1.Extraventricular neurocytoma of spinal cord: report of a case.
Chun-nian WANG ; Xiang-lei HE ; Zhao-xia XIA
Chinese Journal of Pathology 2012;41(10):702-703
Antigens, Nuclear
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metabolism
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Cordotomy
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methods
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Diagnosis, Differential
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Ependymoma
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metabolism
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pathology
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Follow-Up Studies
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Humans
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Male
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Middle Aged
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Nerve Tissue Proteins
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metabolism
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Neurocytoma
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metabolism
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pathology
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surgery
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Oligodendroglioma
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S100 Proteins
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metabolism
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Spinal Cord Neoplasms
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metabolism
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pathology
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surgery
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Synaptophysin
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metabolism
3.Optimized alloxan-induced diabetic rabbit model
Wen-Tao, SUN ; Chun-Ling, LEI ; Si-Hai, ZHAO ; Chun-Chao, BI ; Lu, ZHANG
International Eye Science 2010;10(10):1848-1850
AIM: To explore the frequency of drug injection of alloxan diabetes on the established model of rabbit.METHODS: Thirty-six healthy rabbits, weighing 2-2.5kg, were randomly divided into one time drug injection group (group A, n=12), two times drug injection group (group B, n=12) and three times drug injection group (group C, n=12). Each rabbit was injected with a total amount of 150mg/kg of alloxan. Fasting blood glucose was measured. The success rate and death rate of each group were also calculated.RESULTS: The success rate of diabetic rabbit model in group B was higher than that in group A (P<0.01) and its death rate was lower than that of group A (P<0.01); the success rate of diabetic rabbit model in group C was highest and the death rate was the lowest in three groups(P<0.01). CONCLUSION: Multiple administration of alloxan can improve success rate in establishing diabetic rabbit model with decreased death rate and increased stability.
4.Studies of Temperature Shift Fermentation for Eicosapentaenoic Acid Production by Nitzschia laevis
Xiao-Hong CAO ; Yu-Hua ZHAO ; Mei-Fang LU ; Jing LEI ; Chun-Ling WANG ;
China Biotechnology 2006;0(12):-
Fermentation for Eicosapentaenoic Acid(EPA) production by Nitzschia laevis at various temperature between 10℃ and 30℃ was investigated and the dynamics characteristics during fermentation process were also analyzed.Based on the results,a varying temperature nursing method of two stage control strategy is proposed:During the first stage,which comprises the delay phase and the initial index phase,the temperature is maintained at 25℃;then the temperature is shifted to 20℃ and kept up till the end of the fermentation process.By this method,a EPA content of 6.0% and a yield of 291.60 mg/L have been gained.These are 24.07% and 18.81% higher than that of fixed temperature(25℃) fermentation,respectively.
5.Primary observing pulmonary function variety following three-dimensional conformal radiation therapy of Ⅲ phase non-small cell lung cancer
Feng CAI ; Guang LI ; Yu-Xia ZHAO ; Jun DANG ; Lei YAO ; Chun-Li WU ;
Chinese Journal of Radiation Oncology 2005;0(06):-
Objective To explore the pulmonary function,DVH and radiation pneumonitis after three-dimensional conformal radiation treatment ofⅢphase non-small-cell lung cancer.Methods 71 pa- tients (male 52,female 19,median age 63,KPS≥80) were evaluated by pulmonary function tests before radiotherapy and in M1 and M3 after radiotherapy respectively.After 3 months of follow-up time,it reviewed the appearance and grade of radiation pneumonitis.Then V_(20),V_(30) and MLD were worked out from dose vol- ume histogram.Results All patients completed radiotherapy,and total dose was 66-70 Gy.FVC (L), FEV1 (L) and CLCO were (2.58?0.65) L,(1.85?0.58) L and (15.15?4.65)ml/(min)before radio- therapy,with (2.96?0.76) L,(2.13?0.65) L and (14.71?3.92) ml/(min) in Ml after radiotherapy, with (2.65?0.61) L,(1.92?0.52) L and (13.15?3.71)ml/(min)in M3 after radiotherapy.The ac- cidence of radiation pneumonitis was 30%,moderate and severe radiation pneumonitis was 7%.With V_(20), V_(30) and MLD increasing,the grade of radiation pneumonitis was increasing.V_(20),V_(30) and MLD were related to the change in CLCO value among before,M1 and M3 after radiotherapy,and the correlation coefficient was more than 0.2.Conclusions There is a relationship in the pulmonary function,DVH and radiation pneu- monitis surely.The change in C_LCO value between before radiotherapy and M1 after radiotherapy could pre- dict the radiation pneumonitis.V_(20),V_(30) and MLD are not only correlated to radiation pneumonitis evidently but the change in FEV_1 and C_LCO after radiotherapy.
6.Repeated injection of mitoxantrone containing thermosensitive liposomes in rat induced ABC phenomenon.
Wei TIAN ; Lan ZHANG ; Na WEI ; Chan LI ; Bei-Bei NI ; Xi ZHAO ; Chun-Lei LI
Acta Pharmaceutica Sinica 2014;49(2):256-259
To investigate whether accelerated blood clearance (ABC) phenomenon could be induced after repeated injection of mitoxantrone thermosensitive liposomes, LC-MS/MS and enzyme linked immunosorbent assay (ELISA) were used to measure the concentration of mitoxantrone and the anti-polyethylene glycol (PEG) IgM levels in rat plasma, separately. The drug was rapidly cleared away after the second administration. The anti-PEG IgM was detected after the first dose which was neutralized quickly after the second dose. It is proved that repeated administration of mitoxantrone thermosensitive liposomes in rat caused the ABC phenomenon.
Animals
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Antineoplastic Agents
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administration & dosage
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blood
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pharmacokinetics
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Chromatography, High Pressure Liquid
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Immunoglobulin M
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blood
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Liposomes
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administration & dosage
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blood
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pharmacokinetics
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Male
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Metabolic Clearance Rate
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Mitoxantrone
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administration & dosage
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blood
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pharmacokinetics
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Polyethylene Glycols
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administration & dosage
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chemistry
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pharmacokinetics
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Rats
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Rats, Wistar
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Spectrometry, Mass, Electrospray Ionization
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Tandem Mass Spectrometry
7.Flavonoids from Artocarpus hypargyreus.
Xin QIAO ; Ting ZHAO ; Meng WANG ; Chun LEI ; Aijun HOU
China Journal of Chinese Materia Medica 2011;36(21):2975-2979
OBJECTIVETo investigate flavonoids from Artocarpus hypargyreus.
METHODThe compounds were isolated by various chromatographic methods and identified by spectroscopic analysis.
RESULTTen compounds were isolated and their structures were identified as artohypaflavone (1), brosimone H (2), artonin A (3), artocarpin (4), artopetelin B (5), (-)-epiafzelechin (6), oxyresveratrol (7), (+)-afzelechin (8), (+)-catechin (9), and (+)-afzelechin-3-O-alpha-L-rhamnopyranoside (10).
CONCLUSIONCompound 1 is a new isoprenylated flavone, while compounds 2, 4-6, and 8 were isolated from this plant for the first time.
Artocarpus ; chemistry ; Drugs, Chinese Herbal ; chemistry ; isolation & purification ; Flavonoids ; chemistry ; isolation & purification ; Molecular Structure
8.Content of mineral elements of Gastrodia elata by principal components analysis.
Jin-ling LI ; Zhi ZHAO ; Hong-chang LIU ; Chun-li LUO ; Ming-jin HUANG ; Fu-lai LUO ; Hua-lei WANG
China Journal of Chinese Materia Medica 2015;40(6):1123-1128
OBJECTIVETo study the content of mineral elements and the principal components in Gastrodia elata.
METHODMineral elements were determined by ICP and the data was analyzed by SPSS.
RESULTK element has the highest content-and the average content was 15.31 g x kg(-1). The average content of N element was 8.99 g x kg(-1), followed by K element. The coefficient of variation of K and N was small, but the Mn was the biggest with 51.39%. The highly significant positive correlation was found among N, P and K . Three principal components were selected by principal components analysis to evaluate the quality of G. elata. P, B, N, K, Cu, Mn, Fe and Mg were the characteristic elements of G. elata.
CONCLUSIONThe content of K and N elements was higher and relatively stable. The variation of Mn content was biggest. The quality of G. elata in Guizhou and Yunnan was better from the perspective of mineral elements.
Drugs, Chinese Herbal ; analysis ; Gastrodia ; chemistry ; Minerals ; analysis ; Principal Component Analysis
9.Differential diagnosis of hyperdensities on computed tomography immediately after intra-arteriai thrombolysis in patients with acute ischemic stroke
Jun-Gong ZHAO ; Ming-Hua LI ; Chun FANG ; Ju WANG ; Pei-Lei ZHANG ; Zhuo-Ying DU ; Min LI ;
Journal of Interventional Radiology 1994;0(03):-
Objective The present study was to differentiate the hyperdensities on CT immediately after intra-arterial thrombolysis in patients with acute isehemic stroke.Methods Twenty two patients with acute ischemic stroke were treated with intra-arterial combining with intravenous thrombolysis within 6 hours after onset.All patients underwent nonenhaneed CT scans before,immediately and 24 hours after thrombolytic therapy.The hyperdensities on CT after intra-arterial thrombolysis were analyzed retrospectively.Results Five hyperdense areas were seen in 22 patients immediately after thrombolytie therapy.According to their locations, CT values and follow-up CT scans,the hyperdensities on CT imaging were classified into two groups:contrast enhancement and hemorrhagic transformation.The former was characterized by rapid clearance of the hyperdensity lesion with maximum Hounsifild Unit<90,on the contrary,the latter was noted by persistence of hyperdensity lesion after 24 hours CT scan with maximum Hounsifild Unit>90.Two of the five hyperdense lesion patients were confirmed to be contrast enhancement with location in cerebral cortex,the other were hemorrhagic transformation,mostly located in basal ganglia.Hyperdensity in patients with contrast enhancement showed neurological improvement although no further medical cares were offered.Conclusions Different kind of hyperdensity on CT immediately after intra-arterial thrombolysis in patients with acute ischemic stroke can be differentiated according to its location,CT value and follow-up CT scan.When contrast enhancement occurred,no further medical care is needed.
10.The study on protective effect of sphingosine-1-phosphate in cardiomyocytes.
Lei YUAN ; Wen-jie ZHANG ; Duo-duo ZHANG ; Chun-yan ZHAO
Chinese Journal of Applied Physiology 2011;27(3):320-323
OBJECTIVETo explore the mechanism of protective effect of Sphingosine-1-phosphate(S1P) in cultured neonatal rat cardiomyocytes dining simulated hypoxia/reoxygenation.
METHODSOn the basis of culturing neonatal rat cardiomyocytes, the model of hypoxia-reoxygennation was built by using method of Liquid Paraffin covering, the impact of S1P on apoptosis and p-Akt and mitochondrial membrane potential were studied by using method of Propidine Iodide staining and Western blot and Bhodanmine123 staining.
RESULTSSiP could reduce apoptosis rate (P < 0.01) and stabilize the mitochondrial membrane potential (P < 0.05) and improved the level of p-Akt1 (P < 0.01) in hypoxia/reoxygenation cardiomyocytes significantly. But wonnannin could block these effects of S1P partially.
CONCLUSIONSiP can obviously restrain apoptosis in curtured rat neonatal cardiomyocytes during simulated hypoxia/reoxygenation. Stabilization of mitochondrial membrane potential by P13K-AM signaling pathway is likely to play a role in protective action of S1P.
Animals ; Animals, Newborn ; Apoptosis ; drug effects ; Cell Hypoxia ; Cells, Cultured ; Female ; Lysophospholipids ; pharmacology ; Male ; Membrane Potential, Mitochondrial ; drug effects ; Myocardial Reperfusion Injury ; prevention & control ; Myocytes, Cardiac ; cytology ; Oncogene Protein v-akt ; metabolism ; Phosphatidylinositol 3-Kinases ; metabolism ; Primary Cell Culture ; Protective Agents ; pharmacology ; Rats ; Signal Transduction ; Sphingosine ; analogs & derivatives ; pharmacology