Objective To explore the application of serum dust mite specific IgE detection in the diagnosis and treatment of child asthma .Methods 52 children cases of asthma in this hospital from February 2008 to April 2010 were selected as the observation group ,11 cases were <3 years old ,18 cases were 3- < 7 years old and 23 cases were 7- <12 years old ;44 healthy children were taken as the control group ,8 cases were <3 years old ,16 cases were 3- < 7 years old and 20 cases were 7- <12 years old .The two groups were detected the serum dust mite specific Ig E .Results The serum dust mite specific Ig E was (152 .78 ± 48 .95) U/mL in the observation group and (25 .62 ± 6 .49) U/mL in the control group ,the serum dust mite specific Ig E concentration in the observation group was significantly higher than that in the control group ,the difference showed the statistical significance (P<0 .05) .Conclusion The positive rate of serum dust mite specific IgE in child asthma is very high ,especially the children patients of 7- <12 years old have the highest positive rate .Therefore ,the dust mite allergen is the important factor inducing child asthma .The detection of serum dust mite specific IgE is conducive to find the dust mite allergen ,which is an important measure for preventing and treating child asthma induced by the dust mite allergen and is worthy of being promoted in clinic .

Objectives To analyze the relationship between the multi-organ hypoxia damage and the death of acute high altitude sickness severe case. Methods 3220 cases from 1956. 06 to 2005.06 in the hospital were reviewed by questionnaire and the cases in MODS or death were screened out. The cause of death and feature were analyzed by the data as clinical symptom, physical signs and auxiliary examination. Results 281 cases complicated by MODS (include 35 dead cases) were found out by review. The incidence with MODS is 8.73% , and the death rate was 1.09%. The masculine incidence rate of the clinical symptom, physical signs and auxiliary examination in death were 2. 86% ~65. 71% , 2. 86% ~ 100% and 14. 29% ~ 100% , respectively. The MODS incidence in dead cases was 100%. Compared with the curing group, the MODS number, cardiac functional disturbance, brain disorder and renal functional disturbance in the death group had a significantly high incidence rate in all the 281 cases with MODS( P <0.05). With dependability analysis, there were a significantly direct correlation relationship between the death in the severe cases with acute high altitude sickness and the number of MODS ( r = 0. 3473, P < 0.001). Conclusion Hypoxia to the damage of multi-organ function play an important role in death of the severe cases with acute high altitude sickness in high attitude area, and the functional disturbance of important organs as heart, brain and kidney is the most important cause to death.

OBJECTIVETo study the effect of osteogenic inducer (dexamethasone, beta-sodium glycerophosphate and Vitamin C) carried by gelatin sponge on healing and remodeling of tooth extraction sockets.

METHODSFifty rabbits were selected. After extracting the first premolars of bilateral maxillary, the right side tooth extraction sockets were filled with gelatin sponge containing osteogenic inducer as experimental side, tooth extraction sockets on left side were filled with gelatin sponge as control. Every ten rabbits were executed at the end of 1, 2, 4, 8, 12 weeks after tooth extraction. Bone density was measured through digital X-ray images. The specimens were examined by histology. The absorption height of alveolar bone at 12 weeks was measured.

RESULTSX-ray measurement showed that the bone density of experimental side was higher than that of control side at 2, 4, 8, 12 weeks, the difference had statistical significance (P<0.01). The histology examination showed that new bone formation in tooth extraction sockets of experimental side was earlier than that in control side. The absorptional height of alveolar bone had significant difference between experimental side and control side (P<0.01), of which experimental side was less.

CONCLUSIONFilling the osteogenic inducer in tooth extraction sockets can promote the healing and new bone formation and prevent from alveolar bone absorption.

Animals ; Bicuspid ; Maxilla ; Rabbits ; Tooth Extraction ; Tooth Socket ; Wound Healing

OBJECTIVEThrough the comparison of Xiaoyu ointment and xiaoyu plaster by in vitro transdermal demonstrate, to demonstrate the scientificity and feasibility of reformed formulation.

METHODThe improved Franz diffusion cells and in vitro rabbit skin were used in vitro penetration experiment with emodin as an indicator of penetration rate quantitated by HPLC.

RESULTThe cumulative penetration rate of emodin in Xiaoyu ointment fit the model of Weibull distribution, while the cumulative penetration rate of emodin in Xiaoyu plaster fit the model of Density equation. Take emodin as an index,the transdermal rate in Xiaoyu plaster was 1.93 times as Xiaoyu ointment, and the total penetrated amount was 2.84 times as Xiaoyu ointment. The results showed that the emodin of xiaoyu plaster reserved in the skin were 3.95 times more than the ointment.

CONCLUSIONThe penetration rate, total penetrated amount and the reserves in the skin of Xiaoyu plaster were better than the ointment, and the transdermal dosage form was better than the original form.

Administration, Cutaneous ; Animals ; Chromatography, High Pressure Liquid ; Ointments ; pharmacokinetics ; Rabbits ; Skin ; metabolism ; Skin Absorption

Under low temperature conditions, the hapten carboxyl-norketamine was synthesized by reacting norketamine and succinaldehyde acid.Identification result using electrospray ionization mass showed the hap ten was successfully synthesized.The artificial antigen confirmed by infrared spectroscopy was developed by conjugating hapten to carrier proteins with carbodiimide(EDC) method.Matrix-assisted laser desorption ioni zation time of flight mass spectrometry(MALDI-TOF-MS) showed that the ratio of hapten to BSA was 11:1.The antibody with high titer(5.12 × 10~4) was produced after immuning to rabbits.

Objective: To understand the viral etiology of a clustered case of human infection outbreak in the middle school of Huai’an city. Methods: Nasopharyngeal swab samples from patients were collected and rapidly detected by Real-time RT-PCR and the target virus isolated in cells. Furthermore, HA1 segments of target virus were amplified by RT-PCR and sequenced. The genetic and phylogenetic analysis based on HA1 genes was computed. Results: Influenza A(H1N1)pdm09 viral nucleic acid in 11 nasopharyngeal swab samples from patients in the outbreak were positive. Compared to the vaccine strains A/California/07/2009, the Huai’an isolates, nucleotide identity was 97.7%-98.1%, and amino acid identity was 96.6%-97.4%. Phylogenetic analysis of HA1 segment sequences indicated that the Huai’an strains from the outbreak were related closely to the viruses isolated in the year of 2014. Sequence analysis indicated that the Huai’an isolates had no amino acid substitution in the receptor binding sites and glycosylation sites, while in the Ca1 of antigenic determinant of HA1 the Huai’an isolates had an amino acid substitution of S for T at 220. Conclusions The pathogen of the clustered case of human infection was Influenza A(H1N1)pdm09 virus. Though the Huai’an isolates had one animo acid substitution in the Ca1 of antigenic determinant, the antigenicity characteristic remained unchanged.