BACKGROUND: The image edge of rabbit femur is identified using self-programmed Matlab program. But owing to the complexity of the model, can we set up a femoral structure model after the solid model of the femur created in UG three-dimensional (3D) software is put into ANSYS software? OBJECTIVE: To explore the method of constructing 3D biomechanical model of rabbit femur.DESIGN: Randomized controlled observation.SETTING: Biomechanics Laboratory of Beijing Institute of Technology.MATERIAL: One 1-year-old female New Zealand white rabbit weighing 2.6 kg. ACTIS 400/225 industry CT scanner (BIR,USA).METHODS: The experiment was performed at the Biomechanics Laboratory of Beijing Institute of Technology from July to transformed into *.bmp format. Photoshop image processing software was applied for some essential processing on the original image, such as contrast control, smoothing and noise attenuation to strengthen the differentiability and analyzability of the image. In addition, the magnetic noose tool was used to outline the contour line of inside and outside boundary, and obtain the inside and outside contour line of every layer of the femur. The rabbit femur was scanned by industry CT starting from the upper extreme of the femur, vertical to femoral ordinate axis line (slice space=I ram). The scanning slice space was adjusted to 2 mm from the 43rd layer below small trochanter to the 53ra layer of middle femur because the shape between these layers was relatively regular. Totally 87 slices of layer images were scanned 9.9 cm in length. The image was put into the computer and processed to get the boundary contour line; the contour line coordinate for modeling was obtained through imported from UG into ANSYS software. A solid model of the femur was produced automatically. Finally, the solid model with cavity was obtained using subtraction option of Boolean in ANSYS.MAIN OUTCOME MEASURES: Establishment of rabbit femoral structure model. connected by the multiple valid points was formed. The 2nd, 3rd, 4th until the 87th layers were read in turn, and the inside and automatically divide the mesh to produce finite element meshes and 42 221 nodes and 27 768 elements were obtained. CONCLUSION: The 3D model of rabbit femur actually simulated the anatomic appearance of the femur. This model lays a foundation for identifying the optimal parameter of vibration that can improve bone mineral density by finite element analysis.

Osteosarcoma and ewing sarcoma are the two most common types of primary malignant bone tumors. Receptor tyrosine kinases(RTKs),including varieties of cell growth factors and proto-oncogene pro-ducts,are the high-affinity cell surface receptors which play an important role in the normal physiological func-tion of cells. A large number of studies have demonstrated that the abnormal RTKs signaling pathways may pro-mote the tumorigenesis and development of osteosarcoma and ewing sarcoma by affecting tumor cell survival, proliferation,invasion and metastasis. Targeting treatment of RTKs is a promising therapeutic approach for osteosarcoma and ewing sarcoma.

Objective:To explore the medical undergraduate teaching method of ECG. Methods:random sampling in the practice of medical undergraduates in our hospital ,2013 medical undergraduates as the experimental group, 2012 medical undergraduates as control group, two groups of 40 (male, female 20), age 22~23 years old, the experimental group internship period adjustment of ECG in 2 week internship, ECG during the internship period, by the junior high the doctor, according to a set of prior to the development of the teaching plan, practice teaching, the control group had no ECG internship content; in the two groups before and after the end of internship, were an ECG, theory of knowledge, operation and map reading test. Results:In the experimental group in the ECG theoretical knowledge, operation method, normal electrocardiogram, abnormal electrocardiogram exam scores, were more than that of the control group, there was significant difference in two groups(x2=22.321, x2=37.883, x2=31.179, x2=10.66;P<0.001). Conclusion: According to undergraduate medical situation, summarize the clinical teaching process ideas, enhance the practice ability, is before the practice was significantly increased in the reading and analysis abilities of ECG, far more than the control group. The teaching plan is feasible and effective;it is worth further exploration and promotion.

Objective To explore the correlation between social support and subjective well-being of Zhuang elder people in rural area.Methods 320 Zhuang nationnality rural old man were randomly selected from the xiangxian town,the Guangxi Zhuang Autonomous Region.Left behind elderly among them (A group) was 160,non left behind elderly (B group) was 80,five elderly (C group) was 80.General access volume,Memorial University of Newfoundland scale of happiness (MUNSH) and social support rating scale (SSRS) were investigated.Results Memorial University of Newfoundland scale of happiness scores of A,B,C groups were (27.90± 10.61,35.11 ±9.92,31.67± 12.07),and the difference was statistically significant (F=11.45,P＜ 0.05).SSRS scores of A,B,C groups were (34.91±8.13,39.53±6.95,30.31±7.62),the difference was statistically significant (F=26.53,P＜0.05).A,B group's happiness score and objective support was positively correlated (r=0.20,0.25 ; P＜0.05),A,B,C group's happiness score is positively correlated with subjective support (r=0.17,0.36,0.31; P＜0.05),happiness score and utilization degree positive correlation in A,C groups (r=0.23,0.39; P＜0.05).Conclusion Zhuang rural elderly subjective well-being is associated with each dimension of social support,and strengthen social support helps to improve the old man's subjective sense of happiness.

10.3969/j.issn.2095-4344.2013.26.011

Objective:To study the anatomic and clinical experience in distally based neuroadipofascial turnover flap incorporating superficial peroneal nerve.Methods:Thirty six (32 preserved and 4 fresh) cadaver lower limbs were used to investigate the blood supply of superficial peroneal nerve and the relationship with the vascularization of its vicinity integument.Distally based neuroadipofascial flap incorporating the nutrifying vessels of superficial peroneal nerve was designed for dorsal foot reconstruction.Results:The proximal part of superficial peroneal nerve was supplied by an accompanying superficial peroneal artery.In the one third part of the lower leg,this artery became an interlacing paraneural vascular plexus and was reinforced by branches from the distal perforators of anterior tibial artery,the supramalleolar perforator of peroneal artery,and the recurrent branches of dorsal pedal artery.Two chain linked longitudinal vascular plexuses (fascial and paraneural) were confirmed in the anterior lower leg integument.In practice,distally based superficial peroneal neuroadipofascial turnover flaps were successfully transferred in 5 cases.The flap measured (10 14) cm?(4 5) cm in size.The pedicle was (3 4) cm in width.The pivot point was located (2 3) cm above the line between medial and lateral malleolus.Conclusion:Distally based superficial peroneal neuroadipofascial turnover flap is a simple and safe procedure with minimal morbidity of the donor site.

Objective:To explore effect of serum leptin level on nutriture in the patient of chronic renal failure(CRF)and the relation with TCM syndrome types of CRF.Methods:110 cases in CRF group were divided into 5 subtypes and 30 normal persons were used as control group.Serum leptin level,body height,body weight,and body mass index(BMI)were determined in both groups;and serum albumin,serum pre-albumin,transferin,serum creatinine,urea nitrogen,C-reaction protein,blood insulin,and nutrition risk index(NRI)were detected in CRF group.Results:Serum leptin level in CRF group was higher than that in the control group,and leptin level and nutritional index level in both the subtype groups of Qi-and Yang-deficiency of spleen and kidney were significantly different with those in other 3 subtype groups.Conclusion:High blood leptin is possibly one of the reasons inducing malnutriture of CRF patient,and serum leptin is possibly a partial basis of substances of Qi-and Yang-deficiency syndrome of CRF.

Objective To study the effect of vibration as a countermeasure against bone loss.Methods Volunteers were divided into vibration-group(VIB)and control-group(CON).Vibration experiments were performed in VIB for 3 months(10 min/d,5 d/week).Bone mineral density(BMD)of both VIB and CON was observed after 3 months.Results BMD in VIB increased obviously after vibration treatment(+1.29% for vertebrae and +1.65% for femur).The effectiveness rate was 80%.Conclusion Whole body vibration is effective in countermining bone loss.It has a broad prospect in treating osteoporosis and counteracting bone loss caused by weightlessness.

Objective To reduce the birthrate of ?-thalassemia major and improve the quality of population. Methods 527 pregnant women and their spouses were screened at 12~26 weeks of gestation with hematological data to find out carriers of ?-thalassemia. The blood samples of the couples who were both carriers were analyzed with PCR-RDB method for prenatal gene diagnosis. The umbilical blood samples were examined to prove the results. Results There were 28 cases of ?-thalassemia out of 1054 individuals, the positive rate was 2.66%; the ratio of male to female was 1/1.15. As the result of prenatal gene diagnosis, among four fetuses at risk, one was completely normal while three were with ?-thalassemia major(one homozygous and two compound heterozygous) who were aborted within 2 weeks after prenatal gene diagnosis. Conclusions It suggests that the PCR-RDB assay is effective in preventing the birth of ?-thalassemia major and has clinical significance in improving the population quality.

To introduce a new modification to simplify the neuroprosthesis of sacral anterior root stimulated micturition for Chinese patients with spinal cord injury (SCI). Spinal cord was transected at T 10 in six dogs and they were randomly divided into 2 groups. In dogs of group I, bilateral L 7 ～S 3 posterior rhizotomy (complete de afferentation, SDAF) was performed. In animals of group II bilateral L 7 , S 2 , S 3 posterior rhizotomy plus bilateral S 1 anterior rhizotomy (partial de afferentation combined with partial de efferentation) were performed. Cystometrogram (CMG) was carried out weekly after the operations. For clinical studies, ten patients with complete SCI above cone were included. Intraoperative electrical stimulation of each individual S 2 ～S 4 roots was carried out and bladder pressure changes were recorded simultaneously. The results showed that the dogs in group II manifested a similar CMG to that of group I, indicating that a flaccid bladder with good compliance and storing function was resulted. For parasympathetic innervation to bladder detrusor in Chinese, it had been found that S 3 root was the most frequent (100%) and the most efficacious (52 2%) contributor, S 4 ranked the second frequent (90%) and a lesser but still significant efficacious (44 9%), and S 2 the least frequent (25%) and the minimal contributor (2 9%). It suggested that using one cable with two electrodes placed on the anterior roots of S 3 and S 4 bilaterally, combined with severance of S 3 and S 4 posterior roots as de afferentation, or plus division of anterior root of S 2 as de efferentation, could restore both voiding and storing function of the bladder in Chinese SCI patients. This new modification has the advantages of reducing the cost of neuroprosthesis and broadening of its indication