Objective:To explore the effect of verapamil on the activition of ?1(Ⅰ) collagen gene promotor induced by TGF ?1. Methods: The recombinant pCOLH 1.5 and pCOLH2.5 of ?1(Ⅰ) collagen gene promotor were transfected to rat mesangial cell and were treated with different concentration of TGF ?1 and verapamil. The activity of CAT were measured by ELISA method. Results:TGF ?1 enhanced the activity of pCOLH1.5 and pCOLH2.5(1.498 and 1.551 fold,respectively) significantly compared to the control; Verapamil inhibited the activity of the 2 recombinants. The activity of CAT stimulated by verapamil + TGF ?1 was remarkably decreased compared with the activity of pCOLH2.5 promotor by TGF ?1. Conclusion:Verapamil can not only inhibit the activity of gene promotor derectly, but also partly block the activity of ?1(Ⅰ) collagen gene promotor induced by TGF ?1.

Objective To investigate the expression of lentivirus-mediated short hairpin RNA (shRNA) targeting collagen typeⅠ (Col Ⅰ) in rat mesangial cells.MethodsThe Col Ⅰ shRNA lentivirus vector was constructed with three-plasmids packaging approach which encoding human/rat homology Col Ⅰ gene.Rat mesangial cells were infected by infection enhancer (control group),the empty lentiviral vector (pSC-GFP group),and lentiviral expression vector (pSC-GFP/ColⅠ group),respectively.Infection efficiency in the three groups was detected with flow cytometry,and the expression of Col Ⅰ mRNA and protein in the three groups was respectively detected by RT-PCR and Western blotting.ResultsRat mesangial cells were efficiently infected in pSC-GFP group and pSC-GFP/Col Ⅰ group.The infection efficiency was 72.42% in pSC-GFP group and 75.42% in pSC-GFP/Col Ⅰ group.RT-PCR showed that the expression of Col Ⅰ mRNA in pSC-GFP/Col Ⅰ group was significantly lower than that in control group and pSC-GFP group (P

Objective To reproduce an animal model simulating acute kidney injury caused high intensity military training in rats by forced exhausting running on the treadmill,that could be used for the investigation of problems arising from military training.Methods 40 male SD rats were randomly divided into three groups:control group(n=8),ordinary exercise group(n=16)and forced exercise group(n=16).The animals in ordinary exercise group were commonly fed.The animals in the other two groups were forced to take on one-time exhausting running exercises on the treadmill by different ways.The rats in forced exercise group were forced to run on the treadmill till exhaustion to reproduce the animal model of overtraining.The indexes of urine,serum and kidney tissues were observed and analyzed shortly after training and 24 hours later.Results After training,the values of urine protein,urinary albumin,urease,serum urea nitrogen,creatinine,angiotensin Ⅱ in serum and kidney tissues of the animals in forced exercise group increased obviously compared with those in control group and ordinary exercise group.Those indexes in ordinary exercise group were higher than in control group(P

Objective To study the effect of simulated intensive military training on the activity of the Na+,K+-ATPase in kidney of rats. Methods ① Twenty four rats were randomly divided into single bout of exhaustive exercise group(B) and control group(A).Rats in group B were forced to run on the treadmill until exhaustion according to the Bedford protocol,and their urine and the activity of Na+,K+-ATPase in kidney were tested immediately(B1,n=16) and 24 hours(B2,n=8) after exhaustion.② Another forty rats were randomly divided into 8-week incremental exercise group(D,n=20) and control group(C,n=20).Rats in group D were forced to run on the treadmill according to the incremental protocol for 8 weeks.The urine and the activity of Na+,K+-ATPase in kidney of group C and D were tested by the end of 4th week(C1 and D1) and by the end of 8th week(C2 and D2). Results ① As compared with group A,the values of urinary protein and urease in group B increased and the activity of Na+,K+-ATPase in kidney decreased obviously(P

Humans ; Hydrocarbons, Brominated ; analysis ; metabolism

Objective To explore the feasibility and value of low dose CT scan for the diagnosis of pulmonary tuberculosis.Methods 150 patients with pulmonary tuberculosis underwent three doses CT scan (standard dose:150 mA;low-dose:15 mA and 30 mA) using GE dual slices helical CT.Besides the different tube current,other scan parameters including tube voltage,scan cycle,pitch,and collimation were the same in three dose groups.The scanned extent was from apexes to bases of lung.Image quality in standard group was compared with that in other two low dose groups and analyzed statistically by three radiologists.Results CT characteristics of pulmonary tuberculosis could be detected efficiently using low dose CT scan(30 mA) program,which was no significant as compared with the CT image using standard CT sose(P＞0.05).Howere,CT scan at 15 mA obviously affected on the diagnosis for both active and inactive pulmonary tuberculosis,the difference was significant (P＜0.05).Conclusion Low dose CT scan can replace totally the standard dose CT scan in diagnosing pulmonary tubercrulosis.

Objective To establish a suitable animal model of experimental periodontitis and study the relationship between stress and inflammatory periodontal diseases.Methods Forty Wistar rats with nylon thread placed around the neck of maxillary left second molar tooth were divided randomly into two groups:stress group(the rats were treated with restraint strees for 12 h every day for 10 d)and control group.Four rats of either group were sacrificed at the day of 2,4,6,8,10,separately;the level of blood glucose and the contents of adrenocorticotropic hormone(ACTH),orticosterone and adrenaline were measured as the stress markers,as well as the relative weight of the thymus and spleen.The furcation area of the second maxillary molar was examined histologically and histometrically.Results In stress group,all the markers for stress were significant higher than those in control group,and the thymus and spleen were atrophied(P

Objective To study the estrogenic effect of organic extracts of effluent samples from different treatment stages in Tianjin reclaimed water treatment plant. Methods Reverse phase C-18 solid-phase extraction (RP-C18SPE) was used for the extraction of target compounds from water samples. Then, a simplified proliferation test with human estrogen receptor-positive MCF-7 breast cancer cell and recombined yeast assay were applied to assess the estrogenic effect in effluent samples from different treatment stages in Tianjin reclaimed water treatment plant. Results The amount of extracts of reclaimed water inducing the maximum effect in cell proliferation assay was equal to that from 1.00 ml primary water. In recombined yeast assay, the amount of extracts of reclaimed water inducing maximum ?-galactosidase activity was equal to that from 20.00 ml primary water. Conclusion The effluent of reclaimed water treatment plant still shows estrogenic effect though slightly reduced.

Objective To study the preventive and therapeutic effects of losartan and vitamin E on kidney injury induced by overtraining in a rat model. Methods Forty SD male rats were randomly divided into four groups (10 each): control group, model group, losartan group and vitamin E group. Rats in control group were fed with conventional diet. Rats in other three groups were forced to run on the treadmill for 8 weeks, once a day for 20 minutes and 5 days for each week, till exhaustion to induce the kidney injury. Rats in losartan group and vitamin E group were gavaged either with losartan or vitamin E everyday. Urine was collected at the end af each week, urine protein and N-acety-D-glucosaminidase (NAG) were detormined, and the serum urea nitrogen, creatinine (SCr), angiotensin Ⅱ, Na+-K+-ATP-ase activity in kidney were analyzed after 8 weeks' training. Results After 8 weeks' training, the urine protein, NAG in urine, blood urea nitrogen (BUN), SCr were obviously increased in model group, losartan group and vitamin E group as compared with that in control group, while those in model group were higher than that in losartan group and vitamin E group (P0.05). Na+-K+-ATP-ase activity of renal tissue were obvionsly lower in model group and losartan group than that in control group and vitamin E group (P

Objective To investigate the incidence of renal injury caused by strong intensity military training in the navy.Methods One thousand three hundred and twenty servicemen of the navy were enrolled in the present study including 568 recruits and 752 veterans.Urine protein and hemoglobin were determined using the dry chemistry method after a five-kilometer armed field race.Urine samples were collected and centrifuged for erythrocyte count under microscope after training.Retinol binding protein(RBP) and N-acetyl-?-D-glucosaminidase(NAG) were measured by enzyme linked immunosorbent assay(ELISA).Detection of creatine kinase(CK) above 950U/L was assumed to be rhabdomyolysis positive.Results The average incidence of hematuria,hemoglobinuria and proteinuria was 2.1%,7.4% and 44 %,respectively,and the incidence of abnormal RBP and NAG was 57.3% and 57.1%,respectively.Significant difference in each of the indexes existed between the recruits and veterans(P