The principle of temperature control in operating room air decontamination system and the main reasons with difficulties of regulating temperature in transformation period of spring and autumn are analyzed. The reconstructed approaches of circulating water line on surface cooler are adopted appropriately, not only to solve the question of long temperature decreasing response time in operating room but also to save substantially a lot of expenditures and reduce the running loss.

Objective To explore the effects of walking exercise on glycometabolism,dynamic blood pressure and the quality of life of patients with both hypertension and type 2 diabetes on the basis of conventional drug treatment.Methods Sixty-two patients with both hypertension and diabetes who could support taking walking exercise of more than 5,000 steps/d were randomly divided into a walking exercise group (32 cases) and a control group (30 cases).Both groups were given conventional drug treatment (including valsartan,acarbose and metformin).Those in the walking exercise group took more than 10,000 steps/d of aerobic exercise while the patients in the control group were just given normal community care.This continued for a period of 3 months.Fasting plasma glucose (FPG),glycated hemoglobin-A1C (HbA1c),fasting insulin (FINS),the homeostasis model of assessment for insulin resistence index (HOMA-IR),the homeostasis model of assessment for insulin sensitivity (HOMA-IS),dynamic blood pressure parameters and quality of life were observed.Results In the walking exercise group,the FPG,HbA1c,FINS,HOMA-IR,HOMA-IS,dynamic blood pressure and quality of life indicators were all significantly different after 3 months of daily walking exercise compared with either baseline or the control group.Conclusion Accompanied by conventional drug therapy,10,000 steps/d of walking exercise can improve the glucose metabolism,dynamic blood pressure and quality of life of patients suffering from mild hypertension and type 2 diabetes.

Objective Brain natriuretic peptide(BNP) is released from the cardiac ventricles in response to increased wall tension. The prognostic significance of blood brain natriuretic peptide in Chinese patients with cardiovascular disease has not been established. The purpose of this study was to investigate the value of brain natriuretic peptide for predictin g cardiac death within 1 month in Chinese patients with cardiovascular disease. Methods One hundred and seven inpatients with cardiovascular disease, whose blood brain natriuretic peptide concentration were measured within 1 - 3 days of admission, using triage BNP test, were divided into 2 groups: the survival and the non-survival, according to the results of 1was positively correlated with heart rate, left ventricular end-diastolic dimension, history of heart failure and old myocardial infarction (r=0.28, P=0. 000 4; r=0.49, P＜0. 000 5; r=0.39, P＜0. 000 5; r=Area under the curve of the receiver-operating-characteristic(ROC) of brain natriuretic peptide to predict cardiac death at 1 month in patients with cardiovascular disease was 0.89%, 95% confidence interval 0.79-0. 98, P＜0. 000 5; stepwise logistic regression analysis indicated that brain natriuretic peptide (≥755pg/mL) was the only independent predictor of cardiac death at 1 month in patients with cardiovascular disease (OR= 17.6, 95 % confidence interval, 8.7- 66.5, P＜0. 000 5 ). Conclusion Brain natriuretic peptide might predict cardiac death at 1 month in patients with cardiovascular disease.

Objective :To study a simplified method of isolation of rat hepatocytes and to observe the pro-cess of cell morphology in long-term culture. Methods :Rat hepatocytes were isolated by a single two-stepperfusion method. The yield and viability were assessed by trypan blue exclusion. [3-(4,5-dimethylthiazol-2-yl)2,5-diphenyl tetrazolium bromide] (MTT) was used to test the effect of serum concentration of newborn calf serum on the proliferation of hepatocytes. Hepatocytes were inoculated in the culture mediumconsisted of Williams' E supplemented with insulin,dexamethasone and 10％ new born calf serum. Themorphologic change of cultured hepatocytes was observed. Results:The average yield of hepatocytes was 2.26× 108 cells per rat, with an average viability of 95.6％. New born calf serum had strong biological activi-ty to stimulate the proliferation of hepatocytes and there was close-effect relationship followed by the in-crease of new born calf serum concentration. The rat hepatocytes can be cultured for 5～ 6 weeks withpreservation of normal morphologic appearance. Conclusion:The rat hepatocytes isolated by the abovemethod have high yields and viability and can be long-term cultured in vitro.

AIM: To study the protective effects of Naokangning (Rhizoma Chuanxiong, Radix salviae miltiorrhizae, Hirudo, etc.) against cerebral ischemia. METHODS: We observed the effect of Naokangning on mice's resistance to cerebral ischemia when bilateral common carotid arteries and vagus nerves were ligated and hypoxia under normal pressure and airtight circumstance; With the model of partial cerebral ischemia by blocking rats'middle cer ebral artery (MACO):the effects of Naokangning on the activity of superoxide dismutase (SOD) and creatine kinas e(CK), the content of malondialdehyde (MDA) and nitric oxide (NO) were measured. RESULTS: Naokangning significantly raised mice's ability of anti-cerebral ischemia and prolonged span of life in hypoxia, Moreover, it also obviously improved the activity of SOD, reduced content of MDA in cerebrum, content of NO and activity of CK in blood serum after ischemia. CONCLUSION: Naokangning could strikingly protect brain caused by cerebral ischemia.

Objective　To observe the characteristics and rules of craniocerebral injury resulting from a high explosive shell to provide the bases for treating explosive injury. Methods　A total of 36 sheep were distributed at the distance 6 to 48 m away from the explosive center and the shell was exploded electrically at 7 m above the earth. At the same time, the velocity of fragments and shock wave pressure were determined. Gross and pathological observations were performed after injury. Results　Among all sheep with fragment injury, craniocerebral injury was 32%. Their immediate death rate was 75% and all died 6 h later. The incidence rates of penetrating wound and blind wound were 75% and 25% respectively. Pollution of wound track was heavy. The percentage of head lost was 50% in sheep and 50% of injured animal suffered from comminuted fracture of skull base. Bleeding was found extensively on the surface of the cerebrum, even medulla oblongata was involved. Hemorrhage, edema, rupture of small blood vessels and degeneration of neuron were found at the regions 4 cm away from the wound tract with light microscopy. Combined blast injury was found and occurred most often in the abdomen and limbs, both accounting for 62.5%, and combined thoracic injury was the third, up to 50%. All the animals of craniocerebral injury combined with lung blast injury. Conclusion　High explosive shells destroy cranium badly and extensively. Many skulls are lost and the cranial base is readily fractured. The wound track is heavily polluted. Combined injury is more often occurred.

OBJECTIVE: To explore the expression of Smac(second mitochondria-derived activator of caspase)and survivin on the growth, development, recurrence and carcinogenesis of the sinonasal inverted papilloma(NIP). METHOD: Immunohistochemical method was used to detective the expression of Smac and survivin in 10 cases of (nasal cavity mucosae, NM), 45 cases of NIP. The NIP group including 25 cases of NIP without dysplasia, 11 cases of NIP with dysplasia, and 9 cases of NIP with malignant transformation to squamous cell carcinoma(SCC). RESULT: The intensity of the positive expression of Smac in NIP was lower than NM, the intensity of the positive expression decreased with the decreasing degree of histological differentiation. There was significantly difference between NIP without dysplasia and SCC. The expression of survivin was negative in the control group, the expression intensity of NIP was enhanced. The degree of histological differentiation was lower, the intensity of the positive expression was higher. The expression between NIP without dysplasia and SCC had significantly differences. Smac negatively correlated with survivin(rs = -0.403, P<0.01). CONCLUSION Smac and survivin were associated with the growth and carcinogenesis of NIP.
Adult ; Aged ; Female ; Humans ; Inhibitor of Apoptosis Proteins ; metabolism ; Intracellular Signaling Peptides and Proteins ; metabolism ; Male ; Middle Aged ; Mitochondrial Proteins ; metabolism ; Nasal Mucosa ; metabolism ; pathology ; Nose Neoplasms ; metabolism ; pathology ; Papilloma, Inverted ; metabolism ; pathology ; Survivin

Purpose/Significance In order to coordinate and promote the high-quality development of hospitals,medical consuma-bles refined supply processing distribution(SPD)based on operational data repository(ODR)is built.Through ODR,cross-depart-mental data collaboration is realized,and an intelligent operation management system for medical consumables is established.Method/Process Through informatization design and application scenario analysis,the intelligent operation management architecture and service mode of consumables are constructed with data governance as the starting point.Result/Conclusion The intelligent operation system of medical consumables based on ODR will consolidate the fine management results under the SPD mode,strengthen hospital risk control,and further improve the scientific and standardized level of hospital operation and management.

Objective To explore the effect of water extracts from Semen Astragali Complanati (Flatstem Milkvetch Seed,hereinafter“SAC”)on lipid metabolism in hyperlipidemia model rats with kidney-yang deficiency pattern,so as to try to discover the mechanism.Methods Female 10-week-old SD rats were randomly divided into six groups (each n =10):normal group,model group,estrogen group,SAC low-dose group,SAC mid-dose group and SAC high-dose group.The rat model was induced by bilateral ovariectomization combined with high-fat diet feeding.And rats of normal group and model group were in-tragastrically (i.g.)administered with saline,those of estrogen group were i.g.with estradiol valerate (0.2 mg/kg),and three SAC groups were i.g.with SAC extract at the dose of 34,68 and 136 mg/kg for consecutive 8 weeks.The body weight of rats were recorded,serum TC,TG,HDL-C and LDL-C were measured and serum E2 ,FSH and estrogen receptors (ERα,ERβ)mRNA in liver tissue were de-tected.Results Compared with the normal group,the body weight of rats of model group increased sig-nificantly after three weeks’high-fat feeding (P <0.001 );from Week 5 the level of total cholesterol (TC)and low density lipoprotein cholesterol(LDL-C)increased significantly (P <0.001 or P <0.01), and from Week 7 triglyceride(TG)and high density lipoprotein cholesterol(HDL-C)also did (P <0.001 or P <0.05);serum E2 significantly decreased (P <0.01),and follicle stimulating hormone(FSH)in-creased (P <0.05),the expression of ER-αmRNA in liver significantly decreased(P <0.05).High dose of SAC significantly reduced the serum level of TG and TC from Week 7 (P <0.05 or P <0.001), middle dose of SAC reduced the level of LDL-C and increased HDL-C significantly at the same time point (P <0.05,P <0.01),and a more week later,the low dose increased the serum level of HDL-C (P <0.05).Despite the levels of E2 and FSH of model rats showed no significant difference after treatment by SAC (P >0.05),the expression of ER-αmRNA in liver tissue increased in all SAC groups (P <0. 05).Conclusion The effect of regulating blood lipid metabolism of SAC had been proved.The poten-tial mechanism could be via upregulating the expression of estrogen receptors to amplify the signals trans-mitted by the receptors,which may make up the low estrogen level to direct at the disorder of lipid metab-olism during peri-menopausal period.

Objective: To evaluate the efficacy and safety of drug-coated balloons (DCB) for de novo large coronary vessels. Methods: One hundred and two patients were retrospectively enrolled in this study, there were 104 lesions with the reference lumen diameter of target vessel more than 2.8 mm and patients were treated with DCB in de novo lesions during May 2015 and July 2017 in our center. Coronary artery angiography and quantitative coronary angiography were performed in 82 (80.4%) patients at follow up period ((8.1±1.7) months post procedure). The endpoints were late lumen loss (LLL) at follow up,and major adverse cardiac events (MACE) including cardiac death, myocardial infarction (MI), target lesion revascularization (TLR) and stent or target lesion thrombosis at 12 months post procedure. Results: Ninety-eight lesions were treated with DCB only, 6 (5.9%) bailout drug-eluting stent (DES) were used because of severe coronary dissection, 2 patients (2.0%) received revascularization driven by acute ischemic events during hospitalization. Cutting balloons and NSE balloons were used in 65.4% (68/104) and 26.0% (27/104) lesions. The lesion length was (12.57±3.58) mm and the DCB length was (19.87±4.55) mm. The late lumen loss was (0.01±0.52) mm during angiographic follow up. The TLR rate and overall MACE rate was 3.9% (4/102) and 3.9% (4/102) and there was no death,MI and target lesion thrombosis at 12 months follow up. Conclusion DCB treatment for de novo large coronary vessels is effective and safe.