OBJECTIVE:To establish a method for the bacterial endotoxin test of chlorphenamine maleate injection. METHODS: The interference test and bacterial endotoxin test of 28 batches of chlorphenamine maleate injections from 14 manufacturers were carried out in accordance with the bacterial endotoxin test approved by Chinese Pharmacopeia (2005 edition). RESULTS: Diluted to a concentration of below 0.025 mg?mL-1,the samples showed no interference action on bacterial endotoxin test. The limit value of the bacterial endotoxin was 5 EU?mg-1. The results of the bacterial endotoxin test of the 28 batches of samples were all accorded with the standard of the established quality control. CONCLUSION: The established TAL method is applicable for bacterial endotoxin test of chlorphenamine maleate injection.

Objective To detect the changes in cTnI and CK MB during extracorporeal circulation and the myocardial injury by extracorporeal circulation. Methods The value of cTnI and CK MB was determined in 30 persons during extracorporeal circulation. We divided them into 6 groups according to pre operation, 6, 12, 24, 48 and 72 hours after operation. Modified method (Bodor et al) was used to detect cTnI, and immune suppression method to detect CK MB. Results cTnI and CK MB were, cTnI ( ?s ) (5.20?2 80) ?g/L,CK MB ( ?s ) (10.75?4.70) U/L in 28 persons. They elevated 6 hours after operation. 12 24 hours after operation cTnI and CK MB increased to the maximum, cTnI 28.80 71.30 ?g/L,CK MB 55.60 71.30 U/L. During 48 72 hours, CK MB decreased to normal, 49.70 22.80 U/L. cTnI was 9.40 19.80 ?g/L 72 hours after operation. Conclusion Extracorporeal circulation may cause myocardial diseases. cTnI is a more sensitive biochemical marker for myocardial diseases.

Objective To explore the mechanism of apeptosis in rheumatoid arthritis synoviocyte induced by dihydroartemisinin. Methods Synovial tissues were cut from rheumatoid arthritis patients when who was under knee prosthesis. Apoptosis was detected with flow cytometry. Western blot was performed to assess ser473-phosphorylated Akt. EMSA (electrophoretic mobility shift assay) was used to ana-lyze NF-κB activation. Results Dihydroartemisin can induce apoptosis in rheumatoid arthritis synoviocyte in a dose-dependent manor from 2.5μmol/L to 10μmol/L. Rheumatoid Arthritis synoviocyte cultured with dihydroartemisinin in 5μmol/L or 10μmoL/L can significantly in-hibit serine 473 phosphorylation in Akt and activation of NF-κB. Conclusion Dihydroartemisinin can induce apoptosis in rheumatoid arthri-tis synoviocyte through Akt signal pathway.

Objective To explore the feasibility, safety and advantage of thoracoscopes pulmonary bulla resection without thoracic closed drainage tube according to the fast track surgery (FTS) theory. Methods The clinical data of 53 patients with pulmonary bulla were retrospectively analyzed. Among them, 29 patients underwent the thoracoscopes pulmonary bulla resection without thoracic closed drainage tube (FTS group), and 24 patients underwent the thoracoscopes pulmonary bulla resection with thoracic closed drainage tube (tradition group). The oxygenation index at 24 h after operation, visual analogue score (VAS) at 24, 48 and 72 h after operation, postoperative complications, postoperative hospital stay and hospital costs were compared between 2 groups. Results There was no statistical difference in oxygenation index at 24 h after operation between FTS group and tradition group:(312.4 ± 16.4) mmHg (1 mmHg=0.133 kPa) vs. (330.6 ± 13.2) mmHg, P>0.05. The VAS at 24, 48 and 72 h after operation in FTS group was significantly lower than that in tradition group:(2.6 ± 1.1) scores vs. (4.3 ± 1.6) scores, (1.6 ± 0.9) scores vs. (3.8 ± 1.4) scores and (0.8 ± 1.0) scores vs. (2.9 ± 1.4) scores, and there was statistical difference (P<0.05). The incidence of postoperative complications, postoperative hospital stay and hospital costs in FTS group were significantly lower than those in tradition group:13.8%(4/29) vs. 37.5% (9/24), (3.2 ± 0.9) d vs. (6.2 ± 1.2) d and (1.3 ± 0.3) × 104 yuan vs. (1.5 ± 0.4) × 104 yuan, and there were statistical differences (P<0.05). Conclusions FTS used in thoracoscopes pulmonary bulla resection is feasible and safe, and has certain advantages.

OBJECTIVE:To establish a method for the bacterial endotoxin test of nicardipine hydrochloride glucose injection.METHODS:The bacterial endotoxin test was carried out according to the bacterial endotoxin test in the addenda of China Pharmacopeia 2005 edition(second part).RESULTS:Nicardipine hydrochloride glucose injection showed interference on the bacterial endotoxin test when water was taken as solvent.The preliminary interference test and interference test revealed that using thinnerⅠas solvent,the sample injection in over 4-fold dilution had no interference on the bacterial endotoxin test.CONCLUSIONS:It is feasible for bacterial endotoxin test of nicardipine hydrochloride glucose injection be conducted in a limit value of 0.5 EU?mL-1.The bacterial endotoxin text can substitute pyrogen test in the detection of nicardipine hydrochloride glucose injection.

Objective To investigate airway inflammation,number of eosinophils in blood and BALF,the expression of inhibitor protein ?(I ?B ?) and nuclear factor-?B(NF-?B) activity in lung of rat with spleen deficiency asthma,and the effect of herbs of reinforcing spleen to replenishing Qi.Method Thirty rats were randomly divided into three groups:control group(group A),spleen deficiency asthma group(group B),decoction group(group C).The pathological changes of lung was detected by HE staining,and the expression of I ?B ? and activity of nuclear factor ?B p65(NF-?B p65) was assessed in lung by immunohistochemistry.Results Compared with group A,the expression of I ?B ? significantly reduced(P

Objective:To observe the changes of visual evoked potential(VEP)in diabetic rats and the influence of nerve growth factor(NGF)and aminoguanidine(AG)on VEP.Methods:Diabetes was induced in adult male Wistar rats with streptozotocin(STZ).Rats were divided into normal control group(CON),diabetes model group(DM).NGF-treated group(D +N)and AG-treated group(D+A).VEP was measured during the 3~(rd)month,6~(th)month,9~(th)month,and 12~(th)month.Results: Compared with the CON group,all rest groups had longer latencies and lower amplitudes(P

Abstract?AIM: To observe the clinical efficacy of intravitreal Lucentis injection combined with panretinal photocoagulation ( PPR ) and compound trabeculectomy for neovascular glaucoma ( NVG) .?METHODS:A total of 14 cases (14 eyes) with NVG were collected from January to November 2015.All cases were treated with intravitreal lucentis injection, PPR and compound trabeculectomy by turns.Intraocular pressure ( IOP) , visual acuity and the complications at pre-or post-surgery were recorded, respectively.?RESULTS: Followed up for 3-6mo, the average IOP preoperatively was significantly decreased than that detected at post-operation ( 18.00 ±6.70 vs 41.65 ± 4.07mmHg, t=11.288, P<0.05).IOP less than 21mmHg with or without the usage of anti-intraocular pressure drugs was defined as the sign of successful or effective surgery, respectively.At the ultimate follow-up, 11 cases were successful, 2 cases were effective, and the success rate was 79%, effective rate was 14%.Only 1 case was applied cyclocryotherapy due to the uncontrolled IOP. Moreover, the results of visual acuity detection demonstrated that 6 eyes got a better visual acuity, 7 eyes remained the same condition and 1 case got no light perception. Meanwhile, 13 cases showed none iris neovascularization during the follow-up; 1 case got a reappearance of iris neovascularization on the third month, which was then dissolved subjected to the intravitreal lucentis injection in combination with PPR. One case developed post -operative hyphema and absorbed after 1wk. No shallow anterior chamber and eyeball atrophy happened.?CONCLUSION:Intravitreal lucentis injection combined with PPR and compound trabeculectomy is an effective and safe therapeutic strategy for the treatment of NVG.

Objective To investigate the characteristics of children′s recurrent abdominal pain(RAP) with gastroscopy and analyze the etiology.Methods Three hundred and thirteen children were investigated with gastroscopy.Gastric antrum mucosa was taken for histo-pathology and the determination of helicobacter pylori(Hp) antibody.Results Ninty-nine point six eight percent of the cases had lesions under the gastroscope.The former 4 cases had chronic superficial gastritis(CSG),CGS and bile reflux,CGS and duodenitis,CGS and bulb ulcer,and Hp infection rate was 31.36%,25%,38.64%,60.61%.Hp infection rates of active gastritis and inactive gastritis were 92% and 23.19%,which showed significant differences in 2 groups(P

Objective To establish rat models with Alzheimer's disease(AD) induced by ?-amyloid,and to observe the effects of nicotine on learning and memorizing ability of the rats. Methods Aggregated A?1-40 was injected into the bilateral basal forebrain of the rats.Learning and memorizing ability of the rats were inspected through Morris water maze test.The effects of nicotine on learning and memorizing ability of the rats induced by ?-amyloid peptide were observed. Results The learning and memorizing ability of AD animal models injected aggregated(A?1-40) for 2 weeks decreased(P