OBJECTIVE: To investigate the relationship between the expression of vascular endothelial growth factor C (VEGF-C) and angiogenesis and lymphangiogenesis in papillary thyroid carcinoma (PTC). METHODS: Seventy-two PTC cases were divided into 3 groups according to the level of invasion: papillary microcarcinoma group (PMC group), intrathyroid carcinoma group (IPC group), and extrathyroid carcinoma group (EPC group). They were again divided into 2 groups according to lymph node metastasis: lymph node metastasis group and lymph node no-metastasis group. The expressions of VEGF-C, CD105 and vascular endothelial growth factor receptor-3 (VEGFR-3) were detected by SP method of immunohistochemical staining. The expression of VEGF-C was analyzed quantitatively by image analysis system, and the PI of VEGF-C (VEGF-C-PI), the number of MVD (microvessel density), and LVD (lymphaticvessel density) were obtained. RESULTS: The VEGF-C-PI of lymph node metastasis group (23.15 +/- 3.75) was higher than that of lymph node non-metastasis group (14.54 +/- 2.93) (P <0.01). MVD was 35.25 +/- 2.06 in the PMC group, 41.75 +/- 5.46 in the IPC group, and 52.58 +/- 4.16 in the EPC group, which showed the elevatory tendency with the increase of invasion (P < 00.5). LVD was 6.00 +/- 0.81 in the PMC group, 13.80 +/- 1.81 in the IPC group, and 19.17 +/- 2.96 in the EPC group, which again showed the elevatory tendency with the increase of invasion (P <0.05). The LVD of lymph node metastasis group (19.56 +/- 2.45) was significantly higher than that of lymph node non-metastasis group (12.48 +/- 2.84) (P < 0.05). VEGF-C was positively correlated with MVD and LVD (r = 0.743, 0.90, P <0.01). CONCLUSION The expressions of VEGF-C and LVD are related to lymph node metastasis of PTC. MVD and LVD are related to the invasion of PTC. VEGF-C may play an important role in the angiogenesis and lymphangiogenesis.
Adenocarcinoma, Papillary ; blood supply ; metabolism ; pathology ; Adolescent ; Adult ; Aged ; Child ; Female ; Humans ; Lymphangiogenesis ; Male ; Middle Aged ; Neovascularization, Pathologic ; metabolism ; Thyroid Neoplasms ; blood supply ; metabolism ; pathology ; Vascular Endothelial Growth Factor C ; biosynthesis ; genetics

Objective To investigate the methods and clinical significance of breast cancer treated with photodynamic.Methods From June to December in 2005,photodynamic therapy was used in 12 cases confirmed intramammary lymph node metastasis before operation and 15 cases confirmed chest wall recur- rences by means of lymph node imaging.Results The intramammary lymph node metastasis whose diameter between 0.5～1.0cm measured by lymph node imaging preoperatively completely disappeared when rechecked 3 months postoperatively.Chest wall recurrence regions of breast cancer whose diameter less than 1.0 cm completely remitted.Conclusion Photodynamic therapy is helpful to eliminate the intramammary lymph node metastasis and to cure the postoperative chest wall recurrence of breast cancer.

Objective To explore the diagnostic merits of the average apparent diffusion coefficient(ADCav) for leukoencephalopathy in neonates and children.Methods One hundred and fifty-six neonates and children with central nervous system signs or symptoms were classified into 6 groups according to their ages(1 d-0.05).Contrast to the normal,the ADCav of leukoencephalopathy in neonates and children decreased.With increasing age,there showed a linear downtrend in each group.Conclusions The ADCav rises in neonates and children with leukoencephalopathy.The ADCav variation precedes changes in routine MRI.

Objective To detect the histological characteristics of collagen nodules from hypertrophic scars (HS) and investigate the origin of collagen nodules.Methods The scar tissues were collected from patients with plastic operation.Morphological characteristics of collagen nodules were observed by light microscopy of HE-stained sections; expressions of type Ⅰ /Ⅲ collagens were observed by polarized light microscopy of sirius red-stained sections; expression and distribution of myofibroblasts (MFb)-specific protein (α-smooth muscle actin,α-SMA) were observed by immunostaining in order to observe level of local tissue tension.Results Collagen nodules varied in shape,not only sphereshaped,and in size.Moreover,abundant fibroblasts (Fb) with large and light-stained nuclei were seen in the nodules compared to non-nodule area,indicating that the cells located in the modules were active.Some collagen nodules were composed largely of collagen type Ⅲ (green),but some mainly contained collagen type Ⅰ (red or yellow),indicating the difference in the time of nodule formation.α-SMA was expressed mainly in the deep dermis equivalent to the level of reticular layer of the new scar tissues (2 months after injury) ; α-SMA was expressed mainly in the nodules of the old scar tissues (2-10 years after injury),but almost not in non-nodule areas except for a strongly positive staining in the vessels.Moreover,α-SMA presented a heterogeneous distribution in the collagen nodules,with stronger expression in the epidermal end than in the subcutaneous tissue end and stronger expression in the superficial dermis than that in the deeper part.It was suggested that there existed massive amount of MFb and high tension in the nodules arid that the tension distribution was not uniform in or between the nodules.Conclusions Collagen nodules are of varying shape and size and collagen types are associated with the time of nodule formation.Moreover,Formation of the collagen nodules may be closely related to the distribution and evolution of the local tissue tension.

Objective To study the adsorption performance of chitosan in printing and dyeing wastewater treatment.Methods The influence factors such as chitosan concentrations(0-500 mg/L),pH value(1-13),temperatures(20-50 ℃)and time(0.5-2.5 h) were considered in the test.Results When the concentration of chitosan was 200 mg/L,pH value was 2-5,time was 0.5 h and at the room temperature,the absorption could show a good result.The deeolorizing rate could reach above 90%.Conclusion The chitosan concentrations,pH value,time and temperature affect the adsorption performance of chitosan in printing and dyeing wastewater treatment.

Objective To optimize the extraction method of salviae miltiorrhizae madix et rhizoma in Huoluo-Xiaoling granules. Methods According to the contents of Salvianolic acid B and Tanshinone Ⅱ A, the extraction method was established by comparing different solvents (water and 70% ethanol)and extracting modes (compound extraction and single herb extraction). Then orthogonal design was used to determine the optimum extraction method. Results Considering the contents of salvianolic acid B and tanshinone Ⅱ A, 70% ethanol extract was better than water extract and compound extraction was better than single herb extraction.The optimum extraction condition was 70% ethanol in eight times of the herbs weight, extracted for 1h by 3times. Conclusion The extraction method was simple and stable.

Objective To analyze the species of the antibody and immune responsibility in pneumonic plague patients in order to pave the way to screen the new sub-unit of the vaccine to provide the experimental basis. Methods Using the virulence-related protein microarray containing 149 proteins of Yersinia pestis (Y.pestis), the species of the antibody and immune responsibility were analyzed in serum of two pneumonic plague patients in six months after onset. Results Eighty-eight gene coded proteins were detected out the related antibodies except YPMT1.23c, YPMT1.86, YPO0406 and YPO1071 in patient 1. Forty-three antibodies from gene coded protein were analyzed, other forty-nine had not been identified in patient 2. Thirty-nine antibodies were detected in both patients. The proteins YPMT1.81c, YPMT1.84, YPCD1.31c, rw10, YPCD1.28, YPCD1.58, YPMT1.62c, YPO3247-related antibodies increased significantly by 109.96,176.4 ;20.64,17.73 ;16.50,7.16 ;23.51,7.65 ;46.00,25.61 ;4.50,8.24 ;5.98,5.08 ;23.98,4.76 folds, respectively. Conclusions The study on the antibody in pneumonic plague patients helps us to select the potential vaccine candidates, which reveals that eight proteins are the immunity diagnosis targets and the research key of sub-unit vaccine.

From an aqueous extract of Lonicera japonica flower buds, sixteen compounds were isolated by a combination of various chromatographic techniques including column chromatography over macroporous resin, MCI gel, silica gel, and sephadex LH-20 and reversed-phase HPLC. Their structures were elucidated by spectroscopic data analysis as 6'-O-acetylvogeloside (1), 6'-O-acetylsecoxyloganin (2), dichlorogelignate (3), guanosinyl-(3' --> 5')-adenosine monophosphate(GpA,4) , 5'-O-methyladenosine (5), 2'-O-methyladenosine (6), adenosine (7), syringin (8), methyl 4-O-β-D-glucopyranosyl caffeate (9), (-)-dihydrophaseic acid 4'-O-β-D-glucopyranoside (10), ketologanin (11), 7α-morroniside (12), 7β-morroniside (13), kingiside (14), cryptochlorogenic acid methyl ester (15), and 6-hydroxymethyl-3-pyridinol (16). All the compounds were obtained from this plant for the first time, compounds 1 and 2 are new compounds, 3 and 5 are new natural products, and 4 is the first example of dinucleoside monophosphate isolated from a plant extract.
Chromatography, High Pressure Liquid ; Drugs, Chinese Herbal ; chemistry ; isolation & purification ; Flowers ; chemistry ; Lonicera ; chemistry ; Mass Spectrometry ; Molecular Structure

OBJECTIVETo investigate the efficacy, adverse reaction and prognosis of liquid nitrogen freezing combined with 5-aminolaevulinic acid-photodynamic therapy (ALA-PDT) in the treatment of condyloma acuminatum in men.

METHODSWe collected medical histories and conducted physical examinations for 35 male patients with condyloma acuminatum in the outpatient department, and treated them by liquid nitrogen freezing combined with ALA-PDT every 7-10 days. We recorded the skin lesions and adverse events each time and followed up the patients at 4 and 24 weeks after treatment.

RESULTSThe 35 patients were aged 17-71 (median 31) years, and their average course of disease was 30-180 (mean 60) days. The skin lesion area was reduced remarkably after 3 times of liquid nitrogen freezing combined with ALA-PDT, 16-140 (median 38) mm2 for the first time, 6-63 (median 13) mm2 for the second, and 0-10 (median 3) mm2 for the third, with statistically significant differences (P < 0.01). Two cases (5.7%) relapsed at 4 weeks and 4 cases (11.4% ) at 24 weeks.

CONCLUSIONLiquid nitrogen freezing combined with ALA-PDT is better than either liquid nitrogen freezing or ALA-PDT alone for the treatment of condyloma acuminatum in men.

Adolescent ; Adult ; Aged ; Aminolevulinic Acid ; administration & dosage ; therapeutic use ; Condylomata Acuminata ; therapy ; Cryotherapy ; Humans ; Male ; Middle Aged ; Photochemotherapy ; Prognosis ; Young Adult

AIMTo investigate the chemical components of Agelica sinensis.

METHODSChromatographic technologies were used for separation and purification, while spectral analysis was measured for structure elucidation.

RESULTSFive compounds were isolated and their structures were identified as Homosenkyunolide H (1), Homosenkyunolide I(2), Neoligustilide (3), 6-methoxycoumarin (4), Hypoxanthine-9-beta-D-ribofuranoside (5).

CONCLUSIONCompounds 1 and 2 are new compounds. Compounds 3, 4 and 5 were isolated from Agelica sinensis for the first time.

Angelica sinensis ; chemistry ; Benzofurans ; chemistry ; isolation & purification ; Molecular Conformation ; Molecular Structure ; Plants, Medicinal ; chemistry ; Stereoisomerism