1.Clinical study of modified Shenghua Decoction combined with ethinylestradiol and cyproterone acetate tablets for the polycystic ovary syndrome patients with blood-insufficiency congealing-cold and blood-stasis syndrome
International Journal of Traditional Chinese Medicine 2021;43(5):457-461
Objective:To discuss the clinical efficacy of Shenghua Decoction combined with conventional western medicine therapy in treatment of polycystic ovarian syndrome with blood-insufficiency congealing-cold and blood-stasis syndrome. Methods:A total of 140 polycystic ovarian syndrome patients with blood-insufficiency congealing-cold and blood-stasis syndrome were selected from January 2018 to December 2019 in our hospitals, and were divided into observation group and control group according to the random indicator method, 70 cases in each group. The control group received ethinylestradiol and cyproterone acetate tablets treatment, and Shenghua Decoction was added based on the control group’s treatment in the observation group. Changes in the TCM syndrome score, modified Ferriman-Gallway (mF-G) of hirsutism, Rosenfield of acne, Testosterone (T), Follicle-Stimulating Hormone (FSH) and Luteinizing Hormone (LH) levels detected by ELISA of two groups were observed. The adverse events were recorded and the clinical efficacy was evaluated. Results:The effective rate of the observation group was 88.6% (62/70), which was significantly higher than control group of 60.0% (42/70) ( χ2=14.957, P<0.001). After treatment, mF-G, Rosenfield and TCM Syndrome Scores in the observation group were significantly lower than those in the control group ( t=6.524, 3.111, 2.196, P<0.01 or P<0.05). After treatment, the levels of FSH [(5.16 ± 1.76) IU/L vs. (7.19 ± 1.06) IU/L, t=2.530], LH [(6.12 ± 1.88) IU/L vs. (8.03 ± 1.78) IU/L, t=2.403], T [(1.34 ± 0.32) μg/L vs. (1.80 ± 0.36) μg/L, t=7.080] in the observation group were significantly lower than those in the control group ( P<0.05 or P<0.01) Conclusion:The Shenghua Decoction showed beneficial effect for the patients with polycystic ovarian syndrome with blood-insufficiency congealing-cold and blood-stasis syndrome.
2.Development of a multiple PCR-based reverse line blot hybridization assay(mPCR-RLB)to detect seven sexually transmitted disease pathogens
Hui WANG ; Fanrong KONG ; Xiaoyong ZHOU ; Xianyu ZENG ; Weizhen WANG ; Yiqun DUAN ; Zhiliang ZENG ; Chunmei CHEN ; L.gilbert GWENDOLYN
Chinese Journal of Dermatology 2008;41(12):810-813
Objective To develop a multiple PCR-based reverse line blot hydrization assay (mPCR-RLB)to simutaneously detect several STD pathogens:Neisserria gonorrhoeae,Chlamydia trachomatis,Ureaplasma urealyticum,U.parvum,Mycoplasma genitalium,M,hominis and Trichomonas vaginalis.Methods Seven pairs of biotin-labelled primer were designed and synthesized to target the 16S rRNA-23S rRNA intergenic spacer regions of Neisserria gonorrhoeae,Chlamydia trachomatis,Mycoplasma,and repetitive DNA sequence of Trichomonas to identify and subtype thesc pathogens.DNA was extracted from the referrence strains of seven pathogens and used as templates.mPCR was performed to simutaneously amplify the target regions of these pathogens.Then,the biotin-labelled amplicons were hybridized with membrane-bound specific oligonucleotide probes followed by the detection of bound amplicons with chemiluminescence assay.Serially diluted plasmids containing the target genes of pathogens were amplified with this method to detect its sensitivity.Two-hundred and eleven specimens,including 104 male urethral swabs and 107 female cervical swabs,were collected from the STD clinic of Wuhan First Hospital;mPCR-RLB and single-primer PCR were performed.For specimens with inconsistent results,nested PCR was performed to confirm the results.Results The assay sensitively and specifically identified referrence strains of the tested pathogens.The detection limit of mPCR-RLB was 100 copies for all the pathogens.Of the 211 clinical specimens,2.8%(6/21)were negative for single-primer PCR,but positive for mPCR-RLB,and nested-PCR results were consistent with those of mPCR-RLB.Conclusion mPCR-RLB is a sensitive,specific and rapid method for the detection of STD pathogens from clinical specimens.
3.Correlation of vascular endothelial cell dysfunction to vascular restenosis following coronary artery stent implantation
Jinchuan LAI ; Biao CHENG ; Jie LI ; Hong KONG ; Yan SHU ; Jianhong TAO ; Rongjian JIANG ; Chunmei LI ; Yan DENG
Chinese Journal of Tissue Engineering Research 2008;12(22):4357-4360
BACKGROUND: Dysfunction of endothelial cells is independently associated with coronary atherosclerotic heart disease. Correlation of dysfunction of endothelial cells to restenosis after stent implantation is not yet clearly determined.OBJECTIVE: To evaluate the correlation of vascular endothelial dysfunction to restenosis after stent implantation. DESIGN, TIME AND SETTING: A case control study was performed at the Department of Cardiology and Department of Heart Ultrasound, Sichuan People's Hospital from March 2005 to January 2007.PARTICIPANTS: After review, coronary angiography showed that 11 patients who occurred restenosis at the lesion region after stent implantation were included in a restenosis group, and an additional 15 patients who did not develop in-stent restenosis were included in a control group. Patients in the following conditions were excluded: over 70 years old, histories of long-term smoking, diabetes mellitus, multivessel disease, long coronary lesion and chronic total occlusion, heart failure (Killip's class Ⅲ or above), severe hepatic and/or renal insufficiency.METHODS: High-resolution ultrasound was used to assess the percentage of flow-mediated dilatation of brachial artery. Differences in endothelial function were compared between both groups.MAIN OUTCOME MEASURES: Ultrasound parameter of the brachial artery in both groups; Partial correlation analysis on control variable including sex, age, blood lipid level, diseased region and stent type.RESULTS: No significant difference was found in basic diameter of brachial artery in both groups. During reactive hyperemia, inner diameter and its absolute variation of brachial artery were smaller in the restenosis group than in the control group (P<0.01). The percentage of brachial artery flow-mediated dilatation was lower in the restenosis group compared with the control group (P=0.013). The partial correlation coefficient between the percentage of flow-mediated dilatation of brachial artery and in-stent restenosis was 0.47 (P=0.04).CONCLUSION: The endothelial dysfunction significantly decreases in patients with restenosis compared with controls following stent implantation. There is a correlation between endothelial dysfunction and restenosis.
4.Status quo of the selection of intravenous infusion devices in hospitalized children
Qingqing ZHANG ; Ying GU ; Yingwen WANG ; Chunmei LU ; Meijing KONG
Chinese Journal of Modern Nursing 2024;30(14):1923-1927
Objective:To investigate the status quo of intravenous (IV) infusion device selection among hospitalized children and provide direction for improving practices related to the selection of infusion devices.Methods:A total of 1 306 hospitalized children undergoing IV infusion treatment in 11 clinical departments of Children's Hospital of Fudan University in June 2021 were selected by convenience sampling. A self-developed data collection form for the selection of IV infusion devices in hospitalized children and criteria for the appropriateness of IV infusion device selection were used to survey and evaluate the appropriateness of IV infusion device selection among these children.Results:IV infusion devices were found to have been appropriately selected in 1 137 of the 1 306 children, while these devices were inappropriately selected in 169 children. The inappropriate selection was primarily due to the improper choice of peripheral intravenous catheters (PIVC), with 155 cases involving the administration of non-peripheral compatible medications through PIVC. No significant statistical difference was found in the appropriateness of IV infusion device selection between the infant group and the child and adolescent group ( P>0.05). Significant differences were observed in the appropriateness of IV infusion device selection based on different physicochemical properties of medications and the duration of therapy ( P<0.01) . Conclusions:The standardization of IV infusion device selection among hospitalized children needs improvement. It is urgent to apply evidence from the Clinical Practice Evidence- Based Guidelines for Pediatric Intravenous Therapy regarding recommendations for IV infusion device selection, to initiate evidence application projects, and to standardize the selection of IV infusion devices.
5.Clinical study of Tongren Niuhuang Qingxin Pills combined with Telmisartan in the treatment of hypertensive vertigo syndrome of phlegm-heat disturbance
Chunmei YUE ; Yanling XIAO ; Xiaohua LONG ; Fanfei KONG ; Xiaotong XU ; Yanjiao FENG ; Jingjing ZHAO ; Quan LIU ; Chunjiao DONG ; Ming TANG ; Yang YANG
International Journal of Traditional Chinese Medicine 2024;46(5):588-593
Objective:To observe the clinical efficacy and safety of Tongren Niuhuang Qingxin Pills combined with telmisartan tablets in the treatment of hypertensive vertigo syndrome of phlegm-heat disturbance.Methods:Randomized controlled trial was conducted. Totally 80 patients with hypertension vertigo and phlegm-heat disturbance syndrome were selected from March 2021 to August 2022 at Beijing Tongrentang Hospital of Traditional Chinese Medicine as the observation objects. They were randomly divided into two groups using a random number table method, with 40 cases in each group. The control group received oral telmisartan tablets, while the experimental group received Tongren Niuhuang Qingxin Pills in addition to the control group. Both groups were treated for 28 days and followed up for 1 month. The patients' room blood pressure before and after treatment was measured, and TCM syndrome scores were evaluated. The dizziness assessment rating scale (DARS) was used to evaluate the severity of dizziness, adverse reactions during treatment were recorded, drug safety was observed, and clinical efficacy was evaluated.Results:The total effective rate of the experimental group was 85.0% (34/40), and that of the control group was 7.5% (3/40), with statistical significance between the two groups ( χ2=48.32, P<0.001). Compared with before treatment, the experimental group had SBP [(136.63 ± 6.01) mmHg vs. (159.30 ± 9.01) mmHg, t=-21.00] and DBP [(84.48 ± 4.36) mmHg vs. (95.30 ± 3.75) mmHg, t=-13.80] after treatment; after treatment, SBP [(137.34 ± 6.39) mmHg vs. (158.00 ± 10.06) mmHg, t=-5.28] and DBP [(86.08 ± 4.43) mmHg vs. (95.18 ± 6.61) mmHg, t=-8.09] decreased in the control group ( P<0.01), but there was no statistical significance between the two groups after treatment ( P>0.05). After treatment, the TCM syndrome scores in the experimental group (8.68 ± 3.39 vs. 15.12 ± 3.03, Z=-6.61) were lower than those in the control group ( P<0.001), and DARS score [(8.53 ± 3.93) vs. (12.20 ± 3.95), Z=-3.63] was lower than that in the control group ( P<0.001). After treatment, the therapeutic effect index of TCM syndromes in the experimental group improved compared to before treatment in the same group. The therapeutic effect index of each symptom, from high to low, was as follows: rotation of oneself or visual objects>numbness of limbs>dry stool>dizziness and dizziness>liking cold drinks>bitter and dry mouth>red urine>red tongue, yellow coating, and greasy tongue>vomiting sticky and turbid phlegm>tinnitus>smooth pulse. There were no significant adverse reactions during the treatment of the two groups. Conclusion:Tongren Niuhuang Qingxin Pills combined with telmisartan can reduce the blood pressure of patients with hypertensive vertigo syndrome of phlegm-heat disturbance, improve the vertigo symptoms and TCM syndromes of patients, and the efficacy evaluation is superior to that of telmisartan alone.
6.Analysis of the salt-stress responsive element of the promoter of peanut small GTP binding protein gene AhRabG3f.
Guoning DU ; Jie XIANG ; Shunyu LIN ; Xiangyuan KONG ; Xiuling WU ; Xuedong GUAN ; Hong ZHU ; Jingshan WANG ; Lixian QIAO ; Jiongming SUI ; Chunmei ZHAO
Chinese Journal of Biotechnology 2022;38(8):2989-2998
To study the molecular mechanism of salt stress response of peanut small GTP binding protein gene AhRabG3f, a 1 914 bp promoter fragment upstream of the start codon of AhRabG3f gene (3f-P) from peanut was cloned. Subsequently, five truncated fragments (3f-P1-3f-P5) with lengths of 1 729, 1 379, 666, 510 and 179 bp were obtained through deletion at the 5' end, respectively. Plant expression vectors where these six promoter fragments were fused with the gus gene were constructed and transformed into tobacco by Agrobacterium-mediated method, respectively. GUS expression in transgenic tobacco and activity analysis were conducted. The gus gene expression can be detected in the transgenic tobacco harboring each promoter segment, among which the driving activity of the full-length promoter 3f-P was the weakest, while the driving activity of the promoter segment 3f-P3 was the strongest. Upon exposure of the transgenic tobacco to salt stress, the GUS activity driven by 3f-P, 3f-P1, 3f-P2 and 3f-P3 was 3.3, 1.2, 1.9 and 1.2 times compared to that of the transgenic plants without salt treatment. This suggests that the AhRabG3f promoter was salt-inducible and there might be positive regulatory elements between 3f-P and 3f-P3 in response to salt stress. The results of GUS activity driven by promoter fragments after salt treatment showed that elements included MYB and GT1 between 1 930 bp and 1 745 bp. Moreover, a TC-rich repeat between 682 bp and 526 bp might be positive cis-elements responsible for salt stress, and an MYC element between 1 395 bp and 682 bp might be a negative cis-element responsible for salt stress. This study may facilitate using the induced promoter to regulate the salt resistance of peanut.
Arachis/genetics*
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Fabaceae/genetics*
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GTP-Binding Proteins/metabolism*
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Gene Expression Regulation, Plant
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Glucuronidase/metabolism*
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Plant Proteins/metabolism*
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Plants, Genetically Modified/genetics*
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Salt Stress
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Stress, Physiological/genetics*
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Tobacco/genetics*