1.Clinical Observation On The CPT-11 Plus 5-FU For treatment Advanced Rectal Cancer
Weijun TANG ; Hongyuan ZOU ; Chunfang PENG
Chinese Journal of Primary Medicine and Pharmacy 2011;18(2):161-162
Objective To evaluate the efficacy and adverse events of irinotecan (CPT-11) combined with 5-FU in treatment of patients with advanced rectal cancer. Methods 86 patients were randomly divided into FOLFIRI treatment group and IFL treatment group. The efficacy and adverse events were observed. Results There was no statistically significant difference between FOLFIRI treatment group and IFL treatment group in CR、PR、SD and RR(all P > 0. 05). There was also no statistically significant difference between FOLFIRI treatment group and IFL treatment group in adverse events(all P > 0.05). Conclusion Irinotecan in combination with 5-FU was effective and had some tolerable adverse events in treating patients with advanced rectal cancer in both FOLFIRI treatment group and IFL treatment group.
2.Trichostatin A inhibits serum-induced p27kip1 protein degradation in vascular smooth muscle cells
Chen ZOU ; Chunfang WU ; Zhihong XU ; Guoping LU
Chinese Journal of Pathophysiology 2000;0(07):-
AIM:To investigate the effect of trichostatin A(TSA)on p27kip1 gene expression in vascular smooth muscle cells.METHODS:Reverse transcription-polymerase chain reaction(RT-PCR)was used to measure the level of p27kip1 mRNA.The protein levels of p27kip1 and S-phase kinase-associated protein-2(skp2)were determined by Western blotting.20S proteasome activity was quantified by using a fluorogenic proteasome-specific substrate.RESULTS:TSA did not affect mRNA level of p27kip1 in VSMCs,but attenuated serum-induced downregulation of p27kip1 through stabilizing p27kip1 turnover.In addition,TSA decreased the expression of skp2,an F-box protein that targets p27kip1 for degradation,but had no effect on proteasome activity.CONCLUSION:TSA regulates p27kip1 expression at the post-translational level in VSMCs.
3.Effect of trichostatin A on proliferation and apoptosis of vascular smooth muscle cells
Chen ZOU ; Chunfang WU ; Zhihong XU ; Guoping LU
Chinese Pharmacological Bulletin 2003;0(12):-
Aim To study the effect of TSA on vascular smooth muscle cells(VSMC)proliferation and apoptosis in vitro.Methods VSMC proliferation was analyzed by MTT assay and BrdU incorporation assay.Cell cycle phase distributions were determined by flow cytometer.The expressions of cyclin D1 and cyclin A were assessed by western blot.Cell apoptosis was quantified by detecting cytoplasmic histone-associated DNA-fragments and the level of cleaved caspase-3.Results TSA at a low concentration was adequate to inhibit serum-induced VSMC proliferation without significant cytotoxity.High concentration of TSA activated caspase-3 and induced VSMC apoptosis.TSA treatment reduced expressions of cyclin D1 and cyclin A,and blocked VSMC entry into S phase.Conclusions TSA inhibits serum-induced VSMC proliferation and G1→S phase progression of cell cycle.Histone deacetylase(HADC)inhibitors may constitute a novel therapy for vascular proliferative diseases.
4. Investigation and analysis on knowledge and attitude of Cancer pain management among 232 nursing staff in oncology department
Dan LI ; Juanhua SUN ; Chunfang ZOU ; Xin WANG ; Danhuan WANG ; Lixia YUE ; Ping JIANG ; Yu MA ; Yuhan LU
Chinese Journal of Practical Nursing 2019;35(21):1651-1656
Objective:
To investigate the current status of knowledge and attitudes of oncology nursing staffs on pain management in cancer patients.
Methods:
A total of 232 oncology nursing staffs from 7 Grade III-A Hospitals in 4 provinces and municipalities were surveyed using General Information Questionnaire and knowledge and attitudes survey regarding pain (KASRP) (2014) questionnaire (Chinese version).
Results:
The results of this survey showed that the average score of knowledge and attitude about pain management of 232 oncology nursing staffs was (21.56±4.01) points, and the average correct rate was 52.59%. There were no significant differences in the scores of different education background, years of nursing work, years of cancer clinical nursing care, and the nature of the hospital they worked in(