3.Relative factors of the compliance in schizophrenic out-patients
Chun-yang LI ; Ye LIANG ; Cui-ying MO ; Jiayi HUANG
Chinese Journal of Rehabilitation Theory and Practice 2006;12(1):77-78
ObjectiveTo explore the relative factors of the compliance in schizophrenic out-patients.Methods118 schizophrenic out-patients chosen randomly were investigate with the compliance and assessed with Clinical Global Impression-Severity of Illness (CGI-SI), Social Disability Screening Schedule (SDSS), Brief Psychiatric Rating Scale (BPRS) and Treatment Emergent Symptom Scale (TESS) by telephone. Logistic regression analysis was used to confirm the relative factors of the compliance.Results56.8% of all patients were of full compliance. The risk factors of compliance in schizophrenic out-patients were the frequency of medicine taken, the scores of CGI-SI and the insight of the illness. ConclusionIt is important to choose effective and convenient antipsychotic drugs and improve the insight of the illness to enhance the compliance of the schizophrenic out-patients.
4.Effect of zilongjin on immunologic function of patients with hepatocarcinoma in perioperative stage.
Qiang LI ; Qiang WU ; Hui-kai LI ; Yun-long CUI ; Chun-tao GAO
Chinese Journal of Integrated Traditional and Western Medicine 2005;25(2):163-164
Adjuvants, Immunologic
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therapeutic use
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Adult
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Aged
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B-Lymphocytes
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immunology
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CD4-CD8 Ratio
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Carcinoma, Hepatocellular
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drug therapy
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immunology
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surgery
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Drugs, Chinese Herbal
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therapeutic use
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Female
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Humans
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Killer Cells, Natural
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immunology
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Liver Neoplasms
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drug therapy
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immunology
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surgery
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Male
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Middle Aged
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Perioperative Care
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Phytotherapy
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T-Lymphocyte Subsets
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immunology
5.Determination of myclobutanil 25% WG degradation dynamics in ginseng root, stem, leaf and soil by HPLC-MS/MS.
Yan WANG ; Chun-Wei WANG ; Jie GAO ; Li-Li CUI ; Yun-Cheng XU
China Journal of Chinese Materia Medica 2014;39(13):2464-2468
A high performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) method was developed for determining degradation dynamics and final residues of myclobutanil 25% WG in ginseng root, stem, leaf and soil. The samples were extracted with acetonitrile, cleaned-up with primary secondary amine (PSA) solid phase extraction cartridge, separated by Kromasil Eternity-5-C18 (2.1 mm x 150 mm, 5 microm) column with a gradient of acetonitrile and 0.1% formate in water as mobile phases, and analyzed with the multiple reaction monitoring (MRM) in positive ion mode by employing the external standard method. The average recoveries and the relative standard derivations (RSDs) of myclobutanil at the spiked level of 0.01-0.20 mg x kg(-1) were 80.9%-90.7% and 5.54%-9.29%, respectively, and the limit of quantification (LOQ) was 0.005 mg x kg(-1). The method with good reproducible, high precision and low detection limit could meet the requirements of residual analysis on ginseng production. The half-lives of myclobutanil were from 6.25 days to 9.94 days in ginseng root, stem, leaf and soil at spraying dosage of 1 152 g x hm(-2) The final residues were below 0.060 1 mg x kg(-1) in root, below 0.081 7 mg x kg(-1) in stem, 0.006 0-0.102 2 mg x kg(-1) in leaf and below 0.037 6 mg x kg(-1) in soil at spraying dosage range from 576 to 1 152 g x hm(-2). It is recommended that the MRLs of myclobutanil in dried ginseng may be suggested to be 0.10 mg x kg(-1) temporarily, and the preharvest interval was set at 35 days.
Chromatography, High Pressure Liquid
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methods
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Drug Contamination
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Fungicides, Industrial
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chemistry
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Nitriles
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chemistry
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Panax
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chemistry
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Plant Leaves
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chemistry
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Plant Roots
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chemistry
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Plant Stems
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chemistry
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Soil Pollutants
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chemistry
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Spectrometry, Mass, Electrospray Ionization
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methods
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Tandem Mass Spectrometry
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methods
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Triazoles
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chemistry
6.Streptococcal toxic shock syndrome in a case.
Li-jie WANG ; Zhuang YUAN ; Chun-feng LIU ; Cui-zhi YAN ; Guang-xia ZHAO
Chinese Journal of Pediatrics 2003;41(12):939-939
7.Surveys on the implementation of "rules on stop smoking in the public places in Beijing" from 1997 to 2004.
Xiao-bo CUI ; Chun-yu LI ; Ying-sheng RAO
Chinese Journal of Epidemiology 2006;27(8):694-697
OBJECTIVETo find out the situation of the implementation on "rules on stop smoking in the public places in Beijing", a study was carried out to compare the results from 1997 and 2001.
METHODSThousands of residents aged above 15 in a family were sampled by stratified sampling, and 4 districts were selected as study locales.
RESULTS(1) About 40% of the department or school leaders considered much about the work on banning of smoking with 70 % of the departments or schools having posters of signs on 'ban on smoking' in the meeting rooms. (2) Some of the departments or schools had inspectors looking after the issue. (3) 60% of the people had never or 10% had seen that the inspectors ever criticizing or punishing the smokers who violating the rules. (4) People still often saw some one would smoke in public (the rates were 19.53% , 37.89% , 6.35% respectively in 1997,2001,2004 ). (5) People received the information of these rules through TV news or newspaper.
CONCLUSIONAlthough success had been achieved since the implementation of the Rules, it did not seem to have had substantial progress.
Adolescent ; Adult ; Aged ; China ; Data Collection ; Humans ; Middle Aged ; Public Policy ; Public Sector ; Smoking Prevention
8.Effects of integrinβ1 gene silencing on invasion of human pancreatic cancer cells PANC1
Feng YU ; Xuefeng BU ; Hua LI ; Yong-jun ZHANG ; Chun-you WANG ; Jing CUI
Chinese Journal of Pancreatology 2011;11(5):322-325
Objective To investigate the effects of integrin β1 gene expression inhibited by short hairpin RNA (shRNA) on invasion of pancreatic carcinoma PANC1 cells in vitro,and investigate the mechanism.Methods The eukaryotic expression plasmid of shRNA targeting integrin β1 gene ( integrin β1 shRNA) and control eukaryotic expression plasmid shRNA (c-shRNA) was constructed and was transfected into PANC1 cells.The cells without plasmid transfection were used as control.The expressions of integrinβ1,MMP 2,MMP 9 mRNA and protein were detected by real-time PCR and Western blotting.The invasive ability of PANC1 cells was observed with Transwell cell culture chamber.Results Integrinβ1 mRNA expressions in integrinβ1 shRNA group,c-shRNA group and control group were 0.0029 ± 0.0004,0.0131 ± 0.0009,0.0138 ± 0.0005 ; the expressions of integrinβ1 protein were 0.0159 ± 0.0062,0.3215 ± 0.0126,0.3107 ±0.0094; the inhibitory rate of integrinβ1 mRNA and protein expression in integrinβ1 shRNA group was (78.6 ±7.2 ) % and (92.9 ± 3.2) % ( P < 0.01 ).But there was no difference between the c-shRNA group and control group (P =0.2999).Number of penetrating cells in integrinβ1 shRNA group decreased from 52 ±5 to 21 ±4( P < 0.01 ) ; the expression of MMP 2 and MMP 9 mRNA decreased from 0.592 ± 0.073,0.847 ± 0.069 to 0.102 ± 0.034,0.273 ± 0.071 ; the expression of M MP2 and MMP 9 protein decreased from 0.225 ± 0.046,0.416 ±0.081 to 0.059 ±0.013,0.106 ±0.022(P <0.05).Conclusions Recombinant integrinβ1 shRNA expression plasmid can effectively inhibit the expression of integrinβ1 gene and suppress the invasion of PANC1 cells in vitro by down-regulating MMP 2 and MMP 9 gene expression.
9.Changes of CD4~+CD_(25)~+ Regulatory T Cells in Children with Autoimmune Diseases
li-ping, SUN ; chun-rong, SUN ; xiao-dai, CUI ; feng-qi, WU
Journal of Applied Clinical Pediatrics 2004;0(09):-
Objective Through detecting CD4+CD25+ T regulatory cells(Treg)in the peripheral blood in children suffering autoimmune diseases and normal controls to learn about the changes of Tregs during the diseases and to acquire some references for clinical diagnosis and treatment.Methods The data were reviewed for CD4+CD25+ Treg cells of the 93 children diagnosed as pediatric autoimmune disease in Children′s Hospital Affiliated to Capital Institute of Pediatrics from Nov.2007 to Jun.2008.Thirty-five normal children in the contempora-neous physical examination were selected as the control group.The percentage of CD4+CD25+ Treg cells and CD4+ T cells to the total T cells were determined by flow cytometric method.Data of the JRA group(22 cases),SLE group(12 cases) and HSP group(12 cases),which were the first three according to the number of cases,were respectively compared with the controls.Independent-samples t test was performed for a statistic analysis with SPSS 11.5 software.Results 1.The percentages of CD4+CD25+ Treg cells to the total T cells and CD4+ T cells in the autoimmune diseases children[(6.14?3.21)% and(21.85?11.68)%,respectively] were both higher than those in the control group[(3.68?1.02)% and(12.83?3.61)%,respectively Pa
10.The roles of important molecules of Wnt signaling pathway in non-small-cell lung cancer.
Chun-yan LI ; Ze-shi CUI ; Yao LU ; Ying ZHANG ; Jian GAO ; En-hua WANG
Chinese Journal of Pathology 2005;34(9):599-600
Carcinoma, Non-Small-Cell Lung
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metabolism
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pathology
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Cell Membrane
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metabolism
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Cell Nucleus
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metabolism
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Cytoplasm
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metabolism
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Humans
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Lung Neoplasms
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metabolism
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pathology
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Signal Transduction
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TCF Transcription Factors
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metabolism
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Transcription Factor 7-Like 2 Protein
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Wnt Proteins
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physiology
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beta Catenin
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metabolism