Myelodysplastic syndrome (MDS) is a group of origin of hematopoietic stem/progenitor cells of myeloid clonal disorders.Studies have shown that MDS patients are with varying degrees of abnormal iron metabolism in the course of diagnosis and supportive care.Iron overload can damage the body's iron balance.Excess free iron can sink in plasma and tissue,especially in the body's major organs such as heart,liver and endocrine gland,resulting in structural damage and organ dysfunction,accelerating transformation to acute myeloid leukemia,and increasing the death rate.Iron chelation can improve the prognosis of patients,prolong overall survival and leukemia-free survival.The mechanism of iron overload in MDS research will be described in this paper.

Cancer stem cells are defined as the unique subpopulation in the tumors that possess the abili-ty to initiate tumor growth and sustain self-renewal as well as metastatic potential.Accumulating evidences in recent years strongly indicate the existence of cancer stem cells in solid tumors of a wide variety of organs.In recent years,many cancer stem cells have been found,including leukemia,breast cancer,brain ttunor,liv-er cancer,colon carcinoma,etc.In this review,we will discuss the conception and origin of the gastric cancer stem cells,and their role in the development of multi-drug resistance.

Objective To analysis the effect of vitamin, folic acid combined with Ginkgo biloba extract on cognitive function in elderly patients with stroke.Methods 56 patients who were diagnosed with acute cerebral infarction were collected.All patients were randomly divided into experimental group and control group, 28 cases in each group.Control group was given conventional treatment for improving circulation, nutrition brain cells, anti platelet aggregation, experimental group was given vitamin B6 tablets(20 mg,one time per day), folic acid tablets(20 mg, one time per day) and Ginkgo Biloba Extract Tablets(80mg, two times per day) on the basis of conventional treatment.After treatment, the levels of plasma Hcy, serum BDNF, UCP2 level and cognitive function were detected in all patients.ResuIts After treatment, compared with control group, the plasma Hcy level was significantly lower in experimental group(P<0.05).The serum BDNF level in experimental group was significantly higher (P<0.05) .The serum UCP2 level in experimental group was significantly higher (P<0.05) .The MMSE and MoCA scores of the patients in the experimental group were significantly higher (P<0.05).ConcIusions Vitamin, folic acid combined with Ginkgo biloba extract can reduce the level of plasma Hcy in elderly patients with stroke, improve serum BDNF, UCP2 levels, improve the cognitive function.

To study the clinical application of molecular diagnosis in cutaneous T-cell lymphoma (CTCL),the polymerase chain reaction Was used to detect the rearrangements of T-cell receptor (TCR)gene in 60 patients with cutaneous lymphocytic infiltrates.The result showed that dominant clonal TCR-? or ? gene rearrangements were detected in 36/40 CTCL,4/6 suspected mycosis fun- goldes/S?zary syndrome,1/1 lymphomatoid papulosis.No dominant clonal TCR-? or ? gene rearrange- ments were detected in 2 patients with CBCL,1 patient with lymphocytoma cutis,8 patient with benign lymphocytic infiltrates of skin and 2 normal controls.This study demonstrates that the method is help- ful for the lineage determination,clonality and early diagnosis of CTCL.

In order to investigate the type of infiltrating T cells in allergic contact dermatitis (ACD) reaction, by the methods of RT-PCR and immunohistochemistry, we tried to detect IL-2mRNA, IL-2, IFN-?, IL-4 in the lesions of patients with ACD. The results showed that Th1 cytokines were expressed aberrantly in ACD patients and Th1-like clone which secrets IL-2, IFN-? appeared to be predominant in ACD reaction.

Objective To evaluate the effect of duration of subcutaneous injection on the extent of bruising at injection sites among patients receiving low- molecular weight heparin. Methods Randomized clinical trials and quasi-experimental within-subject design that assessed the effect of subcutaneous duration on the bruising of injection sites were selected in Cochrane library, JBI evidence-based practice medical and nursing database, PubMed biomedical information retrieval platform, Medline general medical literature retrieval database. RevMan5.3 software was used to analyze the data after quality assessment, and data extraction were made for eligible trials. Results A total of 9 trials were included. The results of Meta-analysis supported the 30 s duration injection technique on reducing the rate of bruising of injection sites than 10 s duration injection technique (odds ratio was 0.34,95%confidence interval 0.25-0.47, P<0.01).While it was failed to support the effect on reducing the bruising area 48 and 72 hours after injection (P > 0.05). Conclusions Increasing the length of low-molecular weight heparin subcutaneous injection can reduce the rate of bruising. While it could not effectively reduce the area.

In order to meet the requirements of logistic supply in peacetime and wartime, General Health Department developed Managerial and Maintenance System of Army Medical Equipments, which is the important subject of 10th 5-year Army Medical Research. This system is based upon Basic Medical Equipments Distribution Standard, Army Medical Equipments Management System, joint support system and three-level maintenance network. An efficient platform for whole-survival-circle medical equipments monitoring is developed using computer information technology. This system can greatly advance the informatized, normative management of medical equipments, and thus is of avail in military and economic aspects.

Objective To investigate the main causes and strategies for the difficulties in ureteroscopic lithotripsy.Methods From March 2004 to December 2006,19 cases of ureteral calculi,who experienced difficulties during holmium laser lithotripsy or pneumatic lithotripsy under a rigid ureteroscope,were analyzed retrospectively.Among the cases,3 had difficulties in ureteroscope placement due to the narrow ureteral ingress,6 owing to calculus obstruction complicated with ureteral inflammatory polypi,4 resulted from twisted ureter,and 6 because of stenosis of the ureter.Results In 15 of the patients,the operation was successfully performed by changing surgical approach,controlling the hydraulic irrigation,and incising the stenotic segments,etc.Two patients,who had stenotic ureter,received ESWL with double-J catheter dwelling.Lithotripsy failed in 2 cases,and PCNL was used to remove the calculi.The postoperative complications occurred in 4 cases,including 2 with mucosal laceration and 2 ureteral perforations.One of the 4 cases was transferred to an open surgery,and the other 3 were cured by conservative treatments.Conclusions Holmium Laser lithotripsy and pneumatic lithotripsy under a rigid ureteroscope are safe and effective in treating ureteral calculi.

The baculoviral inhibitors of apoptosis play a significant role in infectivity and viral host-range, which make them potential candidates for the engineering and improvement of baculovirus insecticidal. The iap3 gene of Spodoptera exigua nucleopolyhedrovirus (SeMNPV), amplified by PCR, was 939 bp encoding IAP3. The PCR product was cloned into EcoR I/Bam H I of the plasmid pEGFP-C1. GFP was fused to the N-terminaus of IAP3 to study distribution in HEK293. It was observed that the plasmid expressing IAP3 significantly inhibited apoptosis induced by cisplatin in HEK293 cells. We conclude that the IAP3 of SeMNPV is functional in mammalian cells.

Tethered cord syndrome is a severe and congenital disease. Resecting the diseased ilium terminal(FT), eystis, lipoma is the main treatment for the disease, relieving the injury to the eonus medullaris. Why the FT is diseased and thickened? Is it necessary to eut internal FT or external FT? What is the injury mechanism of the low-positional or normal positional oonus medullaris? To study the component and structure of the conus medullaris and FT may give definite answers.