Adult ; Aged ; Apoptosis ; Female ; Humans ; Male ; Middle Aged ; Nitric Oxide ; metabolism ; Nitric Oxide Synthase Type II ; metabolism ; Pressure Ulcer ; metabolism ; pathology ; Skin ; metabolism ; pathology ; Tumor Suppressor Protein p53 ; metabolism

China ; epidemiology ; Disease Outbreaks ; Female ; Humans ; Incidence ; Male ; Measles ; epidemiology ; Meteorological Concepts ; Seasons

[Summary] According to urinary albumin excretion rates ( UAER) , 256 patients with type 2 diabete mellitus (T2DM) were divided into normal albuminuria (NA), microalbuminuria (MA), and clinical nephropathy (CN) groups while 108 healthy subjects as control group. The analysis of variance of single factor was applied to examine lipoprotein(a), triglyceride, total cholesterol(TC), low-density lipoprotein cholesterol(LDL-C), cystatin C, and homocysteine. The correlations of lipoprotein ( a ) with urinary albumin excretion rate ( UAER ) and glomerular filtration rate ( eGFR ) were analyzed by Pearson correlation analysis. The sensitivities of lipoprotein ( a ) were evaluated in diagnosis of diabetic nephropathy ( DN) by receiver operating characteristic curve. The results showed that lipoprotein ( a) levels in NA, MA, and CN groups were gradually increased, with a significant increase in CN group(P<0. 05). Pearson correlation analysis revealed that lipoprotein (a) was positively correlated with systolic pressure, TC, cystatin C, and UAER(all P<0. 05) and negatively correlated with fasting blood glucose and eGFR (P<0. 05). The area under the ROC curve of lipoprotein(a) was 0. 639, with the sensitivity 66. 4% and specificity 55. 9% in the optimal cutoff value of 8. 41 mg/dl. These results suggest that lipoprotein(a) may serve as an index for monitoring DN based on its better correlation with UAER and eGFR.

Aged ; Aged, 80 and over ; Delivery of Health Care ; Family Characteristics ; Female ; Home Care Services ; Humans ; Male ; Middle Aged ; Rural Population ; Surveys and Questionnaires

Traditional Chinese medicine (TCM) reference standards plays an important role in the quality control of Chinese herbal pieces. This paper overviewed the development of TCM reference standards. By analyzing the 2010 edition of Chinese pharmacopoeia, the application of TCM reference standards in the quality control of Chinese herbal pieces was summarized, and the problems exiting in the system were put forward. In the process of improving the quality control level of Chinese herbal pieces, various kinds of advanced methods and technology should be used to research the characteristic reference standards of Chinese herbal pieces, more and more reasonable reference standards should be introduced in the quality control system of Chinese herbal pieces. This article discussed the solutions in the aspect of TCM reference standards, and future development of quality control on Chinese herbal pieces is prospected.
Drugs, Chinese Herbal ; standards ; Humans ; Medicine, Chinese Traditional ; standards ; Quality Control ; Reference Standards

Classical Swine Fever Virus (CSFV) E2 protein eukaryotic expression plasmid pVAXE2 was constructed. The plasmid pVAXE2 was transformed into Salmonella choleraesuis C500 (S. C500) attenuated vaccine strain by electroporation to generate Salmonella choleraesuis engineering strain S. C500/pVAXE2. The characterization of S. C500/pVAXE2 in morphology, growth, biochemistry and serology indicated that it retained the same properties as its original strain S. C500 with exception of kanamycin resistance originated from the plasmid pVAXE2. The plasmid stable in the bacteria after 15 passages. Kunming mice and rabbits were vaccinated three times at two weeks interval with S. C500/pVAXE2 in oral and intramuscular routes at the dosage of 1 x 10(8) CFU for mice and 2 x 10(9) CFU for rabbits each time. The specific antibody response against CSFV and Salmonella choleraesuis was detected by ELISA. Two weeks after the third boost the immunized rabbits were challenged with 20 ID50 of hog cholera lapinized virus (HCLV), followed by a virulent strain of Salmonella choleraesuis two week later than HCLV challenge. The results showed that all immunized mice and rabbits produced significant antibodies against CSFV and Salmonella choleraesuis, and the immunized rabbits demonstrated the effective protection against the challenge of HCLV and virulent Salmonella choleraesuis. These results indicated the potential of developing multiplex swine DNA vaccine by using this bacteria as the vector.
Animals ; Classical Swine Fever ; immunology ; prevention & control ; virology ; Classical swine fever virus ; genetics ; immunology ; Mice ; Rabbits ; Salmonella arizonae ; genetics ; Swine ; Vaccines, DNA ; immunology ; Viral Envelope Proteins ; biosynthesis ; genetics ; immunology ; Viral Vaccines ; immunology

OBJECTIVETo study the preparation technique of the liposome of Bupleurum chinense volatile oil.

METHODThe composition, entrapment ratio of volatile oil, availability of freeze-drying and the changes of finger prints were studied.

RESULTEntrapment ratio reached 81.9%. After freeze-dried, the remaining ratio of the oil entrapped in the liposome reached 88.6%.

CONCLUSIONIt is practicable to make B. chinense injection into freeze-dried liposome powder.

Bupleurum ; chemistry ; Freeze Drying ; Injections ; Liposomes ; Oils, Volatile ; administration & dosage ; isolation & purification ; Plants, Medicinal ; chemistry ; Powders ; Technology, Pharmaceutical ; methods

OBJECTIVETo investigate the effect of telomerase reverse transcriptase (TERT) to the proliferation of 5-HT induced pulmonary artery smooth muscle cells (PASMCs).

METHODSThe PASMCs proliferation experiment was performed to detect the effort on PASMCs of 5-HT or ASODN TERT (antisense oligoribonucleotides TERT designed according to the rat TERT mRNA sequence of gene bank). The immunohistochemistry staining experiment and the in situ hybridization experiment were to detect the TERT protein and mRNA expression with 5-HT or ASODN TERT. FITC marked ASODN TERT experiment was done to research the distribution of ASODN TERT in PASMCs.

RESULTS5-HT promoted PASMCs proliferation in a dose-dependent manner (10(-9) - 10(-5) mol/L). 5-HT also significantly increased TERT expression at protein and mRNA levels as shown by immunohistochemistry staining and the in situ hybridization studies. This effect could be blocked by ASODN TERT in a time and dose-dependent manner.

CONCLUSIONSOur experiments show TERT is one of the key factors in the procession of 5-HT induced PASMCs proliferation. ASODN TERT might be a potential therapy agent for pulmonary hypertension.

Animals ; Cell Proliferation ; Cells, Cultured ; Muscle, Smooth, Vascular ; cytology ; Myocytes, Smooth Muscle ; cytology ; Pulmonary Artery ; cytology ; RNA Replicase ; RNA, Messenger ; genetics ; Rats ; Serotonin ; pharmacology ; Telomerase ; pharmacology

Objective To explore the prognostic factors of community -acquired pneumonia (CAP)among children. Methods A total of 783 children with CAP admitted to hospital from 2009 to 2012 were selected.Related information including baseline data was extracted from the hospital health records and analyzed by univariate and multiple logistic regression analysis.Results The logistic regression analysis showed that obnubilation (OR =5.866,95%CI =2.020 -17.033),dysphoria (OR =1.420,95%CI =1.038 -1.942),multi -infection(OR =1.376,95%CI =0.993 -1.907) and polypnea(OR =1.026,95%CI =1.004 -1.049)were the independent risk factors that influenced the prognosis of patients with community -acquired pneumonia.Conclusion More attentions should be paid to CAP children with the symptoms of obnubilation,dysphoria,multi -infection and polypnea.

Objective To explore the detection rate of depression among junior middle students in Shaoxing city,and to analyze its influencing factors.Methods Based on the cluster random sampling method,A total 3 397 students from junior middle school were recruited to be evaluated by Depression Self -Rating Scale for Children (DSRSC).Results The overall detection rate of depression was 37.74%.The average score was (1 3.24 ±5.01 ).Multiple -factors analysis showed that female (OR =1 .31 2,95%CI =1 .1 26 -1 .528),senior grade (OR =1 .284,95%CI =1 .1 70 -1 .409)and non -only -child (OR =1 .390,95%CI =1 .1 95 -1 .61 8)were the risk factors of depression,light study burden (OR =0.661 ,95%CI =0.589 -0.742),more close friends (OR =0.728,95%CI =0.666 -0.796),outgoing personality (OR=0.875,95%CI =0.81 3 -0.942)and satisfaction with body figure (OR =0.678,95%CI =0.625 -0.735)were the protective factors of depression.Conclusion The prevalence of depression among junior middle school students in Shaoxing was high.Intervention measures need to be strengthened for the psychological health especially among population with depressive symptoms.