Cardiopulmonary Resuscitation ; China ; Humans ; Time Factors

Animals ; Calreticulin ; metabolism ; Male ; Muscle, Skeletal ; metabolism ; Oxidative Stress ; Physical Conditioning, Animal ; Rats ; Rats, Sprague-Dawley

As a member of tyrosine kinases(TK) family,Epidermal growth factor receptor(EGFR) has an activity of intrinsic protein tyrosine kinase,which plays an essential role in the regulation of signal transduction in the cells.Due to the abnormal expression of EGFR-TK,the certain type cancers may developed and progressed.Based on that,the inhibitors of EGFR-TK could be effective medicines for the treatment of cancer.The EGFR-TK domain was amplified by RT-PCR with RNA of HUVCEs cells as the template and expressed in E.coliBL21(DE3) using plasmid pET30a as vector.The recombinant protein was purified with the affinity chromatography(Ni-NTA),which was identified to have kinase activity catalyzing the substrate phosphorylated with ATP in the enzymatic reaction.Using the recombinant EGFR-TK as target,the screening model for enzymatic inhibitors was constructed.

Jaundice can occur in most neonates.Most jaundice is benign,but beacuse of the potential toxicity of bilirubin,newborn infants with severe hyperbilirubinemia can develop acute bilirubin encephalopathy or kernicterus.In October 2004,American(academic) of pediatrics produced the latest guideline for newborn jaundice aim to reduce the incidence of acute bilirubin encephalopathy or kernicterus and avoid unnecessary costs or treatment.The latest guideline emphasizes the important of successful breastfeeding,time of jaundice developed,assessment for the risk of severe hyperbilirubinemia,close follow-up,and prompt intervention when indicated,provide a framework for the prevention and management of hyperbilirubinemia in newborn infants of 35 or more weeks of gestation.Some new view in the latest guideline may be helpful to us.

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In order to determine the challenge dose of pigeon paramyxovirus type 1 (PPMV-1) inactivated vaccine (S-1 strain). The virus titer of PPMV-1 E5 allantoic fluid (Chuansha strain) was determined using SPF chicken embryos in this research. After inoculating 30-day-old and 120-day-old pigeons with low-HI antibody against PPMV-1 (HI antibody < or =2) with different doses of PPMV-1 (Chuansha strain), the clinical symptoms and histopathological lesions of the challenged pigeons were examined. The results showed that the minimal lethal dose (MLD) of PPMV-1 (Chuansha strain) was 102.5 ELD50, so we determined that 10(5.5) ELD50, which was 1000 times the MLD, could be taken as the challenge dose in the vaccine efficacy test for PPMV-1 inactivated vaccine (S-1 strain).
Animals ; Antibodies, Viral ; immunology ; Bird Diseases ; immunology ; mortality ; virology ; Chick Embryo ; Columbidae ; immunology ; virology ; Newcastle Disease ; immunology ; mortality ; virology ; Newcastle disease virus ; immunology ; pathogenicity ; Phylogeny ; Viral Vaccines ; immunology ; Virulence

Apoptosis ; Cell Proliferation ; Gene Expression ; Gene Silencing ; HL-60 Cells ; Humans ; Intracellular Signaling Peptides and Proteins ; genetics ; RNA, Small Interfering ; genetics

Humans ; Infant, Newborn ; Infant, Premature ; Infant, Premature, Diseases ; microbiology ; prevention & control ; Mycoses ; prevention & control