OBJECTIVE:To discuss the developing strategy of pharmaceutical enterprises run by private citizens in China.METHODS:Based on China’s realities,the confronting problems in pharmaceutical enterprises run by private citizens in China were analyzed.RESULTS&CONCLUSION:The privately running enterprises should take advantages of flexible or?ganization to strengthen innovation capacity,to achieve scale management with capital operation and to fasten the establishment of mordern product circulation.

Objective To observe the efficacy of advanced non-small cell lung cancer patients treated with CIK cell combined with chemotherapy.Methods65 cases of advanced lung cancer patients admitted in our hospital from February 2013 to January 2015 were retrospectively analyzed.The patients were randomly divided into observation group (34 cases) and control group (n=31).The levels of vascular endothelial growth factor (VEGF), matrix metalloproteinase-9 (MMP-9) and carcinoembryonic antigen (CEA) in the two groups were observed before and after treatment.The patients in the control group were treated with chemotherapy on the basis of chemotherapy.Cytokeratin 19 (CYFRA21-1) levels, and clinical efficacy.Results①The patients with VEGF and MMP-9 values after treatment (298.67±36.49)ng/L, (1102.31±86.48)ng/mL, than the control group (459.19±45.28)ng/L, (1394.83±121.06)ng/mL, and the difference was significant (P<0.05).②CEA and CYFR21-1 value patients in the observation group were (14.16±1.08) ng/mL, (4.45±0.45) ng/mL, than the control group (17.86±1.84) ng/mL, (5.76±0.64) ng/mL, and the difference was significant (P<0.05).③The total observation group was significantly higher than patients 94.18% 74.19% efficiency, and the difference was statistically significant (P<0.05).ConclusionCIK cell biological therapy combined with chemotherapy for advanced lung cancer patients with a certain improvement is worthy of further research and application.

Objective To analyse clinical efficacy of Fangfengtongsheng powder combined with Flupentixol and Melitracen tablets in treatment of anorectal neurosis.Methods92 patients with anorectal neurosis from April 2014 to June 2016 were grouped two groups randomly, each with 46 cases.Two groups were treated with Flupentixol and Melitracen tablets and observation group was treated another with Fangfengtongsheng powder.Clinical efficacy of two groups were compared.Results①Total effective rate of observation group was 95.65%,higher than that of control group 78.26% (P<0.05).②After treatment,SAS and SDS score of observation group were lower than that of control group(P<0.05).③After treatment, anal bulge, anal pain, frequent defecation, anal foreign body sensation score and 8 dimensions of SF-36 scale score of observation group were better than that of control group(P<0.05).④The control group had 8.70% adverse reactions, compared with observation group 4.35%, the difference was not statistically significant.ConclusionFangfengtongsheng powder can improve the therapeutic effect of anorectal neurosis on the basis of Flupentixol and Melitracen tablets, relieve the anxiety level and improve the living standard of patients.

OBJECTIVE:To provide information for future development of pharmaceutical industry.METHODS:The pay output rates of pharmaceutical industry were compared between China and America as well as Japan.RESULTS:The present situation and relative position of pay output in Chinese pharmaceutical industry were made clear.CONCLUSION:According to the pay comparative analysis result,we should raise technology structure,product structure and industry structure.On the basis of that,we can get long-term advantage.

Objective:To investigate effect of curcumin on serum gelatinases level in patients with unstable angina pectoris (UAP). Methods:A total of 80 UAP patients admitted from January 2010 to September 2010 were enrolled and randomly divided into curcumin treatment group (n=40) and routine treatment control group (n=40). Serum levels of gelatinases [contain matrix metalloproteinase (MMP)-2 and -9] of the two groups were measured before and 30d after treatment.Results: Compared with before treatment , after treatment, serum levels of gelatinases significantly decreased [MMP-2:(52.64±6.77)ng/ml vs.(32.65±1.67)ng/ml,MMP-9:(56.75±7.34)ng/ml vs.(35±1.88)ng/ml]in curcumin treatment group (P<0.01), and they were significantly lower than those of routine treatment control group[MMP-2:(32.65±1.67)ng/ml vs.(37.78±2.76)ng/ml,MMP9:(35±1.88)ng/ml vs.(40.23±1.95)ng/ml]],P<0.05. Conclusion:Curcumin could decrease serum levels of gelatinases in patients with unstable angina pectoris, and possesses effect stabilizing coronary artery plaque.

Ischemic cardiomyopathy (ICM) is due to coronary atherosclerosis cause long-term myocardial ischemia, make myocardial fibrosis diffuse, produce similar clinical syndrome like idiopathic dilated cardiomyopathy, which is one of a common clinical disease and causes increasing hazards to human health.Timely recovery of blood perfusion and coronary artery recanalization is one of the effective methods of treatment.However, it brings new damage while tissues and organs receive reperfusion, which is myocardial ischemia-reperfusion injury.In this paper, the current research progress in medicine myocardial ischemia-reperfusion injury and how to build a simple and efficient experimental animal models to mimic human myocardial ischemia-reperfusion injury were reviewed.

BACKGROUND: Atrial natriuretic peptide (ANP)-synthesizing cells distribute in all over the body. However, very little is known about the morphological localization and the distribution characteristics of ANP-synthesizing cells in different regions of rat stomach. OBJECTIVE: To investigate the ultrastructural localization and distribution characteristics of ANP-synthesizing cells in rat stomach. DESIGN: Repeated measurement experiment. SETTING: Chengde Medical Collage, Chengde, Hebei Province, China. MATERIALS: This study was performed at the Laboratory for Institute of Chinese Materia Medica Immunology (provincial key laboratory), Chengde Medical College between October 2004 and July 2007. Eighteen adult Wistar rats of either gender, weighing 175-300 g, were provided by the Laboratory Animal Center of Chengde Medical College. The disposal of animals was conducted in accordance with ethical guidelines for the use and care of animals. ANP antibody and serum (Santa Cruz Biotechnology, Inc, USA), Protein A-15 nm colloidal gold labeled (Sino-American Biotechnology Company, China), CMIAS image analysis system (Beijing University of Aeronautics and Astronautics, China) and JEM-1200EX transmission election microscope (Japan) were used in the study. METHODS: After gastric and right atrial tissues (as a positive control in immanohistochemical staining for ANP-synthesizing cells) were fleshly excised from anesthetized Wistar rats, the specimens were longitudinally harvested along rat gastric cardiac region, gastric fundic mucosa and gastric pyloric region. Gastric tissue was performed immunohistochemical staining (for positive control) together with right atrial tissue, to observe the distribution characteristics of ANP-synthesizing cells in different regions of rat gastric tissue, and to localize ANP-synthesizing cells. The ultrastructural localization of ANP-synthesizing cells in the rat gastric mucosa was performed by postembedding immunoelectron microscopy. The area-density percentage of ANP-synthesizing cells in the different regions of rat stomach (gastric cardiac region, pyloric region and fundic region) was calculated with a CMIAS image analysis system. MAIN OUTCOME MEASURES: Morphological appearance and localization of ANP-synthesizing cells as well as the different area-density percentage in different regions of rat stomach. RESULTS: As a positive control, ANP-synthesizing cells showed intense positive expression in atrial myocytes and cytoplasm and in gastric mucosa (mainly within the lower third of rat gastric mucosa). The morphological appearance of the individual ANP-synthesizing cell was variable, exhibiting round, pyramidal and flask shapes. Negative staining for ANP-synthesizing cells was detected in the lamina propria, submucosa, and smooth muscle. ANP-synthesizing cells existed in different regions of rat stomach and its area-density was the largest in the pyloric region. The distribution order of ANP-synthesizing cells in area-density was gastric cardiac region > gastric pyloric region > gastric fundic mucosa in mucosa layer. CONCLUSION: ANP-synthesizing cells mainly exist within the lower third of the rat gastric mucosa. The percentage of area-density of ANP-synthesizing cellswas variable in different regions of rat gastric mucosa, and its percentage of area-density was the largest in the gastric cardiac region.

Objective To study the application of the linear sampling method(LSM) in 2D electromagnetic inverse scattering imaging and to mainly discuss the imaging quality of LSM with partial-view data.Methods Get the norm of solution vector to the equivalent integral equation using numerical calculation in every sampling point at imaging region,and represent the norm with gray to reconstruct the scattering shape.Results Under all incident angles [0,360?] and all observation angles [0,360?],the imaging quality of the far-field pattern of scattering wave was obtained very well.But when reducing the range of the incident angles and observation angles,the imaging quality declined.Particularly,the imaging quality in the case of both incident angles and observation angles being at the same side of the observed object,were much better than both in opposite sides.Furthermore,the profile of the object was imaged more clearly as its position corresponding to the orientation of the incident/observation angle.Conclusion Although the imaging quality will decline with partial-view data in electromagnetic inverse scattering,the effectiveness of imaging with LSM still deserves attention.

Objective: To investigate effect of curcumin on serum gelatinases levels in patients with unstable angina pectoris（UAP）. Methods： A total of 80 UAP patients admitted from January 2010 to September 2010 were enrolled and randomly divided into curcumin treatment group (n=40) and routine treatment control group (n=40). Serum levels of gelatinases [contain matrix metalloproteinase（MMP）-2 and -9) of the two groups were measured before and 30d after treatment. Results：Compared with before treatment , after treatment, serum gelatinases levels significantly decreased [MMP-2：（52.64±6.77）ng/ml vs. （32.65±1.67）ng/ml，MMP-9：（56.75±7.34）ng/ml vs.（35±1.88）ng/ml ] (P<0.01) in curcumin treatment group (P<0.01), and they were significantly lower than those of routine treatment control group [MMP-2：（32.65±1.67）ng/ml vs.（37.78±2.76）ng/ml，MMP-9：（35±1.88）ng/ml vs.（40.23±1.95）ng/ml ] , P<0.05 all. Conclusion：Curcumin could decrease serum levels of gelatinases in patients with unstable angina pectoris, and possesses effect of stabilization coronary artery plaque.

Objective To investigate the energy metabolism state and the posttransplantive function under different warm ischemia time (WIT). Methods The left lobe of pancreas was resected, and according to WIT (30, 60, 90, 120 min), the canines were randomized into 4 groups. The graft was perfused and preserved by the UW solution (0-4 ℃) cold storage method for 24 h. And then the heterotopic segmental autotransplantation of pancreas was performed, while the normal control group and no warm ischemia(NWI)group were also set up. Blood sugar (BG) and intravenous glucose tolerance test (IVGTT) K value and the plasma insulin level were examined in the first week after pancreas transplantation. The amylase level of pancreatic juice 24 h and one week after transplantation were detected either. The tissue concentrations of adenosine triphosphate (ATP) and total adenine nucleotides (TAN) were determined using high performance liquid chromatography (HPLC). The pancreas cortex was taken for morphological analysis and ultrastructure study 1 h after transplantation. Results Plasma glucose in groups of 30 min and 60 min was returned to normal 3-5 days after transplantation, while groups of 90 min and 120 min could not one week after transplantation. IVGTT K values in groups of NWI, 30 min and 60 min were more than 1, while those in groups of 90 min and 120 min were less than 1 one week after transplantation. The plasma insulin levels in groups of 90 min and 120 min were much lower than those in groups of NWI, 30 min and 60 min (P