectively, with a between-group difference of 1.5% (95% CI, -1.3% to 4.4%). Both results met the criterion for non-inferiority. Secondary endpoints were similar between groups. Both treatments were well-tolerated. LY01005 was associated with fewer injection-site reactions than the goserelin implant (0% vs . 1.4% [2/145]). CONCLUSION: LY01005 is as effective as goserelin implants in reducing testosterone to castration levels, with a similar safety profile. TRIAL REGISTRATION ClinicalTrials.gov, NCT04563936.
Humans ; Male ; Antineoplastic Agents, Hormonal/therapeutic use* ; East Asian People ; Gonadotropin-Releasing Hormone/agonists* ; Goserelin/therapeutic use* ; Prostate-Specific Antigen ; Prostatic Neoplasms/drug therapy* ; Testosterone