1.The change of lymphocyte subpopulation of the thermal injured rats by exchange transfusion.
Ki Taek HAN ; Kyung Seul CHUNG ; Jung Gil CHUNG ; Sang Tae AHN ; Poong LIM
Journal of the Korean Society of Plastic and Reconstructive Surgeons 1992;19(3):327-337
No abstract available.
Animals
;
Lymphocyte Subsets*
;
Lymphocytes*
;
Rats*
2.Industrial Accidents in A Battery Manufacturing Industry.
Korean Journal of Preventive Medicine 1974;7(2):305-312
No abstract available.
Accidents, Occupational*
3.Four cases of edward syndrome with abnomal prenatal ultrasonographic findings.
Nam Gyu CHO ; Kyung Ik KWON ; Dong Ho NAM ; Chun Gun LIM ; Ho Chung RYU ; Jong In KIM ; Taek Hoon KIM
Korean Journal of Perinatology 1993;4(4):599-609
No abstract available.
4.Gait Analysis after Total Knee Arthroplasty.
Chin Youb CHUNG ; Sang Cheol SEONG ; Myung Chul LEE ; Young Wan MOON ; Tae Gyun KIM ; Soo Taek LIM
The Journal of the Korean Orthopaedic Association 1997;32(5):1290-1301
In order to identify the correlations between clinical results and quantitative data of gait analysis, we analyzed the results of 20 cases of total knee joint replacement arthroplasty in 15 patients with degenerative arthritis. We also evaluated the gait analysis of ten age-matched healthy candidates as a control group. Mean follow-up periods were 30 months. Clinical results included post-operative HSS (Hospital for Special Surgery) knee rating scores and the changes of the tibiofemoral angles. The three dimensional gait analysis included clinical assessment, video-taping, three dimensional kinematics and kinetics. The three dimensional kinematics were obtained using a 5 camera VICON system, and the three dimensional kinetic data was collected using two AMTI force plates. There was no statistical difference in linear parameters between the patient and control group. In patients group, however, double support time decreased as the HSS score increased, and range of knee motion and maximum knee flexion increased in accordance with the increase of pain score. Kinematic data of the patients group revealed that some parameters, such as knee flexion during loading response, knee flexion in swing phase, and knee varus during swing phase, were decreased. On the other hand, internal rotation of the knee from initial contact to initial swing was increased when compared with that of control group. There was no significant correlation between the degrees of tibiofemoral angle and coronal plane moment in the patients group. In three cases which showed mild varus instability post-operatively, knee flexion during loading response decreased and valgus moment in midstance increased as compared with the cases without instability. We believe that three dimensional gait analysis will be a good modality for evaluation of the results after total knee arthroplasty. With further accumulation of long term. follow-up data of gait analysis, we might be able to predict the long term results of total knee arthroplasty including possibility of loosening.
Arthroplasty*
;
Arthroplasty, Replacement
;
Biomechanical Phenomena
;
Follow-Up Studies
;
Gait*
;
Hand
;
Humans
;
Kinetics
;
Knee Joint
;
Knee*
;
Osteoarthritis
5.Disorder of Sex Development with 5alpha-reductase Deficiency in Identical Twins.
Sang Taek LEE ; Kihye SUNG ; Jung Lim BYUN ; Yeo Min YUN ; So Chung CHUNG
Journal of Korean Society of Pediatric Endocrinology 2008;13(2):184-187
Children with abnormal sex development may present with ambiguous genitalia in the newborn period or lacking of secondary sexual characteristics in puberty. Clinicians should make a prompt and accurate diagnosis and counsel parents on therapeutic options to minimize or avoid medical and psychological complications. 5alpha-reductase deficiency is a rare autosomal recessive disorder of sex development caused by a mutation of the 5alpha-reductase type 2 gene. As a result, there is an abnormality in conversion of testosterone (T) to dihydrotestosterone (DHT) and children with 5alpha-reductase deficiency are born with ambiguous genitalia. Here, we report identical twins who presented with ambiguous genitalia with a 46,XY karyotype and were diagnosed as 5alpha-reductase deficiency.
Child
;
Dihydrotestosterone
;
Disorders of Sex Development
;
Humans
;
Infant, Newborn
;
Karyotype
;
Parents
;
Puberty
;
Sexual Development
;
Testosterone
;
Twins, Monozygotic
6.Krukenberg Tumor Presenting with Brain Metastasis: A Case Report.
Taek Hyun KWON ; Tai Hyoung CHO ; Dong Jun LIM ; Jung Yul PARK ; Youn Kwan PARK ; Yong Gu CHUNG ; Hung Seob CHUNG ; Jung Keun SUH
Journal of Korean Neurosurgical Society 2000;29(9):1267-1270
No abstract available.
Brain*
;
Krukenberg Tumor*
;
Neoplasm Metastasis*
7.Hiatal Hernia in Neonate.
Yong Taek LIM ; Sung Hyuk CHUNG ; Min Yong KIM ; Byung Yul KIM ; Chung Ho LEE
The Korean Journal of Thoracic and Cardiovascular Surgery 2001;34(2):184-188
The incidence of Congenital diaphragmatic hernia is 1 in 2000-5000 live births and hiatal hernia is even rarer especially in neonates. We experienced a case of congenital hiatal hernia (mixed type) in a week old female. Upon confirmation of the diagnosis, the surgery was done. Through the right thoracotomy, Belsey-Mark IV fundoplication was performed after the reduction of herniated viscera. The patient was fed 3 days after operation. there has been no complaint for 6 months after discharge. Therefore, we present this case with overall review of the literature.
Diagnosis
;
Female
;
Fundoplication
;
Hernia, Diaphragmatic
;
Hernia, Hiatal*
;
Humans
;
Incidence
;
Infant, Newborn*
;
Live Birth
;
Thoracotomy
;
Viscera
8.Functional Cardiomyocytes Formation Derived from Parthenogenetic Mouse Embryonic Stem Cells.
Hyun Ah SHIN ; Eun Young KIM ; Young Jae LEE ; Keum Sil LEE ; Eun Mi PARK ; Hoon Taek LEE ; Kil Saeng CHUNG ; Sepill PARK ; Jin Ho LIM
Korean Journal of Fertility and Sterility 2002;29(2):139-147
OBJECTIVE: This study was to establish a reproducible differentiation system from the parthenogenetic mouse embryonic stem (P-mES02) cells into functional cardiomyocytes like as in vitro fertilization mouse embryonic stem (mES01) cells. MATERIALS AND METHODS: To induce differentiation, P-mES02 cells were dissociated and aggregated in suspension culture environment for embryoid (EB) formation. For differentiation into cardiomyocytes, day 4 EBs were treated with 0.75% dimethyl sulfoxide (DMSO) for another 4 days (4-/4+) and then were plated onto gelatin-coated dish. Cultured cells were observed daily using an inverted light microscope to determine the day of contraction onset and total duration of continuous contractile activity for each contracting focus. This frequency was compared with the results of DMSO not treated P-mES02 group (4-/4-) and mES01 groups (4-/4+ or 4-/4-). For confirm the generation of cardiomyocytes, beating cell masses were treated with trypsin-EDTA, dispersed cells were plated onto glass coverslips and incubated for 48 h. Attached cells were fixed using 4% paraformaldehyde and incubated with specific antibodies (Abs) to defect cardiomyocytes (anti-sarcomeric alpha-actinin Ab, 1: 100; anti-cardiac troponin I Ab, 1: 2000) for 1 h. And the cells were finally treated with FITC or TRITC labelled 2nd Abs, respectively, then they were examined under fluorescence microscopy. RESULTS: Rhythmically contracting areas in mES01 or P-mES02 cells were firstly appeared at 9 or 10 days after EBs plating, respectively. The highest cumulative frequency of beating EBs was not different in both treatment groups (mES01 and P-mES02, 4-/4+) with the results of 61.3% at 13 days and 69.8% at 15 days, respectively. Also the contracting duration of individual beating EBs was different from minimal 7 days to maximal 53 days. However, DMSO not treated groups (mES01 and mES02,4-/4-) also had contracting characteristics although their frequency was a few compared to those of DMSO treated groups (6.0% and 4.0%). Cells recovered from the spontaneously contracting areas within EBs in both treated groups were stained positively with muscle specific anti-sarcomeric alpha-actinin Ab and cardiac specific anti-cardiac troponin I Ab. CONCLUSION: This study demonstrated that the P-mES02 cell-derived cardiomyocytes displayed similarly structural properties to mES01 cell-derived cardiomyocytes and that the DMSO treatment enhanced the cardiomyocytes differentiation in vitro.
Actinin
;
Animals
;
Antibodies
;
Cells, Cultured
;
Dimethyl Sulfoxide
;
Embryonic Stem Cells*
;
Fertilization in Vitro
;
Fluorescein-5-isothiocyanate
;
Glass
;
Mice*
;
Microscopy, Fluorescence
;
Myocytes, Cardiac*
;
Troponin I
9.Parthenogenetic Mouse Embryonic Stem Cells have Similar Characteristics to In Vitro Fertilization mES Cells.
Se Pill PARK ; Eun Young KIM ; Keum Sil LEE ; Young Jae LEE ; Hyun Ah SHIN ; Hyun Jung MIN ; Hoon Taek LEE ; Kil Saeng CHUNG ; Jin Ho LIM
Korean Journal of Fertility and Sterility 2002;29(2):129-138
OBJECTIVE: This study was to compare the characteristics between parthenogenetic mES (P-mES) cells and in vitro fertilization mES cells. MATERIAL AND METHODS: Mouse oocytes were recovered from superovulated 4 wks hybrid F1 (C57BL/6xCBA/N) female mice. For parthenogenetic activation, oocytes were treated with 7% ethanol for 5 min and 5microgram/ml cytochalasin-B for 4 h. For IVF, oocytes were inseminated with epididymal perm of hybrid F1 male mice(1x106/ml). IVF and parthenogenetic embryos were cultured in M16 medium for 4 days. Cell number count of blastocysts in those two groups was taken by differential labelling using propidium iodide (red) and bisbenzimide (blue). To establish ES cells, blastocysts in IVF and parthenogenetic groups were treated by immunosurgery and recovered inner cell mass (ICM) cells were cultured in LIF added ES culture medium. To identity ES cells, the surface markers alkaline phosphatase, SSEA-1, 3, 4 and Oct4 staining were examined in replated ICM colonies. Chromosome numbers in P-mES and mES were checked. Also, in vitro differentiation potential of P-mES and mES was examined. RESULTS: Although the cleavage rate (> or =2-cell) was not different between IVF (76.3%) and parthenogenetic group (67.0%), in vitro development rate was significantly low in parthenogenetic group (24.0%) than IVF group (68.4%) (p<0.05). Cell number count of ICM and total cell in parthenogenetic blastocysts (9.6+/-3.1, 35.1+/-5.2) were significantly lower than those of IVF blastocysts (19.5+/-4.7, 63.2+/-13.0) (p<0.05). Through the serial treatment procedure such as immunosurgery, plating of ICM and colony formation, two ICM colonies in IVF group (mES, 10.0%) and three ICM colonies (P-mES, 42.9%) in parthenogenetic group were able to culture for extended duration (25 and 20 passages, respectively). Using surface markers, alkaline phosphatase, SSEA-1 and Oct4 in P-mES and mES colony were positively stained. The number of chromosome was normal in ES colony from two groups. Also, in vitro neural and cardiac differentiation derived from mES or P-mES cells was confirmed. CONCLUSION: This study suggested that P-mES cells can be successfully established and that those cell lines have similar characteristics to mES cells.
Alkaline Phosphatase
;
Animals
;
Antigens, CD15
;
Bisbenzimidazole
;
Blastocyst
;
Cell Count
;
Cell Line
;
Embryonic Stem Cells*
;
Embryonic Structures
;
Ethanol
;
Female
;
Fertilization in Vitro*
;
Humans
;
Male
;
Mice*
;
Oocytes
;
Propidium
10.Four Cases of Papillary Eccrine Adenoma.
Ji Hyun LIM ; Shin Taek OH ; Hyung Ok KIM ; Chung Won KIM ; Young Min PARK ; Key Young SONG
Korean Journal of Dermatology 2003;41(6):761-765
Papillary eccrine adenoma is a rare cutaneous tumor which occurs most commonly on the distal portion of extremities as a small solitary nodule. Histopathologically, the tumor consists of multiple dilated or branching tubular structures that are lined by two or more layers of epithelial cells and shows papillary projection into the lumina. Positive staining with S-100 protein, CEA, EMA and keratin in papillary eccrine adenoma is consistent with differentiation toward the secretory epithelium of eccrine sweat gland. Herein we report four cases of papillary eccirine adenoma and review the literature.
Adenoma*
;
Epithelial Cells
;
Epithelium
;
Extremities
;
S100 Proteins
;
Sweat Glands