Objective To investigate the effect of the decoction to help recover intestinal function on the intestinal mucosal barrier of rats with gut ischemia-reperfusion injury. Methods Forty-five SD rats were divided into three groups randomly: control group,gut ischemia-reperfusion injury group and decoction group.We detected the content of DAO and D-lactate in serum and lysozyme in perfusate of intestine in each group.We observed the bacteria translocation,the change of ileum mucous membrane in pathomorphology and intestinal villi. Results Compared with those in gut ischemia-reperfusion injury group,the contents of DAO and D-lactate and bacteria translocation ratio were all lower in decoction group(P

Objective To investigate the effect of Intestinal Function-recovering Decoction on the bacteria and endotoxin translocation and the content of malondialdehyde(MDA)and superoxide dismutase(SOD)in serum of rats with multiple organ dysfunction syndrome(MODS).Methods Fifty-seven SD rats were divided into four groups randomly:control group,MODS group,decoction group and ampicillin group.We detected the content of endotoxin in the peripheral vein,portal vein and ileum.Bacterial translocation,change of ileum mucous membrane in pathomorphology and the contents of MDA,SOD,ALT,AST,Cr and BUN in serum in each group were also examined.Results The hepatic and renal function and ileum mucous membranes were significantly damaged in MODS group,decoction group and ampicillin group.Compared with those in MODS group,serum MDA,ALT,AST,Cr,BUN and endotoxin in the peripheral vein,portal vein and ileum in decoction group were significantly lower(P0.01).It exhibited a negative correlation between serum MDA and SOD in MODS group and ampicillin group(P

Purpose:To investigate the effect of Flk-1、LR P and MDR1 genes in the carcinogenesis and development of lung cancer. Methods:The expression of Flk-1、LRP and MDR1 geneproteins in primary lung tumors were studied immunohistochemically. Results:of the 70 lung cancers, 29 cases (49.2%) were positive for MDR1 expression in NSCLCs and 2cases (18.2%) in small cell lung carcinoma(S CLC); 41cases (69.5%)had overexpression of LRP in NSCLCs and 3cases (27.3%) in S CLCs, there was a statistically significant correlation (P

Objective To examine the relationship of exposure and potential dose of human exposed to indoor and outdoor air pollutants. Methods The definition and calculation methods of exposure and potential dose were analyzed and compared. Personal exposure and potential dose of formaldehyde and carbon monoxide in indoor and outdoor air were calculated by scenario evaluation approach respectively, and the relationships between exposure and potential dose were analyzed also. Results Potential dose could much better accurately reflect the amount of air pollutants entering human body through respiratory tract than exposure did. Exposures and potential doses of human exposed to formaldehyde and carbon monoxide in indoor and outdoor air were significantly correlated in some cases, but not correlated well in other cases. Conclusion Potential dose might be more accurately linked to health effects of air pollutants than exposure did.

Objective To explore the peroxisome proliferator activated receptor gamma (PPARγ) expression characteristic of lymphocytes in patients with asthma .Methods Collected blood samples from healthy subjects (health control group) and asthmatic patients(asthmatic group) before treatment ,2 and 4 days after treatment .Expression levels of PPARγtested with Q-PCR .Analyzed eosinophil percentage of induced sputum ,IL-5 concentration in sputum supernatant measured with ELISA kits .Results Compared with healthy control group ,the eosinophil percentage and IL-5 concentration were higher in asthmatic group before treatment ;meanwhile the expression level of PPARγwas at the lowest .After treatment ,PPARγgradually increased accompanied with eosino-phil percentage and IL-5 concentration gradually decreased .Conclusion Asthmatic patients had a lowest PPARγ expression level . Their recovery perhaps attributed to the up-regulation of PPARγin lymphocytes .The anti-inflammatory effect of PPARγachieved might be via inhibiting the function of Th2 cells .

Education should include two parts:teaching and learning.Therefore,during the course of medical ehtics teaching,if we want to get better teaching effect,besides the factor of teachers,we must make the students get interest in the studying of medical ethics and arouse the students' initiative and enthusiasm of studying.This thesis would discuss that in the following respects:enhancing the students'initiative and enthusiasm of studying;teacher's deep love for education enterprise and for students;person of exemplary virtue,example is better than precept;performing elicitation teaching method of developing the student's initiative and strictly avoiding cramming method of teaching

Weanling female rats were fed 12% lard diet supplemented with 2 g cholesterol and 60 mg cholic acid per 100 g diet for 52 days. Blood cholesterol levels were then analyzed for individual rat. Animals were divided into 4 groups according to their blood cholesterol levels and body weights. One group of animals, regarded as negative control, was killed immediately. The other 3 groups were fed 12% level of synthetic oil, lard or soybean oil diet respectively without cholesterol and cholic acid supplementation for 3 weeks. During the 3 weeks of rehabilitation, the following parameters were measured: body weight and serum cholesterol levels, every week; food intakes, every other day; high density lipoprotein cholesterol (HDL-C) levels at the beginning and the end of the rehabilitation period; fecal steroids excretion, including neutral and acid steroids from the 2nd to the 4th day of rehabilitation; liver weights, liver fat contents and liver cholesterol levels after animals were killed.The results showed that no significant effects on body weights, serum cholesterol levels, liver weights and liver fat contents of different kinds of dietary fat were observed during the rehabilitation period of hyperch-olesterolemia. But synthetic oil, as well as soybean oil diet caused a lower liver cholesterol levels, and higher levels of both serum HDL-C and fecal neutral steroids.

OBJECTIVE To realize the disinfectant effect of medical instrument by acidic electrolyzed oxidizing water(EOW).METHODS Two groups of subjects participated in this study,and chlorinated disinfectant is used in the control group. Adopting stability test to measure effective chlorine and pH,and to compute the bacterium elimination rate after disinfection. Contrast the result with the experimental group.RESULTS The pH mean value and the effective chlorine mean value of two groups before and after instrument immersing in had no remarkable differences. Medical instrument sterilized by EOW for 5 minutes,the bacterium eliminative rate was 100.0%,while sterilized by the chlorinated disinfectant needed 30 minutes. Sterilizing effects of two groups were the same,but sterilizing time was obviously short in experimental group.CONCLUSIONS The acidic electrolyzed oxidizing water can be used in medical instrument's disinfection. The effect is good,and the process is fast. EOW is better than chlorinated disinfectant in saving of resources economizing.

The aim of this experiment is to investigate the effect of teaseed oil which contains about 76% oleic acid on blood lipids and platelet function. Adult Newzealand female rabbits of 2-3 kg body weight were divided into 3 groups of 6-8 animals each. Stock diet was given with 5 ml of coconut, teaseed or safflower oil to each group plus 0.5g of cholesterol by using stomach tube every day for 8 weeks. The results showed serum total cholesterol (TC), LDL-C, platelet aggregation and plasma TXB2 level were significantly reduced in teaseed oil group as compared with that of coconut oil group. Serum TC, LDL-C were lowered too in safflower group, but it was not significantly different from that of coconut oil group. Ratio of serum HDL-C/TC in teaseed oil group was significantly higher than other two groups, and TXB2/6-keto-PGF1a ratio was decreased about 30% when compared with the two other groups, but with no statistical significance.Male Wistar rats were divided into 4 groups of 30 animals each and were fed for 9 weeks with semisynthetic diets containing 15% coconut, teaseed or safflower oil and 1% cholesterol respectively. Teaseed oil was found to cause a lower level in platelet aggregation and a significant reduction in plasma TXB2/PGF1a ratio. Analyses of fatty acid composition of platelet membrane showed the MUFA content was much higher and that of the ?-6 PUFA was obviously less in teaseed oil group. The results together with that obtained in rabbit experiment clearly indicated MUFA (mainly oleic acid) exerting beneficial effect on the prevention of cardiovascular diseases. Its possible pathway was discussed in the text.

Objective To observe the prevalence of anionic trypsinogen (PRSS2) gene G191R mutation in patients with acute pancreatitis (AP) and chronic pancreatitis (CP),and to investigate the effect of PRSS2 gene G191R mutation on susceptibility to pancreatitis.Methods The blood samples of 82 patients with acute pancreatitis,73 patients with chronic pancreatitis and 138 healthy subjects were collected,and genomic DNA was extracted.Nest PCR were performed to amplify PRSS2 gene and restriction fragment length polymorphism (RFLP) was followed by using Hpy188Ⅲ to distinguish the G191R mutation.DNA sequencing analysis was performed to confirm the mutation status.Results The size of nest PCR products was 436 bp.RFLP2 produced 309 bp and 127 bp fragments,which were resulted from PRSS2 gene G191R mutation (GGA →AGA).DNA sequencing analysis of the PCR products further confirmed the PRSS2 gene G191R mutation.Five of eighty-two(6.1％) patients with acute pancreatitis had PRSS2 gene G191R mutation (OR=0.682,95％ CI 0.231 ～ 2.010); one of seventy-three (1.4％) patients with chronic pancreatitis had the mutation (OR =0.145,95％ CI 0.019 ～ 1.145),and the corresponding value in healthy group was 8.7％ (12/138).The G191R mutation rate in patients with chronic pancreatitis was significantly lower than that in healthy group (x2 =0.432,P =0.035),but the G191R mutation rates were not significantly different between AP group and healthy group (x2 =0.487,P =0.485).Conclusions PRSS2 gene G191R mutation facilitates the degradation of anionic trypsin,and may reduce the incidence of chronic pancreatitis.