Objective To study relationship between the level of serum ICAM,ALCAM,VCAM and PECAM-1 in elderly patients with type 2 diabetes mellitus combined with acute cerebral infarction and its meaning.Methods From July 2014 to December 2015,60 patients with type 2 diabetes mellitus complicated with acute cerebral infarction were chosen as the research object.They were divided into three groups according to severity of atherosclerosis:no plaque in 11 cases,22 cases of stable plaques and unstable plaque 27 cases.40 cases with acute cerebral infarction patients and 40 cases of patients with type 2 diabetes were selected as a disease control;and other 40 healthy subjects were selected as healthy controls.ELISA were used to detect ICAM,ALCAM,VCAM and PECAM 1 level.Neural function evaluation was made by the U.S national institutes of health stroke scale (NIHSS).Results The ICAM,ALCAM,VCAM,PECAM 1 level in diabetic cerebral infarction group were higher than the other three groups,the difference was statistically significant (P＜0.05),while those of cerebral infarction group and diabetes group were higher than the control group,the difference was statistically significant (P＜0.05),and those of cerebral infarction group and diabetes group has no statistical significance (P＞0.05).NIHSS score of diabetic cerebral infarction patients was (7.39 ± 1.72),which was higher than that of patients with cerebral infarction group (5.33 ± 1.49),the difference was statistically significant (t=4.376,P =0.019,P＜0.05).The serum ICAM,ALCAM,VCAM,PECAM 1 level of diabetic cerebral infarction patients was a positively correlated with NIHSS score (r=0.559,P=0.007;r=0.619,P=0.000;r=0.421,P=0.018;r=0.451,P=0.007).With diabetic cerebral infarction is aggravating,the severity of carotid plaques in patients with serum ICAM,ALCAM,VCAM,PECAM-1 level is on the rise (P＜0.05).Conclusion ICAM,ALCAM,VCAM,PECAM-1 levels in peripheral blood serum of patients with type 2 diabetes mellitus complicated with acute cerebral infarction have an abnormal increase,and ICAM,ALCAM,VCAM,PECAM-1 level is closely related to the neurologic deficits and the severity of carotid artery plaque of type 2 diabetes mellitus complicated with acute cerebral infarction patients.

Objective To explore and analyze the effect of ambroxol hydrochloride combined with budesonide in treatment of children bronchial pneumonia on C-reactive protein level and WBC levels.Methods 100 cases of patients with bronchial pneumonia treated in our hospital from May 2015 to December 2016, were randomly divided into observation group and control group, 50 cases in each group.The control group were given routine treatment, while the observation group were given ambroxol hydrochloride combined with budesonide treatment on the basis of the control group,the course of treatment was 14 days.C-reactive protein and WBC levels were measured before treatment and 14 days after treatment, the Resultswere observed and statistical.ResultsThere were significant differences in the content of CRP between groups and multiple time points(F=11.50, P=0.003;F=4.78, P=0.03;F=5.45, P=0.02).The content of CRP 14 days after treatment in the observation group were significantly lower than the control group(t=14.12,P<0.05), the difference was statistically significant.There were significant differences in the WBC counts between groups and multiple time points(F=11.52, P=0.003;F=4.73, P=0.03;F=5.44, P=0.02),the difference was statistically significant.The count of WBC 14 days after treatment in the observation group after treatment were significantly lower than the control group(t=8.12, P<0.05), the difference was statistically significant.The number of cured, effective and ineffective cases in the control group was 20, 13 and 15, respectively.The observation group was 30, 15, and 5 cases, respectively.By rank sum test, the difference of the effective rate between the 2 groups was statistically significant(u=2.33, P<0.05).And the total effective rate in observation group was 90.0%, which was significantly higher than the control group(70.0%)(x2=6.25, P<0.05), the difference was statistically significant.Conclusion Ambroxol hydrochloride combined with budesonide in the treatment of bronchial pneumonia in children is effective and can effectively reduce the CRP and WBC count, so it is worth popularizing widely in clinic.

Objective:To study the correlation between chronic urticaria and Helicobacter pylori infection,and to explore the significance of HP detection in the diagnosis and treatment of chronic urticaria. Methods: Totally 420 cases of chronic urticaria,who were treated in outpatient department from April 2014 to July 2015,and 450 cases of healthy physical people were selected randomly as healthy control group in the same period,then the serum HP unease antibody was detected by colloidal gold method,the positive rate of two groups patients with HP was analysed. Meanwhile 162 chronic urticaria patients with positive HP were divided into experimental group with 88 cases and control group with 74 cases. The patients in the control group were treated with conventional treatment,while the experimental group were treated with triple therapy based on the treatment of control group,and the clinical efficacy of different ther-apeutic methods was analysed in the chronic urticaria patients with positive HP. Results: The positive rate of HP in chronic urticaria group was 38. 6%, and the positive rate of HP in healthy control group was 14. 4%, the difference was statistically significant ( P<0. 05). The effective rate of clinical efficacy in the experimental group was significantly higher than the control group(P<0. 05). Con-clusion:There is close correlation between chronic urticaria and HP infection,HP detection has important clinical significance for the diagnosis and treatment of chronic urticaria.

This article was aimed to study the analgesic and antipyretic effect of Re-Du-Ning (RDN) Injection. Heating model was building on SD rats after subcutaneous injection of yeast. And then, RDN Injection was intravenous injected at the dose of 10.16 g·kg-1, 5.08 g·kg-1, and 2.54 g·kg-1, respectively. Observation was made on changes of rats' temperature. RDN Injection was intravenous injected into ICR mice at the dose of 20.30 g·kg-1, 10.15 g·kg-1, 5.08 g·kg-1 for 7 consecutive days. The methods of mice twist, hot plate and jaw pain were used in the testing of analgesic action of RDN Injection. The results showed that RDN Injection at high and middle dose can significantly reduce the temperature of heating rats models (P < 0.05 or P < 0.01). It can also significantly reduce the twisting times of mice by acetic acid (P< 0.05 or P< 0.01). It can also significantly increase the pain threshold of mice by hot plate and jaw pain (P< 0.05 or P< 0.01). It was concluded that RDN Injection had a good analgesic and antipyretic effect in mice.

This study was aimed to discuss the antibacterial and immunomodulation effect ofRe-Du-Ning (RDN) injection. Mice were randomly divided into the normal group, model group,Shuang-Huang-Lian (SHL) group (7.8 mL·kg-1), RDN high dose group (20.30 g·kg-1), middle dose group (10.15 g·kg-1), and low dose group (5.08 g·kg-1), in order to observe the death protective effect of mice with bacterial infection on antibacterial experimentin vivo. Mice were randomly divided into the normal group, model group,Xiang-Gu Duo-Tang (XGDT) group (0.19 mg·kg-1), RDN high dose group (20.30 g·kg-1), middle dose group (10.15 g·kg-1), and low dose group (5.08 g·kg-1). The 2, 4-dinitrochlorobenzene was used to induce delayed hypersensitivity. Immunomodulation was observed by the content of serum hemolysin and the carbon particle clearance index. The results showed that the RDN high dose group and middle dose group had antibacterial effect, which reduced the mortality of mice. The RDN high dose, middle dose and low dose group can enhance the phagocytosis of macrophage in immunosuppressive mice, increase the formation of hemolysin, and strengthen delayed hypersensitivity reaction among immunocompromised mice. It was concluded that RDN injection had antibacterial effect. Its immunomodulation effect was through the enhancing of non-specific immunity, humoral immunity and cellular immunity of mice.

OBJECTIVE Toexplorethefeasibilityofpreparingamigrainemodelusingratdura materofsuperiorsagittalsinusinfollowingelectricalstimulation.METHODS MaleSDratswereexposed to 1 h electrical stimulation after brain electrode implantation,at 3 V,6 Hz,and a pulse of 0.25 ms.At 0, 1 5,30 min,and 1 h,orbital blood and brain tissue were taken.The content of calcitonin gene-related peptide (CGRP),substance P and prostaglandin E2 (PGE2 )in the plas ma and brain tissue was detec-tedbyradioimmunoassay.RESULTS ThebraintissuecontentofCGRP,substancePandPGE2in the sham group and normal group was not significantly different.CGRP,substance P and PGE2 in brain tissue of model group were 3.41 ±0.93,1 .80 ±0.64,3.41 ±0.93 and (1 .80 ±0.64)ng·g -1 respec-tively,significantly different from sham group(P<0.05).At different time points,the content of CGRP, substance P and PGE2 in plasma orbital venous plexus showed no significant difference between sham groupandmodelgroup.CONCLUSION ThereleaseofCGRP,PGE2andsubstancePinbraintissue is significantly different fro m that of plas ma after electrical sti mulation.The results suggest that a model of migraine can be constructed by electrical stimulation.

Objective To investigate the potential prognostic factors in patients with hepatocellular carcinoma (HCC) complicate by portal vein tumor thrombosis (PVTT) who are treated with transcatheter arterial chemoembolization (TACE). Methods The complete clinical data of a total of 46 patients with HCC complicate by PVTT, who were treated with TACE during the period from January 2010 to March 2016, were retrospectively analyzed. Clinical material database was established. Kaplan-Meier test was adopted to analyze the survival rate and the COX risk ratio model was used to screen out the independent prognostic factors. Life table method was employed to calculate the survival time. Results The 6-, 12-, 18- and 24-month survival rates were 51. 2％, 28. 9％, 23. 4％ and 10. 2％, respectively. The median survival time was 6. 7 months. According to mRECIST standard, complete remission (CR) was obtained in one patient (2. 1％), partial remission (PR) in 11 patients (23. 9％), stable disease (SD) in 16 patients (34. 8％) and progress disease (PD) in 18 patients (39. 2％). Multivariate analysis indicated that local tumor response, ascites, cholinesterase, and arteriovenous fistula were the independent factors affecting the prognosis. Conclusion The independent prognostic factors that affect the survival time of HCC patient include local tumor response, ascites, cholinesterase and arteriovenous fistula. (J Intervent Radiol, 2018, 27: 266-271)

ObjectiveTo analyze the epidemiological characteristics of hepatitis B virus （HBV） infection among blood donors in Hainan Province. MethodsHBV screening was conducted on 6 851 samples from voluntary blood donors in Hainan Province from January 2020 to January 2023. The samples were grouped according to donation frequency， method， age， gender， occupation， and education level， and risk factors related to HBV infection and genotypes were analyzed. ResultsThe HBV infection rate among first-time blood donors （3.59%） was higher than that among repeat donors （0.16%）. Among first-time blood donors， the HBV infection rate in males （2.22%） was higher than in females （1.66%）， and the infection rate increased with age （all P<0.05）. Blood donors who were teachers （1.18%）， militaries （1.01%）， civil servants （1.27%）， and medical staffs （0.85%） had lower HBV infection rate， while workers （6.05%）， farmers （6.15%）， and freelancers （7.61%）， and service industry workers （8.77%） had higher HBV infection rate. The infection rate decreased with higher education levels （P<0.05）. Multivariate logistic regression analysis showed that multiple blood donations （OR=0.623， 95%CI： 0.337‒0.945）， history of intravenous transfusions （OR=1.694，95%CI： 1.517‒1.946）， and family history of HBV infection （OR=1.768， 95%CI： 1.265‒1.934） were independent risk factors for HBV infection （all P<0.05）. Significant differences were observed among different HBV genotypes in terms of HBeAg positivity rate， HBeAb positivity rate， incidence of post hepatitis B cirrhosis， as well as levels of ALT， AST， and HBV-DNA （all P<0.05）. The results of multivariate logistic regression analysis showed that age， HBeAg， HBV-DNA， and clinical type were risk factors for HBV genotype distribution （P<0.05）. ConclusionHainan Province is a low-prevalence area for HBV infection， but the latent risk for HBV infection still exists. Therefore， it is important to thoroughly understand the basic information and HBV infection genotypes of blood donors， so as to select healthy blood donors and reduce the discovery and transmission of HBV-positive cases among blood donors.

Objective To investigate the changes of soluble lectin-like oxidized low-density lipoprotein receptor-1 (sLOX-1) and omentin-1 in patients with H-type hypertension complicated with acute ischemic stroke, and to analyze the correlation of sLOX-1 and omentin-1 levels with the severity and prognosis of the disease.Methods Totally 136 patients with H-type hypertension complicated with acute ischemic stroke from February2017 to May 2018 were selected as observation group, and 136 non-acute ischemic stroke patients with H-type hypertension in the same period as the control group. The patients of observation group were divided into mild, moderate and severe sub-groups according to NIHSS score, and they were also divided into good prognosis group and poor prognosis group based on modified RANKIN scale (mRS) score. The serum sLOX-1 and omentin-1 levels were detected, and the correlation of sLOX-1 and omentin-1 levels with severity and prognosis of disease was analyzed. Results The serum sLOX-1 level of the observation group was higher, but the serum omentin-1 level lower than that of control group (P < 0.05). With the severity of the disease, the serum sLOX-1 level increased, but the serum omentin-1 level decreased (P < 0.05). The serum sLOX-1 level of good prognosis group was significantly lower, whereas the serum omentin-1 level significantly higher than that of poor prognosis group (P < 0.05). sLOX-1 was positively correlated with NIHSS score and mRS score, while omentin-1 was negatively correlated with NIHSS score and mRS score (P < 0.05). Conclusions The levels of serum sLOX-1 and omentin-1 are closely related to the severity and prognosis of patients with H-type hypertension complicated with acute ischemic stroke, which could be used as markers for evaluating the severity and prognosis of the patients.

Objective:To investigate the relationship between single nucleotide polymorphism (SNPs) of Zeste homolog enhancer 2 (EZH2) gene and the risk of breast cancer.Methods:Recruiting 1 039 breast cancer patients and 1 040 controls at 22 referral hospitals nationwide in China, the genotype distribution of 3 SNPs loci of EZH2 genes was observed to detect the correlation between different genotypes and the risk of breast cancer genotypes EZH2 expression in breast cancer tissues and its correlation with patient prognosis were analyzed using breast cancer data from the database.Results:EZH2 rs6464926 CC genotype was compared with TT genotype (TT vs. CC: OR=1.362, 95% CI: 1.063-1.746, P=0.015) and dominant model (TC+TT vs .CC: OR=1.22, 95% CI: 1.004-1.483, P=0.045) .In women with BMI ≥24 kg/m 2, the TC genotype ( P=0.050), TT genotype ( P=0.025) and dominant model (TC+TT, P=0.021) of rs6464926 locus were significantly different from CC genotype in cancer risk. rs6464926 was correlated with EZH2 gene expression ( P=6.89E-47). EZH2 gene is highly expressed in breast cancer tissues, and patients with high expression were associated with shorter OS ( HR=1.27, P=0.013), DMFS ( HR=1.37, P<0.01), and RFS ( HR=1.44, P<0.01). Conclusions:The polymorphism rs6464926 of EZH2 gene is associated with breast cancer susceptibility in Chinese women. rs6464926 might regulate breast cancer risk and prognosis by changing EZH2 expression.