AIM: To study the fingerprint of Danggui Injection (Radix Angelicae Sinensis). METHODS: HPLC with UV detector was used in analyzing the patterns of fingerprint of Danggui Injection. It was separated on C 18 column. The mobile phase consisted of (A) water ( 0.25% HAC) and (B) methanol using a linear gradient, and the detection wavelength at 246nm. The flow rate was at 1mL?min -1 . RESULTS: The fingerprint of Danggui Injection was set up and the fingerprint of Angelica sinensis showed a correlation. CONCLUSION: The research is helpful to the quality control of Danggui Injection.