1.An Evaluation on Bilingual Teaching in Biochemistry
Chinese Journal of Medical Education Research 2006;0(11):-
A questionaire before class,an analysis and an interview after exam were applied to evaluate the effect of bilingual teaching in biochemistry to provide important experiences and directions for carrying out bibingual teaching in the future.
2.Practice and Construction of the Quality Course of Biochemistry
Li JIANG ; Wenli MA ; Jin LI ; Shiliang CHEN ; Lina ZHU ; Chunqiong FENG
Chinese Journal of Medical Education Research 2006;0(11):-
Biochemistry is one of the major courses in basic medical science.All through the construction of the course in recent years,several teaching reforms have been taken in optimizing the content,improving teaching methods,enhancing education technique and intensifying teaching group,which contributed a lot to good teaching effect.As a result,the course of biochemistry was appraised as State-class Quality Course in 2005.
3.DETECTION OF SPERMATOZOAL TOTAL RNAS BY LABORATORY ON CHIP GEL ELECTROPHORESIS
Xiangming MAO ; Wenli MA ; Chunqiong FENG ; Yanbin SONG ; Rong SHI ; Qiulin XU ; Yaguang ZOU ; Li JIANG ; Wenlin ZHENG
Acta Anatomica Sinica 1955;0(03):-
Objective Detecting of spermatozoal total RNAs by laboratory on chip gel electrophoresis so that it could provide better total RNAs for the sequent experiments, and spur the development of spermatozoal molecular biology. Methods Sperms of healthy adults were collected and then total RNAs were extracted by RNeasy mini kit(QIAGEN), detection and quality control were performed by loboratory on chip gel electrophoresis system. Meantime, the control RNAs were extracted from lymphocytes. Results It was found that there were a plenty of genes expressed in healthy sperms. Electrophoretic graphs showed that the total RNAs of spermatozoal had 2 bands which went ahead a little comparing to the normal somatic cells. The former peak appeared keenness, and the latter was broad and showed like a reversed U. The ratio of them was largely more than 2, no extra peaks were found in electrophoretic graph. Conclusion A simple,intuitionistic method to detect and control the quality of the healthy adults' spermatozoal total RNAs had been successfully constructed by using laboratory on chip gel electrophorosis.
4.Differentiation between hyperintense hepatocellular carcinoma and focal nodular hyperplasia in hepatobiliary phase with multimodal parameters of magnetic resonance imaging
Ye LANG ; Chunqiong MA ; Liwei DENG ; Jian WANG ; Chen LIU ; Shilei XIAO ; Jiang DENG ; Qian LI ; Wenwen LIU ; Desheng YANG ; Xiaoming LI
Chinese Journal of Hepatology 2022;30(10):1069-1073
Objective:To differentiate hyperintense hepatocellular carcinoma (HCC) with focal nodular hyperplasia (FNH) in the hepatobiliary phase by MRI multimodal parameters.Methods:A retrospective cross-sectional study method was adopted. Clinical data on 15 cases with hyperintense HCC and 15 cases with FNH in the hepatobiliary phase admitted to the First Affiliated Hospital of the Army Medical University from January 2012 to December 2019 were collected. All patients with solitary lesions who underwent Gd-EOB-DTPA-enhanced MRI examinations were included. Surgically resected specimens were verified by pathological and immunohistochemical examination. HCC and FNH imaging features were analyzed by two radiologists.Results:(1) HCC and FNH apparent diffusion coefficient (ADC) values were 1 205.07±239.65×10 -3 mm 2/s and 1 434.73±217.6×10 -3 mm 2/s, respectively, and the SI ADC difference was statistically significant ( P<0.05) between the two groups. (2) In the dynamic contrast-enhanced MRI sequence, 15 cases of HCC were significantly enhanced in the arterial phase, of which 13 cases were characterized by continuous enhancement, and 2 cases were characterized by wash-in and wash-out enhancement. There was no statistically significant difference ( P>0.05) between the two groups. SI enhancement rate between HCC and FNH (1.39±0.60 vs. 1.33±0.50, P>0.05) had no significant difference. (3) HCC and FNH morphological features in the hepatobiliary phase included: annular hypointensity: HCC (8 cases) vs. FNH (0 cases); contrast filling defects: HCC (8 cases) vs. FNH (0 cases); linear hyposignal separation: HCC (10 cases) vs. FNH (0 cases); and stellate scars: HCC (0) vs. FNH (5 cases), and there were statistically significant differences ( P<0.05) between the two groups . Conclusion:Multimodal MRI have significant value for differentiating hyperintense HCC and FNH in the hepatobiliary phase.