Objective To determine if propofol can affect uptake of dopamine ( DA) by dopamine transporter (DAT) . Methods Chinese hamster ovary (CHO) cells in which DAT was highly expressed were incubated with H-dopamine in the presence or absence of propofol. Uptake of H-DA was measured. The kinetic parameters Vmax and Km were calculated. Results Propofol significantly inhibited uptake of DA by DAT in CHO cells as compared with the control ( P

Background:Gankyrin is an ankyrin repeat oncoprotein overexpressed and involved in the tumorigenesis and progression of various cancers. Aims:To investigate the effect and underlying mechanism of down-regulation of gankyrin expression on proliferation of gastric cancer cells. Methods:Lentivirus vector carrying gankyrin-targeted siRNA was transfected into human gastric cancer cell line MKN28. Cell proliferation,cell cycle distribution and β-catenin/ cyclin D1 signaling pathway was analyzed by MTT assay,flow cytometry and Western blotting,respectively,in gankyrin-silenced MKN28 cells and control cells. Results:The transfection efficiency of lentivirus vector was more than 90% ,and the protein expression of gankyrin in gankyrin siRNA transfected MKN28 cells was significantly repressed( P ﹤ 0. 01). Compared with cells transfected with control lentivirus and cells without transfection,MKN28 cells transfected with gankyrin siRNA showed markedly repressed cell growth after 3-day-culture;the proportion of cells in cell cycle G1 phase was significantly increased,and that in S phase was significantly decreased;down-regulated expression of β-catenin and cyclin D1 was observed(P all ﹤ 0. 01). Conclusions:Down-regulation of gankyrin expression in gastric cancer cells may induce cell cycle G1 phase arrest and inhibit cell proliferation by suppressing β-catenin/ cyclin D1 signaling pathway. Gankyrin might be a promising novel target for targeted therapy of gastric cancer.

Objective: To evaluate the outcomes of splenectomy in the treatment of relapsed/refractory autoimmune hemolytic anemia (AIHA). Methods: Retrospective analysis was performed in 30 cases with relapsed/refractory AIHA who were treated with splenectomy in our hospital. The pre- and post-operative blood routine indexes and responses were followed up. Results: Among the 30 relapsed/refractory AIHA patients, 20 were pure AIHA (including 13 patients with warm antibody AIHA, 2 with warm-cold double antibody AIHA and 5 with Coombs negative AIHA) and 10 were Evans syndrome. The short-term response was evaluated 10-14 days after operation, and the overall response rate (ORR) of short-term response was 90% [12 cases in complete response (CR), 6 cases in partial response (PR)] in 20 therapeutic evaluable cases. Among 13 patients with long-term follow-up data, except 3 patients with Evans syndrome died (2 cases were refractory to splenectomy, 1 case relapsed after surgery), the ORR of 10 patients with relapsed/refractory pure AIHA at 6 months and 12 months were 90% (9/10) and 70% (7/10), respectively, with a median follow-up of 14 (4-156) months. At the end of follow-up, 3 cases had maintained CR for more than 3 years. Conclusion The short-term response of splenectomy as a second-line treatment for relapsed/refractory AIHA is satisfactory, and long-term outcome of splenectomy is up to 70% at 1 year. Approximately one-third of patients could maintain sustained remission.

Objective To investigate the risk factors of surgical site infection (SSI) related complications after radical gastrectomy for gastric cancer in elderly patients.Methods The clinical data of 410 elderly patients with gastric cancer who underwent radical gastrectomy was retrospectively collected from 2009 to 2016.Univariate and multivariate analysis were performed to investigate the risk factors of SSI related complications,and the impact of SSI on short-term prognosis.Results SSI developed in 50 out of 410 elderly patients who underwent radical gastrectomy for gastric cancer,including 19 incisional infections and 31 organ lacuna infections.The corresponding incidence was 12.2％,4.6％ and 7.6％,respectively.By univariate analysis,age ＞ 75 (x2 =5.315,P =0.021),preoperative anemia (x2 =3.983,P =0.046),NRS 2002 ≥ 3 (x2 =4.785,P =0.029),diabetes (x2 =5.895,P =0.015),preoperative obstruction (x2 =5.250,P =0.022),undifferentiated carcinoma (x2 =4.448,P =0.035),cardiac carcinoma (x2 =5.265,P =0.022) and combined organs resection (x2 =4.165,P =0.041) were associated with SSI.Multivariate analysis showed that advanced age (OR =2.422,P =0.016),diabetes (OR =2.524,P =0.026),preoperative obstruction (OR =2.098,P =0.047) and high NRS 2002 score (OR =1.969,P =0.043) were independent risk factors for SSI.Conclusion The independent risk factors of SSI for elderly gastric cancer patients are advanced age,diabetes,preoperative obstruction and high NRS 2002 score.