1.Role of Neuroretinal Rim Volume / Rim Area ratio in Early Diagnosis of Primary Open Angle Glaucoma
Journal of Kunming Medical University 2006;0(06):-
Objective To explore the role of Neuroretinal Rim Volume/ Rim Area(RV/RA) ratio in Early Diagnosis of Primary Open Angle Glaucoma(POAG).Methods All candidates were divided in to three groups(31~40 years old,41~50 years old,51~60 years old),Heileberg Retina Tomography(HRT)were used to test retinal disk,and the RV/RA ratio were calculated.Result There are obviously difference between nomal and POAG group in RV/RA(P
2.The Researching Progress on Mechanism of Anti-tumor Action of Emodin
International Journal of Traditional Chinese Medicine 2008;30(3):228-230
Emodin has been known for its various pharmacological functions, including anti-rumor, anti-inflanmation,the inhibition of cell proliferation and secretion of pancreatic enzyme, acceleration of osteoblast cell differentiation and fibrinolysis, and promotion of wound healing. The researching progress of anti-tumor function of emodin was reviewed in this paper for the purpose of understanding it's mechanism of anti-tumor comprehensively.
3.Clinical Efficacy of Danshen and Chuanxiongqin Injection for Frequent Attacks of Transient Cerebral Ischemia: Observation of 40 Cases
Bing ZHANG ; Yan WANG ; Chunguang LIU
China Pharmacy 1991;0(06):-
OBJECTIVE:To observe the clinical curative effect of Danshen and Chuanxiongqin injetion for frequent attacks of transient cerebral ischemia. METHODS: A total of 78 patients with frequent attacks of transient cerebral ischemia were enrolled:40(treatment group) were assigned to receive Danshen and Chuanxiongqin injection 10 mL with 0.9% physionlogical saline 200 mL iv gtt q.d, 38 (control group) to receive Xuesaitong injection 10 mL with 0.9% physiological saline 200 mL iv gtt q.d for 14 days(for both group).RESULTS: There were significant differences between the treatment group and the control group in effective rate (95.00% vs.57.89% respectively) (P
4.Application of a Self Designed Retractor Made by Stainless Steel Tongue Depressor in Small Incision Appendectomy
Chunguang LI ; Peng YUAN ; Xuelai LIU
Chinese Journal of Minimally Invasive Surgery 2015;(6):548-550
[Summary] Between February 2010 and November 2014, a retractor fabricated by stainless steel tongue depressor was used in 30 cases of small incision appendectomy in our department .All the operations were performed via an incision 1.5-3 cm in length.No complications were found .Such self designed retractor can be clinically utilized with characteristics of cheapness , safety, and good exposure of operative field .
5.Metrology Measurement and Quality Analysis of Medical Oxygen Flowmeter
Huimei WANG ; Haibo LIU ; Chunguang YANG
Chinese Medical Equipment Journal 2003;0(10):-
Objective To check some medical oxygen flowmeters in use in the military area command. Methods According to JJG 917-1996 buoy oxygen inhalant measuring rules, 930 oxygen flowmeters in the military area command were measured. Results The unqualified reasons of medical oxygen flowmeter mainly lay in the capability decline of pressure resistance of humid bottle, the poor airproof of the interface, the overflow of pressure, and the overflow of flux value. Conclusion To improve the quality situation of medical oxygen flowmeter, the department of technical supervision, hospital and manufacturer need to make efforts together.
6.The surgical treatment of the chronic atlantoaxial rotatory dislocation with C1, 2 lateral facet locked through combined anterior and posterior approach
Qi CHEN ; Yueming SONG ; Limin LIU ; Xi YANG ; Chunguang ZHOU
Chinese Journal of Orthopaedics 2015;35(5):488-494
Objective To describe the clinical manifestation of the chronic atlantoaxial rotatory dislocation (AARD) with C1,2 lateral facets locked,and explore the effect of the operation combined with anterior retropharyngeal approach and posterior approach.Methods Data of 8 patients with chronic AARD with C1,2 lateral facet locked who had undergone open reduction with the anterior retropharyngeal approach and C 1,2 transpedicular screw fixation with autologous iliac bone graft from Oct.2010 to Jun.2013 were retrospectively analyzed.There were 4 males and 4 females with an average age of 31 years old (range,11-57 years old).The intervals from onset to diagnosis were from 29 to 180 days and the mean time was 70.6 days.5 cases were chronic AARD with right C1,2 lateral facets locked and 3 with left C1,2 lateral facets locked.Reduction was failed to obtain by traction for two to four weeks.Subsequently,after open reduction with the anterior retropharyngeal approach,the patients were performed C 1,2 transpedicular screw fixation with autologous iliac bone graft for one stage or two.Results 1 patient underwent open reduction with the anterior retropharyngeal approach in stage one and C1,2 transpedicular screw fixation in stage two because of an overall severe condition,and the other patients did anterior and posterior operation in one stage.The average operation time was 205 min (range,160-260 min).The mean blood loss was 210 ml (range,100-300 ml).There were no operation complications except one young girl reflected pain in autologous iliac donated area,and pain relieved when taking non-steroidal anti-inflammatory by oral for one week.All patients had been followed up for a mean period of 14.8 months (range,5-37 months).Three-dimensional computed tomography revealed C1,2 arthrodesis bone graft fusion from two to four months and the average was 3.1 months.Neither recurrence of symptoms nor dislocation was observed at the latest follow-up.Conclusion Open reduction through the anterior retropharyngeal approach with C1,2 transpedicular screw fixation is an effective treatment strategy for chronical AARD with C1,2 lateral facets locked,which reduces surgical complications with less operation difficulty.
7.Effects of Shenqi Compound Recipe on expression of COX-2 mRNA in aorta in GK rats
Min CHEN ; Hong GAO ; Chunguang XIE ; Ya LIU ; Can ZHUANG
China Journal of Traditional Chinese Medicine and Pharmacy 2006;0(09):-
Objective:To explore the effects of Shenqi Compound Recipe on the expression of Cycloxygenase-2 (COX-2)mRNA in aorta in GK rats.There were five groups:GK group,model group,atorvastatin group,Shenqi Compound Recipe group and normal control group.During the experiment periods,each group was administrated correspondent substance respectively for 35 days.Serum concentrations of C reactive protein(CRP)were determined by ELISA.The mRNA expressions of COX-2 in aorta were detemined by reverse transcriptase PCR(RT-PCR).Results:Compared with model group,concentrations of CRP in serum and the mRNA expression of COX-2 all decreased in atorvastatin group and Shenqi Compound Recipe group(P
8.Preparation of fluorescent polyclonal antibody against human IgG and its application in the diagnosis of nephrosis
Chunguang DUAN ; Yanfang LIU ; Shoujing YANG ; Caiyun WANG ; Lu YU
Journal of Medical Postgraduates 2003;0(12):-
Objective:To prepare polyclonal antibody against human immunoglobulin G(human IgG) and to use this antibody in the clinical diagnosis of nephrosis. Methods:New Zealand rabbit was immunized subcutaneously with human IgG.Enzyme linked immunosorbent assay(ELISA) was applied to reveal the titer of the prepared polyclonal antiserum against human IgG.Antiserum was purified with affinity chromatography,and the purified antibody was confirmed for its specificity by Western blot and immunohistochemical staining.The purified antbodies which have been indentified were fused with FITC(fluorescein isothiocyanate) and then analyzed by immunohistochemical staining.Finally the fused antibody was useed in the clinical diagnosis of nephrosis. Results: The titer of the obtained antiserum was up to(1∶128 000,) double agar diffusion test showed the titer of the antibody was 1∶32.By the method of affinity chromatography,we obtained purified antibody with the purity of 85%.ELISA,double agar diffusion and immunohistochemical staining tests showed that the specificity and titer of the antibody were not decreased sharply.The purified antibody fused with FITC also kept the specificity of the primitive antibody.When the FITC fused antibody was tried in nephrosis patients,it detected human IgG effectively. Conclusion:The polyclonal antibody can specifically recognize human IgG.This purified antibody fused with FITC can be used in the diagnosis of nephrosis.
9.Osteogenic differentiation of adipose-derived stem cells transfected by lentivirus vector carrying LIM mineralization protein-1 and hypoxia-inducible factor-1alpha
Weimin PAN ; Min LIU ; Jianchang YANG ; Chunguang DUAN ; Yue HUANG
Chinese Journal of Tissue Engineering Research 2015;(32):5140-5147
BACKGROUND:LIM mineralization protein-1 (LMP-1) and hypoxia-inducible factor-1α (HIF-1α) as intracelular proteins can induce osteogenic differentiation and promote angiogenesis, respectively. Therefore, their combination is of great significance for effectively inducing the osteogenic differentiation of adipose-derived stem cels.
OBJECTIVE:To study the osteogenic differentiation of adipose-derived stem cels transfected by lentivirus vector carrying LMP-1 and HIF-1α.
METHODS:Reverse transcription-PCR technology was employed to clone LMP-1 and HIF-1α genes, and thegenes were cloned to lentivirus vectors pLVX-EF1α-DsRed-Hyg and pLVX-EF1α-IRES2-AcGFP1 to construct main lentiviral vectors pLVX-EF1α-DsRed-Hyg-RLMP-1 and pLVX-EF1α-IRES2-AcGFP1-RHIF-1α. Then, Lenti-X 293T cels were transfected with main vectors pLVX-EF1α-DsRed-Hyg-RLMP-1 and pLVX-EF1α-IRES2-AcGFP1-RHIF-1α, packaging plasmid and coated plasmid. After that, lentiviral vectors were packaged to transfect adipose-derived stem cels from rats that were obtained by tissue explants culture and enzyme digestion methods. At 3, 7, 14 days after transfection, reverse transcription-PCR technology was adopted to detect the expression of osteogeic genes, such as bone morphogenetic protein 2, Runx-2, alkaline phosphatase, osteocalcin as wel as to detect the expression of vascular endothelial growth factor.
RESULTS AND CONCLUSION:Lentiviral vectors pLVX-EF1α-DsRed-Hyg-RLMP-1 and pLVX-EF1α-IRES2-AcGFP1-RHIF-1α were effectively transfected into adipose-derived stem cels. Reverse transcription-PCR results showed that from the 7th day to the 14th day after lentivirus transfection, bone morphogenetic protein 2, Runx-2, alkaline phosphatase and osteocalcin al over-expressed. These findings indicate that the combination of LMP-1 and HIF-1α can enhance the osteogenic activity of adipose-derived stem cels.
10.The diagnosis and treatment of duodenal polys and research reviews:14 cases
Chunguang GUO ; Qian LIU ; Chengfeng WAN ; Ping ZHAO
Clinical Medicine of China 2008;24(6):532-534
Objective To summarize the experience of diagnosis and treatment of the duodenal polys.Methods 14 patients of duodenal polys admitted between 1987 and 2006 were analyzed retrospectively.Results Clinical manifestations were melena,epigastric discomfort, abdominal pain,weight loss and abdominal distention.The ratio of correct diagnosis were 60% with duodenal endoscopy,56% with upper gastrointestinal radiography and 44% with CT.Most duodenal adenomas located at the second portion of the duodenum.8 duodenal adenomas turned into carcinoma and 2 cases recured in a year after operation.Conclusion Duodenal endoscopy and upper gastrointestinal radiography were effective for diagnosis of duodenal polys.Endoscopic ultrasound(EUS)helps to improve the diagnosis of the leions in submucosa.It should pay attention to the treatment of doudenal adenomas actively.The surgery remained the mainstay of the treatment.It needed attention for the screening and treatment of the duodenal adenomas.