Hepatocellular carcinoma(HCC) is the fifth most common cancer and the third cause of cancer-related death worldwide;meanwhile,it is also one of the fastest growing malignancies.The causes of HCC are multiple;HBV is the most important cause in China,with about 25%-30% of HBV infection patients finally develop hepatic cirrhosis and liver cancer.At present,large scale epidemiological studies revealed that the metabolic syndrome(MS) was closely related to liver cancer,and it even served as an independent risk factor for the occurrence and progression of liver cancer.Non-alcoholic fatty liver disease is a metabolic syndrome clinically manifested in the liver;recently it has been indicated to be closely related with HCC development and progression.This paper reviews the recent researches on metabolic syndrome and liver cancer,so as to provide literature for preventive and therapeutic studies on non-virus-related liver cancer.

Objective To approach the feasibility of identifying the specific biomarker of ovarian cancer by SELDI TOF mass spectrometry. Methods The relative contents of serum protein of both 24 the patients with ovarian cancer and 56 cases of healthy people were tested by IMAC3 chip and proteinchip reader (CipherGen Inc., VS). Results On the M/Z values ranged from 4000Da to 10000Da, there were six kinds of protein contents in the serum of the were obviously different between the two groups. Among them the serum protein of M/Z value 4472Da may be regarded as a specific biomarker of ovarian cancer. In the learning mode, all the 24 patients and 56 control people were diagnosed and distinguished out correctly, while in the test mode, 23 patients were correctly diagnosed and 56 control people were distinguished out, the total accuracy was 98 75%(79/80), and the sensitivity and specificity were 95 8%(23/24) and 100%(56/56), respectively. Conclusion Ovarian cancer can be quickly and correctly diagnosed by this method with high sensitivity and specificity. That will be widely used in clinical application

Objective To approach the significance of serum protein fingerprinting on the identification of different courses of acute myocardial infarction. Methods For 153 patients of acute myocardial infarction, including 45 cases of just admitted patients, 12 cases of 3h admitted patients, 22 cases of 6h admitted patients, 24 cases of 9h admitted patients, 24 cases of 12h admitted patients, 16 cases of 24h admitted patients and 10 cases of 48h admitted patients. The relative contents of serum proteins were detected by IMAC3 chip and proteinchip reader (CipherGen Inc., VS). Results On the M/Z values ranged from 4KDa to 12KDa, ten protein contents were obviously different between the different courses of acute myocardial infarction. All the patients in different courses were diagnosed correctly, the accuracy was 100%(153/153). Both sensitivity and specificity were also 100% in learning mode. Conclusion Different courses of acute myocardial infarction patients can be quickly and correctly diagnosed by this method with high sensitivity and specificity. That will be widely used in clinical application

Objective To establish a serum protein fingerprinting technique with a pattern matching algorithm to distinguish Dukes A stage from colorectal cancer of Dukes B,C,D stage. Methods Serum samples were collected from both reserved group and test group, each of the groups comprised 10 patients of colorectal cancer in Dukes A stage and 68 patients in Dukes B,C,D stage. The sera of the reserved group was combined with the surface of the IMAC3 proteinchip. Then the data of SELDI TOF MS was read and analyzed by BioMarker Wizard software and BioMarker Pattern software to get a classificatory pedigree tree, which is a standard configuration that can distinguish the sera of colorectal cancer patients of Dukes A from colorectal cancer patients of Dukes B,C,D, and the standard was confirmed by double blind test in the test group. Results At the M/Z values of 8 320 Da, 8 604Da, 8 867Da and 15 872Da, the protein contents in reserved group are obviously different between the two classes of patients. The accuracy of classification was 97 4%(76/78), corresponding sensitivity was 100%(10/10) and corresponding specificity was 97 1%(66/68). By double blind examination in test group, the corresponding accuracy was 100%(10/10), the corresponding sensitivity was 94 1%(64/68) and the corresponding specificity was 94 1%(64/68). Conclusion Colorectal cancer of Dukes A can be quickly and exactly diagnosed by this method with high sensitivity and specificity. That will be widely used in clinical application

Objective To search for the biomarker used to determine gastric cancer by the application of protein mass spectrometry analysis. Methods The relative contents of serum proteins were detected of 38 patients with gastric cancer and 82 healthy people by IMAC3 (CipherGen Inc.) chip and proteinchip. Results At the M/Z values range from 1 723Da to 14 048Da, 18 kind of protein contents are obviously different between the two groups. In the learning mode, all the 120 testers were correctly distinguished, both the sensitivity and specificity reached to 100%. While in the test mode, 31 patients and 81 control people were correctly distinguished, the accuracy were 81 6%(31/38) and 98 8%(81/82), respectively. Conclusion Gastric cancer can be quickly and exactly diagnosed by this method with high sensitivity and specificity. That will be widely used in clinical application

Objective To investigate the clinical manifestations,pathological features,treatment and prognosis of nongesta-tional choriocarcinomas.Methods The clinicopathological data in 4 cases of nongestational choriocarcinoma were retrospectively analyzed and the relevant literatures were reviewed.Results All 4 patients had no confirmed diagnosis at first visit to hospital.All patients received the operative therapy and postoperative bleomycin,etoposide and cis-platinum (BEP)regimen chemotherapy.The followed up lasted for 3-32 months.One case died at postoperative 26 months due to pulmonary metastasis;one case was loss of follow up;two cases had relatively favorable condition.Conclusion Nongestational choriocarcinoma is a rare and highly invasive trophoblastic neoplasm,and is prone for early distant metastasis.The treatment is recommended to adopt operation combined with postoperative combined chemotherapy of multiple drugs.

The expression cDNA library of A. tabescens was constructed by SMART technique, which useλTriplEx2 as a vector. The titer and the percentage of the constructed library were about 1.0 × 106pfu/mland 98.3% respectively, and the titer and the capacity of the amplified library were about 3.1 × 108pfu/mland 4.2 × 1010. The library was used to provide expressed sequence tags (ESTs). 147 Expressed SequenceTaqs (ESTs) were gained from 176 clones, which were selected randomly and sequenced at the 5'end. Thesequences were submitted to the EMBL database. Blasting the sequences in the GenBank, 43 of them werefound that they have significant similarity with data in GenBank. EST AJ620046 was has significantsimilarity with the arabinosidase of Bacteroides thetaiotaomicron. Using SMART-RACE a full-length cDNA ofAJ620046 was successfully obtained. In order to initially characterize the biochemical properties ofAJ620046, the ORF of AJ620046 named AF was cloned and expressed in Pichia Pastoris yeast.Recombinant pHIL-S1-AF constructed by inserting AF into pHIL-S1 was transformed into Pichia PastorisGS115. Preliminary experiments indicated that AJ620046 was expressed as a 32 kDa protein in recombinantyeast.

Objective To investigate the influencing factors of chronic brucellosis.Methods Nested case control method was used to study newly diagnosed patients (n =600) with brucellosis in a cohort study in 2012.Data of general characterstics,clinical presentation,treatment and prognosis of those patients were collected.These patients were followed up for one year,and the chronic patients as the case group (n =248) and the healed patients as a control group (n =260).By means of Logistic multivariate analysis,factors turned brucellosis into chronic were screened.Result The chronic brucellosis-related factors were:gender,veterinary or epidemic prevention staff,muscle and joint pain,fatigue symptoms,and substandard treatment (x2 =5.163,16.445,14.977,17.154,8.813,all P ＜ 0.05).Conclusion Gender (female),veterinary or epidemic prevention staff,muscle and joint pain,fatigue symptoms,and substandard treatment are probably the chronic brucellosis-related factors

Four compounds were isolated from the fruit of Panax quinquefolius L. , They were iden-tified as 20 (R)-ginsenoside-Rg3( Ⅲ ), ginsenoside-Ra1( Ⅱ ), pseudo ginsenoside-RTs (iⅣ) and a new com-pound named quinquetriose ( Ⅰ ). Its structure was established as β3-D-xylopyranosyl-(1→6)-α-D-glucopy-ranosyl (1→6)-β-D-glucopyranoside, on the basis of chemical and spectroscopic evidences.

Objective To evaluate the method and outcome of surgical treatment for refractory slow-transit constipation (STC). Methods Clinicopathologic data of 24 patients with severe STC which underwent (colectomy) in recent years were retrospectively analyzed. Results 18 of the patients underwent pancolectomy,and 6 underwent subtotal colectomy. All of the 14 STC patients complicated with outlet obstructive (constipation)(OOC) were corrected preoperatively by related surgery. The curative rate of surgical (treatment) was 95.8%,with an average of(3?1.9) times of bowel motion per day. The incidence of (abdominal) distention decreased from 75% to 12.5%. One patient who underwent ileorectal anastomosis (developed) recurrent constipation. Conclusions Colectomy produces satisfactory results in the majority of (patients) with refractory slow-transit constipation.Patients complicated with OOC should undergo energetic (preoperative) correction therapy,in order to ensure a satisfactory surgical outcome.