Objective To study the clinical significance and prognostic value of combined detection of urinary N?terminal telopeptide of type Ⅰ collagen ( NTx ) and serum C?terminal telopeptide of type Ⅰcollagen (ICTP) in breast cancer patients with bone metastases. Methods Serum and urine samples from 68 women with advanced breast cancer who received the treatment in Jilin Cancer Hospital between October 2012 and October 2008 were collected before and 2 months after treatment. Among them, 35 patients had bone metastases ( group A) , including 12 cases with single bone metastasis and 23 cases with multiple bone metastases. 33 out of the 68 patients had other organ metastases ( group B ) . 30 healthy females were included as the control group ( group C) . The levels of urine NTX and serum ICTP were measured by ELISA. Results The levels of NTx and ICTP of group A were significantly higher than those of groups B and C [NTx:(7.17±1.11) nmol/L vs. (2.08±0.22) nmol/L and (2.09±0.24) nmol/L, respectively, (P<0.05);ICTP:(7.75±1.14) ng/ml vs. (2.15±0.34) ng/ml and (2.22±0.31) ng/ml, respectively, (P<0.05) ] , while no significant difference was found between the groups B and C. Compared with the patients with single bone metastasis, the patients with ≥2 bone metastases had significantly higher NTx and ICTP levels [NTx:(7.69±0.93) nmol/L vs. (6.16±0.62) nmol/L, P<0.05; ICTP: (8.21±1.00) ng/ml) vs. (6.88±0.81) ng/ml (P<0.05)].At two months after treatment, 40.0%(14/35) of patients with bone metastasis had partial remission (PR), 42.9%(15/35) of patients had stable disease (SD), and 6 patients with progressive disease (PD). Both urinary NTx and serum ICTP of the patients with PR and SD were significantly decreased compared with those before treatment (P<0.05). 10 cases of patients (28.6%) with bone metastases had skeletal?related events (SREs) during the treatment, including one PR patient (1/14, 7.1%) , 5 SD patients ( 5/15, 33. 3%) and 4 PD patients ( 4/6, 66. 7%) . Conclusion The combined detection of urinary NTx and serum ICTP is of important significance for early diagnosis and efficacy evaluation and to control skeletal?related adverse events in breast cancer patients with bone metastases.

Objective To study the clinical significance and prognostic value of combined detection of urinary N?terminal telopeptide of type Ⅰ collagen ( NTx ) and serum C?terminal telopeptide of type Ⅰcollagen (ICTP) in breast cancer patients with bone metastases. Methods Serum and urine samples from 68 women with advanced breast cancer who received the treatment in Jilin Cancer Hospital between October 2012 and October 2008 were collected before and 2 months after treatment. Among them, 35 patients had bone metastases ( group A) , including 12 cases with single bone metastasis and 23 cases with multiple bone metastases. 33 out of the 68 patients had other organ metastases ( group B ) . 30 healthy females were included as the control group ( group C) . The levels of urine NTX and serum ICTP were measured by ELISA. Results The levels of NTx and ICTP of group A were significantly higher than those of groups B and C [NTx:(7.17±1.11) nmol/L vs. (2.08±0.22) nmol/L and (2.09±0.24) nmol/L, respectively, (P<0.05);ICTP:(7.75±1.14) ng/ml vs. (2.15±0.34) ng/ml and (2.22±0.31) ng/ml, respectively, (P<0.05) ] , while no significant difference was found between the groups B and C. Compared with the patients with single bone metastasis, the patients with ≥2 bone metastases had significantly higher NTx and ICTP levels [NTx:(7.69±0.93) nmol/L vs. (6.16±0.62) nmol/L, P<0.05; ICTP: (8.21±1.00) ng/ml) vs. (6.88±0.81) ng/ml (P<0.05)].At two months after treatment, 40.0%(14/35) of patients with bone metastasis had partial remission (PR), 42.9%(15/35) of patients had stable disease (SD), and 6 patients with progressive disease (PD). Both urinary NTx and serum ICTP of the patients with PR and SD were significantly decreased compared with those before treatment (P<0.05). 10 cases of patients (28.6%) with bone metastases had skeletal?related events (SREs) during the treatment, including one PR patient (1/14, 7.1%) , 5 SD patients ( 5/15, 33. 3%) and 4 PD patients ( 4/6, 66. 7%) . Conclusion The combined detection of urinary NTx and serum ICTP is of important significance for early diagnosis and efficacy evaluation and to control skeletal?related adverse events in breast cancer patients with bone metastases.

Objective To explore the integration of the digital imaging medical records system and the hospital information system ( HIS), for a hospital-wide information platform for digital medical records management. Methods All the medical records were classified as " to copy and not to copy" by sampling, and an item corresponding table was developed for both HIS medical record files and imaging records copying types. The imaging copying system for paper-based medical records only covers those must-copy ones, while those medical record files without need to be copied were directly transcoded via the interface to the imaging medical records system from the HIS system. This makes digital imaging medical records complete. Results The digital imaging medical record system is thus integrated, and the cost of making imaging medical records was sizably reduced without compromising the quality and integrity of medical records. Conclusions Imaging medical records produced by copying paper-based ones are integrated with those directly sent via the interface to the imaging medical records system, forming complete digital imaging records, at a much lower cost.

Obj ective To compare yhe effecys of ywo enyeral nuyriyion payhways in Geriayric ICU payienys receiving mechanical venyilayion as well as adopyion of effecyive care measures in order yo provide a basis for implemenyayion of enyeral nuyriyion feeding in yhese payienys.Methods A case-conyrol syudy was used.Fifyy ICU payienys receiving mechanical venyilayion were randomly divid-ed inyo nasogasyric yube group and nasoinyesyinal yube group,25 cases for each.Boyh group received proper nursing measures.The yime of achieving yargey dose of nuyriyion,nuyriyion improvemeny in 2,3,4 weeks and yhe incidence of complicayions were analysed.Results The yime of achieving yargey dose in nasoinyesyinal yube group was significanyly less yhan yhose in nasogasyric yube group (P<0.05).Afyer 4-weeks yreaymeny,yhe levels of yoyal proyein,serum prealbumin and yransfer-rin were improved in boyh groups wiyh a significanyly greayer degree in nasoinyesyinal yube group (P<0 .05 ).The incidence of complicayions in nasoinyesyinal yube group is significanyly less yhan yhose in nasogasyric yube group (P<0 .05 ).Conclusion Nasoinyesyinal yube feeding combined wiyh proper nursing measures in elderly ICU payienys receiving mechanical venyilayion makes yhe yargey dose of nuyriyion easily achieved and reduces yhe incidence of complicayions of mechanical venyilayion such as aspirayion and abdominal disyension.

Obj ective To compare yhe effecys of ywo enyeral nuyriyion payhways in Geriayric ICU payienys receiving mechanical venyilayion as well as adopyion of effecyive care measures in order yo provide a basis for implemenyayion of enyeral nuyriyion feeding in yhese payienys.Methods A case-conyrol syudy was used.Fifyy ICU payienys receiving mechanical venyilayion were randomly divid-ed inyo nasogasyric yube group and nasoinyesyinal yube group,25 cases for each.Boyh group received proper nursing measures.The yime of achieving yargey dose of nuyriyion,nuyriyion improvemeny in 2,3,4 weeks and yhe incidence of complicayions were analysed.Results The yime of achieving yargey dose in nasoinyesyinal yube group was significanyly less yhan yhose in nasogasyric yube group (P<0.05).Afyer 4-weeks yreaymeny,yhe levels of yoyal proyein,serum prealbumin and yransfer-rin were improved in boyh groups wiyh a significanyly greayer degree in nasoinyesyinal yube group (P<0 .05 ).The incidence of complicayions in nasoinyesyinal yube group is significanyly less yhan yhose in nasogasyric yube group (P<0 .05 ).Conclusion Nasoinyesyinal yube feeding combined wiyh proper nursing measures in elderly ICU payienys receiving mechanical venyilayion makes yhe yargey dose of nuyriyion easily achieved and reduces yhe incidence of complicayions of mechanical venyilayion such as aspirayion and abdominal disyension.

To construct, express and purify Exendin-4 analogue and detect its biological activity in vivo. Insert gene sequence into fusion partner ofpED plasmid which is helped to purification, entitled the new recombinant plasmid 5 Exendin-4 analogue polypeptide gene and fusion partner gene was linked by acid hydrolysisgene, transformed to E. coli BL21 and the fusion protein was induced by lactose. After acid hydrolysis, the Exendin-4 analogue polypeptide separated from fusion chaperon. Anion charge chromatography were used to further purification. 6 to 8 week-old ICR mice were injected (s.c) with Exendin-4 analogue, blood glucose and plasma insulin level was detected in different period after oral glucose tolerance test. The results show that high expression of inclusion body was induced by lactose, which accounted for 40% of germ proteins, the Exendin-4 analogue was obtained with the purity of 91.8% after being purified by anion charge chromatography. Bioactivity assay showed that the level of blood glucose of mouse which treated with exendin-4 analogue was obviously decreased to normal (P < 0.01), and the level of plasma insulin was increased obviously (P < 0.01).
Animals ; Cloning, Molecular ; Escherichia coli ; genetics ; metabolism ; Gene Transfer Techniques ; Hypoglycemic Agents ; metabolism ; pharmacology ; Insulin ; blood ; Male ; Mice ; Mice, Inbred ICR ; Peptides ; genetics ; pharmacology ; Recombinant Fusion Proteins ; biosynthesis ; genetics ; pharmacology ; Venoms ; biosynthesis ; genetics ; pharmacology

[Abstratc ] Objective To analyze whether there are differences in the efficacy and clinical outcomes to first-line tyrosine kinase inhibitors ( TKI) therapy in Chinese patients with metastatic non-small-cell lung cancer ( NSCLC) harboring different subtypes of epidermal growth factor receptor ( EGFR ) mutations.Methods A retrospective analysis was made on the clinical data of stage ⅢB or Ⅳ NSCLC patients who were diagnosed by histology and received EGFR mutation test, in order to confirm if there is any difference between the therapeutic effects of TKIs as first-line therapy and the prognosis.Results A total of 165 patients harboring EGFR exon 19 deletion (19del, n=71), exon 21 L858R mutation (L858R, n=80) or uncommon sensitive mutation ( n =14 ) were treated with EGFR-TKIs for first-line treatment.The comparison among different groups of common types of sensitive mutations revealed that the objective response rate ( ORR) of group 19del and group L858R were 57.8%and 45.0%, respectively ( P=0.113) . The disease control rate ( DCR) was 93.0%and 93.8%, respectively ( P=0.158) .However, the ORR and DCR of uncommon sensitive mutation were 35.7%and 78.6%, which were significantly lower than that of the group 19del (P=0.035) and group L858R (P=00.20 ).The median progression-free survival (PFS) of group 19del, group L858R and uncommon sensitive mutation were 14.0 months, 7.8 months and 5.1 months, respectively ( P=0.001) .The median PFS of the group 19del was significantly longer than that of the group L858R (P=0.009).The median overall survival (OS) of these three groups had significant difference (22.8, 15.2 and 10.0 months) ( P=0.048) .But those of group 19del and group L858R were similar ( P=0.152) . The multivariate analysis indicated that ECOG-PS (P=0.030), cigarette smoking (P=0.013) and EGFR mutation types (P=0.034) are independent prognostic factors of OS.Conclusions For Chinese NSCLC patients with different types of sensitive mutation, there are differences between their efficacy and prognosis of EGFR-TKIs as first-line treatment.The PFS of group 19del is obviously longer than that of other types of sensitive mutations, but have no significant differences in OS.The PFS and OS of patients with common types of sensitive mutation are better than those with uncommon sensitive mutation.

[Abstratc ] Objective To analyze whether there are differences in the efficacy and clinical outcomes to first-line tyrosine kinase inhibitors ( TKI) therapy in Chinese patients with metastatic non-small-cell lung cancer ( NSCLC) harboring different subtypes of epidermal growth factor receptor ( EGFR ) mutations.Methods A retrospective analysis was made on the clinical data of stage ⅢB or Ⅳ NSCLC patients who were diagnosed by histology and received EGFR mutation test, in order to confirm if there is any difference between the therapeutic effects of TKIs as first-line therapy and the prognosis.Results A total of 165 patients harboring EGFR exon 19 deletion (19del, n=71), exon 21 L858R mutation (L858R, n=80) or uncommon sensitive mutation ( n =14 ) were treated with EGFR-TKIs for first-line treatment.The comparison among different groups of common types of sensitive mutations revealed that the objective response rate ( ORR) of group 19del and group L858R were 57.8%and 45.0%, respectively ( P=0.113) . The disease control rate ( DCR) was 93.0%and 93.8%, respectively ( P=0.158) .However, the ORR and DCR of uncommon sensitive mutation were 35.7%and 78.6%, which were significantly lower than that of the group 19del (P=0.035) and group L858R (P=00.20 ).The median progression-free survival (PFS) of group 19del, group L858R and uncommon sensitive mutation were 14.0 months, 7.8 months and 5.1 months, respectively ( P=0.001) .The median PFS of the group 19del was significantly longer than that of the group L858R (P=0.009).The median overall survival (OS) of these three groups had significant difference (22.8, 15.2 and 10.0 months) ( P=0.048) .But those of group 19del and group L858R were similar ( P=0.152) . The multivariate analysis indicated that ECOG-PS (P=0.030), cigarette smoking (P=0.013) and EGFR mutation types (P=0.034) are independent prognostic factors of OS.Conclusions For Chinese NSCLC patients with different types of sensitive mutation, there are differences between their efficacy and prognosis of EGFR-TKIs as first-line treatment.The PFS of group 19del is obviously longer than that of other types of sensitive mutations, but have no significant differences in OS.The PFS and OS of patients with common types of sensitive mutation are better than those with uncommon sensitive mutation.