Objective To reveal the epidemiological characterization of brucellosis in Shanxi province in recent years.Methods Retrospective investigation was made from the 105 diagnosed brucellosis cases in the first hospital affiliated to Shanxi medical university during 2013.1 ~2015.3.Results Peasants accounted for 89.52%and urban residents was 10.48% in 105 cases;male was the majority.Most cases were seen in late summer and early autumn.88.57% of the cases had clear history of epidemiology,and only 16.85% of those who got close contact with livestock took uncomplicated preventive measures.Conclusion Transmission mode of brucellosis has greatly changed in Shanxi province in recent years.Infected cases have increased year by year,and incidence ratio in urban residents is rising.Extensive breakout is replacing by fragmented scattering point.

OBJECTIVE To characterize the relationship between promoter of FGL2 gene(site-1285T/A)polymorphism and clinical subgroups infected with HBV. METHODS The genotype was analyzed by using method of polymerase chain reaction(PCR),enzyme restriction after PCR amplification,and agarose gel electrophoresis.Statistics were used ?~2 and Logistic regression. RESULTS There were no statistic genotype or allele frequency differences between any two subgroups(except acute hepatitis B and liver cancer). CONCLUSIONS Promoter of fgl2 gene(site1285T/A) polymorphism has no relation with HBV infection.

High mobility group protein B1 (HMGB1) is encoded by HMGB1 gene and is a highly conserved non-histone chromosomal binding protein widely present in the nucleus. It plays an important role in DNA replication and repair of eukaryotic cells. As a delayed inflammatory mediator, HMGB1 secreted or released extracellularly mediates inflammatory response in sepsis, autoimmune diseases, malignant tumors, liver-related diseases, and so on. In recent years, the studies of HMGB1 gene polymorphism have provided a theoretical basis for further clarifying the pathogenesis of diseases. These studies provided early predictions of disease susceptibility for early intervention and provided the basis for precise prevention and treatment of diseases. In this paper, the correlation between HMGB1 and clinical disease susceptibility and prognosis was reviewed.

Objective: To analyze the clinical epidemiological situation and trend of brucellosis in Shanxi Province in order to provide the basis for diagnosis. Methods: Brucellosis cases admitted to the First Hospital of Shanxi Medical University during January 2013 to December 2015 were included in the study. A retrospective investigation was undertaken and patient files were reviewed for general situation, clinical and laboratory findings, the process of diagnosis and treatments. Results: The data of 141 patients were collected, including 100 males and 41 females, the male to female ratio was 2.44 ∶ 1.00; the majority were 51-60 years old, 49 cases, accounting for 34.75%; the peak incidence was July and August each year, a total of 51 cases, accounting for 36.17%; of the 141 patients, 112 (79.43%) were professional, 29 (20.57%) were non-professional; and the clinical performance was diverse, 114 (80.85%) patients with irregular heat type, only 17 cases (12.06%) showed typical migratory joint pain; 31 cases (21.99%) were first diagnosed with only brucellosis, the shortest misdiagnosis time was 1 week, and the longest 18 month, the average misdiagnosis time was 3 months, and the total misdiagnosis rate was as high as 78.01% (110/141). Conclusions Significant changes have happened in the clinical epidemiological characteristics of brucellosis in Shanxi Province. Various forms of prevention knowledge propaganda and education should be continually carried out, so as to strengthen the awareness and attention of the clinical workers on the disease.

Japanese encephalitis virus (JEV) is the leading cause of viral encephalitis in Asia and domestic pigs serve as the amplifying hosts. In the present study, the full genomic sequences of two JEV strains (HEN0701 and SH0601) isolated from pigs in China were determined and compared with other 12 JEV strains deposited in GenBank. These two strains had an 88.8% nucleotide sequence similarity and 97.9% deduced amino acid sequence homology. HEN0701 had high nucleotide sequence and high amino acid sequence identity with genotype I (GI) strains, while SH0601 had high nucleotide sequence and high amino acid sequence identity with GIII strains at both the gene and full genome levels. Further phylogenetic analysis showed that HEN0701 belonged to the JEV GI group and SH0601 was classified as a GIII strain. Analysis of codon usage showed there were a few differences between the GI and GIII strains in nucleotide composition and codon usage for the open reading frames.
Animals ; Cell Line ; Cricetinae ; Encephalitis Virus, Japanese/classification/*genetics ; Encephalitis, Japanese/epidemiology/*veterinary/virology ; Gene Expression Regulation, Viral/physiology ; Genome, Viral ; Molecular Epidemiology ; Phylogeny ; Swine ; Swine Diseases/epidemiology/*virology

Japanese encephalitis virus (JEV) is the leading cause of viral encephalitis in Asia and domestic pigs serve as the amplifying hosts. In the present study, the full genomic sequences of two JEV strains (HEN0701 and SH0601) isolated from pigs in China were determined and compared with other 12 JEV strains deposited in GenBank. These two strains had an 88.8% nucleotide sequence similarity and 97.9% deduced amino acid sequence homology. HEN0701 had high nucleotide sequence and high amino acid sequence identity with genotype I (GI) strains, while SH0601 had high nucleotide sequence and high amino acid sequence identity with GIII strains at both the gene and full genome levels. Further phylogenetic analysis showed that HEN0701 belonged to the JEV GI group and SH0601 was classified as a GIII strain. Analysis of codon usage showed there were a few differences between the GI and GIII strains in nucleotide composition and codon usage for the open reading frames.
Animals ; Cell Line ; Cricetinae ; Encephalitis Virus, Japanese/classification/*genetics ; Encephalitis, Japanese/epidemiology/*veterinary/virology ; Gene Expression Regulation, Viral/physiology ; Genome, Viral ; Molecular Epidemiology ; Phylogeny ; Swine ; Swine Diseases/epidemiology/*virology

The corona-like spikes or peplomers on the surface of the virion under electronic microscope are the most striking features of coronaviruses. The S (spike) protein is the largest structural protein, with 1,255 amino acids, in the viral genome. Its structure can be divided into three regions: a long N-terminal region in the exterior, a characteristic transmembrane (TM) region, and a short C-terminus in the interior of a virion. We detected fifteen substitutions of nucleotides by comparisons with the seventeen published SARS-CoV genome sequences, eight (53.3%) of which are non-synonymous mutations leading to amino acid alternations with predicted physiochemical changes. The possible antigenic determinants of the S protein are predicted, and the result is confirmed by ELISA (enzyme-linked immunosorbent assay) with synthesized peptides. Another profound finding is that three disulfide bonds are defined at the C-terminus with the N-terminus of the E (envelope) protein, based on the typical sequence and positions, thus establishing the structural connection with these two important structural proteins, if confirmed. Phylogenetic analysis reveals several conserved regions that might be potent drug targets.
Amino Acid Sequence ; Antigens, Viral ; immunology ; Base Composition ; Computational Biology ; Enzyme-Linked Immunosorbent Assay ; Membrane Glycoproteins ; genetics ; Molecular Sequence Data ; Mutation ; genetics ; Phylogeny ; Protein Structure, Tertiary ; SARS Virus ; genetics ; immunology ; Sequence Analysis, DNA ; Sequence Homology ; Spike Glycoprotein, Coronavirus ; Viral Envelope Proteins ; genetics ; metabolism