2.Brief analysis on academic experience and characteristics of tendon trauma of SUN Shu-chun
Dongxin TANG ; Jun ZHANG ; Chun ZHANG ; Zhen WU ; Dong YU
China Journal of Traditional Chinese Medicine and Pharmacy 2005;0(12):-
SUN Shu-chun,the chief researcher,is one of the famous expert of osteo-traumatology of TCM.He makes systematic sort and study on chirismus on basis of succeeding experience predecessors and his own years of clinic experience.He is good at treating cervical syndrome with TCM chirismus,especially combine it with drugs.In osteo-traumatology academic circles,he is called "the best doctor at home".Professor SUN advocates that the rule of applying various therapies according to different conditions,combination of rigid-flexible,light,skill,gentle,harmony should be obey when we use chirismus.His diagnostic and therapeutic methods and thoughts are original and ingenious.In this article,the author analyzes the academic experience of tendon trauma of professor SUN.
3.Changes in liver function and immune function following laparoscopic and traditional open cholecystectomy: a comparative study.
Journal of Southern Medical University 2009;29(12):2495-2496
OBJECTIVETo compare the effect of laparoscopic cholecystectomy (LC) and open cholecystectomy (OC) on liver function and immune function.
METHODSForty-two patients with normal liver function underwent LC (n=21) or OC (n=21) according to the patients' preference. One day before and 7 days after the operations, the liver functions (ALT, AST, TBIL, ALB) and immune functions (CD3, CD4, CD8, NK cell percentage, IgA, IgM, IgG and C3, C4) of the patients were measured.
RESULTSNo statistical differences were found in the liver functions or immune functions between the two groups after the operation.
CONCLUSIONLC and OC show no significant difference in the effects on the liver function and immune function.
Adult ; Antibody Formation ; immunology ; Cholecystectomy ; Cholecystectomy, Laparoscopic ; Cholelithiasis ; immunology ; physiopathology ; surgery ; Female ; Gallbladder Neoplasms ; immunology ; physiopathology ; surgery ; Humans ; Immunity ; Killer Cells, Natural ; immunology ; Liver ; physiopathology ; Liver Function Tests ; Male ; Middle Aged ; Polyps ; immunology ; physiopathology ; surgery
4.Protection, exploitation and utilization states of specialized Mongolian folk medicine resources and related development strategy.
Chun-hong ZHANG ; Da MAN ; Guo-dong WU ; Zhen-hua LI ; Dong-dong ZHAO ; Yong LIU ; Min-hui LI
China Journal of Chinese Materia Medica 2015;40(5):771-777
Mongolian folk medicine resource is the origin of Mongolian medicine development, even more important of which is the specialized Mongolian folk medicine resources with regional and high medicine quality, it processes distinctive national characteristics with irreplaceable important position in traditional Mongolian medicine. Nevertheless, due to the serious destroy of ecological environment and sharp increase of demands, etc. A lot of specialized Mongolian folk medicine resources were endangered, and there still existed some problems in the protection and exploitation and utilization. This paper intends to provide comprehensive insight into the species protection and exploitation and utilization states of specialized Mongolian folk medicine resources. The application and protection status and the existing problems were reviewed, and the development strategies of Mongolian folk medicine resource were analyzed.
Conservation of Natural Resources
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methods
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Environment
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Medicine, Mongolian Traditional
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Mongolia
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Plants, Medicinal
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classification
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growth & development
6.Effects of mifepristone on protection of progesterone against oxygen-glucose deprivation injury in PC12 cells.
Chun-Ping WU ; Guo-Hong WANG ; Yong ZHANG ; Dong-Liang LI
Chinese Journal of Applied Physiology 2013;29(2):132-134
Animals
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Cell Hypoxia
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Glucose
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metabolism
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Mifepristone
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pharmacology
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Oxidative Stress
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PC12 Cells
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Progesterone
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pharmacology
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Rats
7.Treatment of HBeAg positive chronic hepatitis B by xiaoyao powder combined with interferon-alpha: a clinical observation.
Hui-qing LIANG ; Jin-mo TANG ; Chun-cheng WU ; Shao-dong CHEN
Chinese Journal of Integrated Traditional and Western Medicine 2014;34(6):666-670
OBJECTIVETo study the efficacy of Xiaoyao Powder (XYP) combined with interferon alpha (IFN-alpha) in treating HBeAg positive chronic hepatitis B (CHB) patients and the effect on their quality of life (QOL).
METHODSTotally 193 patients with HBeAg-positive CHB confirmed by liver biopsy were randomly assigned to 2 groups, Group A (94 cases) and Group B (99 cases). IFN-alpha1b was subcutaneously injected to patients in Group A at the dose of 50 microg, thrice per week. Those in Group B additionally took XYP. The therapeutic course for all was 24 weeks. Clinical efficacy was observed by assessing ALT restoration rate, HBeAg negative rate, HBeAg conversion rate, HBV DNA negative rate, complete response rate, partial response rate, and symptoms integral. The evaluation of QOL was performed by using chronic liver disease questionnaire (CLDQ) score. Adverse reaction occurrence rate was observed in the two groups.
RESULTSBetter effects were obtained in Group A on ALT restoration rate, HBeAg negative rate, HBV DNA negative rate, complete response rate, partial response rate, TCM symptoms integral, the total effective rate of TCM sysmptoms, CLDQ score, and adverse reaction rates, showing statistical difference when compared with Group B (P < 0.05, P < 0.01).
CONCLUSIONXYP could elevate the efficacy of TCM symptoms of HBeAg-positive CHB patients and anti-viral effect, improve their QOL, and reduce adverse reaction of IFN-alpha.
Adult ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Hepatitis B e Antigens ; Hepatitis B, Chronic ; drug therapy ; Humans ; Interferon-alpha ; therapeutic use ; Male ; Quality of Life ; Treatment Outcome
8.Foam cells can be induced by oxidized low density lipoprotein in human monocyte-derived dendritic cells
Chun LIANG ; Yukun LUO ; Dong HUANG ; Qingzhe JIA ; Congfeng XU ; Keqiang WANG ; Zonggui WU ; Junbo GE
Chinese Journal of Pathophysiology 2000;0(10):-
AIM: The purpose of this study is to investigate the mechanisms related to oxidized low-density lipoprotein(ox-LDL) and dendritic cells(DCs) in the process of atherosclerosis.METHODS: Human DCs were prepared from human CD14~+ peripheral blood monocytes using rhGM-CSF((100 ?g/L)) and rhIL-4(40 ?g/L).Cells were incubated with(100 mg/L) native or oxidized LDL for 72 h.The formation of foam cells was investigated by electron microscopy and oil red O staining.Phenotypic and immune functional assays were used with FACS,FITC-dextran phagocytosis,allogeneic mixed T lymphocytes reaction and secretion of Th1/Th2(IL-12/IL-2) cytokines were also conduced.RESULTS: DCs treated with ox-LDL,but not native LDL were induced into foam cells after cultured for 72 h.Compared with native LDL,ox-LDL-treated DCs were less potent in FITC-dextran phagocytosis.ox-LDL promoted allogeneic T cells proliferation.Moreover,ox-LDL upregulated CD80(72.4? 9.6 vs 89.5?10.1,P
9.Effects on osteoblast and adipocyte differentiation of bone marrow mesenchymal stimulated by pulsed electromagnetic fields
Yong YANG ; Hua WU ; Dong-Ming ZHAO ; Wen-Chun ZHAO ; Run-Guang LI ;
Chinese Journal of Physical Medicine and Rehabilitation 2003;0(08):-
Objective To study the effects of pulsed electromagnetic fields(PEMFs)of 15Hz and lmT on osteoblast differentiation of bone marrow mesenchymal stem cells.Methods The bone marrow mesenchymal stem cells were isolated and cultured in vitro,the third passage cells were harvested and half of them was exposed to PEMFs,another half exposed to sham PEMFs served as the control.The activity of ALP was measured.And the ex- pression of osteoblast and adipogenesis was measured with the semi-quantitative RT-PCR,adipogenic differentiation was observed with the Oil Red O Staining.Results When compared with the control,PEMFs of 15 Hz and 1 mT significantly increased the activity of ALP(P
10.Design and Functional Investigation of a Novel Anti-coagulative Fusion Protein by Hirudin With a Recognizing Sequence of FXa
Jin-Yang NIU ; Chun-Na DONG ; Ji-De JIN ; Bing-Xing SHI ; Zu-Ze WU ;
China Biotechnology 2006;0(04):-
Hirudin (HV) is known as the most potent and specific inhibitor of thrombin. Although hirudin has many advantages , it has the bleeding side effect and this is the great shortage of hiudin for clinical application. In order to alleviate bleeding side effect of hirudin, fusion protein, named as FHV (fusion hirudin linked with FXa recognition peptide) was designed. The fusion protein gene ( fhv) was cloned into plasmid pPIC9K. FHV engineered Pichia pastoris containing high copies was chosen for fermentation and purification at 30 L fermentor scale, finally, FHV with purity of above 97% was obtained. To investigate the function of FHV in vivo, mouse tail thrombosis model was used. In the mice thrombus tail model induced by carrageenan, FHV decreased the length of tail thrombus significantly, similar to that of HV control, and had no obvious effects on the TT, PT and APTT. In conclusion, FHV is constructed and expressed in yeast. FHV fusion proteins is obtained by fermentation and purification. FHV has antithrombotic effects not influencing IT, PT and APTT after administration immediately in animal models. Therefore, FHV is a promising anticoagulant and antithrombotic drug.