2.Pediatric Drug Policies in the USA and Its Illumination for China
China Pharmacy 2007;0(28):-
OBJECTIVE:To provide reference for the enactment of policies related to pediatric drug development in China.METHODS:The pediatric drug policies of the USA and its impact were studied comprehensively.RESULTS & CONCLUSIONS:The enforcement and stimulation policies were adopted in US for pediatric drug development,which achieved certain efficacy.China might as well draw the successful experiences from US to establish policies tailored to the status of pediatric drug development in order to promote pediatric drug development and improve the health level of Chinese children.
3.X-ray analysis of lung damage caused by PCl3 inhalation.
Chinese Journal of Industrial Hygiene and Occupational Diseases 2005;23(4):308-309
Adult
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Chlorides
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toxicity
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Female
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Humans
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Inhalation Exposure
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Lung
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diagnostic imaging
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Lung Injury
;
diagnostic imaging
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Male
;
Middle Aged
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Phosphorus Compounds
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toxicity
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Radiography
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Young Adult
5.Investigation on Phthalates in Drinking Water of Nantong City
Chun WANG ; Xiaodong LI ; Zili YANG
Journal of Environment and Health 1989;0(06):-
Objective To know phthalates(PAEs) pollution in drinking water of Nantong City. Methods With solid phase extraction and gas chromatography-mass spectroscope(GC-MS),main phthalates in drinking water were determined. Results Phthalates could be detected in all the source water, product water and tap water samples, mainly were di-n-butyl phthalate(DBP) and di(2-ethylhexyl) phthalate(DEHP),ranged from 1.25 to 4.59 ?g/L and from 0.97 to 3.57 ?g/L respectively. PAEs levels in product water were less than those in source water. The highest removal rate of DBP was 50%. PAEs levels in tap water were higher than those in product water,sometimes even higher than those in source water. Conclusion PAEs can not be effectively removed from source water after general water treatment process.
6.Cloning and Expression of EGFR Tyrosine Kinase and Construction of the Screening Model for the Enzymatic Inhibitors
Yang ZHANG ; Chun-Ping LIU ; Yuan LI ;
China Biotechnology 2006;0(10):-
As a member of tyrosine kinases(TK) family,Epidermal growth factor receptor(EGFR) has an activity of intrinsic protein tyrosine kinase,which plays an essential role in the regulation of signal transduction in the cells.Due to the abnormal expression of EGFR-TK,the certain type cancers may developed and progressed.Based on that,the inhibitors of EGFR-TK could be effective medicines for the treatment of cancer.The EGFR-TK domain was amplified by RT-PCR with RNA of HUVCEs cells as the template and expressed in E.coliBL21(DE3) using plasmid pET30a as vector.The recombinant protein was purified with the affinity chromatography(Ni-NTA),which was identified to have kinase activity catalyzing the substrate phosphorylated with ATP in the enzymatic reaction.Using the recombinant EGFR-TK as target,the screening model for enzymatic inhibitors was constructed.
9.Study of IL-3 and other hematopoietic cytokines expression of human amnion-derived mesenchymal stem cells and its significance
Chun YANG ; Jian YANG ; Mingxia SHI ; Zhi ZHOU ; Weijia LI
Journal of Leukemia & Lymphoma 2010;19(12):732-734,738
Objective To investigate the cytokine spectrum of cultured human amniotic-derived mesenchymal stem cells (A-MSC) for understanding its basis of molecular biology in hematopoietic in vitro.Methods Their hematopoietic cytokines expression was analyzed using RT-PCR in the mRNA level. Results It showed that in vitro subcultured human amniotic-derived mesenchymal stem cells were capable to express many important hematopoietic cytokines such as LIF, SCF, M-CSF, G-CSF, GM-CSF, IL-3, IL-6, IL-11 and so on. Conclusion Production of abundant of hematopoietic cytokines by human amniotic-derived mesenchymal stem cells may be effective for hematopoietic support and HSC transplantation.
10.Association of TNF gene polymorphism with Helicobacter pylori infection in Chinese patients with gastroduodenal diseases
Chun LI ; Bing XIA ; Yi YANG ; Jin LI ; Huaxiang XIA
Chinese Journal of Immunology 2000;0(11):-
Objective:To determine the di-allelic polymorphisms of TNF gene and their association with Helicobacter Pylori(H.pylori) infection and gastroduodenal diseases in Chinese population with Han nationality.Methods:Two hundreds and ten patients with gastroduodenal diseases(73 chronic gastritis,78 duodenal ulcer and 59 noncardia gastric cancer) and 264 healthy controls were genotyped by the PCR-RFLP method for TNF-? 308,lymphotoxin-?(LT-?) Nco Ⅰ and AspH Ⅰ gene polymorphisms.H.pylori infection status was determined by a validated serological test.Results:H.pylori infection was detected in 90.5% of 210 patients and 62.1% of 264 healthy controls(P