Objective To evaluate the value of the peripheral capillary fasting plasma glucose examined by glucometer on screening of type 2 diabetes.Methods With the proportion stratified group sampling,all residents aged 15-75 years in Wuqing district were selected and detected on their capillary fasting plasma glucose(CFPG) by clucometer,and those whose CFPG was higher than 5.0mmol/L should have a 2-hour postprandial glucose(2 h PG) test.Data were analyzed by SPSS 11.5 software.Results The detection rates of diabetes,impaired glucose tolerance and impaired fasting blood glucose were 42.83%,27.88% and 3.74%,respectively.Making two ROC curve with test variable(FPG) and state variables(2 h PG≥11.1 mmol/L and 7.8 mmol/L≤2 h PG≤11.0 mmol/L),the areas under the curve were 0.862 and 0.405,respectively.Conclusion It is suggested to use the sensitivity standard that nears to 99% FPG≥5.0 mmol/L to screen diabetes.

Aim To establish a rat aortic calcification sub-chronic model induced by vitamin D3 and nicotine.Methods The vascular calcification model was produced by vitamin D3(300 kIU?kg-1,im) plus nicotine(25 mg?kg-1,5 ml?kg-1,po).Rat body weight and blood pressure were monitored on a regular basis.Ratio of heart to body weight,plasma calcium,plasma phosphate content,vascular calcium content,Von Kossa staining,activity of alkaline phosphatase(ALP) and 45Ca deposition in aorta were measured as the index of calcification at the 8th and the 16th week.Results Compared with control group,the VDN group rats showed higher values of blood pressure from the 3rd week(all P

Objective To discuss the role of interleukin (IL)-12 in capillary bronchiolitis disease.Methods Fifty-nine cases of capillary bronchiolitis children under 2 years old were enrolled as the bronchiolitis group Ⅰ(n =28) and bronchiolitis group Ⅱ(n =31),36 cases of children with bronchopneumonia of the same age and 31 cases of children suffered from non-infectious diseases such as hernia and renal calculus of the same age were enrolled as bronchopneumonia control group and normal control group respectively.The peripheral blood IL-12 levels of four groups were detected by enzyme-linked immunosorbent assay.Results The levels of the peripheral blood IL-12 in bronchiolitis group Ⅰ,bronchiolitis group Ⅱ,bronchopneumonia control group and normal control group were (34.72±7.96) pg/ml,(55.30 ±6.72) pg/ml,(56.79±10.36) pg/ml and (61.23 ± 11.51) pg/ml respectively.The level of the peripheral blood IL-12 in bronchiolitis group I was significantly lower than that in bronchiolitis group Ⅱ,bronchopneumonia control group and normal control group respectively (P ＜0.05),and the level of the peripheral blood IL-12 in bronchiolitis group ⅡⅡ was significantly lower than that in bronchopneumonia control group and normal control group respectively (P ＜ 0.05),but there was no significant difference between bronchopneumonia control group and normal control group (P ＞ 0.05).Conclusion The level of IL-12 is one of the important factors for bronchiolitis disease,the reducing of serum level of IL-12 in children who have a high risk factor of capillary bronchiolitis is more obvious.

Objective To observe the effect of aerobic exercise of different intensities on type Ⅱ collagen,glycosaminoglycan (GAG) and chondrocyte apoptosis in rabbits modeling knee osteoarthritis (OA),so as to explore the preventive effect and its possible mechanism.Methods Twenty healthy New Zealand white rabbits were randomly divided into Groups A,B,C and D,each of 5.Group A was allowed free activity in a cage for 9 weeks.Group B was allowed free activity for 4 weeks,then an OA model was established using papain and confirmed via MRI 1 week later,Another 4 weeks of free activity were then allowed.Groups C and group D were given running training for 20 minutes a day at 0.5 km/h,3 times a week,and then 20 minutes a day at 1.5 km/h,5 days a week on a treadmill for 4 weeks.Nine weeks later,all 4 groups of rabbits were killed and the articular cartilage damage of each group was compared using Mankin scoring,and expression of type Ⅱ collagen,GAG content and chondrocyte apoptosis in the cartilage.Results After the intervention,the average Mankin score,expression of type Ⅱ collagen and GAG content of groups B,C and D were significantly lower than those of group A,and all of those values in group B were significantly lower than those of group D.After 9 weeks the chondrocyte apoptosis rate of group A was significantly lower than that of the other groups,and that of groups C and D was significantly lower than that of group B.Conclusion Aerobic exercise may prevent knee articular cartilage degeneration through inhibiting reduction in the amount of type Ⅱ collagen and GAG in the cartilage matrix.It may be related to decreasing the chondrocyte apoptosis.

Background:Chronic atrophic gastritis(CAG)is a kind of chronic gastritis with atrophic changes of gastric mucosa. The studies on peripheral blood biomarkers in CAG are rare. Aims:To investigate the methylation of peripheral blood CpG sites in Runx3 gene promoter region in CAG patients. Methods:Eighty-two mild CAG patients,73 moderate to severe CAG patients from June 2013 to May 2014 at Daqing Oilfield General Hospital were enrolled,and 45 patients with normal gastric mucosa were served as controls. The methylation of CpG sites in Runx3 gene promoter region was measured by MALDI-TOF-MS. mRNA expression of Runx3 was determined by fluorescent quantitative PCR,and the protein expression of Runx3 was determined by Western blotting. Results:Compared with the control group and mild CAG group,methylation levels of CpG13,CpG14 and CpG15 sites in Runx3 gene promoter region were significantly increased in moderate to severe CAG group(P < 0. 05),the mRNA and protein expressions of Runx3 were significantly decreased( P < 0. 05). No significant differences in methylation of CpG sites in Runx3 gene promoter region and mRNA and protein expressions of Runx3 were found between mild CAG group and control group( P >0. 05 ). Conclusions:The hypermethylation of peripheral blood CpG13,CpG14 and CpG15 sites in Runx3 gene promoter region can inhibit the expression of Runx3 in CAG patients,and can be used potentially as the biomarker for clinical staging of CAG.

A multi-stage stratified sampling method and a self-designed questionnaire were used to evaluate the prevalence of conjunctivitis symptoms and associated factors among the Beijing residents.In total,12 890 residents aged over 18 years were recruited from 6 districts.The reported prevalence of conjunctivitis symptom at the last years was 1.60％.Self-reported health condition and personal health behaviors were risk factors associated with conjunctivitis among adult Beijing residents.No significant difference existed between different districts,age groups,educational levels,gender and races.Conjunctivitis symptom among Beijing residents was related with health condition and health behaviors.More measures should be taken to cultivate the health model of behaviors.

Objective To study the sensitivity and specificity of the Chinese eating assessment tool (EAT-10) in screening acute stroke patients for oropharyngeal dysphagia (OD).Methods A total of 130 inpatients with acute stroke were screened using the Chinese EAT-10.On the same day they were also screened using the gold standard technique for diagnosing dysphasia-videofluoroscopy.A receiver operating characteristics (ROC) curve was developed to study EAT-10's sensitivity and specificity.A Youden index,positive predictive value (PPV),negative predictive value (NPV),and positive and negative likelihood ratios (LHR+ and LHR) were quantified.Results According to the ROC curve,a cut-off point of 1 (EAT-10 score≥ 1) gave the best sensitivity (77.9％),the highest NPV (73.2％),with 66.1％ specificity,71.6％ PPV,2.30 LHR+ and 0.33 LHR in screening for OD.The test-retest reliability was above 0.7.An investigator consistency reliability test showed good repeatability,and the consistency between each item and the mean total score was high.Conclusion The Chinese EAT-10 has good test-retest reliability and investigator consistency.The optimal cut-off point is 1,with good sensitivity and NPV at scores ≥ 1.The test can be recommended as a screening tool for OD in acute stroke patients.

To assess the level of health skills relating to infectious diseases among residents in Beijing and develop more effective strategies for controlling infectious diseases.We conducted a multistage sampling,retrospective cross-sectional survey of residents aged 18 and above from 6 districts by selfadministered and anonymous questionnaires in January 2011.The questionnaires consisted of health skills relating to infectious diseases.A total of 13 042 respondents completed the survey.The proportions of people with the skills for acquiring knowledge about infectious diseases,being able to read medicinal package insert,being able to read brochures with regard to basic knowledge about infectious diseases, being able to read laboratory test report andproper use of thermometer were 57.7％,71.0％,70.2％,47.9％ and 76.8％ respectively.The levels of health skills were different among different populations (P ＜0.01).A low level of health skills was observed among the poorly-educated people and elders.Therefore measures such as health education and skills training about infectious diseases should be taken to improve the health skills.

Objective:To study the influence of rutin in the morphology of renal tissue of the diabetic mice induced by streptozotocin(STZ), and to clarify the effect of rutin on the kidney tissue damage.Methods:Twelve mice of the total 70 Kunming mice were used as normal group, the other were used to estabish type 1 diabetes mouse models by intraperitoneally injected with STZ (62.5 mg·kg-1), once daily for 5 d.The successfully established model mice were randomly divided into model group,low dose (50 mg·kg-1)of rutin group, high dose (100 mg·kg-1) of rutin group and irbesartan group (45 mg·kg-1).The mice in model group and normal group were given the carboxy methyl cellulose(CMC) and the other mice were given drugs by intragastric administration once daily for 8 weeks accordingly.The weight and blood glucose of the each mouse were determined.Full automatic biochemical analyzer was used to detect the levels of serum creatinine (Cre) and blood urine nitrogen (BUN) of the mice , and the kidney index was calculated.The morphology of renal tissue was observed by HE staining, Masson staining and electron microscope.Results:After injection of STZ,the model success rate was up to 98%.Compared with normal group, there was no significant difference in the weight of the mice in other groups before administration(P>0.05).After administration of rutin, the weights of the mice in model group, low dose of rutin group, high dose of rutin group and irbesartan group were significantly decreased (P<0.05).Compared with model group, the levels of blood glucose of the mice in low and high doses of rutin groups were significantly decreased(P<0.05);the levels of Cre and BUN were significantly reduced (P<0.05);the kidney index of the mice in high dose of rutin group was significantly decreased (P<0.05).Compared with normal group,the kidney tissue of the mice in model group was seriously damaged;glomerular was weaked, the kidney tissue fibrosis was serious, glomerular basement membrane was diffusely thickened and foot process was coalesced or overgrow.Compared with model group,the degree of injury of the mice in low and high doses of rutin groups were significantly improved, especially in high dose of rutin group.Conclusion:Rutin can improve the renal function of diabetic mice induced by STZ and reduce the degree of renal tissue damage in the diabetic mice

Objective To explore the clinical features,efficacy and prognosis of different treatments for children with achalasia of cardia(AC).Methods In this retrospective study,the clinical features,laboratory examination and treatment of 36 children with AC who had been admitted to Department of Gastroenterology,Beijing Children's Hospital,Capital Medical University from August 2006 to September 2015 were reviewed,and the efficacy and prognosis of different treatments were compared.The symptoms of the children were graded using the AC clinical symptom score(Eckardt score),and the Eckardt score ≤ 3 scores was defined as the effective treatment.SPSS 19.0 statistical software was used to analyze the data,and P<0.05 for the difference was statistically significant.Results Thirty-six children with AC included 24 boys and 12 girls.Ages ranged from 1.4 to 15.5 years old,with a mean age of(10.0±3.4)years old.Course of disease ranged from 1 month to 9 years,with a mean course of 0.5(0.2,3.0)years.In the 36 children,33 cases(91.7％)had vomiting,23 cases(63.9％)had dysphagia,16 cases(44.4％)had weight loss,and 9 cases(25.0％)had chest pain.The effective rates of treatment in surgical treatment group and drug treatment group were 100.0％(13/13 cases)and 71.4％(5/7 cases),respectively in 3 months,and there was no significant difference between the 2 groups(P=0.111).The effective rates of treatment were 100.0％(13/13 cases)and 50.0％(3/6 cases),respectively in 6 months,and the difference was statistically significant between the 2 groups(P=0.021).Within 12 months,there was no recurrence in surgical treatment group and the effective rate was 100.0％.Children in drug treatment group had 1 case who stopped taking medicine,while the other children received surgical treatment in other hospitals due to poor drug treatment.Conclusions Drug and surgical treatment of AC both have good short-term effect,however,the medium and long-term efficacy of surgical treatment is higher than that of drug treatment in children.Symptomatic relief is more stable,and symptom is not easy to relapse for the children with surgical treatment.