Objective: To study laparoscopic surgery for ectopic pregnancy complicated by hemorrhagic shock clinical efficacy and nursing research. Methods: Retrospective analysis of 60 cases of ectopic pregnancy complicated by hemorrhagic shock patients, divided into:the experimental group and control group of 30 cases, control group using traditional open surgery, laparoscopic surgery treatment groupclosely observe the clinical efficacy of the two groups. Results: Laparoscopic treatment of ectopic pregnancy in patients with hemorrhagic shock complicating operative time, blood loss than the control group; laparoscopic treatment of ectopic pregnancy in patients with hemorrhagic shock complicated exhaust time, postoperative morbidity and hospital stay was significantly lower than control group. Conclusion: Ectopic pregnancy laparoscopic surgery can improve concurrency cure rate of patients with hemorrhagic shock, better retention in patients with reproductive function, improve the patient's quality of life.

Epigenetics change has been considered to be the most promising new strategy for disease control and prevention. TCM regulates gene expression through epigenetics, participating in pathological and physiological process including cell apoptosis, proliferation, differentiation, cell cycle regulation, immunity, inflammation, and metabolism. This article reviewed the application of DNA methylation, histone modification and the miRNA regulation in TCM research.

OBJECTIVE:To put forward policy suggestions on the selection and revision of National Essential Drug List (NEDL) in China. METHODS: The selection principles, criteria and procedures of WHO Model List of Essential Medicines and Australia’s Pharmaceutical Benefits Scheme Directory which was commonly regarded as Essential Drug List of Australia were analyzed to provide reference for the selection and revision of NEDL in China. RESULTS & CONCLUSION: The selection and revision of NEDL which were strongly supported by related policies demand active participation of all social sectors, open and transparent working procedures, scientific and operational methods for selection and revision, authoritative monitoring and aftereffect evaluation system.

Magnetic susceptibility is an intrinsic physical quantity which describes the relationship between material magnetization and applied external magnetic field. Quantitative susceptibility mapping (QSM) is an MRI technology which can quantify the buck magnetic susceptibility of tissue in vivo. It is particularly effective at elucidating anatomy with paramagnetic or diamagnetic components. QSM technology is a method for solving the ill-pose problem of unconventional de-convolution of the measured tissue magnetic field with the unit magnetic dipole field to obtain the susceptibility source map. Many multi orientation scan based QSM and clinically acceptable single orientation QSM methods have been proposed to solve this ill-posed problem. In this paper, the QSM concept is introduced and the various QSM methods are systematically categorized and discussed. The aim of this paper is to summarize the current research progress of QSM, popularize the knowledge of QSM and promote the improvements and the rational application of QSM in clinical field.
Humans ; Magnetic Fields ; Magnetic Resonance Imaging ; trends ; Magnetics

Objective To retrospectively evaluate the utility of apparent diffusion coefficient (ADC) values in predicting aggressiveness of prostate cancer.Comparison was made with transrectal ultrasound-guided biopsy Gleason scores (GS) and prostatectomy GS.Methods Diffusion weighted images of 51 patients with biopsy-proven prostate cancer were obtained using 1.5 T MR with a pelvic phased-array coil.Regions of interest (ROIs) were drawn on areas of the suspicious lesion and the ADC values were calculated.The correlations between the ADC values and prostatectomy GS were assessed with Pearson correlation.The relationship between biopsy GS and prostatectomy GS were also evaluated.Meanwhile,receiver operating characteristic (ROC) curves were used to determine the ability of ADC values and biopsy GS in differentiating low-grade prostate cancer from intermediate/high grade prostate cancer.Results The accuracy of transrectal ultrasound-guided biopsy in predicting prostatectomy GS was 41.2％(21/51).Compared with prostatectomy GS,up to 11.8％ of the patients (n =6) was overestimated by biopsy,while 47.0％ (n =24) were underestimated.These 51 patients had a mean ADC value of (0.974 ±0.194) × 10-3 mm2/s.The mean ADC value of intermediate/high-grade tumors (n =35) was (0.907 ±0.160) ×10-3 mm2/s while that of low-grade tumors was (1.121 ±0.185) × 10-3 mm2/s (n =16).A significant negative correlation was found between mean ADC values of suspicious lesions and their prostatectomy GS (r =-0.761,P ＜ 0.01).No significant correlation was found between biopsy GS and prostatectomy GS (r =0.187,P =0.189).The area under the ROC curves of ADC and biopsy GS was 0.827 and 0.689,respectively.Conclusion The ADC values of cancerous areas in prostate perform better than biopsy GS in predicting aggressiveness of prostate cancer.

Objective To evaluate the effects of combination of dexmedetomidine and mild hypothermia on global cerebral ischemia-reperfusion (I/R) injury in neonatal rats.Methods Ninety-six neonatal Sprague-Dawley rats,aged 6-7 days,weighing 18-22 g,were randomly divided into 4 groups (n=24 each) using a random number table:I/R group,mild hypothermia group (group H),dexmedetomidine group (group D) and combination of dexmedetomidine and mild hypothermia group (group DH).Global cerebral ischemia was induced in rats anaesthetized with chloral hydrate by bilateral common carotid artery clamping (for 15 min) combined with hypotension followed by reperfusion.Dexmedetomidine 75 pg/kg was given intraperitoneally at 30 min before ischemia in D and DH groups,while the equal volume of normal saline was given in I/R and H groups.The temperature in the temporal muscle was maintained at 36.7-37.2℃ in I/R and D groups,and at 34.8-35.3℃ in H and DH groups.At 12,24 and 72 h of reperfusion,8 rats were randomly chosen in each group,and neurological deficit score (NDS) was determined.The animals were then sacrificed,and their brains were removed for determination of myeloperoxidase (MPO) activity (by spectrophotometry) and contents of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) in brain tissues (using ELISA).Results Compared with I/R group,the NDS,MPO activity and contents of TNF-α and IL-6 were significantly decreased in the other three groups.The NDS,MPO activity and contents of TNF-α and IL-6 were significantly lower in DH group than in H or D group.Conclusion Dexmedetomidine can optimize cerebral protection providedby mild hypothermia against global cerebral I/R injury through inhibiting inflammatory responses in brain tissues of neonatal rats.

Objectives To investigate the feasibility of the quantitative analysis parameters of dynamic contrast-enhanced MRI (DCE-MRI) in the diagnosis of prostate cancer and to correlate Ktrans, Ve and kep with histological grade (Gleason score) and serum prostate specific antigen (PSA). Methods MR examinations were performed in 38 patients confirmed by biopsy. ROI were drawn on areas of cancerous foci,noncancerous foci in the peripheral zone and central gland to measure the values of Krans, Ve and kep. The values of the three parameters in different tissue were compared with ANOVA. The correlations between the pharmacokinetic parameters and Gleason score, PSA were assessed with Pearson correlation. Results The Ktrans, Ve, kep values of cancerous foci were (0. 35 ± 0. 26 )/min, ( 0. 185 ± 0. 080 ), ( 1.42 ± 0. 53 )/min,respectively, while (0. 07 ± 0. 05 )/min, ( 0. 040 ± 0. 024 ), (0. 50 ± 0. 18 )/min for noncancerous foci in the peripheral zone and (0. 19 ±0. 06)/min, (0. 161 ± 0. 062), (0. 94 ±0. 25) /min for noncancerous foci in the central gland, respectively. The differences between the three parameters of cancerous foci,noncancerous foci in the peripheral zone and central gland were statistically significant ( F = 16. 267,44. 084, 36. 095, respectively; P ＜ 0. 01 ). No significant correlations were found between any parameter and either Gleason score or PSA (r =0. 279,0.069, 0. 109, -0. 175, -0.067,0. 137, respectively;P ＞0. 05). Conclusion Quantitative analysis parameters of DCE-MRI are feasible in diagnosing prostate cancer. They may be useful in differentiating prostate cancer from benign prostatic diseases.

ObjectiveTo explore the prevalence and clinical features of hyperuricemia and its influencing factors in elderly male people aged 90 years and above. MethodsOne hundred elderly male people aged 90 years and above who underwent routine health examination in our hospital in 2007 were selected in the study. Serum uric acid level was examined by uricase-peroxidase method, and all patients were divided into hyperuricemia group and control group according to the serum uric acid level. Clinical and biochemical indications were compared between the two groups, and logistic regression was used to analyze the influencing factors of hyperuricemia in elderly people. ResultsThe serum uric acid level was increased in 20% of the elderly people, and the prevalence of gouty arthritis was 1%. The levels of blood urea nitrogen and serum creatinine were higher in hyperuricemia group than in control group[(10. 98±4.29) mmol/L vs. (6. 87± 1.86) mmol/L, (125.2±25.9)μmol/L vs. (93. 4±19. 8)μmol/L;both P<0.05)3. The patients in hyperuricemia group had a higher prevalence of hypertension and hypertriglyceridemia, and a higher proportion of diuretic application than patients in control group(P<0. 05). Logistic regression analysis showed that serum uric acid level had the most remarkable correlation with serum creatinine(OR= 1. 969), followed by fasting blood glucose (OR= 1. 310)and blood urea nitrogen(OR = 1.161). There was negative correlation between serum uric acid level and plasma cholesterol level(OR = 0. 802). ConclusionsThe prevalence of hyperuricemia is high in elderly people aged 90 years and above, while the incidence of gouty arthritis is low. Renal function impairment, metabolic syndrome and thiazide diuretic are the major factors for hyperuricemia.

Objective To investigate the neuroprotective effect of lateral intracerebroventricular injection of Apelin-13 on the YAP expression and the cerebral ischemia/reperfusion (I/R) injury in Wistar rats.Methods Healthy adult male Wistar rats were randomly divided into the sham group, cerebral I/R group and Apelin-13 treatment group.The middle cerebral artery occlusion model was established with ischemia for 1 hour and reperfusion for 24 hours after restricting food and water intake for 12 hours.Apelin-13 was injected into rats' lateral ventricles of Apelin-13 treatment group after reperfusion.Neural function defects was assessed.The volume of infarction was evaluated by TTC staining.The expression levels of YAP were detected by western blot.Results Compared with the cerebral I/R group,the rats in the Apelin-13 treatment group had abetter neurologic score ((2.67±0.33) vs (1.67±0.33) , P＜0.05), the infarction volume was decreased ((30.60± 1.42) ％ vs (23.70± 2.20) ％,P＜0.05) , and YAP expression level was increased in each part of the cerebral tissue(P＜0.05).Conclusion Apelin-13 has a neuroprotective effect,which plays the therapeutic effect by regulating the expression of YAP on cerebral ischemia/ reperfusion (I/R) injury in Wistar rats.

Objective To identify novel markers such as THRA or TCL1 in Sj(o)gren's syndrome (SS) patients to discriminate them from hcalthy volunteers.Methods Experimental group (n=40) and healthy volunteer group (n=40) were recruited based on strict inclusion and exclusion criteria.Peripheral blood samples (9 ml) were collected and peripheral blood mononuclear cells were separated by Ficoll gradient centrifugation.RNA was extracted using Trizol reagent,and the expression of THRA,TCL1 in PBMCs was detected by real-time PCR.And the data were processed with Wilcoxon test and t test (P＜0.05).Results TCL1 and THRA expression level are higher in SS patients (2.5±4.7) than healthy volunteers (Z=-2.045,P＜0.05).The TCLI expression level (4.4±3.8) is higher in high lymphadenopathy activation index patients (2 to 3grade) than that in low lymphadenopathy activation index patients (grade 0 to 1 ) (t=-2.319,P＜0.05 ).Conclusion TCL1 expression level is higher in SS patients,and TCL1 expression level is related to the severity of lymphadenopathy,which provide a new platform of the study for the pathogenesis,disease severity evaluation,even dia-gnosis and treatment of SS.