Animal SPECT is an important research approach for translating preclinical to clinical study.It has been widely applied in drug development and the researches of physiology and diseases in small animal models.With the rapid progresses of hardware technology and algorithm of image reconstruction,the systemic sensitivity,spatial resolution and quantitative accuracy of animal SPECT have been greatly improved.Animal SPECT has great advantages over animal PET with the feasibility of study,the convenience acquisition of radiopharmaceuticals and relative low cost.In a certain period,animal SPECT will still be a main approach for preclinical researches of molecular imaging.

Objective To explore the clinical value of digital radiography with dual-energy subtraction in diagnosis of coronary artery and valve calcification. Methods 920 patients(older than 70) was examined by GE Revolution XRD with chest dual-energy subtraction. The positive rate of calcification of 146 patients who were highly or generally suspected for coronary artery calcification in bone window's and high Kilovotor digital graph of dual-energy subtraction were compared with the result of 16 slices MSCT. Results 135 patients with highly suspected coronary artery calcification and 11 patients with generally suspected coronary artery calcification in bone window's digital graph were examined by 16 slices MSCT. In those 135 patients with highly suspected coronary artery calcification, 128 cases were proved to be positive with 16 slices MSCT. In those 11 patients with generally suspected coronary artery calcification, 3 cases were proved to be positive with 16 slices MSCT. In those 12 patients who were positive with coronary artery calcification in high Kilovotor graph, 10 patients were confirmed to have coronary artery calcification in MSCT. In those 134 patients who were negative with coronary artery calcification in DDR, 126 patients were confirmed to have coronary artery calcification in MSCT. Conclusion Dual-energy subtraction digital radiography can offer potentially important new information in the evaluation of coronary artery calcium.

Objective To evaluate the effect of 5? reductase inhibitor on vascularity of benign prostatic hyperplasia (BPH). Methods Sixty male adult rats were randomly divided into 4 groups (15 per group): normal control, BPH control group, the group of 5? reductase inhibitor treatment during hyperplasia and the group of treatment after hyperplasia. Immunohistochemistry combined with computer assisted image analysis system were performed to examine the expression of factor Ⅷ related antigen, PCNA and VEGF in the prostatic tissue for all rats. Results The microvessel density(MVD) and the expressions of PCNA and VEGF were much less in treatment during hyperplasia and treatment after hyperplusia than that in BPH control group( P

Objective To compare differences of gut microbiome between patients with severe acne vulgaris and healthy individuals by using high-throughput sequencing technology.Methods Stool samples were collected from 10 outpatients with severe acne vulgaris and 10 age-and sex-matched healthy controls.Then,the bacterial DNA was extracted and subjected to 16S rRNA sequencing for the identification of microbial species,and the differences of gut microbiome were compared between the patients and controls.Results There were no significant differences in the diversity of intestinal microflora,but only the relative abundance of a few bacteria differed significantly between the two groups.Gut microbiome in the two groups mainly consisted of Bacteroidetes,Firmicutes,Proteobacteria and Actinobacteria.There were no significant differences in the relative abundance of bacteria at the phylum and genus levels between the two groups.However,the relative abundance of Blautia producta and Coprococcus eutactus at the species level differed remarkably between the two groups.Conclusion No significant differences in the bacterial diversity indices are found,but some bacterial species significantly differ between the patients with severe acne vulgaris and healthy controls.

Objective To examine the clinical factors associated with postoperative outcome in dementia combined with cerebral amyloid angiopathy-related intracerebral hemorrhage (CAA-ICH). Methods The clinical data of 25 surgical patients of dementia combined with CAA-ICH were retrospectively analyzed. The postoperative short-term effects and long-term effects were evaluated at the 30 d and 6 months after operation according to the modified Rankin score (MRS), and the influencing factors were analyzed. Results Among the 25 patients, eusemia was in 6 cases. Age ≥ 75 years, Glasgow coma score (GCS) ≤ 8 scores on admission, hypertension, postoperative anemia, pulmonary diseases, midline shift were the influencing factors of postoperative short-term effects (P < 0.05 or <0.01). Twenty-two patients were followed up for 6 months, and ensemia was in 9 cases. Age≥75 years, GCS ≤ 8 scores on admission, hypertension, postoperative anemia, hypoproteinemia and pulmonary diseases were the influencing factors of postoperative long-term effects (P<0.01 or<0.05). Conclusions Preoperative assessment is important for the patients of dementia combined with CAA-ICH. Age ≥ 75 years, GCS ≤ 8 socres on admission, hypertension, postoperative anemia, pulmonary diseases, midline shift are associated with poor short-term effects; age ≥ 75 years, GCS ≤ 8 scores on admission,hypertension, postoperative anemia, hypoproteinemia and pulmonary diseases are associated with poor long-term effects.

Objective To evaluate the technique of multi-slice spiral CT angiography and it's value in diagnosing cerebrovascular diseases.Methods 77 cases were studied with GE Discovery Ultra 16-slice spiral CT scanner.The raw data were then transferred to ADW4.1 workstation and reconstructed with Volume Rendering(VR) and Maxium intensity projection(MIP)and Multiplanner Reconstraction(MPR).Results In 77 cases,49 were aneurysms,19 were AVM,7 were vaso-occlusive,2 were Moya-moya disease.Cerebrovascular was dispayed clearly in VR MIP and MPR.The images acquired with VR were more colorful and spatial than MIP and MPR,but the diagnosis value of MIP and MPR were higher.Conclusion MSCTA is an available noninvasive method in diagnosing the cerebrovascular disease.It could define character and position.MSCTA is an effective method and can be an nalterntive of DSA partially.

Objective To summarize that the distant interval metastasis of nasopharyngeal carcinoma(NPC) patients who have been examined by 18F-FDG PET/CT and analysis the diagnostic efficacy of doctors using PET/CT device.Methods 75 patients were divided into three groups including primary patients without any treatment and patients in treating and patients after radiotherapied.The distant interval metastasis sits,incidence and their difference were analyzed.We adopt five grades to diagnose distant interval metastasis of NPC.And we choose the difference grade as cut off point to draw ROC curve and then decide the best diagnostic cut off point.Result There are 22patients in the first group and 2patients in the second group and 51patients in the third group in all 75paients.The incidence of distant interval metastasis in the first group is 59.1% and third 68.6% and there are statistical significance between groups(P=0.0001).The most frequent sites of distant interval metastasis is the distant interval lymph nodes(38.67%) and the second is skeleton(36%) and the third is liver(25.33%).ROC curve indicate that third one is the best cut off point because of doctors using PET/CT device can acquire the optimal sensitivity(86.36%)and specificity(88.24%) and accuracy(76.8%).Conclusion The incidence of distant interval metastasis is higher and the most frequent part is distant interval lymph nodes.And the doctors using PET/CT device diagnose the distant interval metastasis of NPC efficacy is higher.

Objective To compare the antitumor effect of self-developed albumin bound paclitaxel for injection ( PAB) and commercial albumin-bound paclitaxel for injection ( Abraxane ) . Methods The antineoplastic effects of PAB and Abraxane were evaluated in H22, Lewis and RM-1 allograft tumor mouse models after repeated intravenous injection (13. 4, 20. 0, 30. 0 and 45. 0 mg·kg-1 PAB and 20. 0 and/or 30. 0 mg·kg-1 Abraxane, respectively). Results PAB significantly inhibited H22 tumor growth at from the doses of 13. 4, 20. 0, 30. 0, and 45. 0 mg·kg-1,and the antitumor effect of PAB at 20. 0 mg·kg-1 was not significantly different from Abraxane at 20. 0 mg·kg-1 . PAB dose-dependently inhibited Lewis and RM-1 tumor growth at the doses of 20. 0, 30. 0, and 45. 0 mg·kg-1 . The no observed adverse effect level of PAB and Abraxane was 20. 0 mg· kg-1 in Lewis and RM-1 bearing C57 female mice. The antitumor effect and toxicity was not significantly different between PAB and Abraxane at equivalent doses. Conclusion At the same dose level, the antitumor activity and toxicity of PAB was equivalent to those of Abraxane.

To investigate whether accelerated blood clearance (ABC) phenomenon could be induced after repeated injection of mitoxantrone thermosensitive liposomes, LC-MS/MS and enzyme linked immunosorbent assay (ELISA) were used to measure the concentration of mitoxantrone and the anti-polyethylene glycol (PEG) IgM levels in rat plasma, separately. The drug was rapidly cleared away after the second administration. The anti-PEG IgM was detected after the first dose which was neutralized quickly after the second dose. It is proved that repeated administration of mitoxantrone thermosensitive liposomes in rat caused the ABC phenomenon.

Objective To assess the left atrial early function in pilots with essential hypertension by strian rate imaging (SRI). Methods Twenty-five hypertensive pilots without left ventricular hypertrophy and expansion (LVN) were selected. Twenty-ifve healthy pilots were included as control group. At the left ventricular apical four-chamber view, three-chamber view, two-chamber view, the systolic, early and late diastolic peak strain rate (SRs, SRe, SRa) curves were acquired by SRI in the basal section, the middle section and the upper section of the wall of left atrium. The average strain rate (mSRs, mSRe, mSRa) was calculated. The LA maximal, minimal and pre-systolic volume (LAVmax , LAVmin and LAVp), LA passive ejection fraction (LAPEF), LA active ejection fraction (LAAEF) , left atrial stroke volume (LASV), E/A and E/E′ were calculated. Results The LAPEF, LAAEF, LASV, E/A, E/E′were (38.96±6.43)%, (61.11±5.86)%, (14.74±5.33)mm, 1.23±0.08, 8.63±1.77 in the control group, and were (34.18±5.72)%, (63.23±6.89)%, (10.12±3.23)mm, 1.04±0.26, 7.16±0.84 in the study group. Compared with the control group, signiifcant difference was detect in LAPEF, LAAEF, LASV, E/E′(t=4.840, 2.690, 4.801, 2.950, all P<0.05). No signiifcant difference was found in E/A between the study and control group (t=-1.424, P=0.178). There was signiifcant positive correlation between SRa and E/E′(r=0.632, P<0.05). Conclusion SRI could be applied in the evaluation of early left atrial function. In pilots with essential hypertension, the changes of strain rate in left atrial wall, which is prior to the modeling geometry of left ventricular, could be detected by SRI.