Animal SPECT is an important research approach for translating preclinical to clinical study.It has been widely applied in drug development and the researches of physiology and diseases in small animal models.With the rapid progresses of hardware technology and algorithm of image reconstruction,the systemic sensitivity,spatial resolution and quantitative accuracy of animal SPECT have been greatly improved.Animal SPECT has great advantages over animal PET with the feasibility of study,the convenience acquisition of radiopharmaceuticals and relative low cost.In a certain period,animal SPECT will still be a main approach for preclinical researches of molecular imaging.

Objective To explore the clinical value of digital radiography with dual-energy subtraction in diagnosis of coronary artery and valve calcification. Methods 920 patients(older than 70) was examined by GE Revolution XRD with chest dual-energy subtraction. The positive rate of calcification of 146 patients who were highly or generally suspected for coronary artery calcification in bone window's and high Kilovotor digital graph of dual-energy subtraction were compared with the result of 16 slices MSCT. Results 135 patients with highly suspected coronary artery calcification and 11 patients with generally suspected coronary artery calcification in bone window's digital graph were examined by 16 slices MSCT. In those 135 patients with highly suspected coronary artery calcification, 128 cases were proved to be positive with 16 slices MSCT. In those 11 patients with generally suspected coronary artery calcification, 3 cases were proved to be positive with 16 slices MSCT. In those 12 patients who were positive with coronary artery calcification in high Kilovotor graph, 10 patients were confirmed to have coronary artery calcification in MSCT. In those 134 patients who were negative with coronary artery calcification in DDR, 126 patients were confirmed to have coronary artery calcification in MSCT. Conclusion Dual-energy subtraction digital radiography can offer potentially important new information in the evaluation of coronary artery calcium.

Objective To evaluate the effect of 5? reductase inhibitor on vascularity of benign prostatic hyperplasia (BPH). Methods Sixty male adult rats were randomly divided into 4 groups (15 per group): normal control, BPH control group, the group of 5? reductase inhibitor treatment during hyperplasia and the group of treatment after hyperplasia. Immunohistochemistry combined with computer assisted image analysis system were performed to examine the expression of factor Ⅷ related antigen, PCNA and VEGF in the prostatic tissue for all rats. Results The microvessel density(MVD) and the expressions of PCNA and VEGF were much less in treatment during hyperplasia and treatment after hyperplusia than that in BPH control group( P

Objective To evaluate the technique of multi-slice spiral CT angiography and it's value in diagnosing cerebrovascular diseases.Methods 77 cases were studied with GE Discovery Ultra 16-slice spiral CT scanner.The raw data were then transferred to ADW4.1 workstation and reconstructed with Volume Rendering(VR) and Maxium intensity projection(MIP)and Multiplanner Reconstraction(MPR).Results In 77 cases,49 were aneurysms,19 were AVM,7 were vaso-occlusive,2 were Moya-moya disease.Cerebrovascular was dispayed clearly in VR MIP and MPR.The images acquired with VR were more colorful and spatial than MIP and MPR,but the diagnosis value of MIP and MPR were higher.Conclusion MSCTA is an available noninvasive method in diagnosing the cerebrovascular disease.It could define character and position.MSCTA is an effective method and can be an nalterntive of DSA partially.

Objective To summarize that the distant interval metastasis of nasopharyngeal carcinoma(NPC) patients who have been examined by 18F-FDG PET/CT and analysis the diagnostic efficacy of doctors using PET/CT device.Methods 75 patients were divided into three groups including primary patients without any treatment and patients in treating and patients after radiotherapied.The distant interval metastasis sits,incidence and their difference were analyzed.We adopt five grades to diagnose distant interval metastasis of NPC.And we choose the difference grade as cut off point to draw ROC curve and then decide the best diagnostic cut off point.Result There are 22patients in the first group and 2patients in the second group and 51patients in the third group in all 75paients.The incidence of distant interval metastasis in the first group is 59.1% and third 68.6% and there are statistical significance between groups(P=0.0001).The most frequent sites of distant interval metastasis is the distant interval lymph nodes(38.67%) and the second is skeleton(36%) and the third is liver(25.33%).ROC curve indicate that third one is the best cut off point because of doctors using PET/CT device can acquire the optimal sensitivity(86.36%)and specificity(88.24%) and accuracy(76.8%).Conclusion The incidence of distant interval metastasis is higher and the most frequent part is distant interval lymph nodes.And the doctors using PET/CT device diagnose the distant interval metastasis of NPC efficacy is higher.

Objective To examine the clinical factors associated with postoperative outcome in dementia combined with cerebral amyloid angiopathy-related intracerebral hemorrhage (CAA-ICH). Methods The clinical data of 25 surgical patients of dementia combined with CAA-ICH were retrospectively analyzed. The postoperative short-term effects and long-term effects were evaluated at the 30 d and 6 months after operation according to the modified Rankin score (MRS), and the influencing factors were analyzed. Results Among the 25 patients, eusemia was in 6 cases. Age ≥ 75 years, Glasgow coma score (GCS) ≤ 8 scores on admission, hypertension, postoperative anemia, pulmonary diseases, midline shift were the influencing factors of postoperative short-term effects (P < 0.05 or <0.01). Twenty-two patients were followed up for 6 months, and ensemia was in 9 cases. Age≥75 years, GCS ≤ 8 scores on admission, hypertension, postoperative anemia, hypoproteinemia and pulmonary diseases were the influencing factors of postoperative long-term effects (P<0.01 or<0.05). Conclusions Preoperative assessment is important for the patients of dementia combined with CAA-ICH. Age ≥ 75 years, GCS ≤ 8 socres on admission, hypertension, postoperative anemia, pulmonary diseases, midline shift are associated with poor short-term effects; age ≥ 75 years, GCS ≤ 8 scores on admission,hypertension, postoperative anemia, hypoproteinemia and pulmonary diseases are associated with poor long-term effects.

Objective To compare differences of gut microbiome between patients with severe acne vulgaris and healthy individuals by using high-throughput sequencing technology.Methods Stool samples were collected from 10 outpatients with severe acne vulgaris and 10 age-and sex-matched healthy controls.Then,the bacterial DNA was extracted and subjected to 16S rRNA sequencing for the identification of microbial species,and the differences of gut microbiome were compared between the patients and controls.Results There were no significant differences in the diversity of intestinal microflora,but only the relative abundance of a few bacteria differed significantly between the two groups.Gut microbiome in the two groups mainly consisted of Bacteroidetes,Firmicutes,Proteobacteria and Actinobacteria.There were no significant differences in the relative abundance of bacteria at the phylum and genus levels between the two groups.However,the relative abundance of Blautia producta and Coprococcus eutactus at the species level differed remarkably between the two groups.Conclusion No significant differences in the bacterial diversity indices are found,but some bacterial species significantly differ between the patients with severe acne vulgaris and healthy controls.

Objective To evaluate the diagnostic value of the SPM-based interhemispheric asymmetry analysis (SIAA) of 18F-FDG PET brain imaging in localization of temporal lobe epileptic foci.Methods A total of 73 patients (42 males,31 females,average age: (27.10±9.78) years) diagnosed as temporal lobe epilepsy (TLE) and 30 healthy volunteers (17 males,13 females,average age: (33.79±5.46) years) from March 2012 to July 2015 were enrolled in this retrospective study.18F-FDG PET brain imaging was performed on all subjects to localize temporal lobe epileptic foci.The results of 18F-FDG-PET were analyzed by SIAA,visual analysis and asymmetry index (AI) analysis.The accuracy of the three methods was evaluated according to the postoperative pathological results and curative effect.χ2 test and partition of χ2 test were used to analyze the data.Results The accuracies of localization with SIAA,visual analysis and AI analysis were 87.67％(64/73),75.34％(55/73) and 69.86％(51/73),respectively (χ2=6.993,P<0.05).The accuracy of SIAA was higher than that of visual analysis and AI analysis (χ2 values: 5.818 and 8.481,both P<0.017),while there was no significant difference between AI analysis and visual analysis (χ2=0.500,P>0.017).Conclusion The SIAA of 18F-FDG PET brain imaging may be a more objective and accurate method in localization of TLE foci.

Objective To evaluate the effects of preoperative prophylactic infusion of cephalosporins antibiotics on electrocardiogram(ECG)during anesthesia induction with propofol. Methods Fifty female patients,of American Society of Anesthesiologists physical status Ⅰ or Ⅱ,aged 20-50 yr,weighing 43-75kg,scheduled for elective surgery,were divided into cefuroxime sodium group(group CEFU)and cefathiamidine group(group CEFA),with 25 patients in each group. After cefuroxime sodium was infused in group CEFU or cefathiamidine was infused in group CEFA,propofol was given by target-controlled infusion(plasma concentration 4 μg/ml).Before infusion of cefuroxime sodium or cefathiamidine,after completion of infusion and at 5min after propofol reached the target concentration,12-lead ECG was recorded,and mean arterial pressure and heart rate were simultaneously recorded. Tp-e interval and Tp-e/QT ratio were calculated.Results Compared with the baseline before infusion of cefuroxime sodium or cefathiamidine,Tp-e interval was significantly prolonged after completion of infusion and at 5min after propofol reached the target concentration in group CEFU,and Tp-e interval was significantly prolonged,and Tp-e/QT ratio was enlarged in group CEFA(P<0.05).There was no significant difference between the two groups in mean arterial pressure and heart rate at each time point (P>0.05).Conclusion Preoperative prophylactic infusion of cefuroxime sodium produces less ECG interference than preoperative prophylactic infusion of cefathiamidine during anesthesia induction with propofol.

Objective To develop the dual-beam technology of cyclotron to produce radioactive nuclide, which can increase radiopharmaceuticals output. Methods A new target is installed in cyclotron standby target, and at the same time the tubes and electronic components are fixed to produce radioactive nuclide through dual-beam technology, the product is tested by the dose calibrator. Results The radioactive nuclide can be produced through dual-beam technology and the output is nearly double times about which a single target. Conclusion The productions of cyclotron radioactive isotope are improved by dual-beam technology and meet the clinical needs of PET/CT examination for drugs.