1.X-ray analysis of lung damage caused by PCl3 inhalation.
Chinese Journal of Industrial Hygiene and Occupational Diseases 2005;23(4):308-309
Adult
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Chlorides
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toxicity
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Female
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Humans
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Inhalation Exposure
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Lung
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diagnostic imaging
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Lung Injury
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diagnostic imaging
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Male
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Middle Aged
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Phosphorus Compounds
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toxicity
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Radiography
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Young Adult
4.Clinical observation and follow-up study on acute promyelocytic leukemia in childhood treated mainly with arsenic trioxide.
Liang-chun HAO ; Hong WANG ; Li-zhong ZHANG
Chinese Journal of Pediatrics 2005;43(7):534-535
Antineoplastic Agents
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therapeutic use
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Arsenicals
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therapeutic use
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Child
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Child, Preschool
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Disease-Free Survival
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Female
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Follow-Up Studies
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Humans
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Leukemia, Promyelocytic, Acute
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drug therapy
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Male
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Oxides
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therapeutic use
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Remission Induction
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methods
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Survival Rate
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Treatment Outcome
5.Lentivirus-mediated shRNA interference targeting ADAM17 down-regulates MMP-9 expression in airway epithelial cells via TNF-α/NF-κB signaling
Jianping YAN ; Yaqing LI ; Hui ZHONG ; Chun CHEN ; Chao GU
Chinese Pharmacological Bulletin 2014;(4):559-565
Aim To study the role of TNF-α/NF-κB signaling in matrix metalloproteinase ( MMP)-9 expres-sion induced by lipopolysaccharide ( LPS ) in airway epithelial cells, and to investigate the effects of lenti-virus mediated RNAi targeting a disintegrin and metal-loproteinase 17 ( ADAM17 ) gene on MMP-9 expression induced by LPS. Methods The ADAM17 siRNA ex-pression vector was constructed, and packaged to re-combinant lentivirus in 293T cells. The HBE4-E6/E7 cells were pretreated for 30 min by NF-κB inhibitor ( PDTC) and a recombinant human TNFR p75-Fc fu-sion protein ( Etanercept) , or infected by the recombi-nant lentivirus for 72 h, and then stimulated for 24 h by LPS or TNF-α. The release of TNF-α was detected by ELISA. The mRNA and protein levels of MMP-9 were analyzed respectively by RT-PCR and Western blot. NF-κB activity was detected by electrophoretic mobility shift assay. Results LPS and TNF-α signifi-cantly increased MMP-9 mRNA and protein expressions and the activation of NF-κB in HBE4-E6/E7 cells ( P<0. 05 ) . Etanercept and PDTC significantly inhibited MMP-9 expression and the activation of NF-κB induced by LPS ( P<0. 05 ) . Lentivirus mediated RNAi targe-ting ADAM17 significantly decreased TNF-α produc-tion, inhibited MMP-9 mRNA and protein expressions and the activation of NF-κB induced by LPS in HBE4-E6/E7 cells ( P <0. 05 ) . Lentivirus mediated RNAi targeting ADAM17 did not inhibit MMP-9 mRNA and protein expressions and the activation of NF-κB in-duced by TNF-α ( P>0. 05 ) . And PDTC significantly inhibited MMP-9 mRNA and protein expressions and the activation of NF-κB induced by TNF-α ( P <0. 05 ) . Conclusions TNF-α/NF-κB signaling partic-ipates in the regulation of MMP-9 expression induced by LPS in airway epithelial cells, and lentivirus-media-ted RNAi targeting ADAM17 plays an important role in that signaling pathway upstream by regulating TNF-αrelease.
6.Application of a new operation mode of cholangioenterostomy.
Zhong LI ; Chun-Liang GUO ; Zhen-Yu HOU
Chinese Journal of Oncology 2010;32(12):946-947
Aged
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Aged, 80 and over
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Alanine Transaminase
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blood
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Bilirubin
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blood
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Choledochostomy
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methods
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Common Bile Duct
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surgery
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Common Bile Duct Neoplasms
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complications
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Drainage
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Female
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Follow-Up Studies
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Humans
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Jaundice, Obstructive
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blood
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etiology
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surgery
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Jejunum
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surgery
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Male
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Middle Aged
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Pancreatic Neoplasms
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complications
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Survival Rate
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gamma-Glutamyltransferase
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blood
7.Chemical constituents from endophyte Chaetomium globosum in Imperata cylindrical.
Li SHEN ; Li ZHU ; Zhong-qi WEI ; Xiao-wen LI ; Ming LI ; Yong-chun SONG
China Journal of Chinese Materia Medica 2015;40(23):4645-4649
Isolation and purification of chemical constituents from solid culture of endophyte Chaetomium globosum in Imperata cylindrical was performed through silica gel column chromatography, gel filtration over Sephadex LH-20 and preparative HPLC. Nine compounds were obtained and their structures were determined as chaetoglobosin F(1), chaetoglobosin Fex(2), chaetoglobosin E(3) cytoglobosin A(4), penochalasin C(S), isochaetoglobosin D (6), N-benzoylphenylalaninyl-N-benzoyphenylalaninate(7), uracil(8) and 5-methyluracil(9), respectively, based on HR-MS and NMR data and comparison with literatures. Compound 7 was isolated from Chaeeomium sp. for the first time. In vitro cytotoxicity of compounds was evaluated using MTT mothed and 1,3,4 and 5 showed inhibition activity to the human cervical carcinoma cell HeLa with IC50 values of 99.43, 23.77, 97.92, 86.25 micromol x L(-1), while positive cotolocisnin Ad apno1ch alse IC50 24.33 micromol x L(-1).
Biological Factors
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chemistry
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pharmacology
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Cell Line
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Cell Survival
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drug effects
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Chaetomium
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chemistry
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Endophytes
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chemistry
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Humans
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Molecular Structure
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Poaceae
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microbiology
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Spectrometry, Mass, Electrospray Ionization
8.Application of quantitative fingerprint to amino acids composition analysis of Xingnao Tongluo injection.
Jun-Hua HU ; Xia LIN ; Yan ZHONG ; Hui LI ; Jian-Xiong WU ; Jia-Chun LI ; Zhen-Zhong WANG ; Wei XIAO
China Journal of Chinese Materia Medica 2014;39(10):1811-1815
OBJECTIVETo control the quality of the product, quantitative fingerprint was used to evaluate the composition of the amino acids in the Xingnao Tongluo injection.
METHODThe method of the quantitative fingerprint to the amino acids composition was established through AccQ Tag precolumn derivatization. The quality was evaluated by the quantitative test of the amino acids and the similarity in ten batches.
RESULTThe Xingnao Tongluo injection contained 12 amino acids and the contents of these amino acids were stable. All the ten batches of the samples had similarity of more than 0.90.
CONCLUSIONThe method was accurate, feasible and could be a simple and effective way to evaluate the quality of the traditional Chinese medicine.
Amino Acids ; chemistry ; Chromatography, High Pressure Liquid ; methods ; Drugs, Chinese Herbal ; chemistry ; Quality Control
9.Growth activity of osteoblast on a novel strontium incorporated calcium sulfate.
Chun-Li ZHANG ; Yan-Tao ZHAO ; Shu-Xun HOU ; Hong-Bin ZHONG ; Zhong-Hai LI ; Yan LIU ; Ying ZHOU
China Journal of Orthopaedics and Traumatology 2014;27(5):415-418
OBJECTIVETo investigate the growth activity of osteoblast on a novel strontium incorporated calcium sulfate and make comparison with normal calcium sulfate material.
METHODSOsteoblast was inoculated on samples and cell proliferation was measured on the 1st, 3rd, 5th days, and the activities of ALP and osteocalcin were observed on the 5th day. And microcosmic morphology of osteoblast was observed by scanning electron microscopy(SEM).
RESULTSOsteoblast grows robustly on tested material. Cell quantity on the surface of novel material was obviously higher than normal calcium sulfate material (P < 0.05). The activity of ALP and osteocalcin on novel material was 57.8% and 40.2% higher than on normal calcium sulfate material respectively (P < 0.05). On strontium incorporated surface, osteoblast spread well. Cells were polygonal with abundant cytoplasm and the morphology was active.
CONCLUSIONStrontium incorporated calcium sulfate can sustain robust growth activity of osteoblast, which is promising to be used for bone substitute materials.
3T3 Cells ; Alkaline Phosphatase ; metabolism ; Animals ; Bone Substitutes ; chemistry ; pharmacology ; Calcium Sulfate ; chemistry ; pharmacology ; Cell Proliferation ; drug effects ; Mice ; Osteoblasts ; cytology ; drug effects ; metabolism ; Osteocalcin ; metabolism ; Strontium ; chemistry
10.Effect of probucol on adhesion of human monocytic line THP-1 induced by oxidiz ed low density lipoprotein
Chun LIANG ; Zonggui WU ; Zuo HUANG ; Renqian ZHONG ; Lingzhen ZHANG ; Li LI
Academic Journal of Second Military Medical University 2001;22(2):148-150
Objective: To determine the eff ect of probucol on adhesion of human monocytic line THP-1 induced by oxidized low density lipoprotein (oxLDL). Methods: THP-1 cells were induced by oxLDL in vitro. The CD11b, CD54 expressions and adhesion to human umbilic al vein endothelial cells (HUVEC) were measured after treatment with probucol at different concentrations by flow cytometry and β-nitrophenyl N-acetyl-β-D -glucosminide test. Results: Probucol inhibited the adhesion of oxLDL-induced THP-1 cells to HUVEC and down regulated the expression of CD11b in a dose dependent manner (P<0.01), but there was no inhibition on exp ression of CD54. Conclusion: Probucol can inhibit adhesion and a ggregation of monocyte-macrophages to endothelium in circulation, and may have anti-inflammatory action.