Objective To explore the risk factors of stress urinary incontinence (SUI) after vaginal delivery,and provide some clinical reference for its prophylaxis and treatment.Methods One hundred and nineteen patients had SUI after vaginal delivery (SUI group),and 217 patients had not SUI after vaginal delivery (control group).The relevant risk factors of SUI were analyzed by Logistic regression analysis.Results Single factor analysis results showed that age ≥ 30 years old,body mass index (BMI) ≥ 25 kg/m2,number of abortion ≥2 times,episiotomy,before or during pregnancy having urinary incontinence,postpartum increased abdominal pressure,history of constipation and neonatal weight in SUI group weresignificantly higher than those in control group [67.2％ (80/119) vs.39.6％ (86/217),65.5％ (78/119) vs.47.5％ (103/217),23.5％ (28/119) vs.13.4％ (29/217),31.1％ (37/119) vs.16.1％ (35/217),46.2％(55/119) vs.31.3％(68/217),28.6％(34/119) vs.15.2％(33/217),23.5％(28/119) vs.13.4％(29/217),(3.5 ± 0.4) kg vs.(3.2 ± 0.2) kg,P＜ 0.01 or ＜ 0.05].Multiplicity results showed that number of abortion ≥2 times,episiotomy,before or during pregnancy having urinary incontinence,postpartum increased abdominal pressure and high neonatal weight were independent risk factors of SUI (P ＜ 0.05).Conclusions Many abortion,episiotomy,before or during pregnancy having urinary incontinence,postpartum increased abdominal pressure and high neonatal weight are independent risk factors of SUI.Necessary intervention for the above risk factors has an important meaning,which may reduce the incidence of postpartum SUI.

Objective To investigate the correlation between human parvovirus (HPV) B19 and spontaneous abortion.Methods Participants included 30 cases of spontaneous abortion patients (patients group) and 30 normal pregnancy (control group).The potential risk of spontaneous abortion factors were recorded.Extracting all subjects cubits venous blood,HPV B19 DNA was tested by nested polymerase chain reaction,and HPV B19 IgM antibodies were determined by ELISA.The more factors analysis were performed by Logistic multiple regression analysis.Results There were significant differences in gravidity more than 2 times,combined with hypertension,combined with diabetes,HPV B19 DNA positive expression between patients group and control group [43.3％(13/30) vs.13.3％(4/30),33.3％(10/30) vs.6.7％(2/30),30.0％ (9/30) vs.6.7％ (2/30),36.7％ ( 11/30 ) vs.3.3％ ( 1/30)] (P ＜ 0.05 ).Logistic multiple regression analysis showed that gravidity more than 2 times,combined with hypertension,HPV B19 DNA positive expression were the independent risk factors of spontaneous abortion (OR =1.85,1.95,4.85,P ＜ 0.05).There was significant difference in the risk between early spontaneous abortion and lately spontaneous abortion in HPV B19 DNA positive expression patients (P ＜ 0.05 ).Conclusions HPV B19 infection is the natural abortion independent risk factors,need to early detection of pregnancy,and carry out necessary intervention in pregnant women,in order to reduce the occurrence of natural abortion.

Objective To compare the value of 64-slice CT and 256-slice spiral CT in the diagnosis of pul-monary embolism.Methods A retrospective analysis CT pulmonary angiography of pulmonary embolism clinical cases,65 cases were in our hospital ,256-slice CT and 64-slice CT angiography were performed within 3 days of onset . The two kinds of inspection equipment for pulmonary embolism detection rate were analyzed and compared , and its image SNR, in contrast to noise ratio ,average scan time and the average dose of contrast agent used for comparison . Results The two devices display rate of pulmonary embolism was 100.0%,in the noise,image SNR,contrast to noise ratio,scan length,the 256-slice CT and 64-slice CT was no significant difference (P>0.05).The average 256-slice CT contrast agent was (4.45 ±1.37) mSv,64-slice CT was (5.68 ±1.06) mSv,there was significant difference between the two (t=2.77,P<0.05),an average of 256-slice CT scan time was (3.68 ±1.29)s,64-slice CT was (7.61 ± 1.40),there was significant difference between the two (t=11.33,P<0.001).Conclusion 256-slice CT and 64-slice CT can be used as an effective means of clinical diagnosis of pulmonary embolism ,but compared with 64-slice CT,the 256-slice CT can significantly reduce the scan time ,reduce the amount of radioactive contrast agent ,which has certain advantages .

Object To establish cell suspension culture system in leaves of seedling of Pueraria lobata (Willd.) Ohwi Methods The callus induced from leaf explants of P.lobata seedling were suspended in liquid medium. The time courses of cell growth and yields of puerarin and isoflavones were studied Results The log phase of the cell cultures' growth was from 1st d through 5th d. The dry weight accumulation reached highest on 8th d. The production of puerarin was highly coupled with the cell growth. The yield of puerarin reached highest on 8th d. However, the accumulation of total isoflavones was different from that of puerarin. The yield of total isoflaones reached the highest on 10th d Conclusion The yield of total isoflavones in cell suspension culture system is higher than that in wild plants, while the yield of puerarin is lower than that in wild plants

Objective To investigate the retroperitoneal ganglioneuroma ( GN) imaging findings ,and analyze its enhanced features .Methods 37 patients with retroperitoneal GN confirmed by pathology were chosen as subjects , the imaging features were analyzed and were compared with the pathologic results .All 37 cases underwent CT scan ,22 patients underwent CT enhance scan .Results 37 cases of retroperitoneal GN ,the lesions were located in the right side in 23 cases,14 cases located on the left.All lesions were demarcated,round or oval in 19 cases,18 cases of irreg-ular shape.CT scan 26 cases of 34 lesions of uniform density ,and density close to cystic lesions ,11 cases of 17 une-ven density,showed a little patchy,small strips calcification.Enhanced arterial phase lesions no enhancement ,venous phase and delayed phase showed progressive enhancement .Conclusion Retroperitoneal GN has certain characteristic imaging findings and strengthen the characteristics contribute to its diagnosis and differential diagnosis .

Objective To investigate the hospital distribution and drug resistance of pathogens of venous catheter-related infec-tion,so as to provide basis for appropriate usage of antibiotics in clinic.Methods The culture and drug susceptibility results of 369 venous catheter were analyzed retrospectively from 2009 January to 2012 December.Results 161 strains of pathogens were detec-ted among 369 venous catheter,the positive rate was 43.6%.There were 83 strains of Gram positive bacteria,accounting for 51.6%,and Staphylococcus epidermidis had the highest positive rate.There were 63 strains of Gram negative bacteria,accounting for 39.1%,and Klebsiella pneumoniae and Bauman Acinetobacter were main isolated bacteria.There were 15 strains of Fungi,ac-counting for 9.3%,and Candida parapsilosis had the highest positive rate.There was none Gram positive bacteria resistant to van-comycin and linezolid.Carbapenems remained high activity against Gram negative bacteria.Conclusion Staphylococcus epidermidis and Klebsiella pneumoniae are mainly detected in venous catheter in the hospital infection.

Objective To investigate the correlation between recurrent spontaneous abortion and karyotype correlation,and provide reference for clinical diagnosis and treatment of spontaneous abortion.Methods 51 couples peripheral blood lymphocyte were collected and cultured from March 2010 to March 2013 in the region.Karyotype analysis were performed to observe the distribution of the rate of chromosomal abnormalities situation.Meanwhile the correlation between the number of abortions and chromosomes were compared.Results Among the 102 subjects,32 cases(31.37％)of them were found chromosomal abnormalities in RSA.The ratio of polymorphic mutation accounted for 56.25 ％ of the chromosome abnormality.accounting for 17.65 ％ the total.Which was significantly higher than other chromosomal abnormalities(P＜0.05).Among the RSA and chromosomal karyotype cases,females accounted for 68.75 ％,which was significantly higher than males (31.25 ％)(P＜0.05).46XY(Y≥18)has the highest incidence in chromosome polymorphism,accounting for 33.33 ％,while abortions accounting for 30.77 ％.RSA number and incidence of chromosomal abnormalities to miscarriages three times the highest rate of abnormal,accounting for 43.48％.Conclusion Chromosomal abnormalities is an important factor in recurrent spontaneous abortion.Recurrent abortion is not only related to the structure of chromosomes,but also has a certain relationship with the variation of chromosome polymorphism.

Objective To discuss the diagnostic performance of MSCT in cystic nephroma(CN).Methods The MSCT findings of CN in 7 patients proved by operation and pathology were retrospectively reviewed.Results All of 7 cases were isolated cystic lesion, including 5 males and 2 females.MSCT scanning showed cystic tumor in the renal parenchyma with well-defined boundary.All cases showed regular cystic wall and septa with no attached cystic wall nodule.The cystic wall and septa appeared homogeneous enhancement on contrast-enhanced CT,while the cystic space showed inhomogeneous density and lack of enhancement.Conclusion The MSCT findings of CN may benefit the definite diagnosis.

Objective To establish the HPLC fingerprints for the guality control of ascidian Styela plicata.Methods The HPLC fingerprints of ten batches of samples were obtained using ZORBAX Bonus-RP(4.6?250mm,5?m) column with a mix of acetonitrile and water with 0.01%TFA as mobile phase in a gradient mode,the detection wavelength was 254nm and the temperature was 30℃.Results The experiment and analysis were carried out on ten samples,the standard HPLC fingerprint pattern method and 13 characteristic diffraction peaks of Styela plicata were obtained.The proposed method was precise,reproducible and steady.Conclusion This reliable and convenient method can be used for the identification and quality control of Styela plicata.

BACKGROUND: Triiodothyronine (T3) is an important regulation factor at the critical period of brain development. It maybe control the successive differentiation during the development of central nervous system (CNS).OBJECTIVE: To monitor the differentiation of neural stem cells (NSCs) induced by T3 and the thyroid hormone receptor (TR) mRNA expression changes.DESIGN: Open experiment.SETTING: Department of Pathology, Tianjin Medical College of Chinese People's Armed Police Force; Institute of Endocrinology of Tianjin Medical University.MATERIALS: This study was carried out in the Tianjin Medical University between January 2003 and March 2005.Ten-to-twelve-week-old aborted fetuses were obtained from the General Hospital of Tianjin Medical University with the approval of the local ethical committee. Informed consents were obtained from the mothers and their relatives.METHODS: ①Under the aseptic condition, the bilateral cortex of human fetal brain was removed and dissociated by brief mechanical trituration in D-Hanks. Then, 20 μg/L bFGF and 30 nmol/L T3 were used to induce the proliferation of NSCs and inoculated to poly-L-lysine-coated 24-well plate and 25 mL culture flask for routine culture at 1 ×109 L-1. The culture medium was DMEM/F12 serum-free complemented with N2. Half of the culture medium was changed every 48 hours.Seven days later, bFGF was discarded, only T3 was used for induction and differentiation. ② At 1, 2 and 3 weeks of culture, cells were collected, and RT-PCR was semiquantitatively used to detect TR mRNA expression changes at different stages of differentiation of NSCs. Isoforms were identified by immuocytochemistry.MAIN OUTCOME MEASURES: ①Cellular morphology observation and isoforms identification before and after differentiation of NSCs induced by T3. ② TR mRNA expression changes during the differentiation of NSCs.RESULTS: ①The hNSCs were round and had a smooth surface and gradually gathered to neurospheres. The proliferative hNSCs were nestin-positive and incorporated BrdU. When NSCs were induced by T3 for one week, most of the cells took on monopole or double poles, and had long and thin processes. The differentiated cells were neurofilament protein (NFP)-positive, galactocerebroside (GC)-positive or glial fibrillary acidic protein (GFAP)-positive. When NSCs were induced by T3 for three weeks, most of the cells were big, with unclear cell membrane, round nucleus, many thick processes which had many branches. The spider-like cells were scattered, and 80% of the cells were myelin basic protein-positive. ② TRα1 mRNA expression level was the highest before inducing NSCs. With the induction of T3, the expression level was decreased gradually, and was the lowest at 2 weeks, and then was rebounded gradually, but the final level was still lower than that of NSC (F =32.49, P =0.008). The tendency of TRα2 mRNA expression alteration was identical with that of TRα1 mRNA. TRβ1 mRNA expression level was the lowest in NSC, was increased gradually with the induction of T3 and attained the highest level at 2 weeks of induction of T3. Furthermore, the expression level of TRβ1 mRNA was also higher than that of TRα1 at the same time (t =15.64,P =0.001), and it reached the lowest level at 3 weeks of the induction. TRα3 expression level was firstly decreased after the differentiation induced by T3, and was close to the expression level of NSC at 2 weeks of induction (F =51.94, P =0.378), then was decreased to lower lever.CONCLUSION: T3 can induce NSC to differentiate into neurons, oligodendrocyte and astrocytes. TR mRNAs are expressed in different time intervals during the differentiation of NSCs.