Objective To explore the clinical diagnostic value of joint detection of serum platelet-derived growth factor BB (PDGF-BB), soluble vascular endothelial growth factor receptor -1 (sFlt-1) and urotensinⅡ( U-Ⅱ) in preeclampsia disease.Methods The cases of obstetric patients suffering from preeclampsia in the Third People′s Hospital of Liaocheng , Shandong Province between October 2012 and April 2014 were enrolled , including 96 cases of mild preeclampsia and 81 cases of severe preeclampsia.Totally 68 cases of normal pregnant women with similar age and gestational age were selected as control group.A case-control study was applied for the following investigations.The concentrations of serum PDGF-BB, sFlt-1 and U-Ⅱwere measured using ELISA.The diagnostic value of PDGF-BB, sFlt-1 and U-Ⅱalone or in combination for preeclampsia was analyzed and evaluated with receiver operating characteristic curve ( ROC) and Logistic regression analysis.Results Serum concentrations of PDGF-BB, sFlt-1 and U-Ⅱin mild preeclampsia group were (80.45 ±21.87)ng/L,(23.03 ±6.67)μg/L and(4.54 ± 1.02)ng/L, and those in severe preeclampsia group were (124.91 ±47.54)ng/L,(35.65 ±12.45)μg/L and(6.29 ±2.31) ng/L, while those in control group were (60.89 ±19.38) ng/L,(17.19 ±7.867)μg/L and ( 3.81 ±1.01 ) ng/L, respectively.The three parameters in mild preeclampsia group and severe preeclampsia group were significantly higher than those in control group ( P<0.01; F value was 79.43, 79.28 and 50.72 respectively ).In the same situation , these three indicators in severe preeclampsia group were significantly higher than those in mild group (P<0.01).Moreover, the AUC of serum PDGF-BB, sFlt-1 and U-Ⅱalone or in combination were 0.821, 0.786, 0.772 and 0.933, respectively.The differences between joint detection and three individual detections were statistically significant ( P<0.05 ) , as combined detection having a sensitivity of 95.7% and a specificity of 86.6%.Conclusion The combined detection of Serum PDGF-BB, sFlt-1 and U-Ⅱ had an important value in early assessment and treatment of preeclampsia.

Nano-biosensors have elicited considerable scientific interest in various research areas, including nanotechnology, bio-technology, microelectronics, and analytical techniques. The development of nanomaterials and nanotechnology has produced nano-bio-sensors with increasing potential applications in disease diagnosis. Prostate cancer is a malignant tumor that seriously threatens the health human males worldwide. Detecting the low-abundance biomarkers of prostate cancer is critical for its early diagnosis, treatment monitoring, and evaluation of its postsurgical recurrence. This review focuses on the research progress in nano-biosensing technology and its application in the detection of prostate cancer biomarkers.

Objective To approach the guide of ultrasound-guided minimally invasive biopsy technique in diagnosis and treatment of thyroid diseases. Methods One hundred and seventeen cases (a total of 131 nodules, nodules 0. 5-1.5 cm in diameter, with an average diameter of approximately 0. 8 cm) with thyroid nodules , under Ultrasound-guided minimally invasive biopsy techniques from October 2008 to July 2009 were analyzed. Result One hundred and seventeen cases of patients with biopsy derived satisfaction were not ap-parent discomfort and complications after biopsy. Across 59 cases of surgical treatment in patients with biop-sy, and preoperative biopsy and postoperative pathologic is not fully consistent in only two cases, 58 patients without surgical treatment, who is combined with symptoms, palpation, serum thyroxine and color Doppler ultrasound and other laboratory examinations, and regularly review treated with symptomatic treatment, the symptoms, and color Doppler ultrasound imaging were much better under the treatment. 8 cases of patients in 117 cases of minimally invasive biopsy have cervical lymph node biopsy at the same time, biopsy patholo-gy and postoperative results are consistent with postoperative pathologic (7 cases of metastatic carcinoma, 1 case of reactive hyperplasia), accuracy was 100%. Conclusion Uhrasound-guided minimally invasive bi-opsy technique have important guiding for diagnosis and treatment of thyroid diseases, consistent with the high rate of clinical, especially for the preoperative diagnosis of small nodules, to avoid unnecessary over-treatment, can also reduce the misdiagnosis of thyroid cancer. While this technology is simple, safe and ac-curate, which have clinical value in the thyroid surgery.

Objective To summarize the application of analogiczl acupoints,defined as a group of acu-points directed at the same disease,by data mining method on literature related to acupuncture and moxi-bustion treatment on dysmenorrheal in latest 10 years.Methods By the way of computer retrieval of Chinese Journal Full-Text database (CNKI)and Wanfang database (WF)and VIP database,a total 128 articles from 2010 to 2016 related to acupuncture and moxibustion treatment on dysmenorrheal were searched and sorted out.And the acupoints recorded were input into the computer and analyzed.Results There were 49 analogiczl points,of which 47 points at fourteen meridians and 2 extra points.The lead-ing three points were Sanyinjiao (SP 6),Guanyuan (CV 4)and Zhongji (RN 3);the leading meridians were bladder meridian of foot-Taiyang and Ren meridian;the location of the acupoints were almost loca-ted on thoracic abdomen and low back.According to the frequency,the analogiczl points were classified into three grades:9 of first-grade,13 of second-grade and 27 of third-grade.Conclusion The concept of “analogiczl acupoints”make a further interpretation of the synergetic effect of combination of acu-points,which would guide the selection of acupuncture prescription to improve the clinical effectiveness.

Objective: To explore the application influence of Maslow's theory of hierarchy of demands on the psychological and therapeutic compliance of patients with psychosis. Methods: Totally 100 patients with mental disorders who met the requirements of this study were selected from January 2016 to December 2016 in 984 Hospital of the Joint Logistics Support Force of the Chinese People's Liberation Army. They were randomly divided into 2 groups according to the number table method, 50 cases in each group. The control group adopted the routine nursing mode, while the experimental group followed the intervention mode guided by Maslow's hierarchy of needs theory, and implemented the corresponding nursing and health education. Concise Psychiatric Rating Scale (BPRS) was used to assess the severity of psychiatric symptoms, Self-knowledge and Treatment Attitude Questionnaire (ITAQ) was used to assess patients' compliance with treatment, and Quality of Life Scale (SQLS) was used to assess patients' quality of life. Results: After intervention, the symptoms of anxiety, depression, lack of vitality, thinking disorder, activation, hostile suspicion score, and BPRS scores of the control group were (5.84±3.51), (6.24±3.05), (6.49±2.35), (3.72±1.42), (4.34±1.81), (26.14±6.21) points. After the intervention of the study group, they were (3.78±2.07), (4.11±2.28), (5.15±2.36), (3.02±1.23), (3.58±1.07), (23.08±5.95) points. The differences between the two groups were statistically significant (t=2.516-2.845, P<0.05 or 0.01). After the ITAQ score intervention, the control group and the study group were (10.85±3.41) and (12.34±3.89) points. The difference between the two groups was statistically significant (t=-2.037, P<0.05). After the intervention, the quality of life of the control group was compared with psychosocial, motivation/energy, symptom/adverse reaction score, and total score of SQLS (25.34±7.16), (18.69±9.09), (10.54±1.31), (24.00±10.31) points. The study group were (19.06±4.26), (14.75±8.89), (9.56±1.06), (19.36±9.11) points, and the differences between the two groups were statistically significant (t=2.191-5.330, P<0.05). Conclusion Maslow's hierarchy of needs theory can significantly improve mental symptoms, improve treatment compliance and quality of life in psychiatric patients, and is worthy of clinical application.

Health literacy has become a vibrant area of research, and the nutrition literacy and health literacy were closely related as well as a growing number of inquiries have focused on direct measures of nutrition literacy. In this paper, we summarized some features such as the structure, the content, the reliability, the validity and the application of the general nutrition literacy assessment tools and the health literacy assessment tools related to nutrition literacy, furthermore the evaluation contents were listed as the understanding capacity, numeracy capacity, household food measurement, food group, and basic nutrition knowledge. In addition, the briefly the nutrition information readability assessment tools were also summarized. The research results of nutritional literacy and its assessment tools would provide reference for the development of nutrition literacy assessment tools and evaluation work in China.

Objective: To understand levels of various foods and nutrients in school lunch based on digital platform and to provide reference for food preparation and serving. Methods: A total of 13 018 school lunch recipes in Binhai New Area of 96 schools in Tianjin from November 2020 to April 2021 were collected by using digital management platform for food safety and nutritional health.Food types including cereals and tubers, vegetables, fruits, livestock and poultry meat, fish and shrimp, eggs, milk and dairy products, legumes and their products/nuts and others energy, and nutrients including protein, fat, carbohydrate, calcium, iron, zinc, selenium, vitamin A, vitamin B 1, vitamin B 2, vitamin C and dietary fiber were evaluated. Results: The qualified rate of all kinds of food for students lunch from high to low were 96.8% (116.4 g) of livestock and poultry meat, 92.3% (179.5 g) of cereal and potato, 65.0% (170.6 g) of vegetables, 47.7% (21.4 g) of soybeans and their products/nuts, 33.4% (18.0 g) of eggs, 14.4% (8.5 g) of fish and shrimp, 14.1% (19.6 g) of fruits, 0.3% (35.4 g) of milk and dairy products. There were significant differences in the qualified rate of various food intake among different grades( P <0.05). The qualified rate of students lunch energy was 76.9%(932.6 kcal). The qualified rates of various nutrients from high to low were iron 96.9%(9.7 mg), zinc 96.8%(5.9 mg), protein 96.4%(43.8 g), carbohydrate 87.6%(130.8 g) and selenium 82.9%(23.5 μg), vitamin C 78.5%(48.8 mg), vitamin B 1 75.9%(0.5 mg), fat 74.3%(28.5 g), vitamin A 74.1%(327.1 μ g) vitamin B 2 49.9%(0.5 mg), dietary fiber 19.5%(5.9 g) and calcium 13.4%(246.1 mg). There were significant differences in the qualified rates of energy and nutrients among different grades( P <0.05). Conclusion The digital platform basically meets school lunch requirments on food types and nutrients, but still with problems regarding insufficient fish and shrimp, fruits, milk and dairy products, vitamin B 2, dietary fiber and calcium. It is suggested to optimize school lunch recipes or increase corresponding nutrients content in other meals.

Objective To explore the protective effects of rutin against learning and memory impairment induced by trimethyltin (TMT) and investigate the possible mechanism. Methods Forty 6- to 9-week-old male BALB/c mice were randomized equally into saline group (control), TMT group, TMT+rutin group, and rutin group. Mouse models of learning and memory impairment were establish by acute TMT (2.25 mg/kg) exposure. In TMT+rutin and rutin treatment groups, the mice received intraperitioneal injection of rutin (10 mg/kg) for 1 week before TMT exposure. Twenty-four hours after TMT exposure, Morris water maze test was employed to test the escape latency of the mice, and the synaptophysin expression in the hippocampus and cortex were analyzed by Western blotting. Results Compared that in TMT group, the escape latency of the mice in water maze test was significantly shorter in the other 3 groups (P<0.05);the escape latency in TMT+rutin group was similar with that in the control and rutin groups (P>0.05). Western blotting showed significantly decreased synaptophysin expression in the hippocampus and cortex in TMT group (P<0.05); synaptophysin expression in TMT+rutin group increased significantly compared with that in TMT group (P<0.05) but showed no statistical significance from that in rutin and control groups (P>0.05). Conclusion Rutin pretreatment offers protective effect against TMT- induced learning and memory impairment in mice possibly by antagonizing decreased synaptophysin in the hippocampus and cortex.

Objective To explore the protective effects of rutin against learning and memory impairment induced by trimethyltin (TMT) and investigate the possible mechanism. Methods Forty 6- to 9-week-old male BALB/c mice were randomized equally into saline group (control), TMT group, TMT+rutin group, and rutin group. Mouse models of learning and memory impairment were establish by acute TMT (2.25 mg/kg) exposure. In TMT+rutin and rutin treatment groups, the mice received intraperitioneal injection of rutin (10 mg/kg) for 1 week before TMT exposure. Twenty-four hours after TMT exposure, Morris water maze test was employed to test the escape latency of the mice, and the synaptophysin expression in the hippocampus and cortex were analyzed by Western blotting. Results Compared that in TMT group, the escape latency of the mice in water maze test was significantly shorter in the other 3 groups (P<0.05);the escape latency in TMT+rutin group was similar with that in the control and rutin groups (P>0.05). Western blotting showed significantly decreased synaptophysin expression in the hippocampus and cortex in TMT group (P<0.05); synaptophysin expression in TMT+rutin group increased significantly compared with that in TMT group (P<0.05) but showed no statistical significance from that in rutin and control groups (P>0.05). Conclusion Rutin pretreatment offers protective effect against TMT- induced learning and memory impairment in mice possibly by antagonizing decreased synaptophysin in the hippocampus and cortex.

Objective To investigate the image features,categories,and clinical significances of Pneumoconiosis Ⅲ with large shadow.Methods The research is based on the analysis of image features of 168 pneumoconiosis Ⅲ patients who are directly diagnosed by qualified medical professionals in our institution.These image features are large shadows bigger than 2 cm×1 cm which can be observed by high kilovolt chest radiographs,DR chest radiography and chest CT scan.Results Large shadows bigger than 2 ×1 cm show noticeable imaging characteristics,like distribution of superior and middle lung regions (95.2％),irregular shapes (97.6％),splayed or sausage like changes on both sides perpendicular with ribs (89.2％),small shadows of pneumoconiosis (98.8％),and convergence of large shadow to hilum or mediastinal (53.6％).CT scan is significantly better than DR radiography on detection rate of focal emphysema,focal cavities and swollen lymph node of mediastinal.Conclusion Combining the image features of high kilovolt chest radiographs,DR chest radiographs and CT scan are the main methods to identify the shadows,and thus direct diagnose pneumoconiosis Ⅲ;CT scan plays an important role in differential diagnosis.