Objective To observe the effects of JAZF1 (Juxtaposed with another zinc finger gene 1 ) overexpression on glucose and lipid metabolism in 3T3-L1 adipocytes. Methods The tissue distribution of JAZF1 in healthy C57BL/6J mice was detected by real-time quantitative PCR( RT-QPCR). Expression vector for JAZF1 gene was constructed and transfected into 3T3-L1 adipocytes. The mRNA levels of JAZF1, GLUT1, GLUT4, FAS, ACC, SREBP1, ATGL, and HSL implicated in glucose and lipid metabolism were determined by RT-QPCR; JAZF1 protein level was measured by Western blot. Intracelluar lipid accumulation were measured by oil red O staining method. Results In JAZF1-transfected adipocytes, JAZF1 mRNA and protein levels were significantly higher than control cells after 48 h. The mRNA level of HSL was increased significantly (P<0. 05) in JAZF1 transfection group compared with negative control and empty vector group, and the expressions of FAS, ACC, SREBP1 mRNA were decreased significantly(all P<0.01). However, the mRNA levels of ATGL, GLUT1, GLUT4 were not changed. Intracelluar lipid accumulation was decreased significantly (P<0.05 ) by oil red O staining and colorimetric in JAZF1 -transfected cells compared with negative control and empty vector group. Conclusions There was an extensive expression of JAZF1 in various tissues of C57BL/6J mice,indicating that JAZF1 might play a role in maintaining normal physiological function. These results show that overexpression of JAZF1 in 3T3-L1 cells can reduce lipid synthesis, increase lipolysis, and improve lipid accumulation. JAZF1 might provide a new potential therapeutic target for obesity and diabetes.

The effects of tumor suppressor in lung cancer-1(TSLC1)upregulation on gene expressions involved in glucose and lipid metabolism in Hepa1-6 cells were investigated.The results showed that TSLC1 overexpression decreased fatty acid synthase and acetyl CoA carboxylase expressions(P<0.05 or P<0.01),increased adipose triglyceride lipase expression(P<0.05),and did not change hormone-sensitive lipase,glucose transporter (GLUT)1,and GLUT4 expressions.These results suggest that TSLC1 overexpression may promote lipolysis and inhibit adipogenesis in liver cells.

The effects of liraglutide on glucose and lipid metabolism, fibroblast growth factor-21 ( FGF-21 )and its receptors (FGFR) in APoE-/-mice with hypoadiponectinemia were investigated.Hypoadiponectinemia facilitated disturbance in glucose and lipid metabolism and insulin resistance. Compared with the control mice, FGF21 mRNA and protein expressions of liver and adipose tissues as well as plasma FGF-21 level were significantly increased in ApoE-/-mice with hypoadiponectinemia, along with lowered expressions of FGFR1 and β-klotho mRNA in adipose tissues, and expressions of FGFR1-3 and β-klotho mRNA in liver. Liraglutide administration improved glucose and lipid metabolism and insulin resistance, and partially reversed the changes of FGF-21 and its receptors induced by hypoadiponectinemia.

Objective:To explore the effect of pain threshold reduction nursing in patients with chronic non-healing wounds.Methods:From December 2017 to December 2020, convenience sampling was used to select 122 patients with chronic non-healing wounds in the China-Japan Friendship Hospital of Jilin University as the research object. According to the lottery method, the patients were divided into the control group and the observation group. The control group conducted conventional nursing, and the observation group carried out the pain threshold reduction nursing on the basis of the control group for a total of 2 weeks of nursing care. Wound edema, wound healing score, wound healing time, wound healing rate, Visual Analogue Scale (VAS) score were compared between the two groups of patients. The hospital stay and medical expenses of the two groups were compared.Results:The edema score at 48 hours after operation and wound healing score before discharge in the observation group were lower than those in the control group, and the difference was statistically significant ( P<0.01) . The wound healing time of the observation group was shorter than that of the control group, and the wound healing rate was higher than that of the control group, and the difference was statistically significant ( P<0.01) . The VAS scores of patients in the observation group before and after debridement and after nursing were lower than those of the control group, and the difference was statistically significant ( P<0.01) . The hospital stay and medical expenses of patients in the observation group were less than those in the control group, and the difference was statistically significant ( P<0.01) . Conclusions:Pain threshold reduction nursing can effectively improve the healing of patients' chronic wounds, promote wound repair, and increase the patient's tolerance to pain, further shorten the patient's hospital stay, and reduce the economic pressure of patients.

Objective:To explore the mediating effect of distress disclosure between perceived social support and psychological distress in colorectal cancer patients.Methods:The convenient sampling method was used to select 220 postoperative colorectal cancer patients who were reviewed in China-Japan Union Hospital of Jilin University from February to August 2023 as the research objetcs. The Distress Disclosure Index (DDI), Perceived Social Support Scale (PSSS), and Chinese version of Screening Tool for Psychological Distress in Cancer Patients were used to conduct the survey.Results:A total of 220 questionnaires were distributed in this study, and 213 valid questionnaires were recovered, with a valid recovery rate of 96.82%. The total score of the Chinese version of Screening Tool for Psychological Distress in Cancer Patients for colorectal cancer patients was (4.31±2.46), the total score of the DDI was (37.26±9.97), and the total score of the PSSS was (57.82±14.60). Pearson correlation analysis showed that psychological distress was negatively correlated with distress disclosure in colorectal patients ( P<0.01), distress disclosure was positively correlated with perceived social support ( P<0.01), and psychological distress was negatively correlated with perceived social support ( P<0.01). The mediating effect results showed that the direct effect of perceived social support on psychological distress was -0.359 ( P<0.01), and the mediating effect between perceived social support and psychological distress was -0.227 ( P<0.01), which acted as a partial mediating effect with the mediating effect accounting for 38.74% of the total effect. Conclusions:Perceived social support can not only directly affect the psychological distress of colorectal cancer patients, but also indirectly affect psychological distress through distress disclosure. Clinical staffs should take certain interventions to improve the level of patients' perceived social support and distress disclosure, so as to improve the psychological distress of patients.

Objective:To explore the direct impact of self-efficacy on the sense of personal mastery and the indirect impact mediated by uncertainty in illness in aged patients with chronic heart failure (CHF), and provide a theoretical basis for developing interventions to improve the sense of personal mastery in this patient population.Methods:Totally 240 aged CHF patients hospitalized in the Department of Geriatrics at the China-Japan Union Hospital of Jilin University from January to November 2023 were selected by convenience sampling. They were surveyed using the Personal Mastery Scale (PMS), the Mishel Uncertainty in Illness Scale (MUIS), and the General Self-Efficacy Scale (GSES). The Bootstrap method was employed to test the mediating effect of uncertainty in illness between self-efficacy and the sense of personal mastery.Results:A total of 240 questionnaires were distributed, with 221 valid responses collected, yielding a response rate of 92.08%. The total score for the PMS was (21.49±5.92), the total score for the MUIS was (75.00±20.94), and the total score for the GSES was (21.15±6.10). Mediating effect analysis showed that the total effect of self-efficacy on the sense of personal mastery was 0.557 (95% CI: 0.438-0.659; P=0.001) ; the direct effect was 0.311 (95% CI: 0.157-0.455; P=0.001) ; and the indirect effect through uncertainty in illness was 0.246 (95% CI: 0.169-0.344; P<0.001). Uncertainty in illness partially mediated the relationship between self-efficacy and the sense of personal mastery, accounting for 44.17% of the total effect. Conclusions:The sense of personal mastery in aged CHF patients is relatively low. Clinical healthcare providers should implement interventions to enhance patients' self-efficacy and reduce uncertainty in illness, thereby improving their sense of personal mastery.

Objective:To develop a Pediatric Nursing Care Deficiency Scale (PNCDS) and evaluate its reliability and validity.Methods:Based on theories of nursing care deficiency and considering pediatric nursing characteristics, the scale was developed following standard procedures and principles. This included semi-structured interviews, expert evaluations, a pilot survey, and a large-scale survey to finalize the scale.Results:The final version of the PNCDS comprised four dimensions with 25 items. The content validity index (CVI) of the overall scale was 0.937, with item-level CVIs ranging from 0.857 to 1.000. Exploratory factor analysis revealed that the factor loadings for all items were greater than 0.5, and the four factors accounted for a cumulative variance of 66.456%. Confirmatory factor analysis indicated a good model fit for the final scale. The overall Cronbach's α coefficient for the scale was 0.917, while the Cronbach's α coefficients for the four dimensions were 0.861, 0.923, 0.881, and 0.909, respectively.Conclusions:The PNCDS demonstrated good reliability and validity and provides a useful tool for assessing pediatric nursing care deficiencies and aiding in the development of intervention strategies.

Objective:To investigate the protective effect of ulinastatin on sepsis-acute kidney injury (SA-AKI) by NF-κB signaling pathway.Methods:Total of 60 mice were randomly(random number) divided into sham group, cecal ligation puncture group (CLP group) and ulinastatin treatment group (CLP+UTI group). Ulinastatin treatment group was intraperitoneally injected with ulinastatin 50 000 U/kg once a day. 24 hours after operation, five mice were sacrificed, the kidney tissues were collected to observe renal histopathology by HE staining. The macrophage infiltration was observed by immunohistochemistry. The remaining mice in each group were used to calculate the survival rate of 7-day after operation. HK-2 cells were stimulated by LPS to obtain the SA-AKI model, and the cells were divided into control group, LPS group and LPS + UTI group. CCK-8 assay was used to detect cell viability, EdU assay was used to detect cell proliferation, and JC-1 assay was used to detect mitochondrial damage. The phosphorylation degree of NF-κB was detected by western blot. Inflammatory factors concentrations of cellular supernatant were detected by ELISA assay.Results:Compared with the sham group, the kidney tissue of mice in CLP group showed that kidney pathological obvious changed, the infiltration of macrophages increased, and the survival rate of mice decreased. CLP+ UTI group reduced the pathological changes and the infiltration of macrophages, improved the survival rate of mice. Compared with control group, LPS group obviously inhibited the cells activity and proliferation of HK-2 cells, damaged the mitochondrial membrane potential of HK-2 cells. Compared with LPS group, LPS+ UTI group attenuated the phosphorylation of NF-κB, decreased the secretion of inflammatory factors, rescued the activity and proliferation of HK-2 cells, and reduced the damage of HK-2 mitochondrial membrane potential.Conclusions:Ulinastatin can reduce mitochondrial damage, inhibit the secretion of inflammatory factors and improve the function of renal tubular epithelial cells through regulating NF-κB signaling pathway.

Randomized controlled trials (RCT) have long been considered the gold standard for assessing clinical efficacy. However, RCT are inappropriate for some diseases due to related ethical issues and costs, such as rare diseases that are seriously life-threatening but without adequate treatment. Using real world data (RWD) as external control for RCT could make recruitment less complicated and reduce time and cost. This paper introduces common application scenarios, data sources, study design, basic principles, and statistical methods of RWD as an external control based on the latest guidelines related to RWD and combined with our team's previous research experience. This study could provide references for scholars and sponsors who want to conduct RWD research.

Objective To explore the application effects of comprehensive geriatrics assessment ( CGA) in diabetic patients. Methods A total of 110 elderly diabetic patients were selected as objects and were averagely divided into experimental group ( routine clinical nursing education and CGA nursing ) and control group ( routine clinical nursing education ) by random number table. Six months after the establishment of group, we used self-depression scale ( SDS ) , self-rated health measurement scale ( SRHMS ) to assess the score, and investigated fasting blood-glucose (FBG), 2 h postprandial blood sugar (2h PPBS), new incidence of complications and satisfaction of relatives. Results After nursing intervention 30, 60 and 90 d, the score of SDS in the experimental group was better than that of the control group (P<0. 05). The total SRHMS value of deviationwas(29.90±38.11),whichwashigherthan(3.25±10.58) inthecontrolgroup(t =7.007, P<0. 01). Conclusions CGA plays an apparent promotion for self-efficacy of elderly diabetic patients, and can effectively decrease patients′ blood glucose indicator, control a reasonable weight and blood pressure, increase relatives′satisfaction and be benefits patients′physical and mental health.