1.SUDIES ON THE CHEMICAL CONSTITUENTS OF THE DAYAWAN BAY GERM PSEUDOMONAS SP.
Chunming ZENG ; Guoqiang LIN ; Haoqin SHEN
Chinese Journal of Marine Drugs 1994;0(02):-
A red pigmant, procligiosin, have been isolated from the metabolite of germ pseudomonas sp. collected from the Dayawan Bay. Its structure was established on the basis of the spectral method.
2.The management of gallbladder polypoid lesions
Xingwu YANG ; Tao LIN ; Chunming YANG
Chinese Journal of Hepatobiliary Surgery 2012;18(10):733-735
ObjectiveTo discuss the diagnosis method and management of Gallbladder polypoeid lesions (GPL).MethodA ten-year (2002-2011) retrospective reviews of all patients result underwent in ultrosenography (US) was conducted.ResultsTotal 591 peatients with GPL detected on US were identified,349 patients (59.1%) were women,the age ranges 20 to 93 years.395 patients had abdominal symtoms,of which 39.9% patients had a single polyp and 9.8% had gallstones.The specifisity of the diagnosis of GPL in US was 92.5 %.Laparascopic cholecystectomy (LC) is the treatment of 136 chosen cases.In cases suspicious of malignancy,it is advisable to do cholecystectomy.ConclusionsMost small GPL are benign and remain static for years.Age more than 50 years old and size of polyp more than 10 mm are the two main important risk factors predicting malignancy in GPL.Other risk factors include concurrent gallstones,solitary polyp,and symptomatic polyp.LC is the treatment of unless since the suspicion of malignancy is high.
3.Characteristics and prognostic analysis of breast cancer in young women
Tao LIN ; Xingwu YANG ; Chunming YANG
Journal of Endocrine Surgery 2012;06(5):327-329
Objective To investigate the clinical features,surgical treatment and prognosis of female breast cancer patients under 35 years.MethodsThe clinical data of 140 cases of female breast cancer patients in the young group ( ≤ 35 years) and 326 cases of female breast cancer patients in the control group ( 45 - 59years) undergoing surgical treatment from 2002 to 2009 in our hospital were analyzed retrospectively.All the data were analyzed with the statistical software of SPSS13.0.ResultsThe difference between the 2 groups had no statistical significance in tumor size or pathologic types( P >0.05).The axillary lymph node metastasis rate was obviously higher in the young group than in the control group ( P < 0.05 ). Modified radical mastectomy and breast-conserving surgery were mainly performed.The proportion of patients in stage Ⅲ and Ⅳ in the young group was higher than that in the control group.The positive rate of ER and PR was lower while the positive rate of HER-2 was higher in the young group than those in the control group( P < 0.05 ).ConclusionsBreast cancer in young women has the features of high clinical stage,high lymph node metastasis rate,low positive rate of ER and PR,high positive rate of HER-2,high recurrence rate and poor prognosis.The treatment principle of the diseaseis comprehensive therapies mainly based on surgical treatment.
4.Diagnosis and treatment of thyroid nodule: standard discussion
Kai HUANG ; Tao LIN ; Xingwu YANG ; Chunming YANG
Journal of Endocrine Surgery 2011;05(2):95-98
Objective To discuss the standard diagnosis and treatment for thyroid nodule.Methods 2 581 patients with thyroid nodule from 2005 to 2009 were studied.Common methods of examination and treatment were analyzed to avoid over-examination and over-treatment.Results 90%of the patients underwent ultrasound examination with a 92.9%sensitivity and 44.3%specificity when there were two or more risk characters.All patients underwent certain kinds of surgery according to their clinical conditions.The recurrence rate was 4%two and half years after lateral thyroid adenectomy or thyroid surgery without excision of thyroid isthmus.Conclusions There are many approaches to thyroid nodule examination and treatment,in which ultrasound is recommended for preoperative examination because of its higher sensitivity,specificity and lower cost,and surgery is still the most useful and effective treatment.Choosing the proper method for thyroid nodule diagnosis and treatment is the key point to avoid over-examination and over-treatment.
5.The effects of RNA interference on the different fragments of osteopontin expression in hepatocellular carcinoma
Fan LIN ; Weili GU ; Shaofeng FAN ; Kunping LI ; Chunming LIN ; Zhenghao PEI
Chinese Journal of Hepatobiliary Surgery 2012;18(4):288-291
Objective Within human hepatoma cell lines,we aimed to investigate the effects of the down-regulation by RNAi on different fragments of osteopontin (OPN) in order to discover more effective and accurate sites for OPN.Methods Specific small interfering RNA of OPN (OPNi-1) were synthesized and transfected into human hepatoma cell line (HEP-G2).Fluorescent quantitative PCR and immunohistochemical methods were used to test*the OPN expression levels of mRNA and protein before and after RNAi.Results After transfection,the △CT value of the A fragment was greater than B and C fragments of OPN mRNA in HEP-G2.Before RNAi was added to HEP-G2 cells,the three fragments A,B,C had OPN mRNA CT values of 8.31±1.58,8.78±1.49,8.25±1.51 respectively.Once the RNAi were added,the CT values were measured 48h after for the fragments A,B,and C which were 12.14±1.43,10.22±1.97,10.48±1.88 (P<0.05) respectively.The immunohis tochemical values of A,B,C were down from 6.44±1.67,5.43±2.05,5.45±2.52 to 2.84±1.52,4.43± 1.65,3.95± 1.43 respectively after interference.Conclusions RNAi can inhibit the expression of OPN gene selectively.siRNA targets different segments of OPN,which may have more effects on invasion and metastasis of liver cancer for a more important significance in science and health economics.
6.The correlation of serum SOD and CD4+CD25+regulatory T cell in the mechanism of severe sepsis
Junling LI ; Hancong WU ; Tao DENG ; Yixian LIANG ; Chunming LIN ; Wenlong DENG ; Jie ZHANG ; Manni QIN
The Journal of Practical Medicine 2017;33(15):2536-2538
Objective To evaluate the condition of oxidative stress and immunosuppression in early stage of severe sepsis,and investigate the correlation between them. Methods A prospective random control study in-cluded patients group(n=51)and control group(n=31). The concentration of serum superoxide dismutase was measured by enzyme linked immunosorbent assay(ELISA),CD4+CD25+Treg% was measured by flow cytometry , respectively. The difference between two groups was compared and the correlation between parameters in patients group was evaluated. Results The concentration of serum SOD was lower than control group (P < 0.01). CD4+CD25+Treg% significantly high,compared to the control group(P < 0.01). There was no strong correlation be-tween parameters in patients group. Conclusion Oxidative stress and immunosuppression are exist in the early stage of severe sepsis.
7.The Challenge and Countermeasure of Pharmacy Automation Construction in Public Hospital
Chunming WANG ; Weiping LI ; Xianming KONG ; Huili DAI ; Na GENG ; Houwen LIN ; Jie SHEN ; Guohong LU
China Pharmacy 2015;(34):4810-4812,4813
OBJECTIVE:To demonstrate challenges of pharmacy automation reconstruction so as to set solutions. METHODS:Based on literature review,analysis of pharmacy automation setting and features,this paper gave the suggestions and solutions on construction cost,management model change,equipment maintenance and emergency response,etc. according to the practice of the hospital. RESULTS&CONCLUSIONS:Pharmacy automation construction should be stick to the requirements of new health re-form to lower the cost by using the out resources and interior optimal allocation,to improve efficiency by unified planning and proper design,and to ensure the system running efficiently by sufficient maintenance and contingency plan.
8.The expression of livin of kidney tissues in rats with acute kidney injury induced by endotoxin
Lin TONG ; Biao ZHANG ; Huaguo YAO ; Xiaocong SUN ; Ji CHEN ; Chunming GUAN
Chongqing Medicine 2014;(7):826-827
Objective To investigate the expression of livin of kidney tissues in rats with acute kidney injury (AKI) induced by endotoxin ,and the role of livin in the cell apoptosis of AKI .Methods The rat models with AKI were induced by endotoxin .The de-gree of kidney injury was observed by hematoxylin and eosin stain ,measuring the levels of the serum creatinine and urea nitrogen . The expression of livin and caspase-3 in kidney tissue at different time points was analyzed by immunohistochemistry assay ,and the relationship between the expression of livin and caspase-3 and kidney injury was analyzed .Results Compared to the control group , the rats injected endotoxin had the performance of AKI ,with obviously pathomorphological damage in the kidney tissues ,signifi-cantly increasing in the serum creatinine and urea nitrogen (P<0 .01) .The livin and caspase-3 of kidney tissues in rats with AKI caused by endotoxin were positive expression (P<0 .01) .With the lapse of time ,the trend of increasing of caspase-3 showed gradu-ally slow after the highest expression of livin .Conclusion The livin involved in the pathogenesis of endotoxin-induced AKI ,it might relieve the kidney injury and protect renal function by inhibiting casepase-3 important apoptotic effector protein .
9.Influences of multiple gene interactions on bone mineral density and osteoporotic fractures in postmenopausal women
Hanxiao SUN ; Lin ZHAO ; Minjia ZHANG ; Yanhua DENG ; Bin CUI ; Shuangxia ZHAO ; Chunming PAN ; Bei TAO ; Lihao SUN ; Hongyan ZHAO ; Huaidong SONG ; Weiqing WANG ; Guang NING ; Jianmin LIU
Chinese Journal of Endocrinology and Metabolism 2012;28(8):641-646
Objective To investigate the effects of the genetic polymorphisms in osteoporosis-related genes and the gene-gene interaction on bone mineral density (BMD) and osteoporotic fractures.Methods Thirty-nine single nucleotide polymorphism (SNP) sites in 23 genes that related to bone mineral density ( BMD ) and osteoporotic fractures were scanned in 683 Shanghai Han postmenopausal women.TaqMan SNP Genotyping Assay or Sequenom Mass ARRAY System were applied for genotyping analysis.The relation of these SNP sites with BMD and osteoporotic fractures were analyzed.Results Altogether,12 SNPs in 9 candidate genes ( rs7524102 and rs6696981 in ZBTB40 gene,rs9479055 in ESR1 gene,rs6993813,rs6469804,and rs11995824 in OPG gene,rs3736228 in LRP5 gene,rs1107748 in SOST gene,rs87938 in CTNNB1 gene,rs1366594 in MEF2C gene,rs7117858 in SOX6 gene,and rs10048146 in FOXL1 gene) were associated with BMD at lumbar spine(L1-L4) or total hip.In addition,rs11898505 in SPTBN1 gene was related to osteoporotic fractures ( OR 0.522,95% CI 0.326-0.838,P =0.007 ).Gene-gene interaction involving rs1038304 in ESR1 gene,rs1366594 in MEF2C gene,and rs10048146 in FOXL1 gene was associated with osteoporotic fractures ( P =0.010 7 ).Conclusions ( 1 ) SNPs in gene ZBTB40,ESR1,OPG,LRP5,SOST,CTNNB1,MEF2C,SOX6,FOXL1,and SPTBN1 are associated with BMD of lumbar spine or total hip,as well as osteoporotic fractures.(2) Gene-gene interaction involving rs1038304,rs1366594,and rs10048146may contribute to the risk of osteoporotic fractures.
10.Study on the effect and mechanism of SKA1 activated by E2F1 transcription in promoting the proliferation of liver cancer cells
Xiaodan YE ; Linhong SU ; Jun LIN ; Jian CHEN ; Xiaping LIU ; Chunming WU ; Xiaoqu ZHU
Chinese Journal of Endocrine Surgery 2021;15(3):309-313
Objective:To investigate whether SKA1 is a key molecule regulating malignant proliferation of liver cancer, and further explore its mechanism to provide molecular theoretical basis for subsequent targeted therapy.Methods:The data of liver cancer from TCGA database were analyzed by bioinformatics technology. The expression of SKA1 in liver cancer was analyzed. At the same time, we also analyzed the relationship between the expression of SKA1 and the prognosis of patients with liver cancer. The hepatoma cell line overexpressing SKA1 was constructed by liposome-mediated cell transfection technique, and the effect of SKA1 on the proliferation of hepatoma cells was further tested by CCK-8 and plate cloning assay. At the same time, we found that E2F1 is also highly expressed in liver cancer, using bioinformatics technology to analyze the correlation between SKA1 and E2F1 expression, further detecting the binding site of E2F1 in the SKA1 promoter region, and using dual luciferase technology to detect E2F1 against SKA1. Transcriptional activation.Results:KA1 was highly expressed in liver cancer tissues, and the overall survival rate of liver cancer patients with high SKA1 expression was 49.8%, lower than that of patients with low SKA1 expression, showing a negative correlation. E2F1 is also highly expressed in liver cancer tissues, and the survival time of patients with liver cancer with high E2F1 expression is significantly lower than that in the low expression group, which was negatively correlated with poor prognosis. SKA1 overexpression could increase the proliferation ability of liver cancer cells by nearly 50%. SKA1 is regulated by the E2F1 transcription factor, and the E2F1 transcription factor is combined with the SKA1 promoter to transcriptionally activate the expression of SKA1 in liver cancer cells.Conclusion:E2F1 transcriptional activation of SKA1 promotes proliferation of hepatoma cells, leading to poor prognosis in patients with liver cancer